Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 23 |
matrix metallopeptidase-2 |
16-32 |
N |
|
MS |
Schilling & Overall, 2008 |
| 32 |
trypsin 1 |
1-408 |
N |
|
MS |
Schilling & Overall, 2008 |
| 41 |
trypsin 1 |
1-408 |
N |
|
MS |
Schilling & Overall, 2008 |
| 54 |
trypsin 1 |
1-408 |
N |
|
MS |
Schilling & Overall, 2008 |
| 60 |
trypsin 1 |
1-408 |
N |
|
MS |
Schilling & Overall, 2008 |
| 74 |
trypsin 1 |
1-408 |
N |
|
MS |
Schilling & Overall, 2008 |
| 76 |
trypsin 1 |
1-408 |
N |
|
MS |
Schilling & Overall, 2008 |
| 78 |
meprin alpha subunit |
74-88 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 79 |
HIV-1 retropepsin |
76-88 |
N |
|
MS |
Schilling & Overall, 2008 |
| 86 |
trypsin 1 |
1-408 |
N |
|
MS |
Schilling & Overall, 2008 |
| 88 |
trypsin 1 |
1-408 |
N |
|
MS |
Schilling & Overall, 2008 |
| 98 |
matrix metallopeptidase-2 |
91-109 |
N |
|
MS |
Schilling & Overall, 2008 |
| 99 |
cathepsin K |
91-109 |
N |
|
MS |
Schilling & Overall, 2008 |
| 99 |
glutamyl endopeptidase I |
91-109 |
N |
|
MS |
Schilling & Overall, 2008 |
| 109 |
trypsin 1 |
1-408 |
N |
|
MS |
Schilling & Overall, 2008 |
| 111 |
thrombin |
107-129 |
N |
|
MS |
Schilling & Overall, 2008 |
| 129 |
glutamyl endopeptidase I |
1-408 |
N |
|
MS |
Schilling & Overall, 2008 |
| 151 |
trypsin 1 |
1-408 |
N |
|
MS |
Schilling & Overall, 2008 |
| 165 |
trypsin 1 |
1-408 |
N |
|
MS |
Schilling & Overall, 2008 |
| 176 |
trypsin 1 |
1-408 |
N |
|
MS |
Schilling & Overall, 2008 |
| 180 |
HIV-1 retropepsin |
176-191 |
N |
|
MS |
Schilling & Overall, 2008 |
| 185 |
trypsin 1 |
1-408 |
N |
|
MS |
Schilling & Overall, 2008 |
| 191 |
trypsin 1 |
1-408 |
N |
|
MS |
Schilling & Overall, 2008 |
| 192 |
trypsin 1 |
1-408 |
N |
|
MS |
Schilling & Overall, 2008 |
| 220 |
granzyme B (Homo sapiens-type) |
1-408 |
P |
|
|
Casciola-Rosen et al., 1999 |
| 232 |
matrix metallopeptidase-2 |
228-247 |
N |
|
MS |
Schilling & Overall, 2008 |
| 247 |
glutamyl endopeptidase I |
1-408 |
N |
|
MS |
Schilling & Overall, 2008 |
| 253 |
meprin beta subunit |
1-408 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 269 |
trypsin 1 |
1-408 |
N |
|
MS |
Schilling & Overall, 2008 |
| 276 |
trypsin 1 |
1-408 |
N |
|
MS |
Schilling & Overall, 2008 |
| 299 |
trypsin 1 |
1-408 |
N |
|
MS |
Schilling & Overall, 2008 |
| 307 |
LAST_MAM peptidase (Limulus-type) |
304-317 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 312 |
trypsin 1 |
1-408 |
N |
|
MS |
Schilling & Overall, 2008 |
| 316 |
peptidyl-Lys metallopeptidase |
1-408 |
N |
|
MS |
|
| 317 |
trypsin 1 |
1-408 |
N |
|
MS |
Schilling & Overall, 2008 |
| 327 |
peptidyl-Lys metallopeptidase |
1-408 |
N |
|
MS |
|
| 328 |
trypsin 1 |
1-408 |
N |
|
MS |
Schilling & Overall, 2008 |
| 364 |
granzyme H |
1-408 |
P |
|
|
Romero et al., 2008 |
| 370 |
LAST peptidase (Limulus-type) |
367-384 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 371 |
caspase-3 |
1-408 |
P |
|
CS |
Ju et al., 2007 |
| 373 |
LAST_MAM peptidase (Limulus-type) |
370-384 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 374 |
caspase-3 |
1-408 |
P |
|
CS |
Ju et al., 2007 |
