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Substrates for peptidase S01.224: hepsin

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Pharma

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate Uniprot Residue range Cleavage Site Cleavage type Evidence P4 P3 P2 P1 P1' P2' P3' P4' Reference CutDB MERNUM
14-3-3 protein theta P27348 138-151 R+KQTIDNSQGAYQE N MS - - - Arg Lys Gln Thr Ile Barré et al., 2014
26S protease regulatory subunit 10B P62333 297-306 R+KIHIDLPNE N MS - - - Arg Lys Ile His Ile Barré et al., 2014
40S ribosomal protein S24 P62847 110-133 peptide-Arg118+Gly-peptide N MS Lys Lys Val Arg Gly Thr Ala Lys Barré et al., 2014
40S ribosomal protein S3 P23396 2-20 AVQISKKR+KFVADGIFKAE N MS Ser Lys Lys Arg Lys Phe Val Ala Barré et al., 2014
40S ribosomal protein S3a P61247 33-68 peptide-Arg51+Thr-peptide N MS Leu Val Thr Arg Thr Gln Gly Thr Barré et al., 2014
60S ribosomal protein L11 P62913 162-178 AMR+WFQQKYDGIILPGK N MS - Ala Met Arg Trp Phe Gln Gln Barré et al., 2014
60S ribosomal protein L12 P30050 92-108 R+KKQKNIKHSGNITFDE N MS - - - Arg Lys Lys Gln Lys Barré et al., 2014
Abz-Arg-Ala-Ala-Arg-Val-Val-Gly-Gly-Tyr(NO2) Abz-Arg-Ala-Ala-Arg+Val-Val-Gly-Gly-Tyr(NO2) S Arg Ala Ala Arg Val Val Gly Gly Beliveau et al., 2009
Abz-Arg-Arg-Ala-Arg-Val-Val-Gly-Gly-Tyr(NO2) Abz-Arg-Arg-Ala-Arg+Val-Val-Gly-Gly-Tyr(NO2) S Arg Arg Ala Arg Val Val Gly Gly Beliveau et al., 2009
Abz-Arg-Gln-Ala-Arg-Ala-Val-Gly-Gly-Tyr(NO2) Abz-Arg-Gln-Ala-Arg+Ala-Val-Gly-Gly-Tyr(NO2) S Arg Gln Ala Arg Ala Val Gly Gly Beliveau et al., 2009
Abz-Arg-Gln-Ala-Arg-Tyr-Val-Gly-Gly-Tyr(NO2) Abz-Arg-Gln-Ala-Arg+Tyr-Val-Gly-Gly-Tyr(NO2) S Arg Gln Ala Arg Tyr Val Gly Gly Beliveau et al., 2009
Abz-Arg-Gln-Ala-Arg-Val-Val-Gly-Gly-Tyr(NO2) Abz-Arg-Gln-Ala-Arg-Val-Val-Gly-Gly-Tyr(NO2) S Arg Gln Ala Arg Val Val Gly Gly Beliveau et al., 2009
Abz-Arg-Gln-Arg-Arg-Val-Val-Gly-Gly-Tyr(NO2) Abz-Arg-Gln-Arg-Arg+Val-Val-Gly-Gly-Tyr(NO2) S Arg Gln Arg Arg Val Val Gly Gly Beliveau et al., 2009
Abz-Arg-Gln-Leu-Arg-Val-Val-Gly-Gly-Tyr(NO2) Abz-Arg-Gln-Leu-Arg+Val-Val-Gly-Gly-Tyr(NO2) S Arg Gln Leu Arg Val Val Gly Gly Beliveau et al., 2009
Abz-Arg-Gln-Tyr-Arg-Val-Val-Gly-Gly-Tyr(NO2) Abz-Arg-Gln-Tyr-Arg+Val-Val-Gly-Gly-Tyr(NO2) S Arg Gln Tyr Arg Val Val Gly Gly Beliveau et al., 2009
Abz-Arg-Leu-Ala-Arg-Val-Val-Gly-Gly-Tyr(NO2) Abz-Arg-Leu-Ala-Arg+Val-Val-Gly-Gly-Tyr(NO2) S Arg Leu Ala Arg Val Val Gly Gly Beliveau et al., 2009
Ac-Lys-Gln-Leu-Arg-NHMec Ac-Lys-Gln-Leu-Arg+NHMec S Lys Gln Leu Arg AMC - - - Herter et al., 2005
Actin, aortic smooth muscle P62736 28-59 peptide-Arg41+His-peptide N MS Gly Arg Pro Arg His Gln Gly Val Barré et al., 2014
Alpha-endosulfine Q6VUC6 103-121 LPQR+KSSLVTSKLAGGQVE N MS Leu Pro Gln Arg Lys Ser Ser Leu Barré et al., 2014
Angiomotin Q4VCS5 533-546 TR+KTISQLFAKNKE N MS - - Thr Arg Lys Thr Ile Ser Barré et al., 2014
ATP synthase subunit alpha, mitochondrial Q96IR6 484-508 peptide-Arg493+Gly-peptide N MS Ala Gly Val Arg Gly Tyr Leu Asp Barré et al., 2014
Bcl-2-associated transcription factor 1 Q9NYF8 271-314 peptide-Arg299+Ser-peptide N MS Pro Ser Arg Arg Ser Pro Ala Lys Barré et al., 2014
Bloom syndrome protein P54132 99-117 TQR+GGSKSLLPDFLQTPKE N MS - Thr Gln Arg Gly Gly Ser Lys Barré et al., 2014
Bz-Ile-Glu-Phe-Ser-Arg-NHPhNO2 Bz-Ile-Glu-Phe-Ser-Arg+NHPhNO2 S Glu Phe Ser Arg NAN - - - Kurachi & Yakamoto, 2004
Bz-Leu-Ser-Arg-NHPhNO2 Bz-Leu-Ser-Arg+NHPhNO2 S Bz Leu Ser Arg NAN - - - Kurachi & Yakamoto, 2004
Bz-Phe-Val-Arg-NHPhNO2 Bz-Phe-Val-Arg+NHPhNO2 S Bz Phe Val Arg NAN - - - Kurachi & Yakamoto, 2004
Calcium signal-modulating cyclophilin ligand P49069 162-177 AMKLR+KQLISEKPSQE N MS Met Lys Leu Arg Lys Gln Leu Ile Barré et al., 2014
cDNA FLJ45706 fis, clone FEBRA2028457, highly similar to Nucleolin Q6ZS99 285-301 AR+TLLAKNLPYKVTQDE N MS - - Ala Arg Thr Leu Leu Ala Barré et al., 2014
Cellular nucleic acid-binding protein P20694 73-93 ACYNCGR+GGHIAKDCKEPKRE N MS Asn Cys Gly Arg Gly Gly His Ile Barré et al., 2014
Clathrin heavy chain 1 Q00610 963-981 SNPYR+RPLIDQVVQTALSE N MS Asn Pro Tyr Arg Arg Pro Leu Ile Barré et al., 2014
Clathrin heavy chain 1 Q00610 213-232 STLFCFAVR+GQAGGKLHIIE N MS Phe Ala Val Arg Gly Gln Ala Gly Barré et al., 2014
Cleavage and polyadenylation specificity factor subunit 6 Q16630 465-481 R+CKVLISSLQDCLHGIE N MS - - - Arg Cys Lys Val Leu Barré et al., 2014
coagulation factor IX P00740 47-461 peptide-Arg226+Val-peptide P Asp Phe Thr Arg Val Val Gly Gly Herter et al., 2005 20445
coagulation factor VII P08709 21-466 peptide-Arg212+Ile-peptide P Pro Gln Gly Arg Ile Val Gly Gly Kazama et al., 1995 3345
coagulation factor XII P00748 20-615 peptide-Arg372+Val-peptide P Ser Met Thr Arg Val Val Gly Gly Herter et al., 2005 20444
Coiled-coil domain-containing protein 124 Q96CT7 188-203 NPNMR+LSQLKQLLKKE N MS Pro Asn Met Arg Leu Ser Gln Leu Barré et al., 2014
Coiled-coil domain-containing protein 86 Q9H6F5 181-203 peptide-Arg190+Gly-peptide N MS Gly Ser Pro Arg Gly Gln His Glu Barré et al., 2014
Cytochrome c oxidase subunit 6C P09669 65-75 MR+KAGIFQSVK N MS - - Met Arg Lys Ala Gly Ile Barré et al., 2014
D-3-phosphoglycerate dehydrogenase O43175 87-108 peptide-Arg90+Lys-peptide N MS Ala Ala Thr Arg Lys Gly Ile Leu Barré et al., 2014
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial P10515 314-367 peptide-Arg354+Val-peptide N MS Pro Lys Gly Arg Val Phe Val Ser Barré et al., 2014
DNA fragmentation factor subunit alpha O00273 161-184 peptide-Arg170+Leu-peptide N MS Thr Val Gln Arg Leu Gln His Thr Barré et al., 2014
Dynactin subunit 2 Q13561 329-341 LVQR+LVTIKQLHE N MS Leu Val Gln Arg Leu Val Thr Ile Barré et al., 2014
Eukaryotic translation initiation factor 3 subunit F O00303 112-130 GAAR+VIGTLLGTVDKHSVE N MS Gly Ala Ala Arg Val Ile Gly Thr Barré et al., 2014
Eukaryotic translation initiation factor 3 subunit G O75821 164-181 HWTTR+CPYKDTLGPMQKE N MS Trp Thr Thr Arg Cys Pro Tyr Lys Barré et al., 2014
Eukaryotic translation initiation factor 5A-2 Q9GZV4 12-42 peptide-Arg26+Lys-peptide N MS Ser Ala Leu Arg Lys Asn Gly Phe Barré et al., 2014
Filamin-A P21333 2143-2161 SITRRR+RAPSVANVGSHCD N MS Thr Arg Arg Arg Arg Ala Pro Ser Barré et al., 2014
Fructose-bisphosphate aldolase C P09972 19-35 IALR+IVAPGKGILAADE N MS Ile Ala Leu Arg Ile Val Ala Pro Barré et al., 2014
Glucosidase 2 subunit beta P14314 17-33 VKRPR+GVSLTNHHFYDE N MS Lys Arg Pro Arg Gly Val Ser Leu Barré et al., 2014
Glutaryl-CoA dehydrogenase, mitochondrial Q92947 32-47 KGGR+TQSQLAKSSRPE N MS Lys Gly Gly Arg Thr Gln Ser Gln Barré et al., 2014
Glutathione reductase, mitochondrial P00390 169-185 IIR+GHAAFTSDPKPTIE N MS - Ile Ile Arg Gly His Ala Ala Barré et al., 2014
Glyceraldehyde-3-phosphate dehydrogenase P04406 318-335 FGYSNR+VVDLMAHMASKE N MS Tyr Ser Asn Arg Val Val Asp Leu Barré et al., 2014
GMP synthase [glutamine-hydrolyzing] P49915 336-350 KR+KIIGDTFVKIANE N MS - - Lys Arg Lys Ile Ile Gly Barré et al., 2014
Growth factor receptor-bound protein 2 P62993 17-31 LSFKR+GDILKVLNEE N MS Ser Phe Lys Arg Gly Asp Ile Leu Barré et al., 2014
Heat shock 70 kDa protein 1A/1B P08107 49-69 R+LIGDAAKNQVALNPQNTVFD N MS - - - Arg Leu Ile Gly Asp Barré et al., 2014
hepatocyte growth factor P14210 32-728 peptide-Arg494+Val-peptide P Lys Gln Leu Arg Val Val Asn Gly Herter et al., 2005 20442
hepsin precursor P05981 1-417 peptide-Arg162+Ile-peptide P Pro Val Asp Arg Ile Val Gly Gly Herter et al., 2005 20447
Heterogeneous nuclear ribonucleoprotein A/B Q99729 309-332 peptide-Arg322+Gly-peptide N MS Ser Gln Arg Arg Gly Gly His Gln Barré et al., 2014
Heterogeneous nuclear ribonucleoprotein K P61978 191-204 R+VVLIGGKPDRVVE N MS - - - Arg Val Val Leu Ile Barré et al., 2014
Heterogeneous nuclear ribonucleoprotein M P52272 707-721 RACR+MMNGMKLSGRE N MS Arg Ala Cys Arg Met Met Asn Gly Barré et al., 2014
Heterogeneous nuclear ribonucleoproteins C1/C2 Q86U45 135-199 peptide-Arg180+Gly-peptide N MS Ser Gly Gln Arg Gly Ser Ser Lys Barré et al., 2014
Histone H2A type 1-B/E P04908 94-122 peptide-Gly106+Gly-peptide N MS Ile Ala Gln Gly Gly Val Leu Pro Barré et al., 2014
Histone H2A type 1-B/E P04908 94-122 peptide-Arg100+Val-peptide N MS Leu Leu Gly Arg Val Thr Ile Ala Barré et al., 2014
Homeobox protein cut-like 1 Q9UEV5 49-63 LR+KQVAPLLKSFQGE N MS - - Leu Arg Lys Gln Val Ala Barré et al., 2014
Importin subunit beta-1 Q14974 850-868 GRR+SKTNKAKTLATWATKE N MS - Gly Arg Arg Ser Lys Thr Asn Barré et al., 2014
Interleukin enhancer-binding factor 3 Q9UNA2 12-27 R+HVMAKHSSVYPTQEE N MS - - - Arg His Val Met Ala Barré et al., 2014
Intraflagellar transport protein 140 homolog Q96RY7 166-178 LVQL+AKAAVSGDE N MS Leu Val Gln Leu Ala Lys Ala Ala Barré et al., 2014
L-lactate dehydrogenase A chain Q9UDE8 314-329 AR+LKKSADTLWGIQKE N MS - - Ala Arg Leu Lys Lys Ser Barré et al., 2014
La-related protein 1 Q6PKG0 147-181 peptide-Arg162+Lys-peptide N MS Pro Lys Gln Arg Lys Gly Ser Lys Barré et al., 2014
Lamin-B1 P20700 89-99 AR+RALDDTARE N MS - - Ala Arg Arg Ala Leu Asp Barré et al., 2014
laminin subunit beta-3 Q61087 18-1168 peptide-Arg245+Leu-peptide P Ser Gln Leu Arg Leu Gln Gly Ser Tripathi et al., 2008 22134
Lysine-rich nucleolar protein 1 Q1ED39 237-254 SSPR+KGSKKKPVKVEAPE N MS Ser Ser Pro Arg Lys Gly Ser Lys Barré et al., 2014
Lysine-rich nucleolar protein 1 Q1ED39 95-108 KSPSLR+KQVFGHLE N MS Pro Ser Leu Arg Lys Gln Val Phe Barré et al., 2014
MAP3K7 C-terminal-like protein P57077 103-117 WMLTL+KPKSITVPVE N MS Met Leu Thr Leu Lys Pro Lys Ser Barré et al., 2014
Mitochondrial import inner membrane translocase subunit TIM44 O43615 45-70 peptide-Arg56+Lys-peptide N MS Ser Gly Asn Arg Lys Gly Phe Leu Barré et al., 2014
Myelin expression factor 2 Q9NUQ1 29-39 PR+REPHPAEAE N MS - - Pro Arg Arg Glu Pro His Barré et al., 2014
Nuclease-sensitive element-binding protein 1 P67809 271-302 peptide-Arg290+Arg-peptide N MS Tyr Arg Arg Arg Arg Pro Glu Asn Barré et al., 2014
Nuclease-sensitive element-binding protein 1 P67809 271-302 peptide-Arg289+Arg-peptide N MS Asn Tyr Arg Arg Arg Arg Pro Glu Barré et al., 2014
Nucleolar protein 56 O00567 550-574 peptide-Arg560+Lys-peptide N MS Lys Lys Lys Arg Lys Phe Ser Lys Barré et al., 2014
Nucleoprotein TPR P12270 2132-2151 R+TVPSTPTLVVPHRTDGFAE N MS - - - Arg Thr Val Pro Ser Barré et al., 2014
Peroxiredoxin-1 Q06830 156-171 TLR+LVQAFQFTDKHGE N MS - Thr Leu Arg Leu Val Gln Ala Barré et al., 2014
Phenylalanine--tRNA ligase beta subunit Q9NSD9 551-573 peptide-Arg554+Gly-peptide N MS Ile Phe Ala Arg Gly Gln Ser Val Barré et al., 2014
Plasminogen activator inhibitor 1 RNA-binding protein Q9Y367 215-230 KR+GGSGSHNWGTVKDE N MS - - Lys Arg Gly Gly Ser Gly Barré et al., 2014
Plasminogen activator inhibitor 1 RNA-binding protein Q9Y367 78-94 R+KNPLPPSVGVVDKKEE N MS - - - Arg Lys Asn Pro Leu Barré et al., 2014
Poly [ADP-ribose] polymerase 1 P09874 492-514 peptide-Arg496+Gly-peptide N MS Val Ala Pro Arg Gly Lys Ser Gly Barré et al., 2014
Poly [ADP-ribose] polymerase 1 P09874 492-513 peptide-Arg496+Gly-peptide N MS Val Ala Pro Arg Gly Lys Ser Gly Barré et al., 2014
Pre-mRNA-processing factor 40 homolog A O75400 426-436 PR+YSALAKLSE N MS - - Pro Arg Tyr Ser Ala Leu Barré et al., 2014
Probable ATP-dependent RNA helicase DDX5 P17844 260-272 PQIR+KIVDQIRPD N MS Pro Gln Ile Arg Lys Ile Val Asp Barré et al., 2014
Profilin-1 P07737 56-76 R+SSFYVNGLTLGGQKCSVIRD N MS - - - Arg Ser Ser Phe Tyr Barré et al., 2014
Protein DJ-1 Q99497 97-116 NR+KGLIAAICAGPTALLAHE N MS - - Asn Arg Lys Gly Leu Ile Barré et al., 2014
Protein LSM12 homolog Q3MHD2 175-190 SQKILQR+SQAQQPQKE N MS Ile Leu Gln Arg Ser Gln Ala Gln Barré et al., 2014
Protein scribble homolog Q14160 1110-1132 peptide-Arg1120+Gly-peptide N MS Gly Gly Ala Arg Gly His Ala Gly Barré et al., 2014
Putative heat shock protein HSP 90-alpha A2 Q14568 179-196 RMGR+GTKVILHLKEDQTE N MS Arg Met Gly Arg Gly Thr Lys Val Barré et al., 2014
Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 O43143 2-11 SKR+HRLDLGE N MS - Ser Lys Arg His Arg Leu Asp Barré et al., 2014
RNA-binding protein 10 P98175 145-158 IR+GQLQSHGVQARE N MS - - Ile Arg Gly Gln Leu Gln Barré et al., 2014
RNA-binding protein 25 Q9UEQ5 244-262 IFR+RFPVAPLIPYPLITKE N MS - Ile Phe Arg Arg Phe Pro Val Barré et al., 2014
Serum albumin Q56G89 166-177 IAR+RHPYFYAPE N MS - Ile Ala Arg Arg His Pro Tyr Barré et al., 2014
Small acidic protein O00193 90-106 SKLSGRYR+RHCGLGFSE N MS Gly Arg Tyr Arg Arg His Cys Gly Barré et al., 2014
Small glutamine-rich tetratricopeptide repeat-containing protein alpha O43765 294-313 QLRSQIR+SRTPSASNDDQQE N MS Ser Gln Ile Arg Ser Arg Thr Pro Barré et al., 2014
Small ubiquitin-related modifier 1 P63165 68-79 GQR+IADNHTPKE N MS - Gly Gln Arg Ile Ala Asp Asn Barré et al., 2014
Splicing factor 45 Q96I25 269-286 KTSKR+GGKIIVGDATEKD N MS Thr Ser Lys Arg Gly Gly Lys Ile Barré et al., 2014
Splicing factor, arginine/serine-rich 19 Q9H7N4 715-729 PR+GPSPAPASSPKRE N MS - - Pro Arg Gly Pro Ser Pro Barré et al., 2014
STE20-like serine/threonine-protein kinase Q9NQL0 842-857 QR+AQQQLNSKLQQQRE N MS - - Gln Arg Ala Gln Gln Gln Barré et al., 2014
Stress-induced-phosphoprotein 1 P31948 35-59 peptide-Arg44+Ser-peptide N MS Tyr Ser Asn Arg Ser Ala Ala Tyr Barré et al., 2014
T-complex protein 1 subunit delta P50991 525-539 TVR+SILKIDDVVNTR N MS - Thr Val Arg Ser Ile Leu Lys Barré et al., 2014
T-complex protein 1 subunit eta Q99832 392-413 peptide-Arg397+Arg-peptide N MS Met Ile Val Arg Arg Ala Ile Lys Barré et al., 2014
Trans-2-enoyl-CoA reductase, mitochondrial Q9BV79 54-64 PA+KVVELKNLE N MS - - Pro Ala Lys Val Val Glu Barré et al., 2014
Tropomyosin alpha-4 chain Q9UCS3 9-23 AVKR+KIQALQQQADE N MS Ala Val Lys Arg Lys Ile Gln Ala Barré et al., 2014
urokinase-type plasminogen activator P00749 21-431 peptide-Lys178+Ile-peptide P Pro Arg Phe Lys Ile Ile Gly Gly Moran et al., 2006 19122
Vesicle-associated membrane protein 3 Q15836 2-24 peptide-Arg13+Arg-peptide N MS Gly Ser Asn Arg Arg Leu Gln Gln Barré et al., 2014
Vimentin Q8N850 157-172 LRR+QVDQLTNDKARVE N MS - Leu Arg Arg Gln Val Asp Gln Barré et al., 2014

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