Fourier Shell Correlation (FSC) server
Welcome to the PDBe Fourier Shell Correlation (FSC) server!
This web service will calculate and plot the FSC curve from two 3D reconstructions that you upload.
You will be able to download the FSC curve as an XML file that can then be uploaded
as a part of a deposition to EMDB. This server uses the EMAN2
package
for the FSC calculation. The implementation of the 3 sigma and half bit threshold
curves is based on Imagic code kindly provided by Michael Schatz (ImageScience
Software GmbH).
Supported input file formats
include CCP4/MRC, Imagic, Spider,
EMAN2 HDF5 and others. We have one test data sets that you can use to try out the service
here. We appreciate your continuing
support and
feedback!
The uploaded maps will be deleted after the calculation is completed.
Quick links
Recent Entries
(Show all)
CryoEM structure of dimeric quinol dependent nitric oxide reductase from Neisseria meningitidis
Cryo-EM structure of MsRv1273c/72c from Mycobacterium smegmatis in the ADP-bound IFasym-3 (peptidisc) state (ADP 4degrees C treated)
Cryo-EM structure of Arabidopsis thaliana H2A-nucleosome with 147bp Widom 601 DNA (C2 symmetry)
Cryo-EM structure of Arabidopsis thaliana H2A.W-nucleosome with Arabidopsis native 147bp DNA 15.2.2 (C1 symmetry)
Molecular Architecture of Human Glycogen Debranching Enzyme: Insights into Glycogen Storage Disease III Pathogenesis
Cryo-EM structure of Arabidopsis thaliana H2A.W-nucleosome with Arabidopsis native 147bp DNA 15.2.2 (C2 symmetry)
Cryo-EM structure of Arabidopsis thaliana H2A.Z-nucleosome with Arabidopsis native 147bp DNA 15.2.2 (C1 symmetry)
4-start helix structure of SBA/Azo3GN3N3 microtube with a diameter of about 32 nm
Cryo-EM structure of Arabidopsis thaliana H2A-nucleosome with Arabidopsis native 147bp DNA 15.2.2 (C2 symmetry)
Cryo-EM structure of Arabidopsis thaliana H2A.Z-nucleosome with 147bp Widom 601 DNA (C2 symmetry)
Cryo-EM structure of Arabidopsis thaliana H2A.Z-nucleosome with 147bp Widom 601 DNA (C1 symmetry)
Cryo-EM structure of Arabidopsis thaliana H2A.Z-nucleosome with Arabidopsis native 147bp DNA 15.2.2 (C2 symmetry)
Cryo-EM structure of an TEF30-associated intermediate PSII core complex from Chlamydomonas reinhardtii
Cryo-EM structure of Arabidopsis thaliana H2A-nucleosome with 147bp Widom 601 DNA (C1 symmetry)
Cryo-EM structure of Arabidopsis thaliana H2A-nucleosome with Arabidopsis native 147bp DNA 15.2.2 (C1 symmetry)
Cryo-EM structure of a TEF30-associated intermediate PSII core dimer complex, type I, from Chlamydomonas reinhardtii
helix structure of SBA/S3GN microtube with a diameter of about 30 nm
60S ribosomal subunit in complex with E3-UFM1 ligase and RQC machinery components NEMF and LTN1 (LTN1)
RNA polymerase-sigma54-DNA focussed map of bacterial transcription intermediate complex mediated by activator PspF containing nifH promoter DNA containing mismatch from -10 to -1 - open clamp
Cryo-EM structure of human AK2 bound to reduced human AIFM1 (residues 102-613), class 1
Cryo-EM structure of the microbial rhodopsin CryoR1 at pH 4.3 in detergent
Human adult muscle nAChR in desensitised state in nanodisc with 100 uM acetylcholine
Single particle cryo-EM structure of the multidrug efflux pump MdtF from Escherichia coli
Cryo-EM structure of the microbial rhodopsin CryoR2 at pH 8.0 in detergent
Cryo-EM structure of the P domain of the Hepatitis E Virus ORF2 protein in complex with Fab ES4.431
60S ribosomal subunit in complex with E3-UFM1 ligase and RQC machinery components NEMF and LTN1 (Composite map)
60S ribosomal subunit in complex with RQC components NEMF and LTN1
60S ribosomal subunit in complex with E3-UFM1 ligase and RQC machinery components NEMF and LTN1 (60S body)
Cryo-EM structure of the microbial rhodopsin CryoR1 at pH 8.0 in detergent
Interface between baseplate cup and extended tail tube/sheath of Klebsiella phage KP1 variant vB_Kpn_Lilla1
Pre-assembled baseplate cup of Klebsiella phage KP1 variant vB_Kpn_Lilla1
Amyloid structure of 17kDa alpha-amylase/trypsin inhibitor 2 (Uniprot ID: AI172_ORYSJ)
Cryo-EM structure of the microbial rhodopsin CryoR1 at pH 10.5 in detergent in the ground state
Cryo-EM structure of the P domain of the Hepatitis E Virus ORF2 protein in complex with Fab ES1.327
Cryo-EM structure of the microbial rhodopsin CryoR1 at pH 8.0 in nanodisc
Cryo-EM recostruction of the complex between Rocahepevirus ratti (HEV rat) ORF2 protein in complex with Fab of antibody Es5.127
Pseudomonas aeruginosa polynucleotide phosphorylase in complex with recognition site of RNase E
Human adult muscle nAChR in resting state in nanodisc with alpha-bungarotoxin
CryoEM structure of the Elp-Hdr complex of Methanothermobacter marburgensis state 1 (composite structure)
Cryo-EM structure of human AK2 bound to reduced human AIFM1 (residues 102-613), class 2
Human adult muscle nAChR in resting state in detergent with alpha-bungarotoxin
CryoEM structure of the Hdr(ABC)2 subunits of the Elp-Hdr complex of Methanothermobacter marburgensis
CryoEM structure of the Elp-Hdr complex of Methanothermobacter marburgensis state 2 (composite structure)
Single particle cryo-EM structure of MdtF V610F with bound Rhodamine 6G
60S ribosomal subunit in complex with E3-UFM1 ligase, NEMF, and Sec61
RNA polymerase-sigma54-DNA subtracted focussed map of bacterial transcription intermediate complex mediated by activator PspF containing nifH promoter DNA containing mismatch from -10 to -1 - closed clamp
Cryo-EM structure of the microbial rhodopsin CryoR1 at pH 10.5 in detergent in the M state
Cryo-EM structure of human AK2 bound to reduced human AIFM1 (residues 102-613), class 3
60S ribosomal subunit in complex with E3-UFM1 ligase and RQC machinery components NEMF and LTN1 (UFM1 E3 ligase and NEMF)
Human adult muscle nAChR in desensitised state in nanodisc with 1 mM acetylcholine
Structure of the 50S ribosomal subunit from the antibiotic-producing bacterium Streptomyces fradiae
60S ribosomal subunit in complex with E3-UFM1 ligase and RQC machinery components NEMF and LTN1 (Consensus refinement map)
CryoEM structure of the Elp-Hdr complex of Methanothermobacter marburgensis state 2, dimer (composite structure)
Cryo-EM structure of the human NHA2-Fab complex bound to phloretin
Cryo-EM structure of full length Neuroligin-2 from Mouse bound to two Neurexin-1 Beta conformation one
Cryo-EM structure of full length Neuroligin-2 from Mouse bound to two Neurexin-1 Beta conformation two
Cryo-EM density map of HKU1 spike glycoprotein D1 domain in complex with 9O-acetyl GD3 sialoglycan (Active state, locally refined)
Consensus map of the human sweet taste receptor (TAS1R2 + TAS1R3) from the sucralose dataset
Human sweet taste receptor composed of TAS1R2 and TAS1R3 GPCRs from the combined datasets
Focused refinement map of WD40:ARM/ANK interface from LRRK2(I2020T) MLi-2 dataset
Human sweet taste receptor (TAS1R2 + TAS1R3) from the sucralose dataset
Local refinement map of the human sweet taste receptor lower-half of the Cysteine-Rich domains (CR) and transmembrane domains (TMD) from the PEG400 dataset
Cryo-EM structure of HCoV-HKU1 glycoprotein in complex with 9O-acetyl GD3 sialoglycan (UUU state)
Local refinement map of the human sweet taste receptor Venus Fly Trap domains (VFT) and upper-half of the Cysteine-Rich domains (CR) from the sucralose dataset
Cryo-EM structure of HCoV-HKU1 spike glycoprotein in complex with 9OAc-GD3 tetrasacchride (Deletion 33Pro34Arg)
CryoEM Structure of HCA2 DREADD Gi1 in complex with FCH-2296413 (Local Refinement)
Local refinement map of the human sweet taste receptor Venus Fly Trap domains (VFT) and upper-half of the Cysteine-Rich domains (CR) from the combined datasets
Human sweet taste receptor (TAS1R2 + TAS1R3) VFT domains from the combined datasets
C-terminus truncated (last two residues) mutant of Human light chain ferritin reacted with iron (3 Fe2+ to ferritin monomer ratio). Reconstruction of particles with no nanoparticle.
NP subunits next to the polymerase at the end of influenza D virus RNP, piece 2
Cryo-EM structure of human heteromeric Glycine Receptor alpha3S346E-beta with glycine
Cryo-EM structure of HCoV-HKU1 glycoprotein in complex with 9O-acetyl GD3 sialoglycan (DDA state)
Cryo-EM structure of human heteromeric Glycine Receptor alpha3S346E-beta in presence of glycine and 2,6-DTBP
Local refinement map of the human sweet taste receptor lower-half of the Cysteine-Rich domains (CR) and transmembrane domains (TMD) from the sucralose dataset
E. coli acetyl-CoA carboxylase, wide stacked local reconstruction, 3.20 Angstrom
The nucleoprotein subunits next the polymerase in the middle of influenza D RNP, piece 2
Human sweet taste receptor (TAS1R2 + TAS1R3) from the PEG400 dataset
Cryo-EM Structure of full length Neuroligin-2 from mouse with Neurexin-1 beta
Cryo-EM structure of HCoV-HKU1 glycoprotein in complex with 9O-acetyl GD3 sialoglycan (DAA state)
Cryo-EM structure of the trimeric SenDRT9 RT-ncRNA complex (GST fusion)
Cryo-EM structure of HCoV-HKU1 glycoprotein (mutant T31VPR34 to GGGG)
Cryo-EM structure of HCoV-HKU1 glycoprotein in complex with 9O-acetyl GD3 sialoglycan (DAU state)
SARS-CoV-2 Nucleocapsid Dimerization Domain bound to Fab-NP1E9 and Fab-NP3B4
Human sweet taste receptor (TAS1R2 + TAS1R3) VFT domains bound to sucralose
NP subunits next to the polymerase at the end of influenza D virus RNP, piece 1
Consensus map of the Human sweet taste receptor (TAS1R2 + TAS1R3) from the aspartame dataset
Cryo-EM structure of HCoV-HKU1 glycoprotein D1 Domain (mutant T31VPR34 to GGGG)
Cryo-EM structure of HCoV-HKU1 glycoprotein D1 Domain (Deletion 33Pro34Arg)
Cryo-EM structure of HCoV-HKU1 glycoprotein D1 (Down State, locally refined)
Cryo-EM density map of HKU1 spike glycoprotein D1 domain in complex with 9O-acetyl GD3 sialoglycan (Down_alt state, locally refined)
Transmembrane domains of the human sweet receptor (TAS1R2 + TAS1R3) from Class 3 particles (rigidly fitted from PDB:9NOX and 9NOR)
Structure of a native Drosophila melanogaster Pol II Elongation Complex without Rpb4/Rpb7 stalk
Human light chain ferritin reacted with iron (3 Fe2+ to ferritin monomer ratio). Reconstruction of particles with one nanoparticle.
Structure of the loop end of influenza D virus RNP complex, class 2
Consensus map of the human sweet taste receptor composed of TAS1R2 and TAS1R3 GPCRs from the combined datasets
The nucleoprotein subunits next the polymerase in the middle of influenza D RNP, piece 1
Consensus map of the human sweet taste receptor (TAS1R2 + TAS1R3) from the PEG400 dataset
C-terminus truncated (last two residues) mutant of Human light chain ferritin reacted with Ferrous salt(3 Fe2+ per ferritin subunit) . Reconstruction of particles with one nanoparticle.
Cryo-EM density map of HKU1 spike glycoprotein D1 domain in complex with 9O-acetyl GD3 sialoglycan (Up state, locally refined)
Cryo-EM Structure of HKU1 spike D1 Domain (Active state, locally refined)
Cryo-EM structure of HCoV-HKU1 glycoprotein in complex with 9O-acetyl GD3 sialoglycan (AUU state)
Human sweet taste receptor (TAS1R2 + TAS1R3) VFT domains bound to aspartame
Local refinement map of the human sweet taste receptor lower-half of the Cysteine-Rich domains (CR) and transmembrane domains (TMD) from the combined datasets
Local refinement map of the human sweet taste receptor Venus Fly Trap domains (VFT) and upper-half of the cysteine-rich domains (CR) from the PEG400 dataset
Cryo-EM structure of HCoV-HKU1spike glycoprotein D1 Domain in complex with 9OAc-GD3 tetrasacchride (Deletion 33Pro34Arg)
Cryo-EM structure of HCoV-HKU1 glycoprotein in complex with 9OAc-GD3(mutant T31VPR34 to GGGG)
Human light chain ferritin reacted with iron (3 Fe2+ to ferritin monomer ratio). Reconstruction of particles with no nanoparticle.
Cryo-EM structure of human Glycine Receptor apha3-beta heteromer with glycine in nanodisc
Cryo-EM structure of extracellular tube from Microcystis Aeruginosa pcc 7806SL
Full-length autoinhibited LRRK2(I2020T) co-polymerized with microtubules and MLi-2
Transmembrane domains of the human TAS1R2 sweet receptor subunit in complex with miniGs/gust25
Cryo-EM structure of full length Neuroligin-2 from Mouse bound to two Neurexin-1 Beta conformation three
Cryo-EM structure of human Glycine Receptor alpha3-beta heteromer in presence of glycine
Cryo-EM structure of HCoV-HKU1 glycoprotein (Deletion 33Pro34Arg)
Subtomogram averaging of the polymerase end of influenza RNP complex
Cryo-EM structure of HCoV-HKU1 glycoprotein D1 Domain in complex with 9OAc-GD3(mutant T31VPR34 to GGGG)
Cryo-EM structure of CT-BCCP domain of Pyruvate carboxylase from Mycobacterium tuberculosis
CryoEM structure of monomeric quinol dependent nitric oxide reductase from Neisseria meningitidis
Cryo-EM structure of MsRv1273c/72c(E553Q) mutant from Mycobacterium smegmatis in the ATP-bound Occ state
Cryo-EM structure of MsRv1273c/72c from Mycobacterium smegmatis in the ATP|ADP-bound IFasym-2 state
Cryo-EM structure of Streptomyces coelicolor sigma factor shbA transcription initiation complex
Cryo-EM structure of Streptomyces coelicolor sigma factor shbA transcription initiation complex with shbA promoter
Cryo-EM structure of MsRv1273c/72c from Mycobacterium smegmatis in the ADP-bound IFasym-3 state (ADP 4 degrees C treated)
CryoEM structure of Adenovirus serotype 3 premature hexon in complex with Adenovirus serotype 2 100K
Cryo-EM structure of MsRv1273c/72c from Mycobacterium smegmatis in the ATP|ADP+Vi-bound Occ (Vi) state
F1 domain of Non-catalytic site depleted and epsilon C-terminal domain deleted FoF1-ATPase from Bacillus PS3,under ATP saturated condition
Cryo-EM structure of MsRv1273c/72c from Mycobacterium smegmatis in the ADP-bound IFasym-3 (peptidisc) state (ATP 37degrees C treated)
Cryo-EM structure of H5 hemagglutinin from human-infecting H5N8 influenza virus in complex with Sialyl-Lewis X
Cryo-EM structure of MsRv1273c/72c from Mycobacterium smegmatis in the ADP-bound IFasym-3 state (ATP 37 degrees C treated