$VAR1 = undef;

Summary for peptidase S01.251: kallikrein-related peptidase 4

Names
MEROPS Name	kallikrein-related peptidase 4
Other names	enamel matrix serine proteinase 1, EMSP1, kallikrein hK4, kallikrein 4 (Homo sapiens), kallikrein-like 1 protein (Homo sapiens), KLK4 g.p. (Homo sapiens), Klk4 g.p. (Mus musculus), prostase, PRSS17 [obs.] g.p. (Homo sapiens), serine protease 17 [obs.]

Domain architecture

MEROPS Classification
Classification	Clan PA >> Subclan PA(S) >> Family S1 >> Subfamily A >> S01.251
Holotype	kallikrein-related peptidase 4 (Homo sapiens), Uniprot accession Q9Y5K2 (peptidase unit: 31-254), MERNUM MER0005266
History	Identifier created: MEROPS 3.02 (25 June 1998)

Activity
Catalytic type	Serine
Peplist	Included in the Peplist with identifier PL00326
NC-IUBMB	Not yet included in IUBMB recommendations.
Proteolytic events	CutDB database (10 cleavages)
Activity status	human: active (Simmer, 2004) mouse: active (by similarity) (Hu et al., 2000)
Physiology	Mutation in prostase causes autosomal recessive hypomaturation amelogenesis imperfecta (Hart et al., 2004).
Pharmaceutical relevance	Proposed for use in vaccine strategies against prostate cancer (Hural et al., 2002).
Other databases	WIKIPEDIA	http://en.wikipedia.org/wiki/KLK4
Cleavage site specificity	Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern	r/-/-/R-/-/-/- (based on 123 cleavages)

Specificity matrix

Amino acid	P4	P3	P2	P1	P1'	P2'	P3'	P4'
Gly	6	4	8	1	12	8	14	27
Pro	9	2	14	0	1	6	10	12
Ala	5	4	6	1	18	13	3	4
Val	7	10	2	0	6	16	5	5
Leu	9	8	6	3	6	17	6	7
Ile	7	4	5	0	10	12	4	5
Met	0	5	2	2	3	1	2	1
Phe	5	4	3	1	3	4	2	3
Tyr	4	5	3	7	1	4	6	2
Trp	2	1	2	0	1	9	1	1
Ser	7	14	16	0	23	4	13	6
Thr	2	7	8	0	3	6	1	7
Cys	0	0	0	0	1	0	1	2
Asn	4	3	3	2	3	3	11	2
Gln	2	17	5	2	4	3	6	6
Asp	5	2	1	0	5	0	12	3
Glu	3	6	2	0	6	2	8	8
Lys	1	11	23	9	2	3	2	6
Arg	34	7	12	92	3	4	4	4
His	0	3	1	3	4	0	3	3

Specificity from combinatorial peptides

Organism	comment	P4	P3	P2	P1	P1'	P2'	P3'	P4'	optimal substrate	fluorophore or acceptor-donor pair	Reference
Homo sapiens	recombinant	I/V	S/broad	Q/L/V	R/F	-	-	-	-	ISQR	ACC	Borgo�o et al., 2007
Homo sapiens	recombinant	I/broad	broad	Q/V/L	R	-	-	-	-	IbQR	ACC	Debela et al., 2006