Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 1 |
methionyl aminopeptidase 2 |
1-858 |
P |
|
MS |
Warder et al., 2008 |
| 14 |
peptidyl-Lys metallopeptidase |
1-858 |
N |
|
MS |
|
| 31 |
peptidyl-Lys metallopeptidase |
1-858 |
N |
|
MS |
|
| 41 |
peptidyl-Lys metallopeptidase |
1-858 |
N |
|
MS |
|
| 42 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 50 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 60 |
peptidyl-Lys metallopeptidase |
1-858 |
N |
|
MS |
|
| 71 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 76 |
matrix metallopeptidase-2 |
71-90 |
N |
|
MS |
Schilling & Overall, 2008 |
| 79 |
cathepsin L |
72-90 |
N |
|
MS |
Biniossek et al., 2011 |
| 80 |
cathepsin L |
72-90 |
N |
|
MS |
Biniossek et al., 2011 |
| 81 |
HIV-1 retropepsin |
71-90 |
N |
|
MS |
Schilling & Overall, 2008 |
| 81 |
cathepsin G |
71-90 |
N |
|
MS |
Schilling & Overall, 2008 |
| 90 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 93 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 136 |
cathepsin L |
121-144 |
N |
|
MS |
Biniossek et al., 2011 |
| 155 |
matrix metallopeptidase-2 |
149-166 |
N |
|
MS |
Schilling & Overall, 2008 |
| 166 |
cathepsin L |
163-175 |
N |
|
MS |
Biniossek et al., 2011 |
| 166 |
glutamyl endopeptidase I |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 217 |
matrix metallopeptidase-2 |
206-230 |
N |
|
MS |
Schilling & Overall, 2008 |
| 230 |
glutamyl endopeptidase I |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 271 |
peptidyl-Lys metallopeptidase |
1-858 |
N |
|
MS |
|
| 287 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 299 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 308 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 317 |
peptidyl-Lys metallopeptidase |
1-858 |
N |
|
MS |
|
| 327 |
peptidyl-Lys metallopeptidase |
1-858 |
N |
|
MS |
|
| 353 |
HIV-1 retropepsin |
342-366 |
N |
|
MS |
Schilling & Overall, 2008 |
| 365 |
peptidyl-Lys metallopeptidase |
1-858 |
N |
|
MS |
|
| 366 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 374 |
cathepsin L |
369-386 |
N |
|
MS |
Biniossek et al., 2011 |
| 385 |
peptidyl-Lys metallopeptidase |
1-858 |
N |
|
MS |
|
| 391 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 400 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 428 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 430 |
cathepsin L |
429-439 |
N |
|
MS |
Biniossek et al., 2011 |
| 430 |
cathepsin S |
429-439 |
N |
|
MS |
Biniossek et al., 2011 |
| 438 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 449 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 456 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 468 |
matrix metallopeptidase-2 |
456-481 |
N |
|
MS |
Schilling & Overall, 2008 |
| 470 |
elastase-2 |
456-481 |
N |
|
MS |
Schilling & Overall, 2008 |
| 471 |
HIV-1 retropepsin |
456-481 |
N |
|
MS |
Schilling & Overall, 2008 |
| 471 |
matrix metallopeptidase-2 |
456-481 |
N |
|
MS |
Schilling & Overall, 2008 |
| 480 |
peptidyl-Lys metallopeptidase |
1-858 |
N |
|
MS |
|
| 481 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 489 |
glutamyl endopeptidase I |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 495 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 497 |
peptidyl-Lys metallopeptidase |
1-858 |
N |
|
MS |
|
| 498 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 506 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 540 |
granzyme B (Homo sapiens-type) |
2-858 |
N |
|
MS |
Van Damme et al., 2010 |
| 558 |
peptidyl-Lys metallopeptidase |
1-858 |
N |
|
MS |
|
| 562 |
cathepsin S |
560-571 |
N |
|
MS |
Biniossek et al., 2011 |
| 562 |
cathepsin K |
559-571 |
N |
|
MS |
Schilling & Overall, 2008 |
| 563 |
meprin beta subunit |
560-572 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 571 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 580 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 594 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 613 |
cathepsin G |
605-625 |
N |
|
MS |
Schilling & Overall, 2008 |
| 614 |
cathepsin S |
608-625 |
N |
|
MS |
Biniossek et al., 2011 |
| 614 |
cathepsin K |
605-625 |
N |
|
MS |
Schilling & Overall, 2008 |
| 615 |
meprin alpha subunit |
612-625 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 615 |
meprin beta subunit |
612-625 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 615 |
LAST peptidase (Limulus-type) |
612-625 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 615 |
LAST_MAM peptidase (Limulus-type) |
612-625 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 616 |
meprin alpha subunit |
613-625 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 625 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 636 |
cathepsin L |
634-647 |
N |
|
MS |
Biniossek et al., 2011 |
| 636 |
cathepsin S |
634-647 |
N |
|
MS |
Biniossek et al., 2011 |
| 636 |
cathepsin K |
633-647 |
N |
|
MS |
Schilling & Overall, 2008 |
| 637 |
glutamyl endopeptidase I |
633-647 |
N |
|
MS |
Schilling & Overall, 2008 |
| 638 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 647 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 648 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 651 |
cathepsin S |
649-663 |
N |
|
MS |
Biniossek et al., 2011 |
| 667 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 674 |
glutamyl endopeptidase I |
667-688 |
N |
|
MS |
Schilling & Overall, 2008 |
| 676 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 688 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 771 |
HIV-1 retropepsin |
767-785 |
N |
|
MS |
Schilling & Overall, 2008 |
| 771 |
meprin beta subunit |
768-785 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 771 |
LAST_MAM peptidase (Limulus-type) |
768-785 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 772 |
meprin beta subunit |
769-785 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 772 |
LAST_MAM peptidase (Limulus-type) |
769-785 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 773 |
glutamyl endopeptidase I |
767-785 |
N |
|
MS |
Schilling & Overall, 2008 |
| 774 |
meprin beta subunit |
771-785 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 780 |
matrix metallopeptidase-2 |
773-792 |
N |
|
MS |
Schilling & Overall, 2008 |
| 782 |
matrix metallopeptidase-2 |
773-792 |
N |
|
MS |
Schilling & Overall, 2008 |
| 785 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 792 |
glutamyl endopeptidase I |
785-801 |
N |
|
MS |
Schilling & Overall, 2008 |
| 797 |
cathepsin L |
786-813 |
N |
|
MS |
Biniossek et al., 2011 |
| 801 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 818 |
cathepsin L |
802-835 |
N |
|
MS |
Biniossek et al., 2011 |
| 825 |
meprin beta subunit |
822-839 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 825 |
cathepsin G |
801-839 |
N |
|
MS |
Schilling & Overall, 2008 |
| 827 |
LAST_MAM peptidase (Limulus-type) |
824-839 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 839 |
trypsin 1 |
2-858 |
N |
|
MS |
Schilling & Overall, 2008 |
| 844 |
peptidyl-Lys metallopeptidase |
1-858 |
N |
|
MS |
|
| 857 |
carboxypeptidase A4 |
2-858 |
N |
|
MS |
Van Damme et al., 2010 |
