Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 2 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 3 |
glutamyl endopeptidase I |
2-19 |
N |
|
MS |
Schilling & Overall, 2008 |
| 5 |
elastase-2 |
2-19 |
N |
|
MS |
Schilling & Overall, 2008 |
| 5 |
cathepsin G |
2-19 |
N |
|
MS |
Schilling & Overall, 2008 |
| 8 |
cathepsin L |
3-22 |
N |
|
MS |
Biniossek et al., 2011 |
| 8 |
cathepsin S |
3-19 |
N |
|
MS |
Biniossek et al., 2011 |
| 19 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 24 |
HtrA2 peptidase |
1-444 |
P |
|
|
Vande Walle et al., 2007 |
| 25 |
LAST peptidase (Limulus-type) |
22-46 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 26 |
matrix metallopeptidase-2 |
19-46 |
N |
|
MS |
Schilling & Overall, 2008 |
| 39 |
granzyme B (Homo sapiens-type) |
1-444 |
N |
|
MS |
Van Damme et al., 2010 |
| 46 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 47 |
HtrA2 peptidase |
1-444 |
P |
|
|
Vande Walle et al., 2007 |
| 58 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 62 |
granzyme A |
1-444 |
N |
|
|
Van Damme et al., 2010 |
| 62 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 63 |
matrix metallopeptidase-2 |
59-81 |
N |
|
MS |
Schilling & Overall, 2008 |
| 66 |
HtrA2 peptidase |
1-444 |
P |
|
|
Vande Walle et al., 2007 |
| 77 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 81 |
chymotrypsin A (cattle-type) |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 82 |
matrix metallopeptidase-2 |
77-103 |
N |
|
MS |
Schilling & Overall, 2008 |
| 83 |
matrix metallopeptidase-2 |
77-103 |
N |
|
MS |
Schilling & Overall, 2008 |
| 84 |
elastase-2 |
77-103 |
N |
|
MS |
Schilling & Overall, 2008 |
| 89 |
matrix metallopeptidase-2 |
77-103 |
N |
|
MS |
Schilling & Overall, 2008 |
| 89 |
cathepsin G |
77-103 |
N |
|
MS |
Schilling & Overall, 2008 |
| 90 |
cathepsin G |
77-103 |
N |
|
MS |
Schilling & Overall, 2008 |
| 101 |
HtrA2 peptidase |
1-444 |
P |
|
|
Vande Walle et al., 2007 |
| 103 |
LAST peptidase (Limulus-type) |
100-122 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 103 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 106 |
LAST_MAM peptidase (Limulus-type) |
103-122 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 107 |
LAST_MAM peptidase (Limulus-type) |
104-122 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 107 |
HtrA2 peptidase |
1-444 |
P |
|
|
Vande Walle et al., 2007 |
| 108 |
cathepsin K |
103-122 |
N |
|
MS |
Schilling & Overall, 2008 |
| 108 |
LAST_MAM peptidase (Limulus-type) |
105-122 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 108 |
glutamyl endopeptidase I |
103-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 109 |
HIV-1 retropepsin |
103-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 111 |
HIV-1 retropepsin |
103-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 111 |
matrix metallopeptidase-2 |
103-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 111 |
glutamyl endopeptidase I |
103-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 112 |
HIV-1 retropepsin |
103-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 112 |
cathepsin G |
103-122 |
N |
|
MS |
Schilling & Overall, 2008 |
| 113 |
elastase-2 |
103-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 114 |
HIV-1 retropepsin |
103-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 114 |
cathepsin L |
104-122 |
N |
|
MS |
Biniossek et al., 2011 |
| 114 |
caspase-1 |
1-444 |
N |
|
MS |
Agard et al., 2010 |
| 114 |
caspase-3 |
103-122 |
N |
|
MS |
Schilling & Overall, 2008 |
| 114 |
granzyme B (Homo sapiens-type) |
1-444 |
N |
|
MS |
Van Damme et al., 2010 |
| 121 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 122 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 135 |
HtrA2 peptidase |
1-444 |
P |
|
|
Vande Walle et al., 2007 |
| 137 |
cathepsin G |
122-154 |
N |
|
MS |
Schilling & Overall, 2008 |
| 138 |
matrix metallopeptidase-2 |
122-154 |
N |
|
MS |
Schilling & Overall, 2008 |
| 139 |
HtrA2 peptidase |
1-444 |
P |
|
|
Vande Walle et al., 2007 |
| 144 |
glutamyl endopeptidase I |
122-154 |
N |
|
MS |
Schilling & Overall, 2008 |
| 154 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 162 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 173 |
matrix metallopeptidase-2 |
172-183 |
N |
|
MS |
Schilling & Overall, 2008 |
| 174 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 183 |
chymotrypsin A (cattle-type) |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 186 |
matrix metallopeptidase-2 |
177-198 |
N |
|
MS |
Schilling & Overall, 2008 |
| 198 |
glutamyl endopeptidase I |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 205 |
granzyme B (Homo sapiens-type) |
1-444 |
N |
|
MS |
Van Damme et al., 2010 |
| 217 |
HtrA2 peptidase |
1-444 |
P |
|
|
Vande Walle et al., 2007 |
| 221 |
HtrA2 peptidase |
1-444 |
P |
|
|
Vande Walle et al., 2007 |
| 224 |
glutamyl endopeptidase I |
223-241 |
N |
|
MS |
Schilling & Overall, 2008 |
| 227 |
matrix metallopeptidase-2 |
223-241 |
N |
|
MS |
Schilling & Overall, 2008 |
| 229 |
HtrA2 peptidase |
1-444 |
P |
|
|
Vande Walle et al., 2007 |
| 231 |
matrix metallopeptidase-2 |
223-241 |
N |
|
MS |
Schilling & Overall, 2008 |
| 240 |
HtrA2 peptidase |
1-444 |
P |
|
|
Vande Walle et al., 2007 |
| 241 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 251 |
granzyme A |
1-444 |
N |
|
|
Van Damme et al., 2010 |
| 251 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 252 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 253 |
HtrA2 peptidase |
1-444 |
P |
|
|
Vande Walle et al., 2007 |
| 262 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 263 |
HtrA2 peptidase |
1-444 |
P |
|
|
Vande Walle et al., 2007 |
| 282 |
granzyme A |
1-444 |
N |
|
|
Van Damme et al., 2010 |
| 282 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 288 |
glutamyl endopeptidase I |
282-297 |
N |
|
MS |
Schilling & Overall, 2008 |
| 292 |
HtrA2 peptidase |
1-444 |
P |
|
|
Vande Walle et al., 2007 |
| 297 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 304 |
granzyme B (Homo sapiens-type) |
1-444 |
N |
|
MS |
Van Damme et al., 2010 |
| 309 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 318 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 323 |
peptidyl-Lys metallopeptidase |
1-444 |
N |
|
MS |
|
| 324 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 335 |
peptidyl-Lys metallopeptidase |
1-444 |
N |
|
MS |
|
| 336 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 339 |
matrix metallopeptidase-2 |
336-350 |
N |
|
MS |
Schilling & Overall, 2008 |
| 349 |
peptidyl-Lys metallopeptidase |
1-444 |
N |
|
MS |
|
| 350 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 359 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 361 |
peptidyl-Lys metallopeptidase |
1-444 |
N |
|
MS |
|
| 362 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 378 |
peptidyl-Lys metallopeptidase |
1-444 |
N |
|
MS |
|
| 379 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 380 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 390 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 392 |
lysyl endopeptidase (bacteria) |
1-444 |
N |
|
MS |
|
