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Substrates for peptidase S01.233: plasmin

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Pharma

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate Uniprot Residue range Cleavage Site Cleavage type Evidence P4 P3 P2 P1 P1' P2' P3' P4' Reference CutDB MERNUM
Ac-Ala-Arg-Phe-Lys-ACC Ac-Ala-Arg-Phe-Lys+ACC S Ala Arg Phe Lys ACC - - - Gosalia et al., 2005
Ac-Ala-Arg-Tyr-Arg-ACC Ac-Ala-Arg-Tyr-Arg+ACC S Ala Arg Tyr Arg ACC - - - Gosalia et al., 2005
aggrecan core protein P16112 17-2415 peptide-Arg394+Gly-peptide P Gly Glu Ala Arg Gly Ser Val Ile Fosang et al., 1998 18239
alpha-2-antiplasmin P08697 24-402 peptide-Arg403+Met-peptide P Ala Met Ser Arg Met Ser Leu Ser Holmes et al., 1987 24033
alpha-2-macroglobulin P01023 24-1474 peptide-Arg719+Leu-peptide P Gly His Ala Arg Leu Val His Val Mortensen et al., 1981
anti-Mullerian hormone P03971 26-560 peptide-Arg451+Ser-peptide P Arg Ala Gln Arg Ser Ala Gly Ala Maggard et al., 1996 18834
arginyl bond peptide-Arg+peptide N - - NPe Arg CPe - - - Castellino, 2004
beta-2-glycoprotein 1 P02749 20-345 peptide-Lys336+Thr-peptide P Ala Phe Trp Lys Thr Asp Ala Ser Ohkura et al., 1998 3416
beta-amyloid protein 40 P05067 672-711 peptide-Arg676+His-peptide P MS Ala Glu Phe Arg His Asp Ser Gly Tucker et al., 2000
beta-amyloid protein 40 P05067 672-711 peptide-Lys687+Leu-peptide P MS His His Gln Lys Leu Val Phe Phe Tucker et al., 2000
beta-amyloid protein 40 P05067 672-711 peptide-Lys699+Gly-peptide P MS Gly Ser Asn Lys Gly Ala Ile Ile Tucker et al., 2000
Boc-Glu-Lys-Lys-Mca Boc-Glu-Lys-Lys+Mca S Boc Glu Lys Lys Mca - - - Kato et al., 1980
Boc-Val-Leu-Lys-Mca Boc-Val-Leu-Lys+Mca S Boc Val Leu Lys Mca - - - Kato et al., 1980
brain-derived neurotrophic factor precursor P23560 19-247 peptide-Arg125+Val-peptide P Met Ser Met Arg Val Arg Arg His Gray & Ellis, 2008 22593
carboxypeptidase N P15169 21-458 peptide-Arg238+Arg-peptide P NT Arg Gly Val Arg Arg Thr Ala Ser Quagraine et al., 2005
carboxypeptidase N subunit 2 P22792 22-545 peptide-Arg478+Ser-peptide P NT Arg Ala Ala Arg Ser Gln Cys Thr Quagraine et al., 2005
ceruloplasmin P00450 20-1065 peptide-Arg500+Ser-peptide P NT Pro Gln Ser Arg Ser Val Pro Pro Kingston et al., 1978 3408
ceruloplasmin P00450 20-1065 peptide-Lys906+Val-peptide P NT Pro Tyr Leu Lys Val Phe Asn Pro Kingston et al., 1978 3407
chromogranin-A P10645 19-457 peptide-Arg377+Ala-peptide P Leu Ser Phe Arg Ala Arg Ala Tyr Jiang et al., 2001 18871
chromogranin-A P10645 19-457 peptide-Arg391+Arg-peptide P Pro Gln Leu Arg Arg Gly Trp Arg Jiang et al., 2001 18872
chromogranin-A P10645 19-457 peptide-Arg368+Asp-peptide P Glu Asp Asn Arg Asp Ser Ser Met Yuan et al., 2009 24727
chromogranin-A P10645 19-457 peptide-Lys373+Leu-peptide P Ser Ser Met Lys Leu Ser Phe Arg Yuan et al., 2009 24728
chromogranin-A P10645 19-457 peptide-Arg379+Ala-peptide P Phe Arg Ala Arg Ala Tyr Gly Phe Yuan et al., 2009 24729
chromogranin-A P10645 19-457 peptide-Arg384+Gly-peptide P Tyr Gly Phe Arg Gly Pro Gly Pro Yuan et al., 2009 24730
Coagulation factor VIII P00451 20-2351 Peptide-Lys55+Ser-Peptide P NT Arg Val Pro Lys Ser Phe Pro Phe Nogami et al., 2007
Coagulation factor VIII P00451 20-2351 Peptide-Arg355+Met-Peptide P NT Pro Gln Leu Arg Met Lys Asn Asn Nogami et al., 2007
Coagulation factor VIII P00451 20-2351 Peptide-Arg391+Ser-Peptide P NT Ile Gln Ile Arg Ser Val Ala Lys Nogami et al., 2007
Coagulation factor VIII P00451 20-2351 Peptide-Arg759+Ser-Peptide P NT Ile Glu Pro Arg Ser Phe Ser Gln Nogami et al., 2007
Coagulation factor VIII P00451 20-2351 Peptide-Arg1708+Ser-Peptide P NT Gln Ser Pro Arg Ser Phe Gln Lys Nogami et al., 2007
Coagulation factor VIII P00451 20-2351 Peptide-Arg1740+Ala-Peptide P NT Leu Arg Asn Arg Ala Gln Ser Gly Nogami et al., 2007
coagulation factor X light chain P00742 41-488 peptide-Arg335+Met-peptide P Ile Thr Phe Arg Met Asn Val Ala Pryzdial et al., 1999 24725
coagulation factor Xa P00742 41-179; 235-488 peptide-Lys370+Gly-peptide P Thr His Glu Lys Gly Arg Gln Ser Pryzdial et al., 1999 17428
Complement C3 alpha chain P01024 672-1663 peptide-Arg748+Ser-peptide P MS Gly Leu Ala Arg Ser Asn Leu Asp Amara et al.,2010
complement C5 P01031 678-1676 peptide-Arg751+Leu-peptide P MS Gln Leu Gly Arg Leu His Met Lys Amara et al., 2010
complement factor I P05156 19-335; 340-583 peptide-Lys344+Arg-peptide N Val Gly Gly Lys Arg Ala Gln Leu Tsiftsoglou et al., 2005
complement factor I P05156 19-335; 340-583 peptide-Lys319+Ser-peptide N Arg Arg Ile Lys Ser Leu Leu Pro Tsiftsoglou et al., 2005
complement factor I P05156 19-335; 340-583 peptide-Lys405+Arg-peptide N Pro Asp Leu Lys Arg Ile Val Ile Tsiftsoglou et al., 2005
complement factor I P05156 19-335; 340-583 peptide-Arg339+Ile-peptide N Arg Arg Lys Arg Ile Val Gly Gly Tsiftsoglou et al., 2005
complement factor I P05156 19-335; 340-583 peptide-Lys89+Ser-peptide N Cys Gln Gln Lys Ser Leu Glu Cys Tsiftsoglou et al., 2005
complement factor I P05156 19-335; 340-583 peptide-Lys37+Tyr-peptide N Leu Ala Lys Lys Tyr Thr His Leu Tsiftsoglou et al., 2005
complement factor I P05156 19-335; 340-583 peptide-Lys170+Leu-peptide N Arg Arg Phe Lys Leu Ser Asp Leu Tsiftsoglou et al., 2005
complement factor I P05156 19-335; 340-583 peptide-Arg79+Arg-peptide N Ala Thr Asn Arg Arg Ser Phe Pro Tsiftsoglou et al., 2005
complement factor I P05156 19-335; 340-583 peptide-Lys257+Ala-peptide N Leu Cys Cys Lys Ala Cys Gln Gly Tsiftsoglou et al., 2005
complement factor I P05156 19-335; 340-583 peptide-Lys36+Lys-peptide N Cys Leu Ala Lys Lys Tyr Thr His Tsiftsoglou et al., 2005
Elafin precursor P19957 23-117 peptide-Lys60+Ala-peptide P Asp Lys Val Lys Ala Gln Glu Pro Guyot et al., 2005 22162
fibrinogen alpha chain precursor P02671 19-866 peptide-Arg123+Asp-peptide P NT Ala Asn Asn Arg Asp Asn Thr Tyr Bini et al., 1999 23329
fibrinogen alpha chain precursor P02671 19-866 peptide-Arg35+Gly-peptide P Gly Gly Val Arg Gly Pro Arg Val Yuan et al., 2009 24714
fibrinogen alpha chain precursor P02671 19-866 peptide-Arg38+Val-peptide P Arg Gly Pro Arg Val Val Glu Arg Yuan et al., 2009 24715
fibrinogen beta chain P02675 31-491 peptide-Lys163+Asp-peptide P NT Lys Gln Val Lys Asp Asn Glu Asn Bini et al., 1999 23356
fibrinogen beta chain P02675 31-491 peptide-Lys83+Lys-peptide P Ala Thr Gln Lys Lys Val Glu Arg Yuan et al., 2009 24717
fibrinogen beta chain P02675 31-491 peptide-Arg72+Ala-peptide P Gly Gly Tyr Arg Ala Arg Pro Ala Yuan et al., 2009 24718
fibrinogen beta chain P02675 31-491 peptide-Lys77+Ala-peptide P Arg Pro Ala Lys Ala Ala Ala Thr Yuan et al., 2009 24719
fibrinogen beta chain P02675 31-491 peptide-Lys51+Lys-peptide P Pro Leu Asp Lys Lys Arg Glu Glu Yuan et al., 2009 24720
fibrinogen gamma chain P02679 27-453 peptide-Lys382+Ala-peptide P Thr Tyr Ser Lys Ala Ser Thr Pro Yuan et al., 2009 24721
fibrinogen gamma chain P02679 27-453 peptide-Lys399+Thr-peptide P Ala Thr Trp Lys Thr Arg Trp Tyr Yuan et al., 2009 24723
fibrinogen gamma chain P02679 27-453 peptide-Lys88+Ala-peptide P Gln Leu Ile Lys Ala Ile Gln Leu Yuan et al., 2009 23369
fibrinogen gamma chain P02679 27-453 peptide-Lys111+Ser-peptide P Ala Thr Leu Lys Ser Arg Lys Met Yuan et al., 2009 23366
Fibulin-2 P37889-1 27-1221 peptide-Lys263+Ala-peptide P NT Val Gln Ala Lys Ala Arg Arg Val Sasaki et al., 1996
Fibulin-2 P37889-1 27-1221 peptide-Arg406+Ala-peptide P NT Pro Arg Phe Arg Ala Glu Glu Asp Sasaki et al., 1996
Fibulin-2 P37889-1 27-1221 peptide-Arg565+Arg-peptide P NT Ala Ala Pro Arg Arg Val Ser Glu Sasaki et al., 1996
Fibulin-2 P37889-1 27-1221 peptide-Arg575+Glu-peptide P NT Met Ala Ser Arg Glu Ala Leu Ser Sasaki et al., 1996
Fibulin-2 P37889-1 27-1221 peptide-Arg652+Ser-peptide P NT Ser Ala Pro Arg Ser Glu Ser Ala Sasaki et al., 1996
fibulin-2 P37889 27-1221 peptide-Lys784+Ala-peptide P NT Gln Cys Tyr Lys Ala Leu Thr Cys Sasaki et al., 1996
glutamate [NMDA] receptor subunit epsilon-1 Q12879 23-1464 peptide-Lys317+Ala-peptide P NT Pro Glu Ala Lys Ala Ser Cys Tyr Yuan et al., 2009 24704
hemagglutinin precursor P03437 17-566 peptide-Arg345+Gly-peptide N Lys Gln Thr Arg Gly Leu Phe Gly Murakami et al., 2001
histidine-rich glycoprotein precursor Q28640 9-526 peptide-Arg303+His-peptide N Pro Arg Cys Arg His Arg Pro Phe Borza et al., 1996 17430
histidine-rich glycoprotein precursor Q28640 9-526 peptide-Lys421+Glu-peptide N Pro Ser His Lys Glu Gly Pro Gln Borza et al., 1996 17431
Hyaluronan and proteoglycan link protein 1 P10915 16-354 Peptide-Arg28+Ala-Peptide N NT Asp His Asp Arg Ala Ile His Ile Nguyen et al., 1991
IgG1 heavy chain (hinge region) P01857 1-330 peptide-Lys4+Thr-peptide P Ser Cys Asp Lys Thr His Thr Cys Ryan et al., 2008 20259
insulin-like growth factor binding protein 3 P17936 28-291 peptide-Arg122+Leu-peptide P Ala Val Ser Arg Leu Arg Ala Tyr Lalou et al., 1997 3418
insulin-like growth factor binding protein 3 P17936 28-291 peptide-Lys187+Val-peptide P Gln Arg Tyr Lys Val Asp Tyr Glu Lalou et al., 1997 3420
insulin-like growth factor binding protein 3 P17936 28-291 peptide-Lys225+Phe-peptide P Asn His Leu Lys Phe Leu Asn Val Lalou et al., 1997
insulin-like growth factor binding protein 3 P17936 28-291 peptide-Lys247+Lys-peptide P Gly Phe Tyr Lys Lys Lys Gln Cys Lalou et al., 1997
insulin-like growth factor binding protein 3 P17936 28-291 peptide-His169+Ser-peptide P His Pro Leu His Ser Lys Ile Ile Lalou et al., 1997
insulin-like growth factor binding protein 3 P17936 28-291 peptide-Arg185+Tyr-peptide P Asp Ser Gln Arg Tyr Lys Val Asp Lalou et al., 1997
insulin-like growth factor binding protein 3 P17936 28-291 peptide-Arg124+Ala-peptide P Ser Arg Leu Arg Ala Tyr Leu Leu Gibson & Cohen, 1999 3423
kallikrein-related peptidase 11 isoform 2 Q9UBX7 51-282 peptide-Arg188+Leu-peptide P Pro Gln Leu Arg Leu Pro His Thr Luo et al., 2006 20974
lysyl bond peptide-Lys+peptide N - - NPe Lys CPe - - - Castellino, 2004
matrix metallopeptidase-1 precursor P03956 20-469 peptide-Gln99+Phe100-peptide P Asp Val Ala Gln Phe Val Leu Thr Cawston, 2004 17432
matrix metallopeptidase-13 P45452 20-471 peptide-Lys57+Glu-peptide P Gly Ile Leu Lys Glu Asn Ala Ala Knauper et al., 1996 18319
matrix metallopeptidase-13 P45452 20-471 peptide-Arg95+Cys-peptide P Lys Lys Pro Arg Cys Gly Val Pro Knauper et al., 1996 17964
matrix metallopeptidase-13 P45452 20-471 peptide-Glu103+Tyr-peptide P Asp Val Gly Glu Tyr Asn Val Phe Knauper et al., 1996 17965
membrane-type matrix metallopeptidase-1 precursor P50281 21-582 peptide-Arg108+Arg-peptide P Ala Asn Val Arg Arg Lys Arg Tyr Okumura et al., 1997 18141
membrane-type matrix metallopeptidase-1 precursor P50281 21-582 peptide-Arg111+Tyr-peptide P Arg Arg Lys Arg Tyr Ala Ile Gln Okumura et al., 1997 18142
MEP1A Q8TDC9 1-746 peptide-Arg65+Asn-peptide P Gln Lys Ser Arg Asn Gly Leu Arg Rösmann et al., 2002 3415
neural cell adhesion molecule L1 P32004 20-1257 peptide-Lys860+His-peptide P Ser Gln Arg Lys His Ser Lys Arg Nayeem et al., 1999 19502
neural cell adhesion molecule L1 P32004 20-1257 peptide-Lys863+Arg-peptide P Lys His Ser Lys Arg His Ile His Nayeem et al., 1999 19503
nidogen-1 P10493 29-1245 peptide-Lys231+Ser-peptide P Phe Leu Trp Lys Ser Asn Gly Ala Walker et al., 1998 26821
nidogen-1 P10493 29-1245 peptide-Arg349+Ser-peptide P Glu Arg Thr Arg Ser Phe Gln Leu Walker et al., 1998 26822
osteocalcin P02818 52-100 peptide-Arg94+Arg-peptide P Glu Ala Tyr Arg Arg Phe Tyr Gly Yuan et al., 2009 24726
osteopontin P10451 17-314 peptide-Arg168+Ser-peptide P NT Tyr Gly Leu Arg Ser Lys Ser Lys Christensen et al., 2010
osteopontin P10451 17-314 peptide-Lys170+Ser-peptide P NT Leu Arg Ser Lys Ser Lys Lys Phe Christensen et al., 2010
osteopontin P10451 17-314 peptide-Asp178+Ile-peptide P NT Arg Arg Pro Asp Ile Gln Tyr Pro Christensen et al., 2010
osteopontin P10451 17-314 peptide-Lys203+Ala-peptide P NT Gly Ala Tyr Lys Ala Ile Pro Val Christensen et al., 2010
placenta growth factor isoform PIGF-4 P49763 19-221 peptide-Lys208+Met-peptide P NT Leu Arg Glu Lys Met Lys Pro Glu Kunnapuu et al., 2021
plasminogen P00747 20-810 peptide-Lys77+Lys-peptide P Leu Phe Glu Lys Lys Val Tyr Leu Castellino, 2004 17433
plasminogen P00747 20-810 peptide-Lys487+Gly-peptide P Gly Asn Gly Lys Gly Tyr Arg Gly Kwon et al., 2005 18916
proteinase-activated receptor 1 P25116 27-425 peptide-Lys32+Ala-peptide P Pro Glu Ser Lys Ala Thr Asn Ala Loew et al., 2000 24706
proteinase-activated receptor 1 P25116 27-425 peptide-Arg70+Leu-peptide P Thr Glu Tyr Arg Leu Val Ser Ile Loew et al., 2000 24708
proteinase-activated receptor 1 P25116 27-425 peptide-Lys76+Ser-peptide P Ser Ile Asn Lys Ser Ser Pro Leu Loew et al., 2000 24709
proteinase-activated receptor 1 P25116 27-425 peptide-Arg41+Ser-peptide P Leu Asp Pro Arg Ser Phe Leu Leu Loew et al., 2000 24707
proteinase-activated receptor 1 P25116 27-425 peptide-Lys82+Gln-peptide P Pro Leu Gln Lys Gln Leu Pro Ala Steinberg, 2004 24710
proteinase-activated receptor 2 P55085 26-397 peptide-Lys34+Gly-peptide P Arg Ser Ser Lys Gly Arg Ser Leu Loew et al., 2000 3427
proteinase-activated receptor 2 P55085 26-397 peptide-Arg36+Ser-peptide P Ser Lys Gly Arg Ser Leu Ile Gly Loew et al., 2000 3424
proteinase-activated receptor 4 precursor Q96RI0 18-385 peptide-Arg47+Gly-peptide P Pro Ala Pro Arg Gly Tyr Pro Gly Steinberg, 2004 20887
retinoic acid receptor responder protein 2 precursor Q99969 17-163 peptide-Lys158+Ala-peptide P Ala Phe Ser Lys Ala Leu Pro Arg Zabel et al., 2005
S-2238 D-Phe-Pip-Arg+NHPhNO2 S - DPh Pip Arg NAN - - -
S-2251 D-Val-Leu-Lys+NHPhNO2 S - DVa Leu Lys NAN - - -
S-2266 D-Val-Leu-Arg+NHPhNO2 S - DVa Leu Arg NAN - - -
S-2302 D-Pro-Phe-Arg+NHPhNO2 S - DPr Phe Arg NAN - - -
scnn1g sodium channel, nonvoltage-gated 1 gamma Q9WU39 1-655 peptide-Lys194+Ala-peptide P Ile Ile His Lys Ala Ser Asn Val Passero et al., 2008 25557
small-inducible cytokine A14 precursor Q16627 20-93 peptide-Arg27+Gly-peptide P Ser Ser Ser Arg Gly Pro Tyr His Vakili et al., 2001
small-inducible cytokine A2 precursor P10148 24-148 peptide-Lys104+Thr-peptide P Thr Leu Phe Lys Thr Ala Ser Ala Sheehan et al., 2007
syndecan-1 P18827 23-310 peptide-Arg230+Asn-peptide P NT Pro Asp Arg Arg Asn Gln Ser Pro Manon-Jensen et al., 2013
Syndecan-4 P31431 19-198 peptide-Lys114+Arg-peptide P Val Ile Pro Lys Arg Ile Ser Pro Schmidt et al., 2005 18868
Syndecan-4 P31431 19-198 peptide-Lys128+Val-peptide P Val Ser Asn Lys Val Ser Met Ser Schmidt et al., 2005 18870
tissue-type plasminogen activator P00750 36-562 peptide-Arg275+Ile-peptide P Pro Gln Phe Arg Ile Lys Gly Gly Tate et al., 1987 18843
Tos-Gly-Pro-Lys-NHPhNO2 Tos-Gly-Pro-Lys+NHPhNO2 S Tos Gly Pro Lys NAN - - -
urokinase plasminogen activator surface receptor Q03405 23-305 peptide-Arg111+Ser-peptide P Thr Tyr Ser Arg Ser Arg Tyr Leu Andolfo et al., 2002 19562
urokinase plasminogen activator surface receptor Q03405 23-305 peptide-Arg105+Ala-peptide P Asn Ser Gly Arg Ala Val Thr Tyr Andolfo et al., 2002 19939
urokinase plasminogen activator surface receptor P35456 24-298 peptide-Arg109+Ala-peptide P Ala Arg Gly Arg Ala Phe Pro Gln Andolfo et al., 2002 19937
urokinase plasminogen activator surface receptor P35456 24-298 peptide-Arg107+Gly-peptide P Pro Gly Ala Arg Gly Arg Ala Phe Andolfo et al., 2002
urokinase-type plasminogen activator P00749 21-431 peptide-Lys158+Ile-peptide P Pro Arg Phe Lys Ile Ile Gly Gly Ellis, 2004 17436
urokinase-type plasminogen activator P00749 21-431 peptide-Lys135+Lys-peptide P Ala Asp Gly Lys Lys Pro Ser Ser Ellis, 2004 17435
vascular endothelial growth factor A P15692 27-232 peptide-Arg136+Ala-peptide P NT Lys Lys Asp Arg Ala Arg Gln Glu Kunnapuu et al., 2021
vascular endothelial growth factor C P49767 32-111 peptide-Arg102+Thr-peptide P NT Leu Asn Ser Arg Thr Glu Glu Thr Kunnapuu et al., 2021
vascular endothelial growth factor C P49767 32-111 peptide-Arg127+ P NT Asn Glu Trp Arg '-' '-' '-' '-' Kunnapuu et al., 2021
vascular endothelial growth factor D O43915 22-354 peptide-Arg108+Thr-peptide P NT Glu Trp Gln Arg Thr Gln Cys Ser Kunnapuu et al., 2021
vascular endothelial growth factor D precursor O43915 22-354 peptide-Arg88+Phe-peptide P Arg Ser Thr Arg Phe Ala Ala Thr McColl et al., 2003 23304
vascular endothelial growth factor D precursor O43915 22-354 peptide-Lys100+Val-peptide P Glu Thr Leu Lys Val Ile Asp Glu McColl et al., 2003 23305
vascular endothelial growth factor D precursor O43915 22-354 peptide-Arg205+Ser-peptide P Ile Ile Arg Arg Ser Ile Gln Ile McColl et al., 2003 23306
vitronectin P04004 20-478 peptide-Arg361+Ser-peptide P Lys Gly Tyr Arg Ser Gln Arg Gly Chain et al., 1991 17437

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