Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 1 |
unknown peptidase |
1-724 |
P |
|
NT |
<%Agarwal et al., 2012[]%> |
| 27 |
matrix metallopeptidase-2 |
24-37 |
N |
|
MS |
Schilling & Overall, 2008 |
| 36 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 37 |
glutamyl endopeptidase I |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 41 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 42 |
matrix metallopeptidase-2 |
41-56 |
N |
|
MS |
Schilling & Overall, 2008 |
| 44 |
elastase-2 |
41-55 |
N |
|
MS |
Schilling & Overall, 2008 |
| 45 |
cathepsin L |
42-57 |
N |
|
MS |
Biniossek et al., 2011 |
| 45 |
cathepsin S |
42-55 |
N |
|
MS |
Biniossek et al., 2011 |
| 46 |
matrix metallopeptidase-2 |
42-57 |
N |
|
MS |
Schilling & Overall, 2008 |
| 52 |
peptidyl-Lys metallopeptidase |
1-724 |
N |
|
MS |
|
| 53 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 55 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 56 |
chymotrypsin A (cattle-type) |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 57 |
meprin beta subunit |
54-69 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 57 |
glutamyl endopeptidase I |
56-69 |
N |
|
MS |
Schilling & Overall, 2008 |
| 59 |
cathepsin G |
56-69 |
N |
|
MS |
Schilling & Overall, 2008 |
| 63 |
peptidyl-Lys metallopeptidase |
1-724 |
N |
|
MS |
|
| 64 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 68 |
peptidyl-Lys metallopeptidase |
1-724 |
N |
|
MS |
|
| 69 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 72 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 82 |
LAST peptidase (Limulus-type) |
79-95 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 82 |
LAST_MAM peptidase (Limulus-type) |
79-95 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 82 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 94 |
peptidyl-Lys metallopeptidase |
1-724 |
N |
|
MS |
|
| 95 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 97 |
matrix metallopeptidase-2 |
95-107 |
N |
|
MS |
Schilling & Overall, 2008 |
| 101 |
matrix metallopeptidase-2 |
97-115 |
N |
|
MS |
Schilling & Overall, 2008 |
| 104 |
matrix metallopeptidase-2 |
97-115 |
N |
|
MS |
Schilling & Overall, 2008 |
| 106 |
peptidyl-Lys metallopeptidase |
1-724 |
N |
|
MS |
|
| 107 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 113 |
chymotrypsin A (cattle-type) |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 115 |
glutamyl endopeptidase I |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 117 |
chymotrypsin A (cattle-type) |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 177 |
thrombin |
175-187 |
N |
|
MS |
Schilling & Overall, 2008 |
| 180 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 184 |
cathepsin L |
181-196 |
N |
|
MS |
Biniossek et al., 2011 |
| 184 |
matrix metallopeptidase-2 |
180-196 |
N |
|
MS |
Schilling & Overall, 2008 |
| 185 |
elastase-2 |
180-196 |
N |
|
MS |
Schilling & Overall, 2008 |
| 186 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 187 |
glutamyl endopeptidase I |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 196 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 198 |
peptidyl-Lys metallopeptidase |
1-724 |
N |
|
MS |
|
| 199 |
lysyl endopeptidase (bacteria) |
1-724 |
N |
|
MS |
|
| 204 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 207 |
matrix metallopeptidase-2 |
204-219 |
N |
|
MS |
Schilling & Overall, 2008 |
| 219 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 249 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 259 |
caspase-3 |
2-724 |
P |
|
CS |
Ju et al., 2007 |
| 263 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 266 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 272 |
peptidyl-Lys metallopeptidase |
1-724 |
N |
|
MS |
|
| 273 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 274 |
peptidyl-Lys metallopeptidase |
1-724 |
N |
|
MS |
|
| 275 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 283 |
peptidyl-Lys metallopeptidase |
1-724 |
N |
|
MS |
|
| 284 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 291 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 294 |
meprin alpha subunit |
291-306 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 305 |
peptidyl-Lys metallopeptidase |
1-724 |
N |
|
MS |
|
| 306 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 310 |
cathepsin L |
307-319 |
N |
|
MS |
Biniossek et al., 2011 |
| 310 |
meprin alpha subunit |
307-319 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 310 |
LAST_MAM peptidase (Limulus-type) |
307-319 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 318 |
peptidyl-Lys metallopeptidase |
1-724 |
N |
|
MS |
|
| 319 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 322 |
cathepsin L |
320-330 |
N |
|
MS |
Biniossek et al., 2011 |
| 322 |
cathepsin S |
320-330 |
N |
|
MS |
Biniossek et al., 2011 |
| 330 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 337 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 359 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 378 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 392 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 399 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 410 |
peptidyl-Lys metallopeptidase |
1-724 |
N |
|
MS |
|
| 411 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 414 |
matrix metallopeptidase-2 |
411-427 |
N |
|
MS |
Schilling & Overall, 2008 |
| 415 |
HIV-1 retropepsin |
411-427 |
N |
|
MS |
Schilling & Overall, 2008 |
| 416 |
astacin |
413-428 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 416 |
elastase-2 |
411-428 |
N |
|
MS |
Schilling & Overall, 2008 |
| 416 |
cathepsin G |
411-428 |
N |
|
MS |
Schilling & Overall, 2008 |
| 417 |
cathepsin L |
412-426 |
N |
|
MS |
Biniossek et al., 2011 |
| 417 |
cathepsin S |
412-426 |
N |
|
MS |
Biniossek et al., 2011 |
| 422 |
peptidyl-Lys metallopeptidase |
1-724 |
N |
|
MS |
|
| 427 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 428 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 435 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 466 |
HIV-1 retropepsin |
456-475 |
N |
|
MS |
Schilling & Overall, 2008 |
| 475 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 480 |
peptidyl-Lys metallopeptidase |
1-724 |
N |
|
MS |
|
| 490 |
peptidyl-Lys metallopeptidase |
1-724 |
N |
|
MS |
|
| 491 |
meprin beta subunit |
488-503 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 512 |
HIV-1 retropepsin |
506-526 |
N |
|
MS |
Schilling & Overall, 2008 |
| 526 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 531 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 537 |
peptidyl-Lys metallopeptidase |
1-724 |
N |
|
MS |
|
| 538 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 549 |
peptidyl-Lys metallopeptidase |
1-724 |
N |
|
MS |
|
| 550 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 551 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 552 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 606 |
peptidyl-Lys metallopeptidase |
1-724 |
N |
|
MS |
|
| 607 |
lysyl endopeptidase (bacteria) |
1-724 |
N |
|
MS |
|
| 612 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 622 |
peptidyl-Lys metallopeptidase |
1-724 |
N |
|
MS |
|
| 623 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 624 |
LAST peptidase (Limulus-type) |
621-639 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 625 |
meprin alpha subunit |
2-724 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 627 |
cathepsin L |
625-639 |
N |
|
MS |
Biniossek et al., 2011 |
| 627 |
cathepsin S |
625-636 |
N |
|
MS |
Biniossek et al., 2011 |
| 627 |
meprin alpha subunit |
2-724 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 627 |
meprin beta subunit |
624-639 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 627 |
LAST_MAM peptidase (Limulus-type) |
624-639 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 630 |
meprin alpha subunit |
627-639 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 630 |
meprin beta subunit |
627-639 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 631 |
meprin alpha subunit |
628-639 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 631 |
granzyme B (Homo sapiens-type) |
2-724 |
P |
|
MS |
Hostetter et al., 2007 |
| 640 |
peptidyl-Lys metallopeptidase |
1-724 |
N |
|
MS |
|
| 662 |
matrix metallopeptidase-11 |
2-724 |
N |
|
|
Kleifeld et al., 2010 |
| 663 |
cathepsin B |
653-676 |
N |
|
MS |
Biniossek et al., 2011 |
| 685 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 693 |
granzyme B (Homo sapiens-type) |
2-724 |
P |
|
MS |
Hostetter et al., 2007 |
| 719 |
trypsin 1 |
2-724 |
N |
|
MS |
Schilling & Overall, 2008 |
