Fourier Shell Correlation (FSC) server
Welcome to the PDBe Fourier Shell Correlation (FSC) server!
This web service will calculate and plot the FSC curve from two 3D reconstructions that you upload.
You will be able to download the FSC curve as an XML file that can then be uploaded
as a part of a deposition to EMDB. This server uses the EMAN2
package
for the FSC calculation. The implementation of the 3 sigma and half bit threshold
curves is based on Imagic code kindly provided by Michael Schatz (ImageScience
Software GmbH).
Supported input file formats
include CCP4/MRC, Imagic, Spider,
EMAN2 HDF5 and others. We have one test data sets that you can use to try out the service
here. We appreciate your continuing
support and
feedback!
The uploaded maps will be deleted after the calculation is completed.
Quick links
Recent Entries
(Show all)
Structure of SARS-CoV-2 Orf3a in late endosome/lysosome-like membrane environment, MSP1D1 nanodisc
Structure of SARS-CoV-1 Orf3a in late endosome/lysosome-like environment, MSP1D1 nanodisc
Single-Molecule 3D Image of 16 Helix RNA Origami Satellite by Individual Particle Electron Tomography (No. 11)
Cryo-EM structure of SARS-CoV-2 Omicron BA.2 spike protein in complex with mouse ACE2
Negative stain EM map of SARS-CoV-2 Spike in complex with CoVIC-045
Single-Molecule 3D Image of 16 Helix RNA Origami Satellite by Individual Particle Electron Tomography (No. 13)
Cryo-EM structure of SARS-CoV-2 Omicron BA.1 spike protein in complex with mouse ACE2 (focused refinement of RBD and ACE2)
CryoEM structure of Conalbumin from chicken egg white (sigma-Cas 1391-06-6)
Cryo-EM structure of SARS-CoV-2 Omicron BA.2 spike protein in complex with human ACE2
Structure of SARS-CoV-2 Orf3a in late endosome/lysosome-like environment, Saposin A nanodisc
Single-Molecule 3D Image of 16 Helix RNA Origami Satellite by Individual Particle Electron Tomography (No. 15)
Cryo-EM structure of a Zinc-loaded D287A mutant of the YiiP-Fab complex
Cryo-EM structure of SARS-CoV-2 Omicron BA.2 spike protein in complex with mouse ACE2 (focused refinement of RBD and ACE2)
Single-Molecule 3D Image of 16 Helix RNA Origami Satellite by Individual Particle Electron Tomography (No. 08)
Bovine complex I in the active state at 3.1 A (Proximal hydrophobic domain)
Bovine complex I in the presence of IM1761092, deactive class i (Proximal hydrophobic domain)
Cryotomogram of endolysin Ply006 treated Listeria monocytogenes Rev2 cell (stage 2)
Tail tip of siphophage T5 : open cone after interaction with bacterial receptor FhuA
Bovine complex I in the presence of IM1761092, slack class ii (Consensus)
T5 Receptor Binding Protein pb5 in complex with its E. coli receptor FhuA
Cryotomogram of endolysin Ply007 treated Enterococcus faecalis cells
Bovine complex I in the presence of IM1761092, deactive class vi (Consensus)
Bovine complex I in the presence of IM1761092, slack class ii (Proximal hydrophobic domain)
Bovine complex I in the presence of IM1761092, deactive class vi (Proximal hydrophobic domain)
Native Chemotaxis Core Signalling Complex from E. coli, with focused alignment on the baseplate (CheA-CheW)
Cryo-EM structure of Mycobacterium tuberculosis RNA polymerase holoenzyme dimer comprising sigma factor SigB, conformation 2
Bovine complex I in the presence of IM1761092, deactive class iv (Hydrophilic domain)
Bovine complex I in the presence of IM1761092, deactive class iii (Proximal hydrophobic domain)
Bovine complex I in the presence of IM1761092, active class iii (Proximal membrane domain)
Bovine complex I in the presence of IM1761092, active class i (Composite map)
Bovine complex I in the presence of IM1761092, active class iii (Distal membrane domain)
Bovine complex I in the presence of IM1761092, active class iv (Distal membrane domain)
Bovine complex I in the presence of IM1761092, deactive class vi (Distal hydrophobic domain)
Cryotomogram of endolysin Ply006 treated Listeria monocytogenes Rev2 cell (stage 3)
Cryo-EM structure of the canine distemper virus tetrameric attachment glycoprotein
Bovine complex I in the presence of IM1761092, deactive class ii (Distal membrane domain)
Bovine complex I in the presence of IM1761092, deactive class vi (Composite map)
Bovine complex I in the presence of IM1761092, active class i (Distal hydrophobic domain)
Native Chemotaxis Core Signalling Complex from E. coli, Focused alignment on ligand binding domain
Bovine complex I in the presence of IM1761092, active class ii (Composite map)
Structure of the Native Chemotaxis Core Signalling Complex from E-gene lysed E. coli cells.
Cryotomogram of endolysin Ply007 treated Enterococcus faecalis cells
Bovine complex I in the presence of IM1761092, deactive class i (Distal hydrophobic domain)
Bovine complex I in the presence of IM1761092, slack class i (Distal hydrophobic domain)
Bovine complex I in the presence of IM1761092, deactive class iv (Composite map)
Bovine complex I in the presence of IM1761092, deactive class iii (Hydrophilic domain)
Bovine complex I in the presence of IM1761092, slack class i (Composite map)
Structure of a yeast 80S ribosome-bound N-Acetyltransferase B complex
Bovine complex I in the presence of IM1761092, active class ii (Proximal hydrophobic domain)
Bovine complex I in the presence of IM1761092, deactive class ii (Consensus)
Bovine complex I in the presence of IM1761092, slack class i (Consensus)
Bovine complex I in the active state at 3.1 A (Distal hydrophobic domain)
Bovine complex I in the presence of IM1761092, deactive class iii (Composite map)
Bovine complex I in the presence of IM1761092, deactive class iv (Proximal hydrophobic domain)
Bovine complex I in the presence of IM1761092, active class iv (Consensus)
Bovine complex I in the presence of IM1761092, deactive class ii (Composite map)
Bovine complex I in the presence of IM1761092, deactive class ii (Hydrophilic domain)
Bovine complex I in the presence of IM1761092, deactive class iv (Consensus)
Bovine complex I in the presence of IM1761092, deactive class ii (Proximal membrane domain)
Bovine complex I in the presence of IM1761092, slack class i (Proximal hydrophobic domain)
Bovine complex I in the presence of IM1761092, active class iii (Consensus)
Bovine complex I in the presence of IM1761092, deactive class iv (Distal hydrophobic domain)
Bovine complex I in the presence of IM1761092, active class iii (Hydrophilic domain)
Bovine complex I in the presence of IM1761092, deactive class iii (Distal hydrophobic domain)
Cryo-EM structure of the Arabidopsis thaliana I+III2 supercomplex (Conformation 1 membrane arm)
Cryotomogram of individual Efs7 virions and phage attachment to a Enterococcus faecalis cell
Bovine complex I in the presence of IM1761092, active class i (Consensus)
Bovine complex I in the presence of IM1761092, active class iv (Composite map)
Cryo-EM Snapshots of Nanodisc-Embedded Native Eukaryotic Membrane Proteins
Bovine complex I in the presence of IM1761092, active class ii (Hydrophilic domain)
Bovine complex I in the presence of IM1761092, active class iv (Hydrophilic domain)
Bovine complex I in the presence of IM1761092, active class i (Hydophilic domain)
Bovine complex I in the presence of IM1761092, deactive class i (Hydrophilic domain)
Bovine complex I in the presence of IM1761092, deactive class iii (Consensus)
1.85 angstrom resolution cryo-EM reconstruction of tobacco mosaic virus, imaged on a JEOL CryoARM 300 with Direct Electron Apollo camera.
Bovine complex I in the presence of IM1761092, deactive class v (Composite map)
Bovine complex I in the presence of IM1761092, slack class ii (Composite map)
Cryotomogram of endolysin Ply007 treated Enterococcus faecalis cells
Bovine complex I in the presence of IM1761092, deactive class vi (Hydrophilic domain)
Structure of a yeast 80S ribosome-bound N-Acetyltransferase B complex
Bovine complex I in the presence of IM1761092, deactive class v (Proximal hydrophobic domain)
Cryo-EM structure of mouse Pannexin 1 purified in Salipro nanoparticles
Bovine complex I in the presence of IM1761092, active class ii (Consensus map)
Bovine complex I in the presence of IM1761092, deactive class v (Hydrophilic domain)
Cryotomogram of L-form-like cytoplasmic membrane vesicles of Listeria monocytogenes Rev2 cell
Bovine complex I in the presence of IM1761092, deactive class v (Distal hydrophobic domain)
Bovine complex I in the presence of IM1761092, active class i (Proximal hydrophobic domain)
Bovine complex I in the presence of IM1761092, deactive class i (Consensus)
Bovine complex I in the presence of IM1761092, slack class i (Hydrophilic domain)
Bovine complex I in the presence of IM1761092, active class iv (Proximal membrane domain)
Bovine complex I in the presence of IM1761092, deactive class v (Consensus)
Tail tip of siphophage T5 : full complex after interaction with its bacterial receptor FhuA
Bovine complex I in the presence of IM1761092, deactive class i (Composite map)
Cryo-EM structure of Mycobacterium tuberculosis RNA polymerase core
Bovine complex I in the presence of IM1761092, slack class ii (Distal hydrophobic domain)
Cryotomogram of a A006 virion attaching to a Listeria monocytogenes Rev2 cell
Protomeric substructure from an octameric assembly of M. tuberculosis RNA polymerase in complex with sigma-b initiation factor
Tail tip of siphophage T5 : bent fibre after interaction with its bacterial receptor FhuA
Cryotomogram of endolysin Ply006 treated Listeria monocytogenes Rev2 cell (stage 1)
Bovine complex I in the presence of IM1761092, slack class ii (Hydrophillic domain)
M. tuberculosis RNA polymerase in complex with sigma-b initiation factor: octameric assembly of (alpha)2-beta-beta'-omega-sigB protomers.
Cryo-EM structure of the whole photosynthetic complex from the green sulfur bacteria
Cryo-EM structure of Mycobacterium tuberculosis RNA polymerase holoenzyme dimer comprising sigma factor SigB
Bovine complex I in the presence of IM1761092, active class iii (Composite map)
Bovine complex I in the presence of IM1761092, active class ii (Distal hydrophobic domain)
Single-Molecule 3D Image of 16 Helix RNA Origami Satellite by Individual Particle Electron Tomography (No. 05)
Single-Molecule 3D Image of 16 Helix RNA Origami Satellite by Individual Particle Electron Tomography (No. 04)