Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 9 |
HIV-1 retropepsin |
5-20 |
N |
|
MS |
Schilling & Overall, 2008 |
| 11 |
elastase-2 |
5-20 |
N |
|
MS |
Schilling & Overall, 2008 |
| 19 |
peptidyl-Lys metallopeptidase |
1-462 |
N |
|
MS |
|
| 20 |
trypsin 1 |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 23 |
cathepsin K |
20-30 |
N |
|
MS |
Schilling & Overall, 2008 |
| 29 |
peptidyl-Lys metallopeptidase |
1-462 |
N |
|
MS |
|
| 30 |
trypsin 1 |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 84 |
trypsin 1 |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 96 |
trypsin 1 |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 110 |
matrix metallopeptidase-2 |
108-129 |
N |
|
MS |
Schilling & Overall, 2008 |
| 113 |
matrix metallopeptidase-2 |
108-129 |
N |
|
MS |
Schilling & Overall, 2008 |
| 129 |
trypsin 1 |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 134 |
trypsin 1 |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 137 |
matrix metallopeptidase-2 |
134-146 |
N |
|
MS |
Schilling & Overall, 2008 |
| 145 |
peptidyl-Lys metallopeptidase |
1-462 |
N |
|
MS |
|
| 146 |
trypsin 1 |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 147 |
matrix metallopeptidase-2 |
135-168 |
N |
|
MS |
Schilling & Overall, 2008 |
| 153 |
peptidyl-Lys metallopeptidase |
1-462 |
N |
|
MS |
|
| 162 |
chymotrypsin A (cattle-type) |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 163 |
matrix metallopeptidase-2 |
154-172 |
N |
|
MS |
Schilling & Overall, 2008 |
| 164 |
peptidyl-Lys metallopeptidase |
1-462 |
N |
|
MS |
|
| 168 |
glutamyl endopeptidase I |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 169 |
HIV-1 retropepsin |
166-179 |
N |
|
MS |
Schilling & Overall, 2008 |
| 169 |
glutamyl endopeptidase I |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 172 |
trypsin 1 |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 179 |
trypsin 1 |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 194 |
matrix metallopeptidase-2 |
180-212 |
N |
|
MS |
Schilling & Overall, 2008 |
| 195 |
matrix metallopeptidase-2 |
180-212 |
N |
|
MS |
Schilling & Overall, 2008 |
| 201 |
cathepsin G |
200-212 |
N |
|
MS |
Schilling & Overall, 2008 |
| 212 |
trypsin 1 |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 225 |
meprin alpha subunit |
1-462 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 229 |
meprin alpha subunit |
1-462 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 229 |
LAST_MAM peptidase (Limulus-type) |
226-247 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 243 |
peptidyl-Lys metallopeptidase |
1-462 |
N |
|
MS |
|
| 246 |
elastase-2 |
219-255 |
N |
|
MS |
Schilling & Overall, 2008 |
| 247 |
trypsin 1 |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 253 |
HtrA2 peptidase |
1-462 |
P |
|
|
Vande Walle et al., 2007 |
| 254 |
matrix metallopeptidase-2 |
252-268 |
N |
|
MS |
Schilling & Overall, 2008 |
| 255 |
matrix metallopeptidase-2 |
252-268 |
N |
|
MS |
Schilling & Overall, 2008 |
| 255 |
trypsin 1 |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 266 |
matrix metallopeptidase-2 |
254-280 |
N |
|
MS |
Schilling & Overall, 2008 |
| 266 |
LAST peptidase (Limulus-type) |
263-290 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 266 |
trypsin 1 |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 268 |
glutamyl endopeptidase I |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 272 |
peptidyl-Lys metallopeptidase |
1-462 |
N |
|
MS |
|
| 280 |
LAST peptidase (Limulus-type) |
277-290 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 280 |
LAST_MAM peptidase (Limulus-type) |
277-290 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 280 |
chymotrypsin A (cattle-type) |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 280 |
cathepsin G |
271-290 |
N |
|
MS |
Schilling & Overall, 2008 |
| 289 |
peptidyl-Lys metallopeptidase |
1-462 |
N |
|
MS |
|
| 290 |
trypsin 1 |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 354 |
cathepsin L |
331-367 |
N |
|
MS |
Biniossek et al., 2011 |
| 362 |
caspase-1 |
1-462 |
N |
|
MS |
Agard et al., 2010 |
| 362 |
matrix metallopeptidase-2 |
361-372 |
N |
|
MS |
Schilling & Overall, 2008 |
| 372 |
chymotrypsin A (cattle-type) |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 384 |
peptidyl-Lys metallopeptidase |
1-462 |
N |
|
MS |
|
| 385 |
trypsin 1 |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 392 |
trypsin 1 |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 393 |
matrix metallopeptidase-2 |
392-403 |
N |
|
MS |
Schilling & Overall, 2008 |
| 394 |
peptidyl-Lys metallopeptidase |
1-462 |
N |
|
MS |
|
| 395 |
trypsin 1 |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 398 |
granzyme B (Homo sapiens-type) |
1-462 |
P |
|
MS |
Van Damme et al., 2009 |
| 403 |
caspase-1 |
1-462 |
N |
|
MS |
Agard et al., 2010 |
| 403 |
glutamyl endopeptidase I |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 413 |
granzyme B (Homo sapiens-type) |
1-462 |
N |
|
MS |
Van Damme et al., 2010 |
| 413 |
glutamyl endopeptidase I |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
| 423 |
trypsin 1 |
1-462 |
N |
|
MS |
Schilling & Overall, 2008 |
