Chart Builder documentation
Summary
Introduction
The EMDB Chart Builder (https://emdb-empiar.org/statistics/builder) is a web-based application that provides users with the ability to create customizable, dynamic, and informative charts that help analyse the holdings and trends of the EMDB and EMPIAR archives, powered by the EMDB search engine. The underlying data is based on the metadata of all EMDB entries, enriched by the EMICSS resource (https://emdb-empiar.org/emicss). This tool allows users to define specific search terms, enabling the extraction of data subsets that are directly relevant to their analysis objectives. By organising the extracted data into meaningful categories, users can generate insightful charts to visualise and analyse the results. The Chart Builder can be accessed at https://ebi.ac.uk/emdb/statistics/builder.This page presents a user-friendly interface specifically designed to facilitate the creation of customised charts using both EMDB and EMPIAR data.
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Multiple data series definition -
Plot charts from categorical variables -
Multiple stack modes for the charts. -
Display the data in linear or logarithmic scales.
Use of the Chart Builder requires some knowledge of the EMDB search engine. Therefore you might find it useful to read the search system documentation and the list of search fields. These resources provide valuable insights into the various search functionalities and parameters that can be employed to refine your data extraction.
The Chart builder now allows you to open and customise most of the charts available at /emdb/statistics. If you see an edit icon, simply click on it to open the corresponding chart in the Chart builder. This feature provides you with the flexibility to modify and personalise the chart according to your specific requirements, enabling you to derive deeper insights from the data visualisation.
Fields
Archive
Select if your chart is going to be using data about EMDB or EMPIAR. (Note: the amount of available metadata is currently much greater for EMDB than for EMPIAR.)
Chart type
The Chart Builder currently supports four types of charts:
- Line: This chart type is best suited for visualising trends and changes over time. It can be used to
illustrate how properties of one or more subsets of entries evolve over time.Column: The column chart
is ideal for comparing different categories or groups.
Figure 2: Line chart example. - Bar: The bar chart is ideal for comparing different categories or groups. It provides a visual
representation of the distribution and relative magnitudes of specific entries within each category.
Figure 3: Bar chart example. - Pie: The pie chart is a useful tool for illustrating proportions and percentages. It provides a concise
visual representation of the distribution of a selected subset of entries among various categories.
Pie charts can only have one data series, and the data categories are defined by the Data X parameter.
Figure 4: Pie chart example. - Area: Similar to a line chart, the area chart emphasises the cumulative size of subsets of entries over time.
Figure 5: Area chart example.
Global filter
The optional global filter in the Chart Builder allows you to specify a search term that will be applied to the selected entries for the entire chart. This feature ensures that the resulting plot focuses on the specific data subset defined by the search term. For instance, suppose you wish to create a plot showcasing the number of entries related to Sars-Cov-2 per EM method. In that case, you can simply change one of the previous examples and set the following query within the global filter: `natural_source_ncbi_code:"2697049"`. This query narrows down the data to entries associated with the Sars-Cov-2 NCBI code, enabling the creation of a customised plot that visualises the desired information accurately.
Data X
The Data X field determines the data that will be displayed on the X-axis or as categories in a pie chart. Within this field, there are three types of data X definitions:
Year:
The X-axis values in the chart correspond to the release year of the entries. By modifying the Data X settings, you can refine the previously created SARS-CoV-2 chart to display data specifically from the year 2019 and beyond.
Experimental metadata:
When selecting the option of "Experimental metadata" for the Data X field, you have the ability to choose one of the enumeration categories. The chosen category's elements will be displayed on the X-axis of the chart. For instance, you can set the experimental metadata category as an EM method to showcase the number of Sars-Cov-2 entries per each type of experiment.
Custom queries:
You can define custom queries to specify a set of criteria that will be displayed on the x-axis of the chart. This allows you to customise the chart further according to your specific requirements. For example, you can modify the previous chart to include only the columns related to single-particle, subtomogram averaging, and tomography by using custom queries.
Custom queries are particularly important when creating pie charts since they can only have a single data series, and the slices of the pie are defined by the elements in the custom queries. We can modify the previous chart to be displayed as a pie chart. This allows for a concise visual representation of the distribution of the selected subset of entries among each experiment method.
Data Y:
The Data Y field is used to set what information is going to be displayed along the Y-axis of your chart. For example, you can choose to display the number of entries, publications, or resolution on the Y-axis, depending on the nature of your analysis and the insights you seek to derive from the chart.
Data series:
This field defines the data series that will be displayed in your chart. A data series consists of three pieces of information: operator, query, and label. The available operators depend on the selection made in the Data Y field. If Data Y is a numeric variable, the operator can be average, minimum, maximum, or sum. On the other hand, if Data Y is a categorical variable, the operator can be the count of unique values or cumulative values. Additionally, you can apply an optional filter query for each data series. This is useful when comparing multiple classes of information, as demonstrated in the example below.
Attributes
There are seven attributes that can be used to print, download, export and change the chart visualisation:
- Change how area and columns are displayed. There are three options: unstacked, stacked and stacked percent.
- Select to display the data series in a logarithmic or linear scale.
- Share the current chart. The resulting URL will be copied into the clipboard.
- Print the current chart.
- Download the chart image or data table.
- Full Screen mode.
- Extra options.
Quick links
Recent Entries
(Show all)Cryo-EM structure of Shigella flexneri LptDE dimer: closed-state unbound and open-state bound by Oekolampad phage RBP
Tomogram of yeast cell overexpressing Ldm1, treated with alpha-factor
Ancestral Fibrobacteres-Chlorobi-Bacteroidetes Group Chaperonin (AFCB) Double-Ring in Open Conformation
3D Reconstruction of Ancestral Group II Chaperonin (ACII) Double-Ring in Open Conformation
3D Reconstruction of Ancestral Group I Chaperonin (ACI) Single-Ring
3D Reconstruction of Ancestral Group II Chaperonin (ACII) Single-Ring in Open Conformation
Hexahistidine-tagged tobacco mosaic virus coat protein 5-layer disk
3D Reconstruction of Ancestral Group II Chaperonin (ACII) Single-Ring in Closed Conformation
Ancestral Group II Chaperonin (ACII) Double-Ring in Closed Conformation
Herpes simplex virus 2 delta28-73 glycoprotein C ectodomain in complex with C3b
Hexahistidine-tagged tobacco mosaic virus coat protein 6-layer disk
Cryo-EM structure of Sup35NM S17R fibril formed at 4 degrees (S17R4N)
Cryo-EM structure of Sup35NM S17R fibril formed at 37 degrees (S17R37N)
Cryo-EM structure of Sup35NM S17R fibril formed at 37 degrees (S17R37C)
Structure of the canthaxanthin mutant PSI-5VCPI supercomplex in Nannochloropsis oceanica
Cryo-EM structure of Sup35NM S17R fibril formed at 4 degrees (S17R4C)
Structure of the wild-type PSI-5VCPI supercomplex in Nannochloropsis oceanica
Structure of the canthaxanthin mutant PSI-3VCPI supercomplex in Nannochloropsis oceanica
Structure of the astaxanthin mutant PSI-7VCPI supercomplex in Nannochloropsis oceanica
Structure of the astaxanthin mutant PSI-9VCPI supercomplex in Nannochloropsis oceanica
Structure of the canthaxanthin mutant PSI-9VCPI supercomplex in Nannochloropsis oceanica
Structure of the astaxanthin mutant PSI-5VCPI supercomplex in Nannochloropsis oceanica
Structure of the canthaxanthin mutant PSI-4VCPI supercomplex in Nannochloropsis oceanica
Structure of SARS-CoV-2 EG.5.1 Variant Spike protein complexed with antibody XGi-203
The local refined map of SARS-CoV-2 EG.5.1 Variant Spike protein complexed with antibody XGi-183
Focused refinement up-RBD1 of SARS-CoV-2 EG.5.1 Variant Spike protein complexed with antibody XGi-203
Focused refinement up-RBD2 of SARS-CoV-2 EG.5.1 Variant Spike protein complexed with antibody XGi-203
Raw consensus map of SARS-CoV-2 EG.5.1 Variant Spike protein complexed with antibody XGi-203
The local refined map of SARS-CoV-2 EG.5.1 Variant Spike protein complexed with antibody XGi-203
The local refined map of SARS-CoV-2 EG.5.1 Variant Spike protein complexed with antibody XGi-171
Eleven polymer Msp1 from S.cerevisiae (with a catalytic dead mutaion) in complex with an unknown peptide substrate
The local refined map of SARS-CoV-2 EG.5.1 Variant Spike protein complexed with antibody XGi-198
Structure of SARS-CoV-2 EG.5.1 Variant Spike protein complexed with antibody XGi-171
Focused refinement trimer2 of SARS-CoV-2 EG.5.1 Variant Spike protein complexed with antibody XGi-198
Focused refinement trimer1 of SARS-CoV-2 EG.5.1 Variant Spike protein complexed with antibody XGi-198
Raw consensus map of SARS-CoV-2 EG.5.1 Variant Spike protein complexed with antibody XGi-198
Structure of SARS-CoV-2 EG.5.1 Variant Spike protein complexed with antibody XGi-198
Cryo-EM structure of Shigella flexneri LptDE in complex with RTP45 superinfection exclusion protein from RTP bacteriophage and endogenous LptM
Local refined cryo-EM map focusing on ZAK-RACK1 of the stalled 80S
Cryo-EM structure of the open state of Shigella flexneri LptDE bound by the RBP of Oekolampad phage
The cryo-EM structure of porcine serum MGAM bound with Acarviosyl-maltotriose.
Cryo-EM structure of rabbit TRPM3 in apo resting state at 18 degrees Celsius
Cryo-EM Structure of HIV-1 BG505DS-SOSIP.664 Env Trimer Bound to DFPH-a.01_10R59P_LC Fab
Focused reconstruction of protomer B of the PrPfr hybrid state of the Pseudomonas aeruginosa bacteriophytochrome / PaBphP
Consensus reconstruction of the PrPfr hybrid state of the Pseudomonas aeruginosa bacteriophytochrome / PaBphP
Focus map on protomer A of the PrPfr hybrid state of the Pseudomonas aeruginosa bacteriophytochrome / PaBphP
Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA
Late-stage 48S Initiation Complex with eIF3 (LS48S-eIF3 IC) guided by the trans-RNA
Structure of RACK1 bound to the C-terminus of SERBP1 and the RIH region of ZAK
Cryo-EM structure of human SV2A in complex with UCBJ and UCB1244283
receptor focused refinement of the human delta opioid complex with mini-Gi, agonist DADLE and allosteric modulator MIPS3614
receptor focused refinement of human delta opioid receptor complex with mini-Gi and DADLE
receptor focused refinement of human delta opioid receptor complex with mini-Gi, agonist DADLE and allosteric modulator MIPS3983
Chlorella virus hyaluronan synthase bound to a proofreading UDP-GlcA
Chlorella virus hyaluronan synthase bound to an inserted UDP-GlcA
Cryo-EM structure of human SV2A in complex with Levetiracetam and UCB1244283
conensus map of the human delta opioid complex with mini-Gi, agonist DADLE and allosteric modulator BMS-986187
Human delta opioid receptor complex with mini-Gi and agonist DADLE
Human delta opioid receptor complex with mini-Gi and agonist DADLE and allosteric modulator MIPS3614
Human delta opioid receptor complex with mini-Gi and agonist DADLE and allosteric modulator MIPS3983
receptor focused refinement of human delta opioid complex with mini-Gi, agonist DADLE and allosteric modulator BMS-986187
CryoEM structure of G protein-gated inwardly rectifying potassium channel GIRK2 R92F (State 2)
CryoEM structure of G protein-gated inwardly rectifying potassium channel 2 R92F (State 1)
Translational activators Aep1, Aep2 and Atp25 in complex with mRNA and the yeast mitochondrial ribosome (composite map SSU and Aep1-Aep2-Atp25 complex)
Cryo-EM structure of G protein-gated inwardly rectifying potassium channel 2 R92Q (State 2)
Translational activators Aep1, Aep2 and Atp25 in complex with mRNA and the yeast mitochondrial ribosome (large ribosomal subunit)
Translational activators Aep1, Aep2 and Atp25 in complex with mRNA and the yeast mitochondrial ribosome
Cryo-EM structure of G protein-gated inwardly rectifying potassium channel 2 R92Q (State 1)
Translational activators Aep1, Aep2 and Atp25 in complex with mRNA and the yeast mitochondrial ribosome (Small ribosomal subunit)
Cryo-EM structure of Candida albicans pH regulated antigen 1 (Pra1) protein in the absence of Zn2+
Cryo-EM structure of Candida albicans pH regulated antigen 1 (Pra1) protein in complex with Zn2+
5-methyl-cytidine twist corrected RNA origami 6-helix bundle type-2 dimer
1-methyl-pseudouridine twist corrected RNA origami 6-helix bundle type-1 dimer
Cryo-EM structure of GPR155 contracted dimer in complex with cholesterol
Cryo-EM structure of the human UBR4/KCMF1/CALM1 complex (composite map)
Cryo-EM structure of mouse RNF213:UBE2L3 transthiolation intermediate, chemically stabilized, and ATPgS
Structure of SARS-CoV-2 spike-CD147 complex at 3.75 Angstroms resolution
Cryo-EM structure of Mycobacterium tuberculosis MmpL5 in complex with AcpM
Cryo-EM map of MSMEG_3496 in complex with AcpM, size exclusion chromatography peak1
Cryo-EM map of MSMEG_3496 in complex with AcpM, size exclusion chromatography peak2
Cryo-EM structure of the wild-type flagellar filament from Roseburia hominis
Structure of the Leishmania inner dyenin arm D and inner dynein arm G bases bound to the DMT
Structure of the Leishmania inner dyenin arm A base bound to the DMT
Structure of the Leishmania inner dyenin arm B base bound to the DMT
Structure of the Leishmania inner dyenin arm E base bound to the DMT
Structure of the Leishmania inner dyenin arm C base bound to the DMT
Structure of the Leishmania inner dyenin arm F base bound to the DMT
Structure of the Leishmania radial spoke 3 base portion 1 bound to the DMT
Structure of the Leishmania radial spoke 3 portion 2 bound to the DMT
microtubule doublet from Kif27-/- mouse tracheal epithelial cells
Microtubule doublet from wild-type mouse tracheal epithelial cells
Cryo-EM structure of human organic solute transporter Ost-alpha/beta bound with TLCA
Cryo-EM structure of human organic solute transporter Ost-alpha/beta bound with DHEAS
Cryo-EM structure of human organic solute transporter Ost-alpha/beta in apo state
2.7 A structure of the SiO2-sealed and revitrified (300 us) E.coli 50S ribosomal subunit
1.8 A structure of SiO2-sealed and revitrified (210 us) mouse heavy chain Apoferritin
2.4 A structure of the SiO2-sealed and revitrified (30 us) E.coli 50S ribosomal subunit
2.3 A structure of the SiO2-sealed and revitrified (150 us) E.coli 50S ribosomal subunit
10E8-GT10.2 immunogen in complex with Rhesus macaque Fab W3-05 and mouse Fab W6-03
10E8-GT10.2 immunogen in complex with Rhesus macaque Fab W3-02 and mouse Fab W6-03
10E8-GT10.2 immunogen in complex with mouse Fab W6-10 and rhesus macaque Fab W10-09
MPER-GT12 immunogen in complex with human Fab 10E8 and mouse Fab W6-10
Polyclonal immune complex of Fab from mice sera binding the top of N1 NA after immunization with inactivated split A/bald eagle/FL/W22-134-OP/2022 Influenza virus vaccine unadjuvanted
Polyclonal immune complex of Fab from mice sera binding the side of N1 NA after immunization with inactivated split A/bald eagle/FL/W22-134-OP/2022 Influenza virus vaccine adjuvanted with CpG
Polyclonal immune complex of Fab from mice sera binding the esterase of H5 HA after immunization with inactivated split A/bald eagle/FL/W22-134-OP/2022 Influenza virus vaccine adjuvanted with CpG
Polyclonal immune complex of Fab from mice sera binding the side of the head of H5 HA after immunization with inactivated split A/bald eagle/FL/W22-134-OP/2022 Influenza virus vaccine adjuvanted with CpG
Polyclonal immune complex of Fab from mice sera binding the head of H5 HA after immunization with inactivated split A/bald eagle/FL/W22-134-OP/2022 Influenza virus vaccine adjuvanted with CpG
HIV-1 integrase filament at the luminal side of capsid lattice by subtomogram averaging.
Cryo-tomogram of Agrobacterium phage 7-7-1 and Agrobacterium sp. H13-3
CryoEM reconstruction of integrase filament at the lumen of native HIV-1 cores (box size 47.3 nm)
Agrobacterium phage 7-7-1 neck 2 + tail terminator + tail sheath + tail tube
CryoEM reconstruction of integrase filament at the lumen of native HIV-1 cores (box size 34.2 nm)
ATP-bound human mitochondrial Hsp60-Hsp10 double-ring complex (D7)
ATP-bound human mitochondrial Hsp60-Hsp10 half-football complex (C7)
Structure of MPT domain of S. cerevisiae type-I FAS, thio-esterified to palmitate
Structure of ACP domain conjugated with stearic acid and interacting with MPT domain from M. tuberculosis type-I FAS
GH161 phosphorylase in complex with beta-1,3 glucooligosaccharide
Cryo-EM structure of human importin beta:importin alpha (IBB) complex
CryoEM structure of aldehyde dehydrogenase from Burkholderia cenocepacia at 2.33A resolution
Cryo-EM structure of human importin beta:Ran-GTP:RanBP1 trimeric complex
Negative Stain EM map of KSHV glycoprotein gHgL in complex with MLKH5 , MLKH10 and MLKH12 FABs.
Negative Stain EM map of KSHV glycoprotein gHgL in complex with MLKH1 FAB
Cryo-EM structure of KSHV glycoprotein gHgL in complex with MLKH3 and MLKH10 FABs
Negative Stain EM map of KSHV glycoprotein gHgL in complex with MLKH5 FAB
Negative Stain EM map of KSHV glycoprotein gHgL in complex with MLKH5, MLKH10 and MLKH6 FABs
Negative Stain EM map of KSHV glycoprotein gHgL in complex with MLKH5, MLKH10 and MLKH3 FABs.
Cryo-EM structure of light harvesting complex 2 from Ery. sanguineus
Cryo-EM structure of conivaptan-bound human vasopressin V2 receptor complex with Fab
Cryo-EM structure of tolvaptan-bound human vasopressin V2 receptor complex with Fab
Cryo-EM structure of SARS-CoV-2 spike protein in complex with three-nAb 8H12, 3E2 and 1C4
Target analog-bound type III-B Cmr complex of Archaeoglobus fulgidus
Cryo-EM structure of SARS-CoV-2 BA.5 spike protein in complex with nAb 1C4 (local refinement)
Cryo-EM structure of SARS-CoV-2 BA.1 spike protein in complex with three-nAb 8H12, 3E2 and 1C4
Cryo-EM structure of SARS-CoV-2 BA.2 spike protein in complex with triple-nAb 8H12, 3E2 and 1C4 (local refinement)
The NuA3 histone acetyltransferase complex bound to acetyl-CoA and H3 tail
Cryo-EM structure of the Arabidopsis thaliana CAT4 transporter in the outward-open apo state
Cryo-EM structure of LptDEM complex containing Shigella flexneri LptE and endogenous E. coli LptD and LptM
Hexahistidine-tagged tobacco mosaic virus coat protein 3-layer disk
Hexahistidine-tagged tobacco mosaic virus coat protein 4-layer disk
Herpes simplex virus 2 delta28-73 glycoprotein C (focus classification) ectodomain in complex with C3b
Cryo-EM structure of Shigella flexneri LptDE bound by phage RBP reveals N-terminal strand insertion into lateral gate
Ancestral Group III Chaperonin (ACIII) Double-Ring in Open Conformation including the Equatorial and Intermediate Domains (residues 12-200 and 356-507)
