Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 1 |
unknown peptidase |
1-641 |
P |
|
NT |
<%Agarwal et al., 2012[]%> |
| 13 |
matrix metallopeptidase-2 |
10-27 |
N |
|
MS |
Schilling & Overall, 2008 |
| 16 |
matrix metallopeptidase-2 |
7-25 |
N |
|
MS |
Schilling & Overall, 2008 |
| 21 |
cathepsin G |
3-36 |
N |
|
MS |
Schilling & Overall, 2008 |
| 25 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 27 |
glutamyl endopeptidase I |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 36 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 49 |
matrix metallopeptidase-2 |
48-69 |
N |
|
MS |
Schilling & Overall, 2008 |
| 49 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 52 |
cathepsin L |
37-69 |
N |
|
MS |
Biniossek et al., 2011 |
| 52 |
meprin beta subunit |
2-641 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 53 |
matrix metallopeptidase-2 |
50-68 |
N |
|
MS |
Schilling & Overall, 2008 |
| 55 |
peptidyl-Lys metallopeptidase |
1-641 |
N |
|
MS |
|
| 56 |
LAST peptidase (Limulus-type) |
53-71 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 56 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 60 |
cathepsin L |
57-71 |
N |
|
MS |
Biniossek et al., 2011 |
| 60 |
cathepsin S |
57-71 |
N |
|
MS |
Biniossek et al., 2011 |
| 60 |
cathepsin B |
57-71 |
N |
|
MS |
Biniossek et al., 2011 |
| 61 |
meprin beta subunit |
2-641 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 61 |
LAST_MAM peptidase (Limulus-type) |
58-71 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 68 |
chymotrypsin A (cattle-type) |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 69 |
glutamyl endopeptidase I |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 70 |
peptidyl-Lys metallopeptidase |
1-641 |
N |
|
MS |
|
| 71 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 72 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 76 |
LAST peptidase (Limulus-type) |
73-88 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 76 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 77 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 79 |
cathepsin K |
77-88 |
N |
|
MS |
Schilling & Overall, 2008 |
| 79 |
LAST_MAM peptidase (Limulus-type) |
76-88 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 80 |
HIV-1 retropepsin |
77-88 |
N |
|
MS |
Schilling & Overall, 2008 |
| 80 |
LAST peptidase (Limulus-type) |
77-88 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 87 |
peptidyl-Lys metallopeptidase |
1-641 |
N |
|
MS |
|
| 88 |
LAST peptidase (Limulus-type) |
85-102 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 88 |
LAST_MAM peptidase (Limulus-type) |
85-102 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 88 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 91 |
LAST_MAM peptidase (Limulus-type) |
88-102 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 92 |
meprin alpha subunit |
89-102 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 92 |
LAST_MAM peptidase (Limulus-type) |
89-102 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 92 |
elastase-2 |
88-102 |
N |
|
MS |
Schilling & Overall, 2008 |
| 92 |
cathepsin G |
88-102 |
N |
|
MS |
Schilling & Overall, 2008 |
| 93 |
cathepsin L |
89-102 |
N |
|
MS |
Biniossek et al., 2011 |
| 93 |
cathepsin S |
89-102 |
N |
|
MS |
Biniossek et al., 2011 |
| 93 |
cathepsin K |
88-102 |
N |
|
MS |
Schilling & Overall, 2008 |
| 93 |
cathepsin B |
89-102 |
N |
|
MS |
Biniossek et al., 2011 |
| 93 |
matrix metallopeptidase-2 |
88-102 |
N |
|
MS |
Schilling & Overall, 2008 |
| 93 |
meprin alpha subunit |
90-102 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 101 |
peptidyl-Lys metallopeptidase |
1-641 |
N |
|
MS |
|
| 102 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 111 |
peptidyl-Lys metallopeptidase |
1-641 |
N |
|
MS |
|
| 114 |
meprin alpha subunit |
111-126 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 114 |
meprin beta subunit |
111-126 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 118 |
meprin alpha subunit |
115-126 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 125 |
peptidyl-Lys metallopeptidase |
1-641 |
N |
|
MS |
|
| 128 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 132 |
glutamyl endopeptidase I |
128-155 |
N |
|
MS |
Schilling & Overall, 2008 |
| 133 |
matrix metallopeptidase-2 |
128-155 |
N |
|
MS |
Schilling & Overall, 2008 |
| 134 |
HIV-1 retropepsin |
128-155 |
N |
|
MS |
Schilling & Overall, 2008 |
| 142 |
matrix metallopeptidase-2 |
141-155 |
N |
|
MS |
Schilling & Overall, 2008 |
| 155 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 159 |
meprin beta subunit |
156-171 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 159 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 160 |
HIV-1 retropepsin |
159-171 |
N |
|
MS |
Schilling & Overall, 2008 |
| 160 |
caspase-3 |
159-171 |
N |
|
MS |
Schilling & Overall, 2008 |
| 160 |
caspase-7 |
159-171 |
N |
|
MS |
Schilling & Overall, 2008 |
| 160 |
LAST_MAM peptidase (Limulus-type) |
157-171 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 160 |
glutamyl endopeptidase I |
159-171 |
N |
|
MS |
Schilling & Overall, 2008 |
| 166 |
matrix metallopeptidase-2 |
160-192 |
N |
|
MS |
Schilling & Overall, 2008 |
| 171 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 180 |
matrix metallopeptidase-2 |
160-192 |
N |
|
MS |
Schilling & Overall, 2008 |
| 192 |
glutamyl endopeptidase I |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 220 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 227 |
cathepsin G |
221-236 |
N |
|
MS |
Schilling & Overall, 2008 |
| 236 |
LAST peptidase (Limulus-type) |
233-247 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 236 |
LAST_MAM peptidase (Limulus-type) |
233-247 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 236 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 238 |
elastase-2 |
236-247 |
N |
|
MS |
Schilling & Overall, 2008 |
| 239 |
cathepsin L |
237-246 |
N |
|
MS |
Biniossek et al., 2011 |
| 239 |
cathepsin S |
237-246 |
N |
|
MS |
Biniossek et al., 2011 |
| 246 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 247 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 299 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 301 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 304 |
matrix metallopeptidase-2 |
301-311 |
N |
|
MS |
Schilling & Overall, 2008 |
| 311 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 325 |
LAST peptidase (Limulus-type) |
322-342 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 328 |
astacin |
325-342 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 328 |
LAST peptidase (Limulus-type) |
325-342 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 328 |
LAST_MAM peptidase (Limulus-type) |
325-342 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 328 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 330 |
LAST peptidase (Limulus-type) |
327-342 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 333 |
cathepsin B |
329-342 |
N |
|
MS |
Biniossek et al., 2011 |
| 342 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 347 |
peptidyl-Lys metallopeptidase |
1-641 |
N |
|
MS |
|
| 351 |
meprin beta subunit |
348-361 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 360 |
peptidyl-Lys metallopeptidase |
1-641 |
N |
|
MS |
|
| 361 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 366 |
granzyme B (Homo sapiens-type) |
2-641 |
P |
|
CS |
Loeb et al., 2006 |
| 370 |
matrix metallopeptidase-2 |
361-384 |
N |
|
MS |
Schilling & Overall, 2008 |
| 371 |
LAST_MAM peptidase (Limulus-type) |
368-384 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 372 |
glutamyl endopeptidase I |
361-384 |
N |
|
MS |
Schilling & Overall, 2008 |
| 374 |
matrix metallopeptidase-2 |
361-384 |
N |
|
MS |
Schilling & Overall, 2008 |
| 384 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 386 |
glutamyl endopeptidase I |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 392 |
chymotrypsin A (cattle-type) |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 398 |
matrix metallopeptidase-2 |
384-415 |
N |
|
MS |
Schilling & Overall, 2008 |
| 400 |
matrix metallopeptidase-2 |
384-415 |
N |
|
MS |
Schilling & Overall, 2008 |
| 402 |
HIV-1 retropepsin |
384-415 |
N |
|
MS |
Schilling & Overall, 2008 |
| 402 |
matrix metallopeptidase-2 |
384-415 |
N |
|
MS |
Schilling & Overall, 2008 |
| 403 |
matrix metallopeptidase-2 |
384-415 |
N |
|
MS |
Schilling & Overall, 2008 |
| 403 |
meprin beta subunit |
400-415 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 404 |
cathepsin L |
385-415 |
N |
|
MS |
Biniossek et al., 2011 |
| 404 |
meprin beta subunit |
401-415 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 404 |
glutamyl endopeptidase I |
384-415 |
N |
|
MS |
Schilling & Overall, 2008 |
| 415 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 416 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 438 |
matrix metallopeptidase-2 |
423-447 |
N |
|
MS |
Schilling & Overall, 2008 |
| 447 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 484 |
matrix metallopeptidase-2 |
469-497 |
N |
|
MS |
Schilling & Overall, 2008 |
| 497 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 506 |
glutamyl endopeptidase I |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 511 |
peptidyl-Lys metallopeptidase |
1-641 |
N |
|
MS |
|
| 520 |
cathepsin K |
517-533 |
N |
|
MS |
Schilling & Overall, 2008 |
| 521 |
meprin alpha subunit |
2-641 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 521 |
meprin beta subunit |
2-641 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 521 |
LAST peptidase (Limulus-type) |
518-533 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 521 |
LAST_MAM peptidase (Limulus-type) |
518-533 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 523 |
peptidyl-Lys metallopeptidase |
1-641 |
N |
|
MS |
|
| 524 |
LAST peptidase (Limulus-type) |
521-533 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 524 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 525 |
peptidyl-Lys metallopeptidase |
1-641 |
N |
|
MS |
|
| 533 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 535 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 538 |
peptidyl-Lys metallopeptidase |
1-641 |
N |
|
MS |
|
| 539 |
meprin beta subunit |
536-550 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 539 |
LAST peptidase (Limulus-type) |
536-550 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 539 |
LAST_MAM peptidase (Limulus-type) |
536-550 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 539 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 540 |
matrix metallopeptidase-2 |
539-550 |
N |
|
MS |
Schilling & Overall, 2008 |
| 548 |
matrix metallopeptidase-2 |
543-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 549 |
peptidyl-Lys metallopeptidase |
1-641 |
N |
|
MS |
|
| 550 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 554 |
meprin alpha subunit |
551-568 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 556 |
glutamyl endopeptidase I |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 558 |
peptidyl-Lys metallopeptidase |
1-641 |
N |
|
MS |
|
| 559 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 571 |
meprin alpha subunit |
2-641 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 571 |
meprin beta subunit |
2-641 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 572 |
peptidyl-Lys metallopeptidase |
1-641 |
N |
|
MS |
|
| 575 |
LAST_MAM peptidase (Limulus-type) |
572-589 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 576 |
glutamyl endopeptidase I |
573-589 |
N |
|
MS |
Schilling & Overall, 2008 |
| 577 |
elastase-2 |
573-595 |
N |
|
MS |
Schilling & Overall, 2008 |
| 578 |
elastase-2 |
573-589 |
N |
|
MS |
Schilling & Overall, 2008 |
| 579 |
HIV-1 retropepsin |
573-595 |
N |
|
MS |
Schilling & Overall, 2008 |
| 580 |
matrix metallopeptidase-2 |
573-595 |
N |
|
MS |
Schilling & Overall, 2008 |
| 580 |
astacin |
577-595 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 580 |
LAST_MAM peptidase (Limulus-type) |
577-595 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 581 |
cathepsin L |
574-595 |
N |
|
MS |
Biniossek et al., 2011 |
| 581 |
LAST peptidase (Limulus-type) |
578-595 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 581 |
LAST_MAM peptidase (Limulus-type) |
578-595 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 582 |
astacin |
579-595 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 582 |
LAST_MAM peptidase (Limulus-type) |
579-595 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 584 |
meprin alpha subunit |
2-641 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 587 |
cathepsin S |
574-595 |
N |
|
MS |
Biniossek et al., 2011 |
| 588 |
peptidyl-Lys metallopeptidase |
1-641 |
N |
|
MS |
|
| 589 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 591 |
glutamyl endopeptidase I |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 595 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 597 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 600 |
glutamyl endopeptidase I |
597-628 |
N |
|
MS |
Schilling & Overall, 2008 |
| 602 |
cathepsin G |
597-628 |
N |
|
MS |
Schilling & Overall, 2008 |
| 603 |
meprin beta subunit |
600-628 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 606 |
elastase-2 |
597-628 |
N |
|
MS |
Schilling & Overall, 2008 |
| 606 |
cathepsin G |
597-628 |
N |
|
MS |
Schilling & Overall, 2008 |
| 607 |
elastase-2 |
597-628 |
N |
|
MS |
Schilling & Overall, 2008 |
| 608 |
elastase-2 |
597-628 |
N |
|
MS |
Schilling & Overall, 2008 |
| 609 |
matrix metallopeptidase-2 |
597-628 |
N |
|
MS |
Schilling & Overall, 2008 |
| 610 |
HIV-1 retropepsin |
597-628 |
N |
|
MS |
Schilling & Overall, 2008 |
| 610 |
cathepsin G |
597-628 |
N |
|
MS |
Schilling & Overall, 2008 |
| 611 |
meprin beta subunit |
608-628 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 611 |
LAST_MAM peptidase (Limulus-type) |
608-628 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 611 |
cathepsin G |
597-628 |
N |
|
MS |
Schilling & Overall, 2008 |
| 612 |
cathepsin B |
598-628 |
N |
|
MS |
Biniossek et al., 2011 |
| 612 |
LAST peptidase (Limulus-type) |
609-628 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 614 |
elastase-2 |
597-628 |
N |
|
MS |
Schilling & Overall, 2008 |
| 615 |
cathepsin B |
598-628 |
N |
|
MS |
Biniossek et al., 2011 |
| 615 |
meprin alpha subunit |
612-628 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 615 |
LAST peptidase (Limulus-type) |
612-628 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 615 |
LAST_MAM peptidase (Limulus-type) |
612-628 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 617 |
LAST_MAM peptidase (Limulus-type) |
614-628 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 620 |
cathepsin B |
598-628 |
N |
|
MS |
Biniossek et al., 2011 |
| 620 |
meprin alpha subunit |
617-628 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 621 |
matrix metallopeptidase-2 |
611-641 |
N |
|
MS |
Schilling & Overall, 2008 |
| 628 |
trypsin 1 |
1-641 |
N |
|
MS |
Schilling & Overall, 2008 |
