Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 17 |
mitochondrial processing peptidase beta-subunit |
1-573 |
P |
|
CS |
Gevaert K et al., 2003 |
| 26 |
mitochondrial intermediate peptidase |
18-573 |
P |
release of a transit peptide |
MS |
Gevaert et al., 2003 |
| 31 |
lysyl endopeptidase (bacteria) |
1-573 |
N |
|
MS |
|
| 37 |
trypsin 1 |
27-573 |
N |
|
MS |
Schilling & Overall, 2008 |
| 48 |
meprin alpha subunit |
27-573 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 48 |
meprin beta subunit |
27-573 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 48 |
LAST_MAM peptidase (Limulus-type) |
45-58 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 49 |
caspase-1 |
27-573 |
N |
|
MS |
Agard et al., 2010 |
| 58 |
trypsin 1 |
27-573 |
N |
|
MS |
Schilling & Overall, 2008 |
| 60 |
trypsin 1 |
27-573 |
N |
|
MS |
Schilling & Overall, 2008 |
| 72 |
trypsin 1 |
27-573 |
N |
|
MS |
Schilling & Overall, 2008 |
| 125 |
lysyl endopeptidase (bacteria) |
1-573 |
N |
|
MS |
|
| 147 |
meprin alpha subunit |
144-157 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 147 |
LAST peptidase (Limulus-type) |
144-157 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 147 |
LAST_MAM peptidase (Limulus-type) |
144-157 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 149 |
meprin alpha subunit |
146-157 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 177 |
granzyme B (Homo sapiens-type) |
27-573 |
N |
|
MS |
Van Damme et al., 2010 |
| 180 |
trypsin 1 |
27-573 |
N |
|
MS |
Schilling & Overall, 2008 |
| 191 |
trypsin 1 |
27-573 |
N |
|
MS |
Schilling & Overall, 2008 |
| 205 |
trypsin 1 |
27-573 |
N |
|
MS |
Schilling & Overall, 2008 |
| 208 |
meprin alpha subunit |
27-573 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 208 |
meprin beta subunit |
27-573 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 208 |
LAST_MAM peptidase (Limulus-type) |
205-218 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 210 |
meprin beta subunit |
207-221 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 217 |
peptidyl-Lys metallopeptidase |
1-573 |
N |
|
MS |
|
| 218 |
trypsin 1 |
27-573 |
N |
|
MS |
Schilling & Overall, 2008 |
| 232 |
peptidyl-Lys metallopeptidase |
1-573 |
N |
|
MS |
|
| 240 |
meprin beta subunit |
237-250 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 256 |
meprin alpha subunit |
253-268 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 256 |
meprin beta subunit |
253-268 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 277 |
granzyme B (Homo sapiens-type) |
27-573 |
N |
|
MS |
Plasman et al., 2011 |
| 280 |
meprin beta subunit |
277-290 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 320 |
elastase-2 |
315-344 |
N |
|
MS |
Schilling & Overall, 2008 |
| 323 |
matrix metallopeptidase-2 |
316-344 |
N |
|
MS |
Schilling & Overall, 2008 |
| 325 |
HIV-1 retropepsin |
316-344 |
N |
|
MS |
Schilling & Overall, 2008 |
| 325 |
LAST peptidase (Limulus-type) |
322-344 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 326 |
LAST_MAM peptidase (Limulus-type) |
323-344 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 327 |
LAST_MAM peptidase (Limulus-type) |
324-344 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 329 |
HIV-1 retropepsin |
316-344 |
N |
|
MS |
Schilling & Overall, 2008 |
| 330 |
elastase-2 |
316-344 |
N |
|
MS |
Schilling & Overall, 2008 |
| 332 |
HIV-1 retropepsin |
316-344 |
N |
|
MS |
Schilling & Overall, 2008 |
| 332 |
elastase-2 |
316-344 |
N |
|
MS |
Schilling & Overall, 2008 |
| 335 |
cathepsin L |
315-344 |
N |
|
MS |
Biniossek et al., 2011 |
| 335 |
cathepsin S |
315-344 |
N |
|
MS |
Biniossek et al., 2011 |
| 335 |
cathepsin K |
316-344 |
N |
|
MS |
Schilling & Overall, 2008 |
| 335 |
glutamyl endopeptidase I |
316-344 |
N |
|
MS |
Schilling & Overall, 2008 |
| 341 |
matrix metallopeptidase-2 |
|
N |
|
MS |
Schilling & Overall, 2008 |
| 344 |
trypsin 1 |
27-573 |
N |
|
MS |
Schilling & Overall, 2008 |
| 346 |
cathepsin K |
345-359 |
N |
|
MS |
Schilling & Overall, 2008 |
| 347 |
matrix metallopeptidase-2 |
345-359 |
N |
|
MS |
Schilling & Overall, 2008 |
| 347 |
glutamyl endopeptidase I |
345-359 |
N |
|
MS |
Schilling & Overall, 2008 |
| 349 |
elastase-2 |
345-359 |
N |
|
MS |
Schilling & Overall, 2008 |
| 350 |
elastase-2 |
345-359 |
N |
|
MS |
Schilling & Overall, 2008 |
| 351 |
cathepsin K |
345-359 |
N |
|
MS |
Schilling & Overall, 2008 |
| 351 |
cathepsin G |
345-359 |
N |
|
MS |
Schilling & Overall, 2008 |
| 358 |
chymotrypsin A (cattle-type) |
1-573 |
N |
|
MS |
Schilling & Overall, 2008 |
| 359 |
trypsin 1 |
1-573 |
N |
|
MS |
Schilling & Overall, 2008 |
| 368 |
peptidyl-Lys metallopeptidase |
27-573 |
N |
|
MS |
|
| 378 |
LAST_MAM peptidase (Limulus-type) |
375-389 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 379 |
meprin alpha subunit |
376-389 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 381 |
cathepsin S |
371-389 |
N |
|
MS |
Biniossek et al., 2011 |
| 386 |
peptidyl-Lys metallopeptidase |
1-573 |
N |
|
MS |
|
| 407 |
cathepsin L |
406-417 |
N |
|
MS |
Biniossek et al., 2011 |
| 407 |
cathepsin S |
406-417 |
N |
|
MS |
Biniossek et al., 2011 |
| 433 |
meprin alpha subunit |
430-446 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 433 |
meprin beta subunit |
430-446 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 433 |
LAST_MAM peptidase (Limulus-type) |
430-446 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 451 |
meprin alpha subunit |
27-573 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 451 |
meprin beta subunit |
27-573 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 458 |
meprin alpha subunit |
27-573 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 458 |
meprin beta subunit |
27-573 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 480 |
peptidyl-Lys metallopeptidase |
1-573 |
N |
|
MS |
|
| 486 |
cathepsin K |
482-493 |
N |
|
MS |
Schilling & Overall, 2008 |
| 492 |
peptidyl-Lys metallopeptidase |
1-573 |
N |
|
MS |
|
| 493 |
trypsin 1 |
1-573 |
N |
|
MS |
Schilling & Overall, 2008 |
| 503 |
meprin alpha subunit |
27-573 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 503 |
meprin beta subunit |
27-573 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 503 |
LAST_MAM peptidase (Limulus-type) |
500-516 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 504 |
granzyme B (Homo sapiens-type) |
27-573 |
N |
|
MS |
Van Damme et al., 2010 |
| 507 |
cathepsin S |
494-516 |
N |
|
MS |
Biniossek et al., 2011 |
| 507 |
astacin |
504-516 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 508 |
meprin beta subunit |
27-573 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 537 |
matrix metallopeptidase-2 |
|
N |
|
MS |
Schilling & Overall, 2008 |
| 542 |
meprin alpha subunit |
539-554 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 551 |
trypsin 1 |
1-573 |
N |
|
MS |
Schilling & Overall, 2008 |
| 553 |
glutamyl endopeptidase I |
|
N |
|
MS |
Schilling & Overall, 2008 |
| 554 |
trypsin 1 |
1-573 |
N |
|
MS |
Schilling & Overall, 2008 |
