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Substrates for peptidase M12.032: LAST peptidase (Limulus-type)

Summary Alignment Tree Sequences Sequence features Distribution Literature Substrates

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate Uniprot Residue range Cleavage Site Cleavage type Evidence P4 P3 P2 P1 P1' P2' P3' P4' Reference CutDB MERNUM
26S proteasome non-ATPase regulatory subunit 2 Q13200 16-31 Ser-Pro-Ala-Ala19+Ala-Peptide N MS Ser Pro Ala Ala Ala Pro Gly Gly Becker-Pauly et al., 2011
40S ribosomal protein S26 P62854 102-115 Arg-Pro-Ala-Gly105+Ala-Peptide N MS Arg Pro Ala Gly Ala Ala Pro Arg Becker-Pauly et al., 2011
60 kDa heat shock protein, mitochondrial P10809 322-344 Gly-Ala-Val-Phe325+Gly-Peptide N MS Gly Ala Val Phe Gly Glu Glu Gly Becker-Pauly et al., 2011
60 kDa heat shock protein, mitochondrial P10809 144-157 Val-Met-Leu-Ala147+Val-Peptide N MS Val Met Leu Ala Val Asp Ala Val Becker-Pauly et al., 2011
60S acidic ribosomal protein P0 P05388 289-301 Pro-Ala-Ala-Ala292+Ala-Peptide N MS Pro Ala Ala Ala Ala Ala Pro Ala Becker-Pauly et al., 2011
60S acidic ribosomal protein P0 P05388 275-301 Phe-Val-Ala-Ala278+Ala-Peptide N MS Phe Val Ala Ala Ala Pro Val Ala Becker-Pauly et al., 2011
60S acidic ribosomal protein P2 P05387 77-94 Pro-Gly-Ser-Ala80+Ala-Peptide N MS Pro Gly Ser Ala Ala Pro Ala Ala Becker-Pauly et al., 2011
60S ribosomal protein L29 P47914 135-149 Ala-Gln-Ala-Ala138+Ala-Peptide N MS Ala Gln Ala Ala Ala Pro Ala Ser Becker-Pauly et al., 2011
Aspartate aminotransferase, mitochondrial P00505 35-53 His-Val-Glu-Met38+Gly-Peptide N MS His Val Glu Met Gly Pro Pro Asp Becker-Pauly et al., 2011
Bifunctional coenzyme A synthase Q13057 393-417 His-Arg-Ala-Tyr396+Ala-Peptide N MS His Arg Ala Tyr Ala Pro Gly Gly Becker-Pauly et al., 2011
Calpain small subunit 1 P04632 86-99 His-Tyr-Ser-Asn89+IIe-Peptide N MS His Tyr Ser Asn Ile Glu Ala Asn Becker-Pauly et al., 2011
Coiled-coil-helix-coiled-coil-helix domain-containing protein 2, mitochondrial Q9Y6H1 34-51 Pro-Pro-Ala-Ala37+Ala-Peptide N MS Pro Pro Ala Ala Ala Pro Pro Ser Becker-Pauly et al., 2011
Core histone macro-H2A.1 O75367 274-292 Ile-His-Cys-Asn277+Ser-Peptide N MS Ile His Cys Asn Ser Pro Val Trp Becker-Pauly et al., 2011
Creatine kinase M-type P06732 186-209 Gln-Leu-Ile-Asp189+Asp-Peptide N MS Gln Leu Ile Asp Asp His Phe Leu Becker-Pauly et al., 2011
Cystatin-B P04080 1-24 Met-Met-Cys-Gly4+Ala-Peptide N MS Met Met Cys Gly Ala Pro Ser Ala Becker-Pauly et al., 2011
DJ-1 putative peptidase Q99497 104-122 Ala-Ile-Cys-Ala107+Gly-Peptide N MS Ala Ile Cys Ala Gly Pro Thr Ala Becker-Pauly et al., 2011
Elongation factor 1-alpha 1 P68104 277-290 Val-Val-Thr-Phe280+Ala-Peptide N MS Val Val Thr Phe Ala Pro Val Asn Becker-Pauly et al., 2011
Elongation factor 1-alpha 1 P68104 263-290 Pro-Val-Gly-Arg266+Val-Peptide N MS Pro Val Gly Arg Val Glu Thr Gly Becker-Pauly et al., 2011
Elongation factor 1-alpha 2 Q05639 277-290 Val-Val-Thr-Phe280+Ala-Peptide N MS Val Val Thr Phe Ala Pro Val Asn Becker-Pauly et al., 2011
Elongation factor 1-gamma P26641 30-45 Arg-Val-Leu-Ser33+Ala-Peptide N MS Arg Val Leu Ser Ala Pro Pro His Becker-Pauly et al., 2011
Elongation factor 2 P13639 612-625 Gly-Leu-Ala-Glu615+Asp-Peptide N MS Gly Leu Ala Glu Asp Ile Asp Lys Becker-Pauly et al., 2011
Eukaryotic translation initiation factor 5A-1-like Q6IS14 70-86 Glu-Asp-Ile-Cys73+Pro-Peptide N MS Glu Asp Ile Cys Pro Ser Thr His Becker-Pauly et al., 2011
F-actin-capping protein subunit alpha-2 P47755 20-37 Phe-Ile-Ile-His23+Ala-Peptide N MS Phe Ile Ile His Ala Pro Pro Gly Becker-Pauly et al., 2011
Filamin-A P21333 2544-2559 Ala-Pro-Gln-His2547+Gly-Peptide N MS Ala Pro Gln His Gly Ala Pro Gly Becker-Pauly et al., 2011
Glutathione S-transferase omega-1 P78417 10-25 Gly-Lys-Gly-Ser13+Ala-Peptide N MS Gly Lys Gly Ser Ala Pro Pro Gly Becker-Pauly et al., 2011
Glyceraldehyde-3-phosphate dehydrogenase P04406 119-139 Val-Ile-Ile-Ser122+Ala-Peptide N MS Val Ile Ile Ser Ala Pro Ser Ala Becker-Pauly et al., 2011
Glyceraldehyde-3-phosphate dehydrogenase P04406 124-139 Pro-Ser-Ala-Asp127+Ala-Peptide N MS Pro Ser Ala Asp Ala Pro Met Phe Becker-Pauly et al., 2011
Heat shock 70 kDa protein 1A/1B P08107 518-533 Met-Val-Gln-Glu521+Ala-Peptide N MS Met Val Gln Glu Ala Glu Lys Tyr Becker-Pauly et al., 2011
Heat shock 70 kDa protein 1A/1B P08107 521-533 Glu-Ala-Glu-Lys524+Tyr-Peptide N MS Glu Ala Glu Lys Tyr Lys Ala Glu Becker-Pauly et al., 2011
Heat shock 70 kDa protein 1A/1B P08107 578-595 Ile-Ser-Trp-Leu581+Asp-Peptide N MS Ile Ser Trp Leu Asp Ala Asn Thr Becker-Pauly et al., 2011
Heat shock 70 kDa protein 1A/1B P08107 609-628 Gly-Leu-Tyr-Gln612+Gly-Peptide N MS Gly Leu Tyr Gln Gly Ala Gly Gly Becker-Pauly et al., 2011
Heat shock 70 kDa protein 1A/1B P08107 612-628 Gln-Gly-Ala-Gly615+Gly-Peptide N MS Gln Gly Ala Gly Gly Pro Gly Pro Becker-Pauly et al., 2011
Heat shock 70 kDa protein 1A/1B P08107 77-88 Lys-Phe-Gly-Asp80+Pro-Peptide N MS Lys Phe Gly Asp Pro Val Val Gln Becker-Pauly et al., 2011
Heat shock 70 kDa protein 1A/1B P08107 73-88 Leu-Ile-Gly-Arg76+Lys-Peptide N MS Leu Ile Gly Arg Lys Phe Gly Asp Becker-Pauly et al., 2011
Heat shock 70 kDa protein 1A/1B P08107 53-71 Asp-Ala-Ala-Lys56+Asn-Peptide N MS Asp Ala Ala Lys Asn Gln Val Ala Becker-Pauly et al., 2011
Heat shock 70 kDa protein 1A/1B P08107 85-102 Ser-Asp-Met-Lys88+His-Peptide N MS Ser Asp Met Lys His Trp Pro Phe Becker-Pauly et al., 2011
Heat shock 70 kDa protein 1A/1B P08107 325-342 Lys-Leu-Asp-Lys328+Ala-Peptide N MS Lys Leu Asp Lys Ala Gln Ile His Becker-Pauly et al., 2011
Heat shock 70 kDa protein 1A/1B P08107 322-342 Arg-Asp-Ala-Lys325+Leu-Peptide N MS Arg Asp Ala Lys Leu Asp Lys Ala Becker-Pauly et al., 2011
Heat shock 70 kDa protein 1A/1B P08107 327-342 Asp-Lys-Ala-Gln330+IIe-Peptide N MS Asp Lys Ala Gln Ile His Asp Leu Becker-Pauly et al., 2011
Heat shock 70 kDa protein 1A/1B P08107 233-247 Phe-Asp-Asn-Arg236+Leu-Peptide N MS Phe Asp Asn Arg Leu Val Asn His Becker-Pauly et al., 2011
Heat shock 70 kDa protein 1A/1B P08107 536-550 Val-Ser-Ala-Lys539+Asn-Peptide N MS Val Ser Ala Lys Asn Ala Leu Glu Becker-Pauly et al., 2011
Heat shock cognate 71 kDa protein P11142 221-236 Ser-Thr-Ala-Gly224+Asp-Peptide N MS Ser Thr Ala Gly Asp Thr His Leu Becker-Pauly et al., 2011
Heat shock protein HSP 90-beta P08238 621-639 Met-Ala-Lys-Lys624+His-Peptide N MS Met Ala Lys Lys His Leu Glu Ile Becker-Pauly et al., 2011
Heat shock protein HSP 90-beta P08238 79-95 Pro-Gln-Glu-Arg82+Thr-Peptide N MS Pro Gln Glu Arg Thr Leu Thr Leu Becker-Pauly et al., 2011
High mobility group nucleosome-binding domain-containing protein 3 Q15651 31-44 Ser-Ala-Lys-Pro34+Ala-Peptide N MS Ser Ala Lys Pro Ala Pro Pro Lys Becker-Pauly et al., 2011
Histone H1.2 P16403 8-21 Ala-Pro-Ala-Ala11+Ala-Peptide N MS Ala Pro Ala Ala Ala Pro Pro Ala Becker-Pauly et al., 2011
Histone H1.4 P10412 7-21 Ala-Ala-Pro-Ala10+Ala-Peptide N MS Ala Ala Pro Ala Ala Pro Ala Pro Becker-Pauly et al., 2011
Histone H1.4 P10412 4-21 Thr-Ala-Pro-Ala7+Ala-Peptide N MS Thr Ala Pro Ala Ala Pro Ala Ala Becker-Pauly et al., 2011
Histone H1.5 P16401 36-49 Arg-Lys-Ala-Thr39+Gly-Peptide N MS Arg Lys Ala Thr Gly Pro Pro Val Becker-Pauly et al., 2011
Lupus La protein P05455 367-384 Asp-Asp-Glu-His370+Asp-Peptide N MS Asp Asp Glu His Asp Glu His Asp Becker-Pauly et al., 2011
Malate dehydrogenase, mitochondrial P40926 57-74 Asp-Ile-Ala-His60+Thr-Peptide N MS Asp Ile Ala His Thr Pro Gly Val Becker-Pauly et al., 2011
Methyltransferase-like protein 13 Q8N6R0-2 192-205 Gly-Glu-Pro-Arg195+Tyr-Peptide N MS Gly Glu Pro Arg Tyr Thr Leu His Becker-Pauly et al., 2011
Mitochondrial-processing peptidase subunit beta O75439 239-257 Ile-Val-Leu-Ala242+Ala-Peptide N MS Ile Val Leu Ala Ala Ala Gly Gly Becker-Pauly et al., 2011
Monocarboxylate transporter 3 O95907 213-230 Ala-Gly-Asp-Ala216+Pro-Peptide N MS Ala Gly Asp Ala Pro Gly Glu Ala Becker-Pauly et al., 2011
Nucleoside diphosphate kinase B P22392 3-18 Asn-Leu-Glu-Arg6+Thr-Peptide N MS Asn Leu Glu Arg Thr Phe Ile Ala Becker-Pauly et al., 2011
Peptidyl-prolyl cis-trans isomerase A P62937 76-91 Lys-Ser-Ile-Tyr79+Gly-Peptide N MS Lys Ser Ile Tyr Gly Glu Lys Phe Becker-Pauly et al., 2011
Peptidyl-prolyl cis-trans isomerase FKBP4 Q02790 12-28 Gly-Ala-Gln-Ser15+Ala-Peptide N MS Gly Ala Gln Ser Ala Pro Leu Pro Becker-Pauly et al., 2011
Peroxiredoxin-5, mitochondrial P30044 87-102 Gly-Val-Leu-Phe90+Gly-Peptide N MS Gly Val Leu Phe Gly Val Pro Gly Becker-Pauly et al., 2011
Poly [ADP-ribose] polymerase 1 P09874 372-394 Ser-Thr-Ala-Ser375+Ala-Peptide N MS Ser Thr Ala Ser Ala Pro Ala Ala Becker-Pauly et al., 2011
Protein-L-isoaspartate(D-aspartate) O-methyltransferase P22061 158-178 Val-Gly-Ala-Ala161+Ala-Peptide N MS Val Gly Ala Ala Ala Pro Val Val Becker-Pauly et al., 2011
Pyruvate kinase isozymes M1/M2 P14618 19-32 His-Ala-Ala-Met22+Ala-Peptide N MS His Ala Ala Met Ala Asp Thr Phe Becker-Pauly et al., 2011
Pyruvate kinase isozymes M1/M2 P14618 16-32 Gln-Gln-Leu-His19+Ala-Peptide N MS Gln Gln Leu His Ala Ala Met Ala Becker-Pauly et al., 2011
Reticulon-4 Q9NQC3 164-177 Pro-Ala-Pro-Ala167+Ala-Peptide N MS Pro Ala Pro Ala Ala Pro Pro Ser Becker-Pauly et al., 2011
RNA-binding protein EWS Q01844 616-632 Gly-Gly-Pro-Gly619+Gly-Peptide N MS Gly Gly Pro Gly Gly Pro Pro Gly Becker-Pauly et al., 2011
Splicing factor 3B subunit 2 Q13435 119-134 Phe-Pro-Met-Ala122+His-Peptide N MS Phe Pro Met Ala His Pro Pro Asn Becker-Pauly et al., 2011
Splicing factor, proline- and glutamine-rich P23246 186-199 Pro-Pro-Pro-Ala189+Ala-Peptide N MS Pro Pro Pro Ala Ala Val Pro Gly Becker-Pauly et al., 2011
Superoxide dismutase [Cu-Zn] P00441 58-70 Cys-Thr-Ser-Ala61+Gly-Peptide N MS Cys Thr Ser Ala Gly Pro His Phe Becker-Pauly et al., 2011
Superoxide dismutase [Cu-Zn] P00441 9-24 Leu-Lys-Gly-Asp12+Gly-Peptide N MS Leu Lys Gly Asp Gly Pro Val Gln Becker-Pauly et al., 2011
Transcription intermediary factor 1-beta Q13263 14-32 Ser-Ala-Ala-Ser17+Gly-Peptide N MS Ser Ala Ala Ser Gly Ser Pro Gly Becker-Pauly et al., 2011
Trifunctional purine biosynthetic protein adenosine-3 P22102 91-108 Val-Gln-Cys-Phe94+Gly-Peptide N MS Val Gln Cys Phe Gly Pro Thr Ala Becker-Pauly et al., 2011
Tubulin alpha-4A chain P68366 269-280 Leu-Ala-Thr-Tyr272+Ala-Peptide N MS Leu Ala Thr Tyr Ala Pro Val Ile Becker-Pauly et al., 2011
Tubulin alpha-4A chain P68366 267-280 Phe-Pro-Leu-Ala270+Thr-Peptide N MS Phe Pro Leu Ala Thr Tyr Ala Pro Becker-Pauly et al., 2011
Tubulin alpha-4A chain P68366 290-304 Glu-Ile-Thr-Asn293+Ala-Peptide N MS Glu Ile Thr Asn Ala Cys Phe Glu Becker-Pauly et al., 2011
Tubulin alpha-8 chain Q9NY65 84-105 Arg-Gln-Leu-Phe87+His-Peptide N MS Arg Gln Leu Phe His Pro Glu Gln Becker-Pauly et al., 2011
Tubulin beta chain P07437 22-46 Glu-Val-Ile-Ser25+Asp-Peptide N MS Glu Val Ile Ser Asp Glu His Gly Becker-Pauly et al., 2011
Tubulin beta chain P07437 100-122 Asn-Trp-Ala-Lys103+Gly-Peptide N MS Asn Trp Ala Lys Gly His Tyr Thr Becker-Pauly et al., 2011
Ubiquitin-like modifier-activating enzyme 1 P22314 350-368 Arg-Asn-Glu-Glu353+Asp-Peptide N MS Arg Asn Glu Glu Asp Ala Ala Glu Becker-Pauly et al., 2011
WASH complex subunit 7 Q2M389 741-755 Thr-Thr-Val-Ala744+Leu-Peptide N MS Thr Thr Val Ala Leu His Asp Trp Becker-Pauly et al., 2011

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