Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 5 |
trypsin 1 |
1-189 |
N |
|
MS |
Schilling & Overall, 2008 |
| 6 |
matrix metallopeptidase-2 |
1-16 |
N |
|
MS |
Schilling & Overall, 2008 |
| 10 |
meprin alpha subunit |
1-189 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 11 |
granzyme B (Homo sapiens-type) |
1-189 |
N |
|
MS |
Van Damme et al., 2010 |
| 12 |
granzyme B (Homo sapiens-type) |
1-189 |
N |
|
MS |
Van Damme et al., 2010 |
| 12 |
trypsin 1 |
1-189 |
N |
|
MS |
Schilling & Overall, 2008 |
| 16 |
glutamyl endopeptidase I |
1-189 |
N |
|
MS |
Schilling & Overall, 2008 |
| 18 |
glutamyl endopeptidase I |
12-27 |
N |
|
MS |
Schilling & Overall, 2008 |
| 27 |
trypsin 1 |
1-189 |
N |
|
MS |
Schilling & Overall, 2008 |
| 32 |
trypsin 1 |
1-189 |
N |
|
MS |
Schilling & Overall, 2008 |
| 36 |
cathepsin L |
33-46 |
N |
|
MS |
Biniossek et al., 2011 |
| 37 |
cathepsin B |
33-46 |
N |
|
MS |
Biniossek et al., 2011 |
| 37 |
matrix metallopeptidase-2 |
1-189 |
N |
|
MS |
Schilling & Overall, 2008 |
| 37 |
membrane-type matrix metallopeptidase-1 |
1-189 |
P |
|
|
Butler et al., 2008 |
| 37 |
astacin |
34-48 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 37 |
granzyme B (Homo sapiens-type) |
1-189 |
N |
|
MS |
Van Damme et al., 2010 |
| 41 |
trypsin 1 |
1-189 |
N |
|
MS |
Schilling & Overall, 2008 |
| 47 |
granzyme B (Homo sapiens-type) |
1-189 |
N |
|
MS |
Van Damme et al., 2010 |
| 48 |
trypsin 1 |
1-189 |
N |
|
MS |
Schilling & Overall, 2008 |
| 49 |
glutamyl endopeptidase I |
1-189 |
N |
|
MS |
Schilling & Overall, 2008 |
| 52 |
cathepsin L |
49-53 |
N |
|
MS |
Biniossek et al., 2011 |
| 52 |
cathepsin S |
49-63 |
N |
|
MS |
Biniossek et al., 2011 |
| 59 |
glutamyl endopeptidase I |
1-189 |
N |
|
MS |
Schilling & Overall, 2008 |
| 62 |
trypsin 1 |
1-189 |
N |
|
MS |
Schilling & Overall, 2008 |
| 63 |
trypsin 1 |
1-189 |
N |
|
MS |
Schilling & Overall, 2008 |
| 68 |
matrix metallopeptidase-2 |
63-89 |
N |
|
MS |
Schilling & Overall, 2008 |
| 70 |
elastase-2 |
63-89 |
N |
|
MS |
Schilling & Overall, 2008 |
| 76 |
matrix metallopeptidase-2 |
63-89 |
N |
|
MS |
Schilling & Overall, 2008 |
| 78 |
cathepsin L |
64-89 |
N |
|
MS |
Biniossek et al., 2011 |
| 81 |
matrix metallopeptidase-2 |
77-101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 81 |
meprin alpha subunit |
1-189 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 81 |
meprin beta subunit |
1-189 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 83 |
meprin alpha subunit |
1-189 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 83 |
meprin beta subunit |
1-189 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 89 |
trypsin 1 |
1-189 |
N |
|
MS |
Schilling & Overall, 2008 |
| 101 |
chymotrypsin A (cattle-type) |
1-189 |
N |
|
MS |
Schilling & Overall, 2008 |
| 107 |
LAST peptidase (Limulus-type) |
104-122 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 107 |
LAST_MAM peptidase (Limulus-type) |
104-122 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 111 |
matrix metallopeptidase-2 |
99-122 |
N |
|
MS |
Schilling & Overall, 2008 |
| 111 |
membrane-type matrix metallopeptidase-1 |
1-189 |
P |
|
|
Butler et al., 2008 |
| 112 |
HIV-1 retropepsin |
99-122 |
N |
|
MS |
Schilling & Overall, 2008 |
| 113 |
cathepsin S |
100-122 |
N |
|
MS |
Biniossek et al., 2011 |
| 114 |
HIV-1 retropepsin |
99-122 |
N |
|
MS |
Schilling & Overall, 2008 |
| 122 |
trypsin 1 |
1-189 |
N |
|
MS |
Schilling & Overall, 2008 |
| 131 |
peptidyl-Lys metallopeptidase |
1-189 |
N |
|
MS |
|
| 147 |
peptidyl-Lys metallopeptidase |
1-189 |
N |
|
MS |
|
| 149 |
caspase-6 |
1-189 |
P |
|
MU |
Giaime et al., 2009 |
| 164 |
HIV-1 retropepsin |
156-175 |
N |
|
MS |
Schilling & Overall, 2008 |
| 166 |
HIV-1 retropepsin |
156-175 |
N |
|
MS |
Schilling & Overall, 2008 |
| 171 |
matrix metallopeptidase-2 |
166-185 |
N |
|
MS |
Schilling & Overall, 2008 |
| 174 |
DJ-1 putative peptidase |
1-189 |
P |
|
MU |
Chen & Chin, 2010 |
| 175 |
trypsin 1 |
1-189 |
N |
|
MS |
Schilling & Overall, 2008 |
| 179 |
matrix metallopeptidase-2 |
176-189 |
N |
|
MS |
Schilling & Overall, 2008 |
| 180 |
matrix metallopeptidase-2 |
176-189 |
N |
|
MS |
Schilling & Overall, 2008 |
| 182 |
cathepsin L |
176-189 |
N |
|
MS |
Biniossek et al., 2011 |
| 182 |
trypsin 1 |
1-189 |
N |
|
MS |
Schilling & Overall, 2008 |
| 185 |
chymotrypsin A (cattle-type) |
1-189 |
N |
|
MS |
Schilling & Overall, 2008 |
