Fourier Shell Correlation (FSC) server
Welcome to the PDBe Fourier Shell Correlation (FSC) server!
This web service will calculate and plot the FSC curve from two 3D reconstructions that you upload.
You will be able to download the FSC curve as an XML file that can then be uploaded
as a part of a deposition to EMDB. This server uses the EMAN2
package
for the FSC calculation. The implementation of the 3 sigma and half bit threshold
curves is based on Imagic code kindly provided by Michael Schatz (ImageScience
Software GmbH).
Supported input file formats
include CCP4/MRC, Imagic, Spider,
EMAN2 HDF5 and others. We have one test data sets that you can use to try out the service
here. We appreciate your continuing
support and
feedback!
The uploaded maps will be deleted after the calculation is completed.
Quick links
Recent Entries
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Porcine ATP synthase with inhibitory protein IF1, Peripheral Stalk domain, DP-state
Porcine ATP synthase with inhibitory protein IF1, F1 domain, DP-state
Porcine ATP synthase with inhibitory protein IF1, Fo domain, DP-state
Porcine ATP synthase without inhibitory protein IF1, F1 domain, DP-state
Porcine ATP synthase without inhibitory protein IF1, Peripheral Stalk domain, DP-state
Porcine ATP synthase without inhibitory protein IF1, Fo domain, DP-state
Human Serum Albumin in complex with four nanobodies: N33, nb139 & nb104
Cryo-EM Structure of Ryanodine Receptor 1: Drug Bound Open Conformation C-terminal Domain Locally Refined Map
Cryo-EM Structure of Ryanodine Receptor 1: Drug Bound Open Conformation Consensus Map
Cryo-EM Structure of Ryanodine Receptor 1: Drug Bound Open Conformation Transmembrane Domain (C4) Locally Refined Map
Cryo-EM Structure of Ryanodine Receptor 1: Drug Bound Open Conformation BSol Locally Refined Map
Cryo-EM Structure of Ryanodine Receptor 1: Drug Bound Open Conformation N-terminal Domain Locally Refined Map
Nucleosome with an SSB at SHL -2.8 in complex with the WGR domain of human PARP2, Class 1
Sub-tomogram averaged structure of the Tad pilus secretin in Caulobacter crescentus
Sub-tomogram averaged structure of the non-piliated Tad machine in Caulobacter crescentus
Sub-tomogram averaged structure of the piliated Tad machine in Caulobacter crescentus
Nucleosome with an SSB at SHL -2.8 in complex with the WGR domain of human PARP2, Class 2
Nucleosome with an SSB at SHL -2.8 in complex with human PARP2 and HPF1, Class 1
Nucleosome with an SSB at SHL -2.8 in complex with human PARP2 and HPF1, Class 2
Cryo-EM structure of human integrin alpha5beta1 in complex with fibronectin (FN 7-10)
NTSR1-G11-NTS(8-13) GTP-Bound Complex in the Canonical, AHD Closed State 3DVA Separated 1 (C-Closed-GTP)
NTSR1-Gi-NTS(8-13) GTP-Bound Complex in the Canonical, AHD Closed State, 3DVA Sorted (C-Closed*-GTP)
NTSR1-G11-NTS(8-13) Complex in the Canonical, AHD Partially Closed State (C-P-Closed-Apo)
NTSR1-G11-NTS(8-13) Complex in the Canonical, AHD Open State (C-Open-Apo)
NTSR1-Gi-NTS(8-13) Complex in the Canonical, AHD Closed State (C-Closed-Apo)
NTSR1-Gi-NTS(8-13) GTP-Bound Complex in the Non-Canonical, AHD Closed State (NC-Closed-GTP)
NTSR1-Gi-NTS(8-13), GTP-bound Complex in the Canonical, AHD Open State (C-Open-GTP)
NTSR1-Gi-NTS(8-13) Complex in the Canonical, AHD Open State (C-Open-Apo)
NTSR1-G11-NTS(8-13) GTP-Bound Complex in the Canonical, AHD Closed State 3DVA Separated 2 (C-Closed*-GTP)
NTSR1-Gi-NTS(8-13) GTP-Bound Complex in the Non-Canonical, AHD Open State (NC-Open-GTP)
NTSR1-G11-NTS(8-13) Complex in the Non-Canonical, AHD Open State (NC-Open-Apo)
NTSR1-Gi-NTS(8-13) Complex in the Non-Canonical, AHD Open State (NC-Open-Apo)
Cryo-EM structure of raiA motif RNA from Thermosediminibacter oceani
NTSR1-Gi-NTS(8-13), GTP-bound Complex in the Canonical, AHD Open State (C-Open-GTP), MSP1D1
NTSR1-Gi-NTS(8-13) GTP-Bound Complex in the Canonical, AHD Closed State (C-Closed-GTP), MSP1D1 Nanodisc
NTSR1-Gi-NTS(8-13) GTP-Bound Complex in the Canonical, AHD Closed State (C-Closed-GTP)
Homotrimer of the extracellular PPIase Mpn444 from Mycoplasma pneumoniae
Cryo-EM structure of the cage-top domain of the erlin1/2 complex purified using GDN and CHS
Focused refinement of beta-barrel assembly machine from Escherichia coli in an late state of LptD assembly
Consensus refinement of beta-barrel assembly machine from Escherichia coli in an late state of LptD assembly
Reconstituted TRRAP lobe of the human TIP60 complex, distal region (focused refinement)
Nucleosome with an SSB at SHL -2.8 in complex with the WGR domain of human PARP2, Class 1, Consensus map
CryoEM structure of computationally designed bundlemer peptide nanotube (14 protofilament)
Nucleosome with an SSB at SHL -2.8 in complex with the WGR domain of human PARP2, Class 2, Focused map
CryoEM structure of computationally designed bundlemer peptide nanotube (15 protofilament)
Nucleosome with an SSB at SHL -2.8 in complex with the WGR domain of human PARP2, Class 1, Focused
Nucleosome with an SSB at SHL -2.8 in complex with the WGR domain of human PARP2, Class 2, Consensus map
Cryo-EM structure of the reconstituted TRRAP lobe of the human TIP60 complex (consensus map filtered by local resolution)
Reconstituted TRRAP lobe of the human TIP60 complex, top region (focused refinement)
Reconstituted RuvBL lobe of the human TIP60 complex, top RUVBL region (focused refinement)
Reconstituted RuvBL lobe of the human TIP60 complex, bottom RUVBL region (focused refinement)
Cryo-EM structure of the reconstituted RuvBL lobe of the human TIP60 complex (consensus map filtered by local resolution)
Reconstituted RuvBL lobe of the human TIP60 complex, HSA module (focused refinement)
Reconstituted TRRAP lobe of the human TIP60 complex, core region (focused refinement)
Reconstituted TRRAP lobe of the human TIP60 complex, bottom region (focused refinement)
RuvBL lobe of the native human TIP60 complex, ATPase module (focused refinement)
SK5A-Matured glycine/glutamate in complex with GluN1-GluN2B, full refinement
SK5A-Matured apo state in complex with GluN1-GluN2B, full refinement
Cryo-EM structure of the Vo domain of V/A-ATPase in liposomes under no pmf condition,state2
Cryo-EM structure of the Vo domain of V/A-ATPase in liposomes under no pmf condition,state3
Egalitarian-BicD in complex with hSL1 and hSL2 of the hairy localization element
Egalitarian-BicD in complex with ILS of I-factor mRNA (Structure B)
Egalitarian-BicD in complex with hSL1 of the hairy localization element (Structure C)
Egalitarian-BicD in complex with bcdSLV of bicoid mRNA (Structure C)
Egalitarian-BicD in complex with ILS of I-factor mRNA (Structure A)
Desmotubule-coating assembly in protonemata of GHL17 Physcomitrium patens,centered on assembly surface
Egalitarian-BicD in complex with bcdSLV of bicoid mRNA (Structure B)
Egalitarian-BicD in complex with hSL1 of the hairy localization element (Structure B)
Egalitarian-BicD in complex with hSL1 of the hairy localization element (Structure A)
Egalitarian-BicD in complex with bcdSLV of bicoid mRNA (Structure A)
Cryo-ET of a plasmodesma in wild type Physcomitrium patens protonema tissue treated with abscisic acid
Cryo-ET of a plasmodesma in wild type Physcomitrium patens protonema tissue treated with abscisic acid
Cryo-ET of a plasmodesma in GHL17 Physcomitrium patens protonema tissue
Cryo-ET of a plasmodesma in wild type Physcomitrium patens protonema tissue
Cryo-EM structure of alphaM/beta2:C3d-anti-CR3-Nb headpiece complex (HPO1 3D class reconstruction)
Cryo-EM structure of alphaM/beta2:C3d-anti-CR3-Nb headpiece complex (HPO2 3D class reconstruction)
Cryo-EM structure of alphaM I-domain:C3d-anti-CR3-Nb complex focused refinement from the alphaM/beta2:C3d-anti-CR3-Nb headpiece complex
Cryo-ET of a plasmodesma in wild type Physcomitrium patens gametophore leaflet tissue
Cryo-ET of a plasmodesma in GHL17 Physcomitrium patens protonema tissue
Cryo-ET of a plasmodesma in wild type Physcomitrium patens gametophore leaflet tissue
Cryo-ET of a plasmodesma in wild type Physcomitrium patens protonema tissue
Cryo-EM structure of alphaM/beta2:MEM148-Fab headpiece complex (without alphaM I-domain)
Engineering the ADDomer Nanoparticle Vaccine Scaffold for Improved Assembly and Enhanced Stability.
Human TRPM8 fully-swapped, ligand-free structure in the absence of calcium at 4 degrees Celsius resolved in cell vesicles
Avian TRPM8 (Parus major) fully-swapped, closed, ligand-free in the presence of calcium, structure resolved in cell vesicles
Avian TRPM8 (Parus major) fully swapped closed, calcium free, in the presence of menthol, resolved in cell vesicles
Avian TRPM8 (Parus major) semi-swapped, closed, calcium free, menthol bound structure resolved in cell vesicles
Human TRPM8 menthol bound structure at 4 degrees Celsius resolved in cell vesicles using cryo-EM
Avian TRPM8 (Parus major) semi-swapped, ligand-free structure resolved in cell vesicles using cryo-EM
Avian TRPM8 (Parus major) semi-swapped, ligand-free structure at high pH and 4 degrees Celsius resolved in GDN using cryo-EM
Avian TRPM8 (Parus major) semi-swapped, closed, ligand-free in the presence of calcium, structure resolved in cell vesicles
Human TRPM8 closed, ligand-free structure resolved in GDN using cryo-EM
Avian TRPM8 (Parus major) closed, ligand-free structure resolved in cell vesicles using cryo-EM
Human TRPM8 V915Y fully-swapped, closed, ligand-free structure resolved in GDN
Human TRPM8 fully-swapped, desensitized, ligand-free structure at 4 degrees Celsius resolved in cell vesicles
Parus major TRPM8 with a chimeric human outer pore loops, semi-swapped, ligand-free, cold, structure resolved in GDN
Cryo-EM structure of Human UBA1-UBE2O-Ub -Recruitment state 1 (A)
Cryo-EM structure of Human UBA1-UBE2O-Ub -Transthiolation state 4
Cryo-EM structure of Human UBA1-UBE2O-Ub -Recruitment state 1 (B)
Cryo-EM structure of Human UBA1-UBE2O-Ub -Transthiolation state 3
Cryo-EM structure of Human UBA1-UBE2O-Ub -Recruitment state 1 (consensus map)
Cryo-EM structure of Human UBA1-UBE2O-Ub -Transthiolation state 2
Cryo-EM structure of depolymerase S2-4 from Klebsiella phage K64-1
Cryo-EM structure of depolymerase S2-4 from Klebsiella phage K64-1
Cryo-EM structure of Human UBA1-UBE2O-Ub -Transthiolation state 1
Cryo-EM density map of the 96-nm repeat of the human respiratory doublet microtubule
Cryo-EM density map of the 48-nm repeat of the CCDC114-mutant PCD patient respiratory doublet microtubule with c.705_706insGCAG mutation
Cryo-EM density map of the 48-nm repeat of the human respiratory doublet microtubule
Cyro-EM density map of the 96-nm repeat of the CCDC114-mutant PCD patient respiratory doublet microtubule with c.-41-2A>C mutation
Cryo-EM structures of the Azoarcus pre-tRNA(ILE) intron conformation after the cyclization
Cryo-EM structures of the Azoarcus pre-tRNA(ILE) conformation a undergoing the first-step self-splicing
Cryo-EM structures of the Azoarcus pre-tRNA(ILE) conformation b undergoing the first-step self-splicing
Cryo-EM structures of the Azoarcus pre-tRNA(ILE) intron conformation after the self-splicing
Cryo-EM density map of the 48-nm repeat of the CCDC114-mutant PCD patient respiratory doublet microtubule with c.-41-2A>C mutation
Cryo-EM structure of the Azoarcus sp. BH72 pre-tRNA(ILE) intron after second-step cyclization (circular form)
HRV14 3C in complex with single chain antibody YDF and cloverleafRNA-conformation1-composite-1
HRV14 3C in complex with single chain antibody YDF and cloverleafRNA-conformation1-composite-2
HRV14 3C in complex with single chain antibody YDF and cloverleafRNA-conformation 2
Substrate-engaged human 26S proteasome bound to midnolin with RPT1 at top of spiral staircase
HRV14 3C in complex with single chain antibody YDF and cloverleafRNA-conformation1
Substrate-engaged human 26S proteasome bound to midnolin with RPT2 at top of spiral staircase
Focused refinement of RPN1 and the C-terminal helix of midnolin in the substrate-engaged human 26S proteasome
Focused refinement of 19S in the substrate-engaged human 26S proteasome bound to midnolin with RPT6 at top of spiral staircase
Substrate-engaged human 26S proteasome bound to midnolin with RPT5 at top of spiral staircase
HRV14 3C in complex with single chain antibody YDF and cloverleafRNA-dimer conformation
reduced NorQ-NorD VWA domain complex from Paracoccus denitrificans
NorQ in complex with NorD VWA domain steep staircase subset 2 from Paracoccus denitrificans
NorQ in complex with NorD VWA domain from Paracoccus denitrificans
NorQ in complex with NorD VWA domain steep staircase subset 1 from Paracoccus denitrificans
Tetrameric cystathionine beta-synthase of Mycobacterium tuberculosis bound to AOAA
Tetrameric cystathionine beta-synthase of Mycobacterium tuberculosis bound to O-Benzylhydroxylamine
Structural mechanism of substrate binding of the human Proline Transporter
Structure of the apo-state human proline transporter purified in DDM buffer
Structure of the intial complex in filament assembly at 3.23 angstroms resolution, conformation 2.
Cryo-EM structure of the erlin1/2 complex purified using DDM and GDN
Cryo-EM structure of the erlin1/2 complex purified using GDN and CHS
Cryo-EM overall structure of the erlin1/2 complex purified using GDN and CHS
Structure of the cholesterol-bound human proline transporter purified in DDM buffer
Structure of the L-proline-free human proline transporter purified in DDM buffer
Structure of the apo-state human proline transporter purified in DDM/CHS buffer
Structure of the L-proline-free human proline transporter purified in DDM/CHS buffer
Structure of the cholesterol-bound human proline transporter purified in DDM/CHS buffer
Cryo-EM overall structure of the erlin1/2 complex purified using DDM and GDN
Cryo-EM structure of the cage-top domain of the erlin1/2 complex purified using DDM and GDN
Human Serum Albumin in complex with four nanobodies: nb118, nb68, N30 & N23
Human Serum Albumin in complex with four nanobodies: nb33, nb77, nb125 & N42
Human Serum Albumin in complex with four nanobodies: N33, nb139, nb39 & nb104
Rubisco subtomogram average from chloroplasts of two weeks old Physcomitrium patens protonemata cells.
Cryo-EM structure of apo form of Arthrobacter psychrolactophilus L-arabinose isomerase
Structure of the YbjP lipoprotein bound to the MacAB-TolC tripartite efflux pump