About EMDB
Meet the team
The Electron Microscopy Data Bank (EMDB) team operates out of EMBL-EBI and you can read more about us here.
EMDB history
The EMDB is a public repository for cryogenic-sample Electron Microscopy (cryoEM) volumes and representative tomograms of macromolecular complexes and subcellular structures. It covers a variety of techniques, including single-particle analysis, helical reconstruction, electron tomography, subtomogram averaging, and electron crystallography (for more information, see the EMDB Policies).
EMDB was founded at EMBL-EBI in 2002 under the leadership of Kim Henrick. From 2007, the archive has been operated jointly by EMBL-EBI and the Research Collaboratory for Structural Bioinformatics (RCSB)a. In 2013, the Protein Data Bank Japan (PDBj) also became involved in EMDB. As of the first of January, 2021, EMDB is an archive operated under the aegis of the Worldwide Protein Data Bank (wwPDB), and has also joined that organisation as a full member.
Results of cryo-EM studies are archived in three collaborating archives:
- EMDB stores the processed 3D volumes and tomograms;
- PDB stores any atomic models that have been constructed based on EMDB data;
- EMPIAR stores the raw EM data underpinning the data in EMDB/PDB.
Data for EMDB and PDB can be deposited through the wwPDB deposition and annotation system OneDep.
EMPIAR (Electron Microscopy Public Image Archive) is a companion archive to EMDB, founded at and operated by EMBL-EBI, and with a mirror site at PDBj.
Citing EMDB
Please cite the following publication in your papers and on websites: The wwPDB Consortium, EMDB—the Electron Microscopy Data Bank, Nucleic Acids Research, Volume 52, Issue D1, 5 January 2024, Pages D456–D465, https://doi.org/10.1093/nar/gkad1019
To cite individual entries, please quote the EMDB accession code(s) and cite the original publication(s) in which the entries are described.
EMDB logos and branding should not be used on public-facing websites unless explicit prior permission has been obtained from us. However, if you want to use an EMDB logo in a presentation or report, you can use the images in the following table royalty-free and without the need to obtain prior permission from us.
More information
Please see our FAQ, Policies, description of the EMDB data model, or contact the EMDB helpdesk.
Funding
The work on EMDB at EMBL-EBI has been funded in the past by the European Commission, NIH, UKRI-BBSRC and EMBL-EBI (through the EMBL member states). It is currently funded by the Wellcome Trust and EMBL-EBI.
Quick links
Recent Entries
(Show all)Cryo-EM structure of Pol epsilon-PCNA reconstituted on a DNA scaffold with 5'-overhangs on either side of the double-stranded segment
Lectin FRIL from Lablab purpureus complexed to Lewis X tetrasaccharide
Focused refinement of 19S in the substrate-engaged human 26S proteasome bound to midnolin with RPT1 at top of spiral staircase
Focused refinement of 19S in the substrate-engaged human 26S proteasome bound to midnolin with RPT5 at top of spiral staircase
Focused refinement of 19S in the substrate-engaged human 26S proteasome bound to midnolin with RPT2 at top of spiral staircase
Anthoceros agrestis Rubisco (8 RbcL and 8 RbcS1) head to head stacking.
SARS-CoV-2 spike S2 trimer stabilized in the early fusion intermediate conformation bound to C77G12 (S2 local refinement)
Consensus map of substrate-engaged human 26S proteasome bound to midnolin with RPT1 at top of spiral staircase
pseudorabies virus (PRV) structure reconstructed from elastically scattered electrons using Cc-corrected cryo-EM
Cryo-EM structure of eSaCas9-NNG-guide RNA-mismatched target DNA complex
Cryo-EM structure of wild-type SaCas9-guide RNA-mismatched target DNA complex
Structure of flagellar hook at 3.50 angstroms resolution,conformation 3.
Consensus map of substrate-engaged human 26S proteasome bound to midnolin with RPT5 at top of spiral staircase
Structure of flagellar hook at 3.14 angstroms resolution,conformation 2.
Consensus map of substrate-engaged human 26S proteasome bound to midnolin with RPT2 at top of spiral staircase
Structure of flagellar hook at 3.18 angstroms resolution,conformation 1.
Cryo-EM structure of EBV gp350 D123 in complex with neutralizing antibody 4A11
Cryo-EM structure of EBV gp350 D123 in complex with neutralizing antibody 1A12 and 1H5 and non-neutralizing antibody 2E9
Structure of Norrin in complex with human Tspan12 large extracellular loop (Tspan12 LEL)
Cryo-EM structure of the 48-nm repeat doublet microtubule from Tekt5 KO mouse sperm
Cryo-EM structure of the 48-nm repeat doublet microtubule from Tssk6 KO mouse sperm
local ATPase-NCP density map of the ncBAF-nucleosome complex in the ADP-BeFx-bound state
Cryo-EM structure of the 48-nm repeat doublet microtubule from Tekt1 KO mouse sperm
Cryo-EM structure of the 48-nm repeat doublet microtubule from Dusp21 KO mouse sperm
Heptamer Msp1 from S.cerevisiae (with a catalytic dead mutation) in complex with an unknown peptide substrate
cryoEM map of Apo Aspergillus fumigatus acetolactate synthase (ALS)
cryoEM structure of Aspergillus fumigatus acetolactate synthase (ALS) in complex with a novel inhibitor
Plasmodium falciparum gametocyte microtubule with 15 protofilaments determined in situ
State 2 MAP 3 RNA Pol II activated elongation complex with SETD2 bound to proximal upstream H3
Cryo-EM structure of cardiac amyloid fibril from a variant apolipoprotein A-I R173P amyloidosis patient
Cryo-EM structure of renal amyloid fibril from a variant apolipoprotein A-I R173P amyloidosis patient
50S focus refined map of E.coli 70S ribosome complexed with P-site fMet-tRNAfMet and A-site S-beta(2)hydroxyBocK-tRNAPyl
70S global refined map of E.coli 70S ribosome complexed with P-site fMet-tRNAfMet and A-site R-beta(2)hydroxyBocK-tRNAPyl
amyloid fibril of recombinant full-length 2N4R tau complexed with unfractionated mouse liver RNA and seeded by Alzheimer's disease tau fibrils
30S focus refined map of E.coli 70S ribosome complexed with P-site fMet-tRNAfMet and A-site S-beta(2)hydroxyBocK-tRNAPyl
amyloid fibril of recombinant full-length 2N4R tau complexed with mouse liver 18S ribosomal RNA
Cryo-EM structure of cardiac amyloid fibril from a variant apolipoprotein A-I L90P amyloidosis patient
Homomeric Glycine Receptor alpha2 with 1 mM Glycine in an Open State
Homomeric Glycine Receptor alpha2 with 1 mM Glycine in a Desensitized State
Homomeric Glycine Receptor alpha2 with 0.1 mM Glycine in a Desensitized State
Homomeric Glycine Receptor alpha2 with 0.1 mM Glycine in an Open State
Homomeric Glycine Receptor alpha2 with PTX in a Desensitized State
Homomeric Glycine Receptor alpha2 with 0.1 mM Glycine in an Apo State
Homomeric Glycine Receptor alpha2 with 1 mM Glycine in a Closed State
human ZYG11B ElonginB/C complex binding to SARS-CoV2 Orf10 protein
Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES
Structure of WT E.coli ribosome 70S subunit with complexed with mRNA, P-site fMet-NH-tRNAfMet and A-site (S)-betahydroxyBocK charged NH-tRNAPyl
Structure of WT E.coli ribosome 70S subunit with complexed with mRNA, P-site fMet-NH-tRNAfMet and A-site (R) beta-2-hydroxy-BocLysine acid charged NH-tRNAPyl
Subtomogram Averaged Cryo-ET Structure of the 96-nm Axonemal Repeat in RSP3A-HA-BCCP Tetrahymena thermophila
Subtomogram Averaged Cryo-ET Structure of the 96-nm Axonemal Repeat in RSP3A-KO Tetrahymena thermophila
Subtomogram Averaged Cryo-ET Structure of the 96-nm Axonemal Repeat in RSP3C-KO Tetrahymena thermophila
30S ribosomal subunit from E. coli missing the gene encoding for the 16S rRNA 2'-O-methyltransferase RsmI
Two protofilament structure of alpha1B and betaI/IVb microtubule bound to GMPCPP
Two protofilament structure of alpha1B and betaI/IVb microtubule bound to GDP
The primed conformation of herpes simplex virus type 1 (HSV-1) glycoprotein B (gB)
The prefusion conformation of herpes simplex virus type 1 (HSV-1) glycoprotein B (gB)
The primed conformation of herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) mutant H534F
The postfusion conformation of herpes simplex virus type 1 (HSV-1) glycoprotein B (gB)
The primed conformation of herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) mutant N511P
The prefusion conformation of herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) mutant S392C and A527C
Montaged cryo-ET map of individual particle 3D reconstructions of ~2 kb negatively supercoiled plasmids (delta Lk centered on -15) under near physiological salt condition (40 mM K+, 5 mM Mg2+) in the presence of transcribed RNA polymerase and dCas9
Montaged cryo-ET map of individual particle 3D reconstructions of ~2 kb negatively supercoiled plasmids (delta Lk centered on -15) under near physiological salt condition (40 mM K+, 5 mM Mg2+) with stalled RNA polymerase bound
Montaged cryo-ET map of individual particle 3D reconstructions of ~2 kb negatively supercoiled plasmids (delta Lk centered on -15) under near physiological salt condition (40 mM K+, 5 mM Mg2+) with dCas9 bound
Montaged cryo-ET map of individual particle 3D reconstructions of ~2 kb negatively supercoiled plasmids (delta Lk centered on -15) under near physiological salt condition (40 mM K+, 5 mM Mg2+) in the presence of stalled RNA polymerase and dCas9
Montaged cryo-ET map of individual particle 3D reconstructions of ~2 kb negatively supercoiled plasmids (delta Lk centered on -15) under low salt conditions (5 mM K+)
The deep-primed conformation of herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) mutant R131F L97E and L101E
Montaged cryo-ET map of individual particle 3D reconstructions of ~2 kb negatively supercoiled plasmids (delta Lk centered on -15) under near physiological salt condition (40 mM K+, 5 mM Mg2+) with RNA polymerase bound during active transcription
Montaged cryo-ET map of individual particle 3D reconstructions of ~2 kb negatively supercoiled plasmids (delta Lk centered on -15) under near physiological salt condition (40 mM K+, 5 mM Mg2+)
Montaged cryo-ET map of individual particle 3D reconstructions of ~2 kb negatively supercoiled plasmids (delta Lk centered on -15) under near physiological salt condition (40 mM K+, 5 mM Mg2+) in the presence of transcribed RNA polymerase and Topoisomerase I
The primed conformation of herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) mutant H516P
Cryo-EM structure of Sudan Ebolavirus GP bound by three neutralizing antibodies 316L, 523S and 294S
Cryo-EM structure of Sudan Ebolavirus GP bound by three neutralizing antibodies 545S, 523S and 294S
a5b3 GABAAR bound to Topiramate, GABA, and Mb25 in a desensitized state in saposin nanodiscs
a5b3 GABAAR bound to GABA, and Mb25 in a desensitized state in saposin nanodiscs, topiramate-free
Cryo-EM structure of SARS-CoV-2 RBD in complex with ACE2 and mAb 1C4
Structure of the small subunit of the flowering plant mitoribosome with the maturation factor RsgA
Cryo-EM structure of the freshwater actinorhodopsin, Rhodoluna lacicola (RlActR)
Cryo-EM structure of SARS-CoV-2 WT spike protein in complex with nAb 1C4
native cytoplasmic lattices from mouse oocytes, one asymmetric unit, consensus refinement
native cytoplasmic lattices from mouse oocytes, one asymmetric unit, local refinement of PADI6 region
native cytoplasmic lattices from mouse oocytes, one asymmetric unit, local refinement of SCMC region
CryoEM structure of human DNMT1 (aa 698-1616) bound to hemimethylated dsDNA and Inhibitor DMI26
native cytoplasmic lattices from mouse oocytes, one asymmetric unit, local refinement of tubulin region
Evaluating the Volta Phase Plate for Improved Tomogram Alignment in Cryo-Electron Tomography: structure of 70S ribosome without VPP (full dataset)
Evaluating the Volta Phase Plate for Improved Tomogram Alignment in Cryo-Electron Tomography: structure of 80S ribosome without VPP (full dataset)
Evaluating the Volta Phase Plate for Improved Tomogram Alignment in Cryo-Electron Tomography: structure of 70S ribosome without VPP (partial dataset)
Evaluating the Volta Phase Plate for Improved Tomogram Alignment in Cryo-Electron Tomography: structure of 80S ribosome without VPP (partial dataset)
Evaluating the Volta Phase Plate for Improved Tomogram Alignment in Cryo-Electron Tomography: structure of PP7 virus-like-particle with VPP (partial dataset)
The structure of short splice variant (Q9UBL9-2) of human P2X2 receptor channel in lipid nanodiscs
The pre-open state 1 structure of human P2X2 receptor channel in lipid nanodiscs with free ATP and sodium
The open state structure of human P2X2 receptor channel in lipid nanodiscs with free ATP and sodium
The pre-desensitized state structure of human P2X2 receptor channel in lipid nanodiscs with free ATP and sodium
Human P2X2 receptor channel in lipid nanodiscs with 0.5mM Suramin
The desensitized state structure of human P2X2 receptor channel in lipid nanodiscs with free ATP and sodium
The structure of short splice variant (Q9UBL9-2) of human P2X2 receptor channel in lipid nanodiscs with 5mM MgCl2
Human P2X2 receptor channel in detergent with residual ATP after apyrase treatment
The structure of short splice variant (Q9UBL9-2) of human P2X2 receptor channel in lipid nanodiscs with Mg-ATP
Fab-14/SARS-CoV-2 D614G spike complex, Mode II, subgroup I conformation
Fab-14/SARS-CoV-2 D614G spike complex, Mode II, subgroup II conformation
Fab-14/SARS-CoV-2 D614G spike complex, Mode IV, subgroup II conformation
Fab-14/SARS-CoV-2 D614G spike complex, Mode II, subgroup III conformation
Fab-14/SARS-CoV-2 D614G spike complex, Mode IV, subgroup II conformation
DNA Ligase IIIa bound to a nucleosome containing a nick at SHL-4 (composite)
DNA Ligase IIIa bound to a nucleosome containing a nick at SHL-6 (composite)
DNA Ligase IIIa bound to a nucleosome containing a nick at SHL-4 (consensus)
DNA Ligase IIIa bound to nucleosome containing a nick at SHL-2 (LigIIIa/DNA local refine)
XRCC1-DNA Ligase IIIa complex bound to a nucleosome containing a nick at SHL-6 (consensus)
DNA Ligase IIIa bound to a nucleosome containing a nick at SHL-6 (LigIIIa/DNA local refine)
DNA Ligase IIIa bound to a nucleosome containing a nick at SHL-6 (consensus)
DNA Ligase IIIa bound to nucleosome containing a nick at SHL-2 (consensus)
DNA Ligase IIIa bound to a nucleosome containing a nick at SHL-4 (LigIIIa/DNA local refine)
XRCC1-DNA Ligase IIIa complex bound to a nucleosome containing a nick at SHL-6 (composite)
DNA Ligase IIIa bound to nucleosome containing a nick at SHL-2 (composite)
XRCC1-DNA Ligase IIIa bound to a nucleosome containing a nick at SHL-6 (LigIIIa/DNA local refine)
Local refinement of Fab-14/SARS-CoV-2 D614G spike complex, Mode I, Subgroup I conformation
Pol II-DSIF-SPT6-PAF1c-TFIIS-IWS1-ELOF1-LEDGF-nucleosome LEDGF+nucleosome map Q
Local refinement of Fab-14/SARS-CoV-2 D614G spike complex, Mode I
Pol II-DSIF-SPT6-PAF1c-TFIIS-IWS1-ELOF1-LEDGF-nucleosome SPT6 N-terminus local map P
Structure of dimeric mouse NLRP14-KDM2A-SKP1 complex /Consensus Map
Structure of dimeric mouse NLRP14-KDM2A-SKP1 complex /Focused Map B
IL-33 and Etokimab fab and Tozorakimab fab ternary complex structure
IL-33 and Itepekimab fab and Tozorakimab fab ternary complex structure
Structure of dimeric mouse NLRP14-KDM2A-SKP1 complex /Focused Map A
Map D Locally refined map of ZSWIM8-CUL3 complex bound to AGO2-miR-7-CYRANO
Map C focused map of ZSWIM8-CUL3 complex bound to AGO2-miR-7-CYRANO
Map B Locally refined interactions of ZSWIM8-CUL3 complex bound to AGO2-miR-7-CYRANO
Map E Composite map of ZSWIM8-CUL3 complex bound to AGO2-miR-7-CYRANO
Molecular basis of ZPD homopolymerization: cryo-EM structure of a native vertebrate egg coat filament
Focused map of 4 subunits of heptameric Rep40-dsDNA (ITR) in complex with ATPgS
Focused map of 3 subunits of Rep40 +dsDNA (ITR) in complex with ATPgS
Consensus map of heptameric Rep40-dsDNA (ITR) in presence of ATPyS
Consensus map of Hexameric AAV2 Rep40-dsDNA (ITR) duplex complex in presence of ATPyS
Map of the human LRP2 ectodomain with reduced endogenous ligands occupancy and incubated with LRPAP1
Map of the human LRP2 ectodomain with reduced endogenous ligands occupancy
Cryo-EM structure of the CDK11B-cyclin L2-SAP30BP bound to OTS964 (conformation 1)
Membrane domain of Complex I from Ovis aries in presence of D1 inhibitor, Closed state
Consensus map of Complex I Ovis aries in presence of D1 inhibitor, Closed state
Ternary cryo-EM structure of human ALG9 with Dol25-PP-GlcNAc2Man8, Dol25-P-Man and Fab
Peripheral arm of Complex I from Ovis aries in presence of D1 inhibitor, Closed state
Ternary cryo-EM structure of human ALG9 with Dol25-PP-GlcNAc2Man6, Dol25-P-Man and Fab
Focused map of Complex I junction from Ovis aries in presence of D1 inhibitor, Closed state
Ternary cryo-EM structure of chicken ALG12 with Dol25-PP-GlcNAc2Man7, Dol25-P-Man, and Fab
Ternary cryo-EM structure of yeast ALG3 with Dol25-PP-GlcNAc2Man5, Dol25-P-Man, and Fab




