Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 1 |
methionyl aminopeptidase 2 |
1-335 |
P |
|
MS |
Warder et al., 2008 |
| 46 |
cathepsin S |
28-55 |
N |
|
MS |
Biniossek et al., 2011 |
| 46 |
cathepsin B |
28-55 |
N |
|
MS |
Biniossek et al., 2011 |
| 57 |
meprin beta subunit |
2-335 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 66 |
trypsin 1 |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 70 |
cathepsin S |
67-80 |
N |
|
MS |
Biniossek et al., 2011 |
| 70 |
astacin |
67-84 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 71 |
astacin |
68-80 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 80 |
trypsin 1 |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 81 |
caspase-6 |
2-335 |
P |
|
|
Klaiman et al., 2008 |
| 81 |
matrix metallopeptidase-2 |
79-94 |
N |
|
MS |
Schilling & Overall, 2008 |
| 84 |
matrix metallopeptidase-2 |
79-97 |
N |
|
MS |
Schilling & Overall, 2008 |
| 86 |
trypsin 1 |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 88 |
meprin beta subunit |
2-335 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 89 |
meprin alpha subunit |
2-335 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 89 |
meprin beta subunit |
2-335 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 91 |
meprin beta subunit |
2-335 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 92 |
meprin beta subunit |
2-335 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 93 |
meprin beta subunit |
2-335 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 94 |
HIV-1 retropepsin |
86-107 |
N |
|
MS |
Schilling & Overall, 2008 |
| 94 |
chymotrypsin A (cattle-type) |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 97 |
glutamyl endopeptidase I |
86-107 |
N |
|
MS |
Schilling & Overall, 2008 |
| 107 |
trypsin 1 |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 111 |
matrix metallopeptidase-2 |
106-138 |
N |
|
MS |
Schilling & Overall, 2008 |
| 122 |
cathepsin L |
108-132 |
N |
|
MS |
Biniossek et al., 2011 |
| 122 |
LAST peptidase (Limulus-type) |
119-139 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 124 |
LAST_MAM peptidase (Limulus-type) |
121-145 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 126 |
LAST_MAM peptidase (Limulus-type) |
123-145 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 127 |
HIV-1 retropepsin |
118-139 |
N |
|
MS |
Schilling & Overall, 2008 |
| 127 |
LAST peptidase (Limulus-type) |
124-139 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 127 |
LAST_MAM peptidase (Limulus-type) |
124-145 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 129 |
LAST_MAM peptidase (Limulus-type) |
126-145 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 130 |
cathepsin G |
118-139 |
N |
|
MS |
Schilling & Overall, 2008 |
| 131 |
matrix metallopeptidase-2 |
118-145 |
N |
|
MS |
Schilling & Overall, 2008 |
| 131 |
chymotrypsin A (cattle-type) |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 132 |
elastase-2 |
118-145 |
N |
|
MS |
Schilling & Overall, 2008 |
| 133 |
LAST_MAM peptidase (Limulus-type) |
130-145 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 138 |
glutamyl endopeptidase I |
118-145 |
N |
|
MS |
Schilling & Overall, 2008 |
| 139 |
trypsin 1 |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 145 |
trypsin 1 |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 150 |
elastase-2 |
146-162 |
N |
|
MS |
Schilling & Overall, 2008 |
| 151 |
matrix metallopeptidase-2 |
141-166 |
N |
|
MS |
Schilling & Overall, 2008 |
| 155 |
matrix metallopeptidase-2 |
141-166 |
N |
|
MS |
Schilling & Overall, 2008 |
| 159 |
matrix metallopeptidase-2 |
141-172 |
N |
|
MS |
Schilling & Overall, 2008 |
| 162 |
trypsin 1 |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 165 |
LAST_MAM peptidase (Limulus-type) |
162-186 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 166 |
caspase-6 |
2-335 |
P |
|
|
Klaiman et al., 2008 |
| 166 |
glutamyl endopeptidase I |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 172 |
glutamyl endopeptidase I |
162-186 |
N |
|
MS |
Schilling & Overall, 2008 |
| 173 |
HIV-1 retropepsin |
162-186 |
N |
|
MS |
Schilling & Overall, 2008 |
| 173 |
matrix metallopeptidase-2 |
162-186 |
N |
|
MS |
Schilling & Overall, 2008 |
| 175 |
meprin beta subunit |
171-186 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 175 |
LAST_MAM peptidase (Limulus-type) |
172-186 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 177 |
cathepsin B |
163-186 |
N |
|
MS |
Biniossek et al., 2011 |
| 180 |
matrix metallopeptidase-2 |
174-194 |
N |
|
MS |
Schilling & Overall, 2008 |
| 180 |
meprin beta subunit |
2-335 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 182 |
cathepsin L |
163-194 |
N |
|
MS |
Biniossek et al., 2011 |
| 183 |
meprin alpha subunit |
2-335 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 186 |
trypsin 1 |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 189 |
caspase-1 |
2-335 |
P |
|
MU |
Shao et al., 2007 |
| 194 |
trypsin 1 |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 195 |
chymotrypsin A (cattle-type) |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 196 |
chymotrypsin A (cattle-type) |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 198 |
glutamyl endopeptidase I |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 200 |
trypsin 1 |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 202 |
matrix metallopeptidase-2 |
200-215 |
N |
|
MS |
Schilling & Overall, 2008 |
| 204 |
meprin beta subunit |
201-215 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 204 |
LAST_MAM peptidase (Limulus-type) |
201-215 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 205 |
matrix metallopeptidase-2 |
203-228 |
N |
|
MS |
Schilling & Overall, 2008 |
| 210 |
matrix metallopeptidase-2 |
198-223 |
N |
|
MS |
Schilling & Overall, 2008 |
| 215 |
matrix metallopeptidase-2 |
212-228 |
N |
|
MS |
Schilling & Overall, 2008 |
| 215 |
trypsin 1 |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 219 |
trypsin 1 |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 223 |
glutamyl endopeptidase I |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 227 |
trypsin 1 |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 228 |
chymotrypsin A (cattle-type) |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 233 |
chymotrypsin A (cattle-type) |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 234 |
trypsin 1 |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 238 |
matrix metallopeptidase-2 |
234-248 |
N |
|
MS |
Schilling & Overall, 2008 |
| 238 |
meprin alpha subunit |
235-248 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 238 |
meprin beta subunit |
235-248 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 239 |
meprin alpha subunit |
236-248 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 248 |
trypsin 1 |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 254 |
trypsin 1 |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 263 |
trypsin 1 |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 271 |
trypsin 1 |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 275 |
cathepsin B |
272-291 |
N |
|
MS |
Biniossek et al., 2011 |
| 277 |
cathepsin L |
272-293 |
N |
|
MS |
Biniossek et al., 2011 |
| 280 |
cathepsin B |
272-291 |
N |
|
MS |
Biniossek et al., 2011 |
| 285 |
caspase-6 |
2-335 |
P |
|
|
Klaiman et al., 2008 |
| 285 |
matrix metallopeptidase-2 |
271-309 |
N |
|
MS |
Schilling & Overall, 2008 |
| 295 |
meprin alpha subunit |
2-335 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 295 |
LAST_MAM peptidase (Limulus-type) |
292-309 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 296 |
LAST_MAM peptidase (Limulus-type) |
293-309 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 296 |
glutamyl endopeptidase I |
271-309 |
N |
|
MS |
Schilling & Overall, 2008 |
| 298 |
astacin |
295-309 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 301 |
elastase-2 |
271-309 |
N |
|
MS |
Schilling & Overall, 2008 |
| 302 |
matrix metallopeptidase-2 |
271-309 |
N |
|
MS |
Schilling & Overall, 2008 |
| 309 |
trypsin 1 |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 323 |
trypsin 1 |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 326 |
matrix metallopeptidase-2 |
323-335 |
N |
|
MS |
Schilling & Overall, 2008 |
| 326 |
glutamyl endopeptidase I |
323-334 |
N |
|
MS |
Schilling & Overall, 2008 |
| 334 |
trypsin 1 |
2-335 |
N |
|
MS |
Schilling & Overall, 2008 |
