Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 23 |
HIV-1 retropepsin |
16-40 |
N |
|
MS |
Schilling & Overall, 2008 |
| 25 |
HIV-1 retropepsin |
16-40 |
N |
|
MS |
Schilling & Overall, 2008 |
| 27 |
cathepsin L |
3-40 |
N |
|
MS |
Biniossek et al., 2011 |
| 27 |
cathepsin S |
3-40 |
N |
|
MS |
Biniossek et al., 2011 |
| 27 |
cathepsin K |
2-40 |
N |
|
MS |
Schilling & Overall, 2008 |
| 27 |
glutamyl endopeptidase I |
2-40 |
N |
|
MS |
Schilling & Overall, 2008 |
| 28 |
astacin |
25-40 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 40 |
trypsin 1 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 87 |
cathepsin L |
85-96 |
N |
|
MS |
Biniossek et al., 2011 |
| 87 |
cathepsin S |
85-96 |
N |
|
MS |
Biniossek et al., 2011 |
| 90 |
glutamyl endopeptidase I |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 91 |
matrix metallopeptidase-2 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 92 |
cathepsin G |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 93 |
elastase-2 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 93 |
cathepsin G |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 94 |
cathepsin L |
85-105 |
N |
|
MS |
Biniossek et al., 2011 |
| 95 |
peptidyl-Lys metallopeptidase |
1-448 |
N |
|
MS |
|
| 96 |
trypsin 1 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 103 |
chymotrypsin A (cattle-type) |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 105 |
trypsin 1 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 111 |
peptidyl-Lys metallopeptidase |
1-448 |
N |
|
MS |
|
| 156 |
trypsin 1 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 163 |
trypsin 1 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 190 |
cathepsin L |
168-201 |
N |
|
MS |
Biniossek et al., 2011 |
| 199 |
matrix metallopeptidase-2 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 202 |
HIV-1 retropepsin |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 214 |
trypsin 1 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 215 |
trypsin 1 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 229 |
trypsin 1 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 243 |
trypsin 1 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 252 |
HIV-1 retropepsin |
243-264 |
N |
|
MS |
Schilling & Overall, 2008 |
| 264 |
trypsin 1 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 269 |
cathepsin G |
264-280 |
N |
|
MS |
Schilling & Overall, 2008 |
| 270 |
cathepsin L |
265-280 |
N |
|
MS |
Biniossek et al., 2011 |
| 270 |
cathepsin S |
265-280 |
N |
|
MS |
Biniossek et al., 2011 |
| 270 |
cathepsin B |
265-280 |
N |
|
MS |
Biniossek et al., 2011 |
| 270 |
matrix metallopeptidase-2 |
264-280 |
N |
|
MS |
Schilling & Overall, 2008 |
| 270 |
meprin beta subunit |
267-280 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 270 |
LAST peptidase (Limulus-type) |
267-280 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 270 |
LAST_MAM peptidase (Limulus-type) |
267-280 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 271 |
cathepsin B |
265-280 |
N |
|
MS |
Biniossek et al., 2011 |
| 272 |
LAST peptidase (Limulus-type) |
269-280 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 280 |
trypsin 1 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 283 |
LAST_MAM peptidase (Limulus-type) |
280-304 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 286 |
LAST_MAM peptidase (Limulus-type) |
283-304 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 286 |
cathepsin G |
280-304 |
N |
|
MS |
Schilling & Overall, 2008 |
| 293 |
LAST peptidase (Limulus-type) |
290-304 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 293 |
LAST_MAM peptidase (Limulus-type) |
290-304 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 304 |
trypsin 1 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 311 |
trypsin 1 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 316 |
HIV-1 retropepsin |
311-326 |
N |
|
MS |
Schilling & Overall, 2008 |
| 320 |
trypsin 1 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 326 |
trypsin 1 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 337 |
peptidyl-Lys metallopeptidase |
1-448 |
N |
|
MS |
|
| 339 |
trypsin 1 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 342 |
HIV-1 retropepsin |
341-352 |
N |
|
MS |
Schilling & Overall, 2008 |
| 343 |
cathepsin G |
341-352 |
N |
|
MS |
Schilling & Overall, 2008 |
| 351 |
peptidyl-Lys metallopeptidase |
1-448 |
N |
|
MS |
|
| 352 |
trypsin 1 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 355 |
elastase-2 |
352-370 |
N |
|
MS |
Schilling & Overall, 2008 |
| 356 |
cathepsin S |
353-370 |
N |
|
MS |
Biniossek et al., 2011 |
| 369 |
peptidyl-Lys metallopeptidase |
1-448 |
N |
|
MS |
|
| 370 |
trypsin 1 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 373 |
trypsin 1 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 377 |
matrix metallopeptidase-2 |
373-390 |
N |
|
MS |
Schilling & Overall, 2008 |
| 390 |
trypsin 1 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 394 |
trypsin 1 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 401 |
trypsin 1 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 422 |
trypsin 1 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
| 430 |
granzyme B (Homo sapiens-type) |
1-448 |
N |
|
MS |
Van Damme et al., 2010 |
| 430 |
trypsin 1 |
1-448 |
N |
|
MS |
Schilling & Overall, 2008 |
