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Substrates for peptidase S01.251: kallikrein-related peptidase 4

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Pharma

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate Uniprot Residue range Cleavage Site Cleavage type Evidence P4 P3 P2 P1 P1' P2' P3' P4' Reference CutDB MERNUM
Abz-Gly-Arg-Gly-Gly-Pro-Leu-Ala-Tyr-Gly-Ala-Met-Phe-Pro-Gly-Tyr(NO2) Abz-Gly-Arg+Gly-Gly-Pro-Leu-Ala-Tyr-Gly-Ala-Met-Phe-Pro-Gly-Tyr(NO2) S - Abz Gly Arg Gly Gly Pro Leu Chun et al., 2010
Abz-Gly-Arg-Gly-Gly-Pro-Leu-Ala-Tyr-Gly-Ala-Met-Phe-Pro-Gly-Tyr(NO2) Abz-Gly-Arg-Gly-Gly-Pro-Leu-Ala-Tyr-Gly+Ala-Met-Phe-Pro-Gly-Tyr(NO2) S Leu Ala Tyr Gly Ala Met Phe Pro Chun et al., 2010
Abz-Gly-Arg-Gly-Gly-Pro-Leu-Ala-Tyr-Gly-Ala-Met-Phe-Pro-Gly-Tyr(NO2) Abz-Gly-Arg-Gly-Gly-Pro-Leu-Ala-Tyr+Gly-Ala-Met-Phe-Pro-Gly-Tyr(NO2) S Pro Leu Ala Tyr Gly Ala Met Phe Chun et al., 2010
Abz-Gly-Leu-Ala-Asp-Ala-Tyr-Glu-Thr-Tyr-Gly-Ala-Tyr(NO2) Abz-Gly-Leu-Ala-Asp-Ala-Tyr-Glu-Thr-Tyr+Gly-Ala-Tyr(NO2) S Tyr Glu Thr Tyr Gly Ala TyN - Chun et al., 2010
Abz-Ser-Leu-Gln-Gly-Leu-Asn-Met-Leu-Ser-Gln-Tyr-Ser-Lys(Dnp) Abz-Ser-Leu+Gln-Gly-Leu-Asn-Met-Leu-Ser-Gln-Tyr-Ser-Lys(Dnp) S - Abz Ser Leu Gln Gly Leu Asn Chun et al., 2010
Abz-Ser-Met-Phe-Pro-Ile-Ala-Arg-Leu-Ile-Ser-Gln-Gly-Pro-Val-Tyr(NO2) Abz-Ser-Met-Phe-Pro-Ile-Ala-Arg+Leu-Ile-Ser-Gln-Gly-Pro-Val-Tyr(NO2) S Pro Ile Ala Arg Leu Ile Ser Gln Chun et al., 2010
ameloblastin Q28989 27-421 peptide-Gln54+Gly-peptide P NT Gly Ser Leu Gln Gly Leu Asn Met Chun et al., 2010
ameloblastin Q28989 27-421 peptide-Lys69+Ser-peptide P NT Gly Phe Gly Lys Ser Phe Asn Ser Chun et al., 2010
ameloblastin Q28989 27-421 peptide-Arg196+Leu-peptide P NT Pro Ile Ala Arg Leu Ile Ser Gln Chun et al., 2010
ameloblastin Q28989 27-421 peptide-Tyr217+Met-peptide P NT Gly Met Phe Tyr Met Ser Tyr Gly Chun et al., 2010
ameloblastin Q28989 27-421 peptide-Arg417+Phe-peptide P NT Asp Ala Trp Arg Phe Gln Glu Pro Chun et al., 2010
Amelogenin P45561-1 73-86 peptide-His78+Ala-peptide S MS Pro Gln Ser His Ala Leu Gln Pro Nagano et al., 2009
Amelogenin P45561-1 158-171 peptide-Gln166+Ser-peptide S MS Phe Ser Met Gln Ser Leu Leu Pro Nagano et al., 2009
Amelogenin P45561-1 17-49,169-189 peptide-Asn30+Phe-peptide S MS Gly Tyr Ile Asn Phe Ser Tyr Glu Nagano et al., 2009
Amelogenin P45561-1 17-49,169-189 peptide-Phe31+Ser-peptide S MS Tyr Ile Asn Phe Ser Tyr Glu Val Nagano et al., 2009
Amelogenin P45561-1 17-49,169-189 peptide-Lys40+Trp-peptide S MS Thr Pro Leu Lys Trp Tyr Gln Asn Nagano et al., 2009
Amelogenin P45561-1 17-49,169-189 peptide-Tyr42+Gln-peptide S MS Leu Lys Trp Tyr Gln Asn Met Ile Nagano et al., 2009
Amelogenin P45561-1 17-49,169-189 peptide-Asn44+Met-peptide S MS Trp Tyr Gln Asn Met Ile Arg His Nagano et al., 2009
Amelogenin P45561-1 17-49,169-189 peptide-Arg47+His-peptide S MS Asn Met Ile Arg His Pro Ser Leu Nagano et al., 2009
Amelogenin P45561 17-189 Peptide-Leu39+Lys-Peptide P - Leu Thr Pro Leu Lys Trp Tyr Gln Debela et al., 2008
Amelogenin P45561 17-189 Peptide-Ala124+Gln-Peptide P - Pro Leu Pro Ala Gln Gln Pro Phe Debela et al., 2008
Amelogenin P45561 17-189 Peptide-Tyr53+Gly-Peptide P NT Tyr Thr Ser Tyr Gly Tyr Glu Pro Ryu et al., 2002
Amelogenin P45561 17-189 Peptide-His78+Ala-Peptide P NT Pro Gln Ser His Ala Leu Gln Pro Ryu et al., 2002
Amelogenin P45561 17-189 Met17+Pro-Peptide P NT - - - Met Pro Leu Pro Pro Ryu et al., 2002
Amelogenin P45561 17-189 Peptide-Arg47+His-Peptide P NT Asn Met Ile Arg His Pro Tyr Thr Ryu et al., 2002
Amelogenin P45561 17-189 Peptide-Leu39+Lys-Peptide P NT Leu Thr Pro Leu Lys Trp Tyr Gln Ryu et al., 2002
Apolipoprotein F precursor Q13790 1-326 peptide-Arg164+Ser-peptide P CS Arg Val Gly Arg Ser Leu Pro Thr Matsumura et al., 2005
Atrial natriuretic peptide receptor 3 precursor P17342 1-541 peptide-Arg45+Glu-peptide P CS Arg Gln Glu Arg Glu Ala Leu Pro Matsumura et al., 2005
Bone morphogenetic protein 5 precursor P22003 1-454 peptide-Arg316+Ala-peptide P CS Arg Ser Val Arg Ala Ala Asn Lys Matsumura et al., 2005
Bone morphogenetic protein 6 precursor P22004 1-513 peptide-Arg374+Ser-peptide P CS Arg Thr Thr Arg Ser Ala Ser Ser Matsumura et al., 2005
Bone morphogenetic protein 7 precursor P18075 1-431 peptide-Arg299+Ser-peptide P CS Ser Lys Gln Arg Ser Gln Asn Arg Matsumura et al., 2005
Brain-derived neurotrophic factor precursor P23560 1-247 peptide-Arg128+His-peptide P CS Arg Val Arg Arg His Ser Asp Pro Matsumura et al., 2005
Cadherin-1 precursor P12830 1-882 peptide-Arg154+Asp-peptide P CS Arg Gln Lys Arg Asp Trp Val Ile Matsumura et al., 2005
Cadherin-13 precursor P55290 1-693 peptide-Arg138+Ser-peptide P CS Arg Gln Lys Arg Ser Ile Val Val Matsumura et al., 2005
Cadherin-2 precursor P19022 1-906 peptide-Arg159+Asp-peptide P CS Arg Gln Lys Arg Asp Trp Val Ile Matsumura et al., 2005
Cadherin-3 precursor P22223 1-829 peptide-Arg107+Asp-peptide P CS Arg His Lys Arg Asp Trp Val Val Matsumura et al., 2005
Cadherin-4 precursor P55283 1-916 peptide-Arg166+Arg-peptide P CS Gly Leu Arg Arg Arg Lys Arg Asp Matsumura et al., 2005
Cadherin-5 precursor P33151 1-784 peptide-Arg47+Asp-peptide P CS Arg Gln Lys Arg Asp Trp Ile Trp Matsumura et al., 2005
Calcitonin precursor P01258 1-116 peptide-Arg84+Cys-peptide P CS Arg Ser Lys Arg Cys Gly Asn Leu Matsumura et al., 2005
Carboxypeptidase A1 precursor P15085 1-419 peptide-Arg110+Ala-peptide P CS Phe Arg Ser Arg Ala Arg Ser Thr Matsumura et al., 2005
Carboxypeptidase A2 precursor P48052 1-419 peptide-Arg112+Glu-peptide P CS Asn Arg Arg Arg Glu Arg Ser Gly Matsumura et al., 2005
Carboxypeptidase B P15086 1-417 peptide-Arg110+Ala-peptide P CS Ser Arg Val Arg Ala Thr Gly His Matsumura et al., 2005
Cathepsin K precursor P43235 1-329 peptide-Arg114+Ala-peptide P CS Trp Glu Gly Arg Ala Pro Asp Ser Matsumura et al., 2005
Chymotrypsin-C precursor Q99895 1-268 peptide-Arg29+Val-peptide P CS Leu Ser Ala Arg Val Val Gly Gly Matsumura et al., 2005
Chymotrypsin-like elastase family member 2A precursor P08217 1-269 peptide-Arg28+Val-peptide P CS Tyr Val Thr Arg Val Val Gly Gly Matsumura et al., 2005
Chymotrypsin-like elastase family member 2B precursor P08218 1-269 peptide-Arg28+Met-peptide P CS Asp Met Ser Arg Met Leu Gly Gly Matsumura et al., 2005
Chymotrypsin-like elastase family member 3A precursor P09093 1-270 peptide-Arg28+Val-peptide P CS Ser Ser Ser Arg Val Val His Gly Matsumura et al., 2005
Chymotrypsin-like elastase family member 3B precursor P08861 1-270 peptide-Arg28+Val-peptide P CS Pro Ser Ser Arg Val Val Asn Gly Matsumura et al., 2005
Coagulation factor IX precursor P00740 1-461 peptide-Arg226+Val-peptide P CS Asp Phe Thr Arg Val Val Gly Gly Matsumura et al., 2005
Coagulation factor X precursor P00742 1-488 peptide-Arg234+Ile-peptide P CS Asn Leu Thr Arg Ile Val Gly Gly Matsumura et al., 2005
Coagulation factor XIII A chain P00488 2-732 peptide-Arg38+Gly-peptide P CS Val Val Pro Arg Gly Val Asn Leu Matsumura et al., 2005
Complement component C8 alpha chain precursor P07357 1-584 peptide-Arg30+Ala-peptide P CS Arg Val Arg Arg Ala Ala Thr Pro Matsumura et al., 2005
Complement component C8 beta chain precursor P07358 1-591 peptide-Arg54+Ser-peptide P CS Arg Gln Met Arg Ser Val Asp Val Matsumura et al., 2005
Defensin-5 precursor Q01523 1-94 peptide-Arg62+Ala-peptide P CS Ser Gln Ala Arg Ala Thr Cys Tyr Matsumura et al., 2005
Desmocollin-2 precursor Q02487-2 1-847 peptide-Arg135+Arg-peptide P CS Arg Ala Lys Arg Arg Trp Ala Pro Matsumura et al., 2005
Desmoglein-1 precursor Q02413 1-1049 peptide-Arg49+Glu-peptide P CS Arg Gln Lys Arg Glu Trp Ile Lys Matsumura et al., 2005
Desmoglein-3 precursor P32926 1-999 peptide-Arg49+Glu-peptide P CS Arg Gln Lys Arg Glu Trp Val Lys Matsumura et al., 2005
enamelin O97939 39-1142 peptide-Arg180+Ile-peptide P Val Pro His Arg Ile Pro Pro Gly Yamakoshi et al., 2006 20120
enamelin O97939 39-1142 peptide-Tyr205+His-peptide P Phe Phe Gly Tyr His Gly Phe Gly Yamakoshi et al., 2006 20121
enamelin O97939 39-1142 peptide-Met219+Phe-peptide P Ser Glu Glu Met Phe Glu Gln Asp Yamakoshi et al., 2006 20122
enamelin O97939 39-1142 peptide-Arg262+Gly-peptide P Pro Asn Pro Arg Gly Asn Asp Thr Yamakoshi et al., 2006 20123
enamelin O97939 39-1142 peptide-His206+Gly-peptide P Phe Gly Tyr His Gly Phe Gly Gly Yamakoshi et al., 2006
Epithelial cell adhesion molecule precursor P16422 1-314 peptide-Arg81+Ala-peptide P CS Leu Gly Arg Arg Ala Lys Pro Glu Matsumura et al., 2005
Fibronectin precursor P02751 1-86 peptide-Arg31+Gln-peptide P CS Lys Ser Lys Arg Gln Ala Gln Gln Matsumura et al., 2005
Glial cell line-derived neurotrophic factor precursor P39905 1-211 peptide-Arg77+Ser-peptide P CS Arg Leu Lys Arg Ser Pro Asp Lys Matsumura et al., 2005
hepatocyte growth factor activator Q04756 373-655 peptide-Arg407+Ile-peptide P Leu Arg Pro Arg Ile Ile Gly Gly Mukai et al., 2008 20892
Inhibin alpha chain precursor P05111 1-366 peptide-Arg232+Ser-peptide P CS Arg Ala Arg Arg Ser Thr Pro Leu Matsumura et al., 2005
Inhibin beta A chain precursor P08476 1-426 peptide-Arg310+Gly-peptide P CS Arg Arg Arg Arg Gly Leu Glu Cys Matsumura et al., 2005
Inhibin beta C chain precursor P55103 1-352 peptide-Arg236+Gly-peptide P CS Ile His Arg Arg Gly Ile Asp Cys Matsumura et al., 2005
Inter-alpha-trypsin inhibitor heavy chain H1 precursor P19827 1-672 peptide-Arg34+Ser-peptide P CS Ala Thr Gly Arg Ser Lys Ser Ser Matsumura et al., 2005
Inter-alpha-trypsin inhibitor heavy chain H2 precursor P19823 1-702 peptide-Arg54+Ser-peptide P CS Arg Tyr Gln Arg Ser Leu Pro Gly Matsumura et al., 2005
Inter-alpha-trypsin inhibitor heavy chain H3 precursor Q06033 1-890 peptide-Arg33+Ser-peptide P CS Leu Gly Lys Arg Ser Leu Pro Glu Matsumura et al., 2005
Interleukin-1 alpha precursor P01583 1-271 peptide-Arg112+Ser-peptide P CS Ile Lys Pro Arg Ser Ala Pro Phe Matsumura et al., 2005
Kallikrein-1 precursor P06870 19-262 peptide-Arg24+Ile-peptide P NT Ile Gln Ser Arg Ile Val Gly Gly Yoon et al., 2007
Kallikrein-11 precursor Q9UBX7 51-282 Glu-Thr-Arg53+Ile-peptide P NT - Glu Thr Arg Ile Ile Lys Gly Yoon et al., 2007
Kallikrein-12 precursor Q9UKR0 18-248 Ala-Thr-Pro-Lys21+Ile-peptide P NT Ala Thr Pro Lys Ile Phe Asn Gly Yoon et al., 2007
Kallikrein-13 precursor Q9UKR3 17-277 peptide-Lys25+Val-peptide P NT Glu Ser Ser Lys Val Leu Asn Thr Yoon et al., 2007
Kallikrein-14 precursor Q9P0G3 35-267 peptide-Lys40+Ile-peptide P NT Asp Glu Asn Lys Ile Ile Gly Gly Yoon et al., 2007
Kallikrein-15 precursor Q9H2R5 17-256 peptide-Lys21+Leu-peptide P NT Asp Gly Asp Lys Leu Leu Glu Gly Yoon et al., 2007
Kallikrein-2 precursor P20151 19-261 peptide-Arg24+Ile-peptide P NT Ile Gln Ser Arg Ile Val Gly Gly Yoon et al., 2007
Kallikrein-5 precursor Q9Y337 23-293 peptide-Arg66+Ile-peptide P NT Ser Ser Ser Arg Ile Ile Asn Gly Yoon et al., 2007
Kallikrein-6 precursor Q92876 17-244 peptide-Lys21+Leu-peptide P NT Glu Gln Asn Lys Leu Val His Gly Yoon et al., 2007
Kallikrein-9 precursor Q9UKQ9 16-250 peptide-Arg22+Ala-peptide P NT Ala Asp Thr Arg Ala Ile Gly Ala Yoon et al., 2007
kallikrein-related peptidase 3 P07288 17-261 peptide-Arg24+Ile-peptide P Ile Leu Ser Arg Ile Val Gly Gly Debela et al., 2006 21054
memapsin-2 precursor P56817 1-501 peptide-Arg45+Glu-peptide P CS Arg Leu Pro Arg Glu Thr Asp Glu Matsumura et al., 2005
Meprin A subunit alpha precursor Q16819 1-746 peptide-Arg65+Asn-peptide P CS Gln Lys Ser Arg Asn Gly Leu Arg Matsumura et al., 2005
Meprin A subunit beta Q16820 1-701 Peptide-Arg61+Asn-Peptide P - Ala Gln Ile Arg Asn Ser Ile Ile Debela et al., 2008
Neuroendocrine convertase 1 precursor P29120 1-753 peptide-Arg110+Ser-peptide P CS Arg Ser Lys Arg Ser Ala Leu Arg Matsumura et al., 2005
Neuroendocrine convertase 2 precursor P16519 1-638 peptide-Arg109+Gly-peptide P CS Arg Lys Lys Arg Gly Tyr Arg Asp Matsumura et al., 2005
Neurotrophin-3 precursor P20783 1-257 peptide-Arg138+Tyr-peptide P CS Arg Arg Lys Arg Tyr Ala Glu His Matsumura et al., 2005
Neurotrophin-4 precursor P34130 1-210 peptide-Arg80+Gly-peptide P CS Arg Ser Arg Arg Gly Val Ser Glu Matsumura et al., 2005
Pappalysin-1 precursor Q13219 1-1627 peptide-Arg81+Glu-peptide P CS Arg Gln Gln Arg Glu Ala Arg Gly Matsumura et al., 2005
Parathyroid hormone precursor P01270 1-115 peptide-Arg31+Ser-peptide P CS Val Lys Lys Arg Ser Val Ser Glu Matsumura et al., 2005
Parathyroid hormone-related protein precursor P12272 1-177 peptide-Arg36+Ala-peptide P CS Arg Lys Lys Arg Ala Val Ser Glu Matsumura et al., 2005
Phospholipase A2 precursor P04054 1-148 peptide-Arg22+Ala-peptide P CS Ile Ser Pro Arg Ala Val Trp Gln Matsumura et al., 2005
Platelet basic protein precursor P02775 1-128 peptide-Arg43+Asn-peptide P CS Gln Thr Lys Arg Asn Leu Ala Lys Matsumura et al., 2005
Platelet-derived growth factor subunit A precursor P04085 1-211 peptide-Arg86+Ser-peptide P CS Arg Arg Lys Arg Ser Ile Glu Glu Matsumura et al., 2005
prostatic acid phosphatase P15309 33-386 peptide-Arg105+Ser-peptide P Val Tyr Ile Arg Ser Thr Asp Val Takayama et al., 2001 20246
Protachykinin-1 precursor P20366 1-126 peptide-Arg57+Arg-peptide P CS Arg Ile Ala Arg Arg Pro Lys Pro Matsumura et al., 2005
Proteinase-activated receptor 2 precursor P55085 1-397 peptide-Arg36+Ser-peptide P CS Ser Lys Gly Arg Ser Leu Ile Gly Matsumura et al., 2005
Prothrombin precursor P00734 1-622 peptide-Arg327+Thr-peptide P CS Phe Asn Pro Arg Thr Phe Gly Ser Matsumura et al., 2005
Renin precursor P00797 1-406 peptide-Arg66+Leu-peptide P CS Pro Met Lys Arg Leu Thr Leu Gly Matsumura et al., 2005
S-2222 Bz-Ile-Glu-Gly-Arg+NHPhNO2 S Ile Glu Gly Arg NAN - - - Takayama et al., 2001
S-2251 Val-Leu-Lys+NHPhNO2 S - Val Leu Lys NAN - - - Takayama et al., 2001
S-2266 Val-Leu-Arg+NHPhNO2 S - Val Leu Arg NAN - - - Takayama et al., 2001
S-2302 D-Pro-Phe-Arg+NHPhNO2 S - DPr Phe Arg NAN - - - Takayama et al., 2001
Serum albumin precursor P02768 1-609 peptide-Arg24+Asp-peptide P CS Val Phe Arg Arg Asp Ala His Lys Matsumura et al., 2005
Somatostatin precursor P61278 1-116 peptide-Arg88+Ser-peptide P CS Glu Leu Gln Arg Ser Ala Asn Ser Matsumura et al., 2005
Somatotropin P01241 27-217 Peptide-Arg160+Thr-Peptide P NT Gly Ser Pro Arg Thr Gly Gln Ile Komatsu et al., 2007
Transforming growth factor beta-1 precursor P01137 1-390 peptide-Arg278+Ala-peptide P CS Arg His Arg Arg Ala Leu Asp Thr Matsumura et al., 2005
Transforming growth factor beta-2 precursor P61812-1 1-414 peptide-Arg302+Ala-peptide P CS Arg Lys Lys Arg Ala Leu Asp Ala Matsumura et al., 2005
Transforming growth factor beta-2 precursor P61812-2 1-442 peptide-Arg330+Ala-peptide P CS Arg Lys Lys Arg Ala Leu Asp Ala Matsumura et al., 2005
urokinase plasminogen activator, urokinase receptor isoform 1 precursor Q03405 102-117 peptide-Tyr109+Ser-peptide P Ala Val Thr Tyr Ser Arg Ser Arg Beaufort et al., 2006 19800
urokinase plasminogen activator, urokinase receptor isoform 1 precursor Q03405 102-117 peptide-Arg111+Ser-peptide P Thr Tyr Ser Arg Ser Arg Tyr Leu Beaufort et al., 2006 19801
urokinase plasminogen activator, urokinase receptor isoform 1 precursor Q03405 294-305 peptide-Arg303+Ser-peptide P Val Gln Tyr Arg Ser Gly Ala Ala Beaufort et al., 2006 19802
Val-Leu-Lys-NHMec Val-Leu-Lys-NHMec S - Val Leu Lys AMC - - - Obiezu et al., 2006
Val-Pro-Arg-NHMec Val-Pro-Arg+NHMec S - Val Pro Arg AMC - - - Obiezu et al., 2006
Vascular endothelial growth factor C precursor P49767 1-419 peptide-Arg102+Thr-peptide P CS Leu Asn Ser Arg Thr Glu Glu Thr Matsumura et al., 2005
Vitamin K-dependent protein C precursor P04070 1-461 peptide-Arg211+Leu-peptide P CS Val Asp Pro Arg Leu Ile Asp Gly Matsumura et al., 2005
Vitamin K-dependent protein S precursor P07225 1-676 peptide-Arg41+Ala-peptide P CS Arg Lys Arg Arg Ala Asn Ser Leu Matsumura et al., 2005
Vitamin K-dependent protein Z precursor P22891 1-400 peptide-Arg40+Ala-peptide P CS Arg Trp Lys Arg Ala Gly Ser Tyr Matsumura et al., 2005
Z-Leu-Arg-NHMec Z-Leu-Arg+NHMec S - Z Leu Arg AMC - - - Debela et al., 2006
Z-Phe-Arg-NHMec Z-Phe-Arg+NHMec S - Z Phe Arg AMC - - - Debela et al., 2006

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