Fourier Shell Correlation (FSC) server
Welcome to the PDBe Fourier Shell Correlation (FSC) server!
This web service will calculate and plot the FSC curve from two 3D reconstructions that you upload.
You will be able to download the FSC curve as an XML file that can then be uploaded
as a part of a deposition to EMDB. This server uses the EMAN2
package
for the FSC calculation. The implementation of the 3 sigma and half bit threshold
curves is based on Imagic code kindly provided by Michael Schatz (ImageScience
Software GmbH).
Supported input file formats
include CCP4/MRC, Imagic, Spider,
EMAN2 HDF5 and others. We have one test data sets that you can use to try out the service
here. We appreciate your continuing
support and
feedback!
The uploaded maps will be deleted after the calculation is completed.
Quick links
Recent Entries
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LSU structure derived from the LSU sample of the mitoribosome from T. gondii.
SSU(head) structure derived from the SSU sample of the mitoribosome from T. gondii.
30S mRNA delivery complex TEC resolved (30S only) focused body map
30S-TEC (TEC in expressome position) Inactive state 1, focused platform map
Cryo-EM structure of SKP1-FBXO22 in complex with a BACH1 BTB dimer at 3.2A resolution
Cryo-EM map of conventional 50S ribosomal subunit used as control for shaped pulses
SSU(body) structure derived from the SSU sample of the mitoribosome from T. gondii.
30S-TEC (TEC in expressome position) Inactive state 1, focused body map
30S-TEC (TEC in expressome position) Inactive state 1, focused head map
30S mRNA delivery complex TEC resolved (30S only) focused platform map
LSU structure derived from the monosome sample of the mitoribosome from T. gondii.
RNA polymerase II bound to minimal Alu RNA right arm in the canonical conformation
Cryo-EM structure of the decameric TraT surface exclusion lipoprotein from Escherichia coli (F plasmid)
Cryo-EM structure of the decameric TraT surface exclusion lipoprotein from Klebsiella pneumoniae (pKpQIL plasmid)
30S mRNA delivery complex TEC resolved (30S only) focused head map
Cryo-EM structure of the inward-facing choline-bound FLVCR2 with heme
30S-TEC (TEC in expressome position) Inactive state 1, consensus map
30S-TEC (TEC in expressome position) Inactive state 2, focused platform map
30S-TEC (TEC in expressome position) Inactive state 2, consensus map
RNA polymerase II bound to minimal Alu RNA right arm in the alternative conformation
cryo-EM structure of carboxysomal mini-shell icosahedral assembly from co-expression of CsoS1C, CsoS4A, and CsoS2-C (T = 9)
30S-TEC (TEC in expressome position) Inactive state 1, focused TEC map
30S-TEC (TEC in expressome position) Inactive state 2, focused head map
Cryo-EM reconstruction of TC-NER transcription elongation complex with STK19 (Consensus refinement)
Structure of endogenous Actin filament from rat model of Alzheimer's disease
Cryo-EM reconstruction of TC-NER transcription elongation complex with STK19 (map VIII)
Hypomethylated yeast 80S bound with Taura syndrome virus (TSV) internal ribosome entry site (IRES), eEF2, GDP, and sordarin, Structure III
Hypomethylated yeast 80S bound with Taura syndrome virus (TSV) internal ribosome entry site (IRES), eEF2, GDP, and sordarin, Structure II
Cryo-EM structure of tau filaments made from jR2R3-P301L peptides induced with heparin
Kinetic intermediate states of HIV-1 RT DNA synthesis captured by cryo-EM
RCK1-RCK2 double mutant of human Slo1 in presence of EDTA - resting VSD
Ligand-binding and transmembrane domains of kainate receptor GluK2 in complex with positive allosteric modulator BPAM-344 and channel blocker Spermine
Cryo-EM structure of mammalian Thorase filament, global helical refinement
Kinetic intermediate states of HIV-1 RT DNA synthesis captured by cryo-EM
Structure of murine heavy chain apoferritin collected at 200 keV with the Gatan Alpine direct detector
Structure of endogenous DPYSL2 from rat model of Alzheimer's disease
Rabbit RyR1 disease mutant Y523S in complex with FKBP12.6, nanodisc and inhibitor dantrolene in the absence of calcium
Kinetic intermediate states of HIV-1 RT DNA synthesis captured by cryo-EM
CryoEM structure of human GABAA receptor pi (GABRP) in complex with GABA
Kinetic intermediate states of HIV-1 RT DNA synthesis captured by cryo-EM
Cryo-EM structure of the pentadecameric DES-1 collagen-like assembly
Hypomethylated yeast 80S bound with Taura syndrome virus (TSV) internal ribosome entry site (IRES), eEF2, GDP, and sordarin, Structure I
RyR1 disease mutant Y523S with FKBP12.6, nanodisc and inhibitor dantrolene in the absence of calcium with refined P1 domain
Rabbit RyR1 disease mutant Y523S in complex with FKBP12.6, nanodisc and inhibitor dantrolene in the presence of activating calcium
Kinetic intermediate states of HIV-1 RT DNA synthesis captured by cryo-EM
Hypomethylated yeast 80S bound with Taura syndrome virus (TSV) internal ribosome entry site (IRES), eEF2, GDP, and sordarin, Structure IV
Cryo-EM reconstruction of TC-NER transcription elongation complex with STK19 (map IV)
Ligand-binding and transmembrane domains of kainate receptor GluK2 in complex with positive allosteric modulator BPAM-344 and channel blocker Philanthotoxin-74
Hypomethylated yeast 80S bound with cycloheximide, P-site tRNA, A-site tRNA, messenger RNA and eIF5A, PRE
Kinetic intermediate states of HIV-1 RT DNA synthesis captured by cryo-EM
Hypomethylated yeast 80S bound with Taura syndrome virus (TSV) internal ribosome entry site (IRES), eEF2, GDP, sordarin, and hibernating factor Los2
cryoEM map of endogenous V1 region of V-ATPase from rat model of Alzheimer's disease
Structure of murine heavy chain apoferritin collected at 300 keV with the Gatan K3 BioQuantum detector
Cryo-EM structure of mammalian Thorase filament, 3-layer refinement
Kinetic intermediate states of HIV-1 RT DNA synthesis captured by cryo-EM
Cryo-EM reconstruction of TC-NER transcription elongation complex with STK19 (map II)
E. coli DnaB bound to three DnaG C-terminal domains, ssDNA, ADP and AlF4
Structure of murine heavy chain apoferritin collected at 200 keV with the Gatan K3 detector
Cryo-EM structure of the DES-1 collagen-like peptide octadecameric assembly with C6 symmetry
Kinetic intermediate states of HIV-1 RT DNA synthesis captured by cryo-EM
Cryo-EM reconstruction of TC-NER transcription elongation complex with STK19 (map III)
Kinetic intermediate states of HIV-1 RT DNA synthesis captured by cryo-EM
Octahedral small virus-like particles of dengue virus type 2 (local reconstruction)
Low-resolution cryo-EM structure of the DES-1 collagen-like peptide doublet assembly
Ligand-binding and transmembrane domains of kainate receptor GluK2 in complex with positive allosteric modulator BPAM-344 and channel blocker Nephilatoxin-8
Structure of endogenous Actin filament from rat model of Alzheimer's disease
Hypomethylated yeast 80S bound with cycloheximide, P-site tRNA, and A-site tRNA, messenger RNA, POST
Cryo-EM reconstruction of TC-NER transcription elongation complex with STK19 (map VI)
Rabbit RyR1 disease mutant Y523S in complex with FKBP12.6, nanodisc and inhibitor dantrolene in the presence of activating calcium
Ligand-binding and transmembrane domains of kainate receptor GluK2 in complex with positive allosteric modulator BPAM-344 and channel blocker Kukoamine-A
Structure of murine heavy chain apoferritin collected at 120 keV with the Gatan Alpine direct detector
Cryo-EM reconstruction of TC-NER transcription elongation complex with STK19 (map V)
Kinetic intermediate states of HIV-1 RT DNA synthesis captured by cryo-EM
Kinetic intermediate states of HIV-1 RT DNA synthesis captured by cryo-EM
Hypomethylated yeast 80S bound with cycloheximide, P-site tRNA, and A-site tRNA, messenger RNA, PRE
Cryo-EM structure of the SPA-human octadecameric collagen-like assembly
Hypomethylated yeast 80S bound with Taura syndrome virus (TSV) internal ribosome entry site (IRES), eEF2, GDP, and sordarin, Structure V
Octahedral small virus-like particles of dengue virus type 2 (octahedral reconstruction)
Latrophilin-3 (ADGRL3) HormR and GAIN domains in the context of the holoreceptor
Hypomethylated yeast 80S bound with cycloheximide, unmodified U2921, mid rotated
Cryo-EM reconstruction of TC-NER transcription elongation complex with STK19 (map VII)
Structure of endogenous asymmetric DPYSL2 from rat model of Alzheimer's disease
Hemagglutinin H1 New Caledonia 1999 in complex with monoclonal antibody Fab 43_S0008
cryo-EM structure of carboxysomal midi-shell:icosahedral assembly from CsoS4A/4B/1A/1B/1C/1D and CsoS2 C-terminal co-expression (T = 19)
Cryo-EM structure of Cas12j19 (E100K), crRNA and target DNA complex
cryo-EM structure of carboxysomal midi-shell: icosahedral assembly from CsoS4A/4B/1A/1B/1C/1D and CsoS2 C-terminal co-expression (T = 13)
Cryo-EM structure of NRG1A(LRR) in complex with EDS1-SAG101-(ADPr-ATP)
cryo-EM structure of carboxysomal midi-shell: icosahedral assembly from CsoS4A/4B/1A/1B/1C/1D and CsoS2 C-terminal co-expression (T = 16)
Apo form of Tripartite ATP-independent Periplasmic (TRAP) transporter from Fusobacterium nucleatum.
cryo-EM structure of carboxysomal midi-shell: assembly from CsoS4A/4B/1A/1B/1C/1D and CsoS2 C-terminal co-expression (T=9 Q=12)
cryo-EM structure of carboxysomal midi-shell: icosahedral assembly from CsoS4A/4B/1A/1B/1C/1D and CsoS2 C-terminal co-expression (T = 9)
Cryo-EM structure of Mycobacterium tuberculosis transcription activation complex with six PhoP molecules
Sialic acid bound form of Tripartite ATP-independent Periplasmic (TRAP) transporter from Fusobacterium nucleatum.
State 7 (S7) of yeast 80S ribosome bound to 2 tRNAs and eEF2 during tranlocation
State 8a (S8a) of yeast 80S ribosome bound to 3 tRNAs and eEF1A and eEF3 during mRNA decoding
Cryo-EM structure of the E. coli BrxX methyltransferase complexed with Ocr
Subtomogram averaging of the C2S2M2L2-type PSII-LHCII supercomplex from Chlamydomonas reihardtii
State 8 (S8) of yeast 80S ribosome bound to 3 tRNAs and eEF1A during mRNA decoding
State 4 (S4) of yeast 80S ribosome bound to 2 tRNAs and eEF2 during translocation
State 2a (S2a) of yeast 80S ribosome bound to 2 tRNAs during peptidyl transfer
State 2c(S2c) of yeast 80S ribosome bound to compact eEF2 and 2 tRNAs during peptidyl transferation
Focused map for area 2 of type III CRISPR-associated deaminase in complex 2cA6 and 2ATP, partial activated
State 2f (S2f) of yeast 80S ribosome bound to 2 tRNAs during peptidyl transfer
State 1 (S1) of yeast 80S ribosome bound to 2 tRNAs and eEF1A during mRNA decoding
State 5 (S5) of yeast 80S ribosome bound to 2 tRNAs and eEF2 during translocation
Focused map for area 1 of type III CRISPR-associated deaminase in complex 2cA6 and 2ATP, partial activated
State 2d (S2d) of yeast 80S ribosome bound to compact eEF2 and 2 tRNAs during peptidyl transfer
State 2e (S2e) of yeast 80S ribosome bound to 2 tRNAs during peptidyl transfer
State 4a (S4a) of yeast 80S ribosome bound to 2 tRNAs and open eEF3 and eEF2 during translocation
State 3 (S3) of yeast 80S ribosome bound to 2 tRNAs during translocation
State 2b (S2b) of yeast 80S ribosome bound to 2 tRNAs during peptidyl transfer
Raw consensus map of type III CRISPR-associated deaminase in complex 2cA6 and 2ATP, partial activated
CryoEM structure of human GBRB3 at 2.1 angstrom resolution from Biortus
Structure of hemagglutinin from HN/4-10 H3N8 influenza virus G228 mutant complexed with human receptor analog LSTc
The structure of type III CRISPR-associated deaminase in complex 2cA6 and 2ATP, partial activated
State 6 (S6) of yeast 80S ribosome bound to 2 tRNAs and eEF2 and eEF3 during tranlocation
State 1a (S1a) of yeast 80S ribosome bound to open eEF3 and 2 tRNAs and eEF1A during mRNA decoding
30S-TEC (TEC in expressome position) Inactive state 2, focused TEC map
30S-TEC (TEC in expressome position) Inactive state 2, focused body map