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Substrates for peptidase M10.001: matrix metallopeptidase-1

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Pharma

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate Uniprot Residue range Cleavage Site Cleavage type Evidence P4 P3 P2 P1 P1' P2' P3' P4' Reference CutDB MERNUM
aggrecan core protein P16112 17-2415 peptide-Asn360+Phe-peptide P Ile Pro Glu Asn Phe Phe Gly Val Fosang et al., 1996 3208
aggrecan core protein P16112 17-2415 peptide-Asp460+Leu-peptide P Thr Ser Glu Asp Leu Val Val Gln Fosang et al., 1996 3205
Aggrecan core protein Q29011 1-537 peptide-Asn344+Phe-peptide P NT Ile Pro Glu Asn Phe Phe Gly Val Fosang et al., 1993
Aggrecan core protein Q29011 1-537 peptide-Asp447+Leu-peptide P NT Thr Ser Glu Asp Leu Val Val Gln Fosang et al., 1993
Ala-Leu-Ala-Leu-Arg-Val-Thr Ala-Leu-Ala+Leu-Arg-Val-Thr N - Ala Leu Ala Leu Arg Val Thr Cawston, 2004
alpha-1-antichymotrypsin P01011 24-423 peptide-Ala362+Leu-peptide P Leu Leu Ser Ala Leu Val Glu Thr Mast et al., 1991 18459
Alpha-1-inhibitor 3 P14046 25-1477 peptide-Pro707+Val-peptide P NT Glu Ser Leu Pro Val Val Ala Val <%Sottrup-Jensen & Birkedal-Hansen, 1989[20040511G011]%
Alpha-1-macroglobulin Q63041 25-1500 peptide-Ala706+Leu-peptide P NT Glu Pro Gln Ala Leu Ala Met Ser <%Sottrup-Jensen & Birkedal-Hansen, 1989[20040511G011]%
Alpha-1-macroglobulin Q63041 25-1500 peptide-Ala708+Met-peptide P NT Gln Ala Leu Ala Met Ser Ala Ile <%Sottrup-Jensen & Birkedal-Hansen, 1989[20040511G011]%
alpha-1-peptidase inhibitor P01009 25-418 peptide-Phe352+Leu-peptide P Gly Ala Met Phe Leu Glu Ala Ile Nelson et al., 1998 19084
alpha-1-peptidase inhibitor P01009 25-418 peptide-Pro357+Met-peptide P Glu Ala Ile Pro Met Ser Ile Pro Desrochers et al., 1991 16535
alpha-2-macroglobulin P01023 24-1474 peptide-Gly679+Leu-peptide P Gly Pro Glu Gly Leu Arg Val Gly Cawston, 2004 16536
Alpha-2-macroglobulin P06238 28-1472 peptide-His708+Leu-peptide P NT Ala Ala Tyr His Leu Val Ser Gln <%Sottrup-Jensen & Birkedal-Hansen, 1989[20040511G011]%
Alpha-2-macroglobulin P06238 28-1472 peptide-Phe718+Leu-peptide P NT Met Asp Ala Phe Leu Glu Ser Ser <%Sottrup-Jensen & Birkedal-Hansen, 1989[20040511G011]%
alpha-synuclein P37840 1-140 peptide-Lys21+Thr-peptide P Ala Ala Glu Lys Thr Lys Gln Gly Levin etal, 2008
alpha-synuclein P37840 1-140 peptide-Gly41+Ser-peptide P Leu Tyr Val Gly Ser Lys Thr Lys Levin etal, 2008
alpha-synuclein P37840 1-140 peptide-Gly47+Val-peptide P Thr Lys Glu Gly Val Val His Gly Levin etal, 2008
alpha-synuclein P37840 1-140 peptide-Lys97+Asp-peptide P Phe Val Lys Lys Asp Gln Leu Gly Levin etal, 2008
alpha-synuclein P37840 1-140 peptide-Ala90+Ala-peptide P Ser Ile Ala Ala Ala Thr Gly Phe Levin etal, 2008
alpha-synuclein P37840 1-140 peptide-Ala78+Gln-peptide P Thr Ala Val Ala Gln Lys Thr Val Levin etal, 2008
alpha-synuclein P37840 1-140 peptide-Val71+Thr-peptide P Gly Ala Val Val Thr Gly Val Thr Levin etal, 2008
alpha-synuclein P37840 1-140 peptide-Val70+Val-peptide P Gly Gly Ala Val Val Thr Gly Val Levin etal, 2008
alpha-synuclein P37840 1-140 peptide-Ala18+Ala-peptide P Val Val Ala Ala Ala Glu Lys Thr Levin etal, 2008
alpha-synuclein P37840 1-140 peptide-Tyr133+Gln-peptide P Glu Glu Gly Tyr Gln Asp Tyr Glu Levin etal, 2008
alpha-synuclein P37840 1-140 peptide-Gly7+Leu-peptide P Phe Met Lys Gly Leu Ser Lys Ala Levin etal, 2008
alpha-synuclein P37840 1-140 peptide-Gln134+Asp-peptide P Glu Gly Tyr Gln Asp Tyr Glu Pro Levin etal, 2008
brevican core protein precursor P55068 23-833 peptide-Ala360+Phe-peptide P His Pro Ser Ala Phe Ser Glu Ala Nakamura et al., 2001 18213
C-C motif chemokine 2 P13500 24-99 peptide-Ala27+Ile-peptide P Gln Pro Asp Ala Ile Asn Ala Pro McQuibban et al., 2002 18378
C-C motif chemokine 7 P80098 24-99 peptide-Gly27+Ile-peptide P Gln Pro Val Gly Ile Asn Thr Ser McQuibban et al., 2002 18380
C-X-C motif chemokine 11 precursor Q9JHH5 22-100 peptide-Leu84+Ile-peptide P Gln Ala Arg Leu Ile Met Gln Ala Cox et al., 2008
C-X-C motif chemokine 11 precursor Q9JHH5 22-100 peptide-Phe94+Leu-peptide P Lys Lys Asn Phe Leu Arg Arg Gln Cox et al., 2008
C-X-C motif chemokine 5 P42830 37-114 peptide-Arg45+Glu-peptide P Ala Val Leu Arg Glu Leu Arg Cys Tester et al., 2007
C-X-C motif chemokine 5 P50228 41-132 Ala-Pro-Ser-Ser44+Val-peptide P NT Ala Pro Ser Ser Val Ile Ala Ala Tester et al., 2007
collagen alpha 1(I) chain P02453 162-1215 peptide-Gly775+Ile-peptide P Gly Pro Gln Gly Ile Ala Gly Gln Aimes & Quigley, 1995
collagen alpha 2(I) chain P02465 80-1100 peptide-Gly775+Leu-peptide P Gly Pro Gln Gly Leu Leu Gly Ala Aimes & Quigley, 1995
collagen alpha 2(I) chain P08123 80-1102 peptide-Gly775+Leu-peptide P Gly Pro Gln Gly Leu Leu Gly Ala Billinghurst et al., 1997
collagen alpha-1(I) chain P02452 162-1218 peptide-Gly775+Ile-peptide P Gly Pro Gln Gly Ile Ala Gly Gln Cawston, 2004
collagen alpha-1(II) chain P02458 182-1241 peptide-Gly906-Leu-peptide P Gly Pro Gln Gly Leu Ala Gly Gln
collagen alpha-1(II) chain P02458 182-1241 peptide-Gly906+Pro-peptide P Gly Arg Val Gly Pro Pro Gly Ser Mitchell et al., 1996 3214
collagen alpha-1(II) chain P02458 182-1241 peptide-Gly975+Leu-peptide P Gly Pro Gln Gly Leu Ala Gly Gln Billinghurst et al., 1997
collagen alpha-1(III) chain P02461 24-1466 peptide-Gly948+Ile-peptide P Gly Pro Leu Gly Ile Ala Gly Ile Billinghurst et al., 1997
complement C1q subcomponent subunit A P02745 23-245 peptide-Asn108+Ile-peptide N Ser Pro Gly Asn Ile Lys Asp Gln Ruiz et al., 1999 19774
connective tissue growth factor P29279 27-349 peptide-Met194+Ile-peptide P Asp Pro Thr Met Ile Arg Ala Asn Hashimoto et al., 2002 19450
connective tissue growth factor P29279 27-349 peptide-Cys199+Leu-peptide P Arg Ala Asn Cys Leu Val Gln Thr Hashimoto et al., 2002 19456
Dabcyl-Leu-Ala-Gln-Ala-HPh-Arg-Ser-Lys(Fam)-NH2 Dabcyl-Leu-Ala-Gln-Ala+HPh-Arg-Ser-Lys(Fam)-NH2 S Leu Ala Gln Ala HPh Arg Ser LyN Moss & Rasmussen, 2007
Dabcyl-Pro-Arg-Ala-Ala-Ala-hPhe-Thr-Ser-Pro-Lys(FAM)-NH2 Dabcyl-Pro-Arg-Ala-Ala-Ala+homoPhe-Thr-Ser-Pro-Lys(FAM)-NH2 S Arg Ala Ala Ala HPh Thr Ser Pro Moss et al., 2016
Dabcyl-Pro-Cha-Gly-Cys(Me)-His-Ala-Lys(Fam)-NH2 Dabcyl-Pro-Cha-Gly+Cys(Me)-His-Ala-Lys(Fam)-NH2 S Dab Pro Cha Gly CyM His Ala LyN Moss & Rasmussen, 2007
Dabcyl-Ser-Pro-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Lys(FAM)-NH2 Dabcyl-Ser-Pro-Leu-Ala-Gln-Ala+Val-Arg-Ser-Ser-Lys(FAM)-NH2 S Leu Ala Gln Ala Val Arg Ser Ser Moss et al., 2016
Dnp-Pro-Leu-Ala-Leu-Trp-Ala-Arg Dnp-Pro-Leu-Ala+Leu-Trp-Ala-Arg S Dnp Pro Leu Ala Leu Trp Ala Arg Cawston, 2004
Glu(EDANS)-Pro-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser(TF)-Ser-Lys(DABCYL) Glu(EDANS)-Pro-Leu-Ala-Gln-Ala+Val-Arg-Ser-Ser(TF)-Ser-Lys(DABCYL) S Leu Ala Gln Ala Val Arg Ser Ser Minond et al., 2012
Glu(EDANS)-Pro-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser(TF)-Ser-Lys(DABCYL) Glu(EDANS)-Pro-Leu-Ala-Gln+Ala-Val-Arg-Ser-Ser(TF)-Ser-Lys(DABCYL) S Pro Leu Ala Gln Ala Val Arg Ser Minond et al., 2012
Glu(EDANS)-Pro-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Lys(DABCYL) Glu(EDANS)-Pro-Leu-Ala-Gln-Ala+Val-Arg-Ser-Ser(TF)-Ser-Lys(DABCYL) S Leu Ala Gln Ala Val Arg Ser Ser Minond et al., 2012
Glu(EDANS)-Pro-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Lys(DABCYL) Glu(EDANS)-Pro-Leu-Ala+Gln-Ala-Val-Arg-Ser-Ser(TF)-Ser-Lys(DABCYL) S Glu Pro Leu Ala Gln Ala Val Arg Minond et al., 2012
Glu(EDANS)-Pro-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Lys(DABCYL) Glu(EDANS)-Pro-Leu-Ala-Gln-Ala-Val-Arg+Ser-Ser(TF)-Ser-Lys(DABCYL) S Gln Ala Val Arg Ser Ser Ser Lys Minond et al., 2012
hyaluronan and proteoglycan link protein 1 P10915 16-354 peptide-His31+Ile-peptide P Arg Ala Ile His Ile Gln Ala Glu Nguyen et al., 1993
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr+Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N MS Glu Ala Leu Tyr Leu Val Cya Gly Roghani et al., 1999
insulin-like growth factor binding protein 3 P17936 28-291 peptide-Tyr126+Leu-peptide P Leu Arg Ala Tyr Leu Leu Pro Ala Fowlkes et al., 1994 3209
insulin-like growth factor binding protein 3 P17936 28-291 peptide-Arg124+Ala-peptide P Ser Arg Leu Arg Ala Tyr Leu Leu Gibson & Cohen, 1999
insulin-like growth factor binding protein 3 P17936 28-291 peptide-His169+Ser-peptide N His Pro Leu His Ser Lys Ile Ile Gibson & Cohen, 1999
insulin-like growth factor binding protein 3 P17936 28-291 peptide-Leu123+Arg-peptide P Val Ser Arg Leu Arg Ala Tyr Leu Fowlkes et al., 1994 3211
insulin-like growth factor binding protein 3 P17936 28-291 peptide-Leu168+His-peptide P Phe His Pro Leu His Ser Lys Ile Fowlkes et al., 1994 3210
interleukin-8 P10145 23-99 peptide-Ser28+Ala-peptide P Leu Pro Arg Ser Ala Lys Glu Leu Tester et al., 2007 21345
LPS-induced CXC chemokine Q8K3I3 41-132 peptide-Ser44+Val-peptide P Ala Pro Ser Ser Val Ile Ala Ala Tester et al., 2007 21341
matrix metallopeptidase-1 C9VZX3 100-469 peptide-Ala238+Ile-peptide P Gly Ile Gln Ala Ile Tyr Gly Pro Cawston, 2004 16539
matrix metallopeptidase-1 precursor P03956 20-469 peptide-Val86+Met-peptide P Thr Leu Lys Val Met Lys Gln Pro Suzuki et al., 1991 18156
matrix metallopeptidase-1 precursor P03956 20-469 peptide-Phe100+Val-peptide P Val Ala Gln Phe Val Leu Thr Glu Desrochers et al., 1991 18157
matrix metallopeptidase-1 precursor P03956 20-469 peptide-Val101+Leu-peptide P Ala Gln Phe Val Leu Thr Glu Gly Desrochers et al., 1991 18158
matrix metallopeptidase-1 precursor P03956 20-469 peptide-Thr83+Leu-peptide P Asp Ala Glu Thr Leu Lys Val Met Desrochers et al., 1991 18160
matrix metallopeptidase-1 precursor P03956 20-469 peptide-Gln99+Phe-peptide P Asp Val Ala Gln Phe Val Leu Thr Desrochers et al., 1991 18208
matrix metallopeptidase-1 precursor P03956 100-469 peptide-Pro269+Ile-peptide P Pro Val Gln Pro Ile Gly Pro Gln Desrochers et al., 1991 16538
matrix metallopeptidase-9 P14780 20-707 peptide-Glu59+Met-peptide P Arg Val Ala Glu Met Arg Gly Glu Sang et al., 1995
matrix metallopeptidase-9 P14780 20-707 peptide-Ala93+Met-peptide P Thr Leu Lys Ala Met Arg Thr Pro Sang et al., 1995
matrix metallopeptidase-9 P14780 20-707 peptide-Arg106+Phe-peptide P Asp Leu Gly Arg Phe Gln Thr Phe Sang et al., 1995
Mca-Pro-Cha-Gly-Nva-His-Ala-Dpa-NH2 Mca-Pro-Cha-Gly+Nva-His-Ala-Dpa-NH2 S Mca Pro Cha Gly nVa His Ala Dpa
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 Mca-Pro-Leu-Gly+Leu-Dpa-Ala-Arg-NH2 S Mca Pro Leu Gly Leu Dpa Ala Arg Knight et al., 1992
mini-agrin Q5EBX5 1-961 peptide-Asn147+Leu-peptide P NT Thr Leu Arg Asn Leu Glu Glu Val Patel et al., 2021
Pregnancy zone protein P20742 26-1482 peptide-Gly704+Leu-peptide P NT Tyr Gly Ala Gly Leu Gly Val Val <%Sottrup-Jensen & Birkedal-Hansen, 1989[20040511G011]%
Pregnancy zone protein P20742 26-1482 peptide-Gly706+Val-peptide P NT Ala Gly Leu Gly Val Val Glu Arg <%Sottrup-Jensen & Birkedal-Hansen, 1989[20040511G011]%
Pregnancy zone protein P20742 26-1482 peptide-Gly785+Ile-peptide P NT Ala Gly Leu Gly Ile Ser Ser Thr <%Sottrup-Jensen & Birkedal-Hansen, 1989[20040511G011]%
prolactin P01236 29-227 peptide-Lys81+Ala-peptide P Phe Ile Thr Lys Ala Ile Asn Ser Macotela et al., 2006 21567
prolactin P01236 29-227 peptide-Leu184+Gln-peptide P Leu Pro Ser Leu Gln Met Ala Asp Macotela et al., 2006
prolactin P01236 29-227 peptide-Ala139+Ile-peptide P Ala Pro Glu Ala Ile Leu Ser Lys Macotela et al., 2006
proteinase-activated receptor 1 P25116 27-425 peptide-Arg44+Ser-peptide P Leu Asp Pro Arg Ser Phe Leu Leu Boire et al., 2005 19518
QF-24 Mca-Pro-Leu-Gly+Leu-Dpa-Ala-Arg-NH2 S Mca Pro Leu Gly Leu Dpa Ala Arg Cawston, 2004
Serum amyloid A protein P0DJI8 19-122 peptide-Asp41+Met-peptide P MS Ala Tyr Ser Asp Met Arg Glu Ala van der Hilst et al., 2008
Serum amyloid A protein P0DJI8 19-122 peptide-Tyr47+Ile-peptide P MS Glu Ala Asn Tyr Ile Gly Ser Asp van der Hilst et al., 2008
serum amyloid A-2 protein P0DJI9 19-122 peptide-Val75+Ile-peptide P MS Ala Ala Glu Val Ile Ser Asn Ala Stix et al., 2001 3212
small inducible cytokine A13 Q99616 17-98 peptide-Asp26+Ala-peptide P Ala Gln Pro Asp Ala Leu Asn Val McQuibban et al., 2002 18384
small inducible cytokine A13 Q99616 17-98 peptide-Val30+Pro-peptide P Ala Leu Asn Val Pro Ser Thr Cys McQuibban et al., 2002 18385
small inducible cytokine A13 Q99616 17-98 peptide-Ala27+Leu-peptide P Gln Pro Asp Ala Leu Asn Val Pro McQuibban et al., 2002
tissue factor pathway inhibitor P10646 29-304 peptide-Lys48+Leu-peptide P Pro Pro Leu Lys Leu Met His Ser Belaaouaj et al., 2000 18115

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