Fourier Shell Correlation (FSC) server
Welcome to the PDBe Fourier Shell Correlation (FSC) server!
This web service will calculate and plot the FSC curve from two 3D reconstructions that you upload.
You will be able to download the FSC curve as an XML file that can then be uploaded
as a part of a deposition to EMDB. This server uses the EMAN2
package
for the FSC calculation. The implementation of the 3 sigma and half bit threshold
curves is based on Imagic code kindly provided by Michael Schatz (ImageScience
Software GmbH).
Supported input file formats
include CCP4/MRC, Imagic, Spider,
EMAN2 HDF5 and others. We have one test data sets that you can use to try out the service
here. We appreciate your continuing
support and
feedback!
The uploaded maps will be deleted after the calculation is completed.
Quick links
Recent Entries
(Show all)
Dual receptor-binding, infectivity, and transmissibility of an emerging H2N2 avian influenza virus
Structure of HBV surface antigen determined in recombinant spherical subviral particle
Cryo-EM structure of SARS-CoV-2 spike ectodomain (HexaPro, Omicron BA.5 variant) in complex with CeSPIACE, class 1
Cryo-EM structure of human proteasome assembly intermediate half-proteasome
SpCas9-MMLV RT-pegRNA-target DNA complex (elongation 16-nt) focused map
Cryo-EM structure of SARS-CoV-2 spike ectodomain (HexaPro, Omicron BA.5 variant) in complex with CeSPIACE, class 2
Dual receptor-binding, infectivity, and transmissibility of an emerging H2N2 avian influenza virus
SpCas9-MMLV RT-pegRNA-target DNA complex (elongation 28-nt) overall map
Dual receptor-binding, infectivity, and transmissibility of an emerging H2N2 avian influenza virus
Cryo-EM structure of the catalytic domain and an agarose-binding domain (CBM6) of alpha-agarase from Catenovulum maritimum STB14
CryoEM structure of human XPR1 in complex with phosphate in state A
Structure of Epstein-Barr virus major glycoprotein gp350 in complex with the receptor CR2
CryoEM structure of human XPR1 in complex with phosphate in state B
CryoEM structure of human XPR1 in complex with phosphate in state C
Xenopus borealis undecorated microtubule - 15 protofilament, 3-start helix
Candida albicans gamma-tubulin small complex within ring-like higher oligomer in complex with Spc72 CM1
CryoEM structure of the antiparallel double-stranded influenza A RNP-like particle with a 18-mer RNA.
HIV-1 CA hexamer from nuclear capsids (FIB-lamella data of HIV infected macrophages)
HIV-1 CA hexamer from capsids inside NPCs (FIB-lamella data of HIV infected macrophages)
Human RNA Polymerase III Class III Open Pre-initiation Complex 1 (OC1)
CryoEM structure of influenza A RNP-like particle double-stranded assembled with a 12-mer RNA.
In situ human cytoplasmic ring subunit of nuclear pore complex (FIB-lamella data of mock infected macrophages)
CryoEM structure of catalytic domain of human HMG-CoA reductase with its inhibitor atorvastatin
RNA Polymerase III Class III Open Mini Pre-initiation Complex 1 (OC1-mini)
Cryo-EM structure of Saccharolobus solfataricus 30S initiation complex bound to Ss-MAP leaderless mRNA
DNA helicase RECQL5 in complex with homo Di-Gluebody G5-006 - RECQL5 local refinement
HIV-1 CA hexamer from cytoplasmic capsids (FIB-lamella data of HIV infected macrophages)
Focused reconstruction on strand 1 of the influenza A RNP-like particle double-stranded assembled with a 18-mer RNA.
HIV-1 CA hexamer from capsids inside virions (FIB-lamella data of HIV infected macrophages)
RECQL5:sfGFP hetero dimer assembled by Di-Gluebody - sfGFP local refinement
PF30S-PF30S dimer mediated by aRDF from P. furiosus (Structure II)
In situ human asymmetric subunit of nuclear pore complex (FIB-lamella data of mock infected macrophages)
Cryo-EM structure of Saccharolobus solfataricus 30S initiation complex bound to Ss-aIF2beta leaderless mRNA
Xenopus tropicalis undecorated microtubule - 14 protofilament, 3-start helix
Cryo-EM structure of Saccharolobus solfataricus 30S initiation complex bound to Ss-aIF2beta leaderless mRNA with h44 in up position
RECQL5:sfGFP hetero dimer assembled by Di-Gluebody - RECQL5 local refinement
Full gamma-tubulin ring complex composed of the Candida albicans gamma-tubulin small complex in complex with Spc72 CM1
Xenopus laevis undecorated microtubule - 15 protofilament, 4-start helix
High-resolution cryo-EM structure of Saccharolobus solfataricus 30S ribosomal subunit bound to mRNA and initiator tRNA
CryoEM structure of influenza A RNP-like particle single-stranded assembled with a 12-mer RNA.
Cryo-EM structure of Saccharolobus solfataricus 30S initiation complex bound to SD mRNA
In situ human luminal ring subunit of nuclear pore complex (FIB-lamella data of HIV infected macrophages)
HIV-1 CA pentamer from capsids inside virions (FIB-lamella data of HIV infected macrophages)
PF30S-PF30S dimer mediated by aRDF from P. furiosus (Structure I)
Cryo-EM structure of Pseudomonas aeruginosa Recombinase A (RecA) in complex with LexAS125A mutant
SPNS2:sfGFP hetero dimer assembled by Di-Gluebody - sfGFP local refinement
HIV-1 CA pentamer from cytoplasmic capsids (FIB-lamella data of HIV infected macrophages)
Xenopus laevis undecorated microtubule - 14 protofilament, 3-start helix
Cryo-EM structure of Saccharolobus solfataricus 30S ribosomal subunit with helix 44 in position down bound to mRNA and initiator tRNA
Cryo-EM structure of Saccharolobus solfataricus 30S initiation complex bound to Ss-MAP leaderless mRNA with h44 in up position
In situ human cytoplasmic ring subunit of nuclear pore complex (FIB-lamella data of HIV infected macrophages)
Xenopus tropicalis undecorated microtubule - 15 protofilament, 3-start helix
Cryo-EM structure of Saccharolobus solfataricus 30S initiation complex bound to SD mRNA with h44 in up position
In situ human asymmetric subunit of nuclear pore complex (FIB-lamella data of HIV infected macrophages)
Cryo-EM structure of Pseudomonas aeruginosa recombinase A (RecA) in complex with ssDNA 72mer and ATPgS
Xenopus borealis undecorated microtubule - 14 protofilament, 3-start helix
Focused reconstruction on strand 2 of the influenza A RNP-like particle double-stranded assembled with a 18-mer RNA.
In situ human basket attachment to nuclear ring of nuclear pore complex (FIB-lamella data of mock infected macrophages)
SPNS2:sfGFP hetero dimer assembled by Di-Gluebody - SPNS2 local refinement
In situ human inner ring subunit of nuclear pore complex (FIB-lamella data of HIV infected macrophages)
In situ human symmetrized composite map of nuclear pore complex (FIB-lamella data of HIV infected macrophages)
In situ human nuclear ring subunit of nuclear pore complex (FIB-lamella data of HIV infected macrophages)
In situ human luminal ring subunit of nuclear pore complex (FIB-lamella data of mock infected macrophages)
CryoEM structure of influenza A RNP-like particle double-stranded assembled with a 14-mer RNA.
In situ human symmetrized composite map of nuclear pore complex (FIB-lamella data of mock infected macrophages)
In situ human inner ring subunit of nuclear pore complex (FIB-lamella data of mock infected macrophages)
RNA Polymerase III Class III Open Mini Pre-Initiation complex 2 (OC2-mini)
Xenopus laevis undecorated microtubule - 15 protofilament, 3-start helix
In situ human nuclear ring subunit of nuclear pore complex (FIB-lamella data of mock infected macrophages)
SPNS2 in complex with homo Di-Gluebody GbD12 - SPNS2 local refinement
In situ human basket attachment to nuclear ring of nuclear pore complex (FIB-lamella data of HIV infected macrophages)
The Outer Dynein Arm-Docking Complex (ODA-DC) from human fallopian tube
The Outer Dynein Arm-Docking Complex (ODA-DC) from bovine Fallopian tube
The nexin-dynein regulatory complex (N-DRC) from bovine Fallopian tube, distal part
Hydrogen Peroxide-bound Vanadium-dependent Bromoperoxidase from Corallina pilulifera
SARS-CoV-2 Wuhan Spike ectodomain in complex with NHP polyclonal antibody NVX-NHPSA3 (NVX-CoV2373/rS-Beta immunized)
Kappa opioid receptor:Galphai protein in complex with inverse agonist JDTic
Subtomogram average of capsid (CA) from immature Enveloped Delivery Vehicles (EDVs) without Cas9
SARS-CoV-2 Wuhan Spike ectodomain in complex with NHP polyclonal antibody NVX-NHPWu2 (NVX-CoV2373 vaccine)
SARS-CoV-2 Wuhan Spike ectodomain in complex with NHP polyclonal antibody NVX-NHPSA1 (NVX-CoV2373/rS-Beta immunized)
Azotobacter vinelandii filamentous 2:2:1 MoFeP:FeP:FeSII-Complex (C2 symmetry)
DNA Polymerase Beta bound to a nucleosome containing a 1-nt gap at SHL-4.5 (State 1, composite)
DNA Polymerase Beta bound to a nucleosome containing a 1-nt gap at SHL-4.5 (State 1, Pol Beta/DNA local refine)
DNA Polymerase Beta bound to a nucleosome containing a 1-nt gap at SHL-3.5
CryoEM Structure of a FtsH Helical Assembly in the Presence of ATP
Azotobacter vinelandii filamentous 2:2:1 MoFeP:FeP:FeSII-Complex (termini; C1 symmetry)
Kappa opioid receptor:Galphai protein in complex with inverse agonist norBNI
DNA Polymerase Beta bound to a nucleosome containing a 1-nt gap at SHL-4.5 (State 3, Pol B/DNA local refine)
Formation of I2+III2 supercomplex rescues respiratory chain defects
SARS-CoV-2 Wuhan Spike ectodomain in complex with NHP polyclonal antibody NVX-NHPSA4 (NVX-CoV2373/rS-Beta immunized)
DNA Polymerase Beta bound to a nucleosome containing a 1-nt gap at SHL-4.5 (State 1, consensus)
Bromide-bound Vanadium-dependent Bromoperoxidase from Corallina pilulifera
The nexin-dynein regulatory complex (N-DRC) from human fallopian tube, base part
Fascin crosslinked F-actin hexagonal bundle element (740 Angstrom box)
DNA Polymerase Beta bound to a nucleosome containing a 1-nt gap at SHL-4.5 (State 3, consensus)
Kappa opioid receptor in complex with heterotrimerig Gi protein, bound to inverse agonist GB18
SARS-CoV-2 Wuhan Spike ectodomain in complex with NHP polyclonal antibody NVX-NHPWu3 (NVX-CoV2373 vaccine)
cryo-EM structure of fascin crosslinked F-actin (containing both actin binding sites)
SARS-CoV-2 Wuhan Spike ectodomain in complex with NHP polyclonal antibody NVX-NHPSA7 (NVX-CoV2373/rS-Beta immunized)
TolQ-TolR inner membrane protein complex from Acinetobacter baumannii
Non-canonical E.coli 100S ribosome imaged in situ with rapid processing pipeline
DNA polymerase Beta bound to a nucleosome containing a 1-nt gap at SHL-5.5
DNA Polymerase Beta bound to a nucleosome containing a 1-nt gap at SHL-5.5 (Pol B/DNA local refine)
Cryo-EM structure of fascin bound to F-actin (actin binding site 1)
Structure of the human mitochondrial Hsp70 (mortalin; R126W mutant) bound to nucleotide exchange factor GrpEL1 (WT)
Xenorhabdus nematophilus XptA2 State 2, 1181insYWK1183, D1182T mutant
SARS-CoV-2 Wuhan Spike ectodomain in complex with NHP polyclonal antibody NVX-NHPWu7 (NVX-CoV2373 vaccine)
SARS-CoV-2 Wuhan Spike ectodomain in complex with NHP polyclonal antibody NVX-NHPWu6 (NVX-CoV2373 vaccine)
SARS-CoV-2 Wuhan Spike ectodomain in complex with NHP polyclonal antibody NVX-NHPWu5 (NVX-CoV2373 vaccine)
Dynein motor inner dynein arm subform e (IDAe) from bovine Fallopian tube
DNA Polymerase Beta bound to a nucleosome containing a 1-nt gap at SHL-4.5 (State 3, composite)
Structure of the human mitochondrial Hsp70 (mortalin; R126W mutant) missing SBD-a lid bound to nucleotide exchange factor GrpEL1 (Y173A mutant)
Human proton sensing receptor GPR4 in complex with miniGs - focused map of 7TM
Dynein motor inner dynein arm subform f (IDAf) from bovine Fallopian tube
DNA Polymerase Beta bound to a nucleosome containing a 1-nt gap at SHL-5.5 (consensus)
The nexin-dynein regulatory complex (N-DRC) from bovine Fallopian tube, base part
Kappa opioid receptor:Galphai protein in complex with inverse agonist JDTic , no scFv16
DNA Polymerase Beta bound to a nucleosome containing a 1-nt gap at SHL-4.5 (State 2, Pol Beta/DNA local refine)
PRC2_AJ1-450 bound to H3K36me3-modified nucleosome with histone H3 tail disengaged
Cryo-EM structure of fascin bound to F-actin (actin binding site 2)
SARS-CoV-2 Wuhan Spike ectodomain in complex with NHP polyclonal antibody NVX-NHPWu1 (NVX-CoV2373 vaccine)
SARS-CoV-2 Wuhan Spike ectodomain in complex with NHP polyclonal antibody NVX-NHPSA6 (NVX-CoV2373/rS-Beta immunized)
SARS-CoV-2 Wuhan Spike ectodomain in complex with NHP polyclonal antibody NVX-NHPSA2 (NVX-CoV2373/rS-Beta immunized)
Cryo-EM structure of human core Rab3GAP1/2 complex, local refinement
SARS-CoV-2 Wuhan Spike ectodomain in complex with NHP polyclonal antibody NVX-NHPSA8 (NVX-CoV2373/rS-Beta immunized)
Structure of the human mitochondrial Hsp70 (mortalin; R126W mutant) bound to nucleotide exchange factor GrpEL1 (Y173A mutant)
SARS-CoV-2 Wuhan Spike ectodomain in complex with NHP polyclonal antibody NVX-NHPWu4 (NVX-CoV2373 vaccine)
CryoEM Structure of a FtsH Helical Assembly in the Presence of ATP
SARS-CoV-2 Wuhan Spike ectodomain in complex with NHP polyclonal antibody NVX-NHPSA5 (NVX-CoV2373/rS-Beta immunized)
DNA Polymerase Beta bound to a nucleosome containing a 1-nt gap at SHL-4.5 (State 2, consensus)
Vanadate-bound Vanadium-dependent Bromoperoxidase from Corallina pilulifera
Subtomogram average of capsid (CA) from immature Enveloped Delivery Vehicles (EDVs) containing Cas9
DNA Polymerase Beta bound to a nucleosome containing a 1-nt gap at SHL-4.5 (State 2, composite)
Human proton sensing receptor GPR68 in complex with miniGsq - focused map of 7TM
Fascin crosslinked F-actin hexagonal bundle element (460 Angstrom box)
Azotobacter vinelandii 1:1:1 MoFeP:FeP:FeSII-Complex (C1 symmetry)
SpCas9-MMLV RT-pegRNA-target DNA complex (termination) focused map
SpCas9-MMLV RT-pegRNA-target DNA complex (termination) overall map
Dual receptor-binding, infectivity, and transmissibility of an emerging H2N2 avian influenza virus
Cryo-EM structure of human proteasome assembly intermediate preholo-1
Dual receptor-binding, infectivity, and transmissibility of an emerging H2N2 avian influenza virus
SpCas9-MMLV RT-pegRNA-target DNA complex (elongation 16-nt) overall map
SpCas9-MMLV RT-pegRNA-target DNA complex (elongation 28-nt) focused map
Cryo-EM structure of SARS-CoV-2 spike ectodomain (HexaPro, Omicron BA.2 variant) in complex with CeSPIACE
Cryo-EM structure of human proteasome assembly intermediate preholo-2
Structure of the sea urchin spSLC9C1 in state-3 w/o cAMP protomer
Dual receptor-binding, infectivity, and transmissibility of an emerging H2N2 avian influenza virus
Cryo-EM structure of human DNMT1 (aa:351-1616) in complex with ubiquitinated PAF15 and hemimethylated DNA analog
Dual receptor-binding, infectivity, and transmissibility of an emerging H2N2 avian influenza virus