Substrates for peptidase S01.162: kallikrein-related peptidase 3

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Pharma

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	Evidence	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference	CutDB
Abz-Asn-Lys-Ile-Ser-Tyr-Gln-Ser-Ser-Ser-Gln-EDDnp			Abz-Asn-Lys-Ile-Ser-Tyr-Gln+Ser-Ser-Ser-Gln-EDDnp	S		Ile	Ser	Tyr	Gln	Ser	Ser	Ser	Gln	Réhault et al., 2002
Abz-Ile-Ser-Tyr-Gln-Ser-Ser-Ser-Thr-Glu-Glu-Gln-EDDnp			Abz-Ile-Ser-Tyr+Gln-Ser-Ser-Ser-Thr-Glu-Glu-Gln-EDDnp	S		Abz	Ile	Ser	Tyr	Gln	Ser	Ser	Ser	Réhault et al., 2002
Abz-Ser-Ser-Gln-Tyr-Ser-Asn-Thr-Glu-Glu-Gln-EDDnp			Abz-Ser-Ser-Gln-Tyr+Ser-Asn-Thr-Glu-Glu-Gln-EDDnp	S		Ser	Ser	Gln	Tyr	Ser	Asn	Thr	Glu	Réhault et al., 2002
Abz-Ser-Ser-Ile-Tyr-Ser-Gln-Thr-Glu-Glu-Gln-EDDnp			Abz-Ser-Ser-Ile-Tyr+Ser-Gln-Thr-Glu-Glu-Gln-EDDnp	S		Ser	Ser	Ile	Tyr	Ser	Gln	Thr	Glu	Réhault et al., 2002
Ac-Gln-His-Tyr-Ser-Ser-Asn-NH2			Ac-Gln-His-Tyr+Ser-Ser-Asn-NH2	S		Ac	Gln	His	Tyr	Ser	Ser	Asn	NH2	Yang et al., 1999
Ac-Gln-Lys-Tyr-Ser-Ser-Asn-NH2			Ac-Gln-Lys-Tyr+Ser-Ser-Asn-NH2	S		Ac	Gln	Lys	Tyr	Ser	Ser	Asn	NH2	Yang et al., 1999
Ac-Gln-Phe-Tyr-Ser-Ser-Asn-NH2			Ac-Gln-Phe-Tyr+Ser-Ser-Asn-NH2	S		Ac	Gln	Phe	Tyr	Ser	Ser	Asn	NH2	Yang et al., 1999
Ac-Gln-Val-Tyr-Ser-Ser-Asn-NH2			Ac-Gln-Val-Tyr+Ser-Ser-Asn-NH2	S		Ac	Gln	Val	Tyr	Ser	Ser	Asn	NH2	Yang et al., 1999
alpha-1-peptidase inhibitor	P01009	25-418	peptide-Met382+Ser-peptide	P		Ala	Ile	Pro	Met	Ser	Ile	Pro	Pro	Zhang et al., 1997	20233
antileukoproteinase	P03973	26-132	peptide-Met98+Leu-peptide	P		Gln	Cys	Leu	Met	Leu	Asn	Pro	Pro	Ohlsson et al., 1995	3500
antileukoproteinase	P03973	26-132	peptide-Arg45+Tyr-peptide	P		Gln	Cys	Leu	Arg	Tyr	Lys	Lys	Pro	Ohlsson et al., 1995	3499
Bz-Arg-NHPhNO2			Bz-Arg+NHPhNO2	S		-	-	Bz	Arg	NAN	-	-	-	Watt et al., 1986
fibronectin	P02751	32-2386	peptide-Tyr294+Gln-peptide	P		Ala	Ala	Val	Tyr	Gln	Pro	Gln	Pro	Deperthes et al., 1996	20424
fibronectin	P02751	32-2386	peptide-Tyr881+Ala-peptide	P		Ile	Thr	Ile	Tyr	Ala	Val	Glu	Glu	Deperthes et al., 1996	20421
fibronectin	P02751	32-2386	peptide-Tyr1248+Thr-peptide	P		Val	Ser	Val	Tyr	Thr	Val	Lys	Asp	Deperthes et al., 1996	20422
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu+Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N		Asn	Gln	His	Leu	Cya	Gly	Ser	His	Akiyama et al., 1987
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr+Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N		Glu	Ala	Leu	Tyr	Leu	Val	Cya	Gly	Akiyama et al., 1987
insulin-like growth factor binding protein 3	P17936	28-291	peptide-Tyr186+Lys-peptide	P		Ser	Gln	Arg	Tyr	Lys	Val	Asp	Tyr	Okabe et al., 1999	3508
insulin-like growth factor binding protein 3	P17936	28-291	peptide-His158+Arg-peptide	P		Ser	Ser	Thr	His	Arg	Val	Ser	Asp	Okabe et al., 1999	3504
Kallikrein-1 precursor	P06870	19-262	peptide-Arg24+Ile-peptide	P	NT	Ile	Gln	Ser	Arg	Ile	Val	Gly	Gly	Yoon et al., 2007
Kallikrein-10 precursor	O43240	31-276	peptide-Arg42+Leu-peptide	P	NT	Asn	Asp	Thr	Arg	Leu	Asp	Pro	Glu	Yoon et al., 2007
Kallikrein-11 precursor	Q9UBX7	51-282	Glu-Thr-Arg53+Ile-peptide	P	NT	-	Glu	Thr	Arg	Ile	Ile	Lys	Gly	Yoon et al., 2007
Kallikrein-4 precursor	Q9Y5K2	27-254	Ser-Cys-Ser-Gln30+Ile-peptide	P	NT	Ser	Cys	Ser	Gln	Ile	Ile	Asn	Gly	Yoon et al., 2007
Kallikrein-5 precursor	Q9Y337	23-293	peptide-Arg66+Ile-peptide	P	NT	Ser	Ser	Ser	Arg	Ile	Ile	Asn	Gly	Yoon et al., 2007
Kallikrein-9 precursor	Q9UKQ9	16-250	peptide-Arg22+Ala-peptide	P	NT	Ala	Asp	Thr	Arg	Ala	Ile	Gly	Ala	Yoon et al., 2007
kallikrein-related peptidase 2	P20151	234-260	peptide-Tyr249+Arg-peptide	N		Val	Val	His	Tyr	Arg	Lys	Trp	Ile	Mikolajczyk et al., 1998
kallikrein-related peptidase 2	P20151	65-80	His-Cys-Leu-Lys-Lys-Asn-Ser-Gln-Val-Trp-Leu+Gly-Arg-His-Asn-Leu	N		Gln	Val	Trp	Leu	Gly	Arg	His	Asn	Mikolajczyk et al., 1998
kallikrein-related peptidase 2, prostatic isoform 1	P20151	18-31	peptide-Arg24+Ile-peptide	N		Ile	Gln	Ser	Arg	Ile	Val	Gly	Gly	Mikolajczyk et al., 1998	21007
leukocyte elastase inhibitor	P30740	1-379	peptide-Cys344+Met-peptide	P		Ala	Thr	Phe	Cys	Met	Leu	Met	Pro	Cooley et al., 2001	20489
Mca-Gln-Phe-Tyr-Ser-Ser-Asn-Lyd-NH2			Mca-Gln-Phe-Tyr+Ser-Ser-Asn-Lyd-NH2	S		Mca	Gln	Phe	Tyr	Ser	Ser	Asn	Lyd	Yang et al., 1999
morpholinecarbonyl-Ala-Thr-Lys-Ser-Lys-Gln-His-NHMec			morpholinecarbonyl-Ala-Thr-Lys-Ser-Lys-Gln-His+NHMec	S		Ser	Lys	Gln	His	AMC	-	-	-	Denmeade et al., 1997
morpholinecarbonyl-Gln-Asn-Lys-Ile-Ser-Tyr-Gln-NHMec			morpholinecarbonyl-Gln-Asn-Lys-Ile-Ser-Tyr-Gln+NHMec	S		Ile	Ser	Tyr	Gln	AMC	-	-	-	Denmeade et al., 1997
morpholinecarbonyl-Glu-Asn-Lys-Ile-Ser-Tyr-Gln-NHMec			morpholinecarbonyl-Glu-Asn-Lys-Ile-Ser-Tyr-Gln+NHMec	S		Ile	Ser	Tyr	Gln	AMC	-	-	-	Denmeade et al., 1997
morpholinecarbonyl-Glu-His-Ser-Ser-Lys-Leu-Gln-NHMec			morpholinecarbonyl-Glu-His-Ser-Ser-Lys-Leu-Gln+NHMec	S		Ser	Lys	Leu	Gln	AMC	-	-	-	Denmeade et al., 1997
morpholinecarbonyl-Gly-Gln-Lys-Gly-Gln-His-Tyr-AMC			morpholinecarbonyl-Gly-Gln-Lys-Gly-Gln-His-Tyr+AMC	S		Gly	Gln	His	Tyr	AMC	-	-	-	Denmeade et al., 1997
morpholinecarbonyl-His-Ser-Ser-Lys-Leu-Gln-NHMec			morpholinecarbonyl-His-Ser-Ser-Lys-Leu-Gln+NHMec	S		Ser	Lys	Leu	Gln	AMC	-	-	-	Denmeade et al., 1997
morpholinecarbonyl-Leu-Gly-Gly-Ser-Ser-Gln-Leu-NHMec			morpholinecarbonyl-Leu-Gly-Gly-Ser-Ser-Gln-Leu+NHMec	S		Ser	Ser	Gln	Leu	AMC	-	-	-	Denmeade et al., 1997
morpholinecarbonyl-Lys-Gly-Ile-Ser-Ser-Gln-Tyr-NHMec			morpholinecarbonyl-Lys-Gly-Ile-Ser-Ser-Gln-Tyr+NHMec	S		Ser	Ser	Gln	Tyr	AMC	-	-	-	Denmeade et al., 1997
morpholinecarbonyl-Lys-Gly-Leu-Ser-Ser-Gln-Cys-NHMec			morpholinecarbonyl-Lys-Gly-Leu-Ser-Ser-Gln-Cys+NHMec	S		Ser	Ser	Gln	Cys	AMC	-	-	-	Denmeade et al., 1997
morpholinecarbonyl-Ser-Arg-Lys-Ser-Gln-Gln-Tyr-NHMec			morpholinecarbonyl-Ser-Arg-Lys-Ser-Gln-Gln-Tyr+NHMec	S		Ser	Gln	Gln	Tyr	AMC	-	-	-	Denmeade et al., 1997
morpholinecarbonyl-Ser-Lys-Leu-Gln-NHMec			morpholinecarbonyl-Ser-Lys-Leu-Gln+NHMec	S		Ser	Lys	Leu	Gln	AMC	-	-	-	Denmeade et al., 1997
Parathyroid hormone-related protein	P12272	37-177	peptide-Phe59+Leu-peptide	P		Arg	Arg	Phe	Phe	Leu	His	His	Leu	Cramer et al., 1996	3432
plasminogen	P00747	20-810	peptide-Glu458+Ala-peptide	P		Ser	Gly	Thr	Glu	Ala	Ser	Val	Val	Heidtmann et al., 1999	3513
Prostate-specific antigen precursor	P07288	18-261	peptide-Arg24+Ile-peptide	P	NT	Ile	Leu	Ser	Arg	Ile	Val	Gly	Gly	Yoon et al., 2007
S-2504			Bz-Lys-Val-Tyr-+NHPhNO2	S		Bz	Lys	Val	Tyr	NAN	-	-	-	Christensson et al., 1990
S-2586			MeO-Suc-Arg-Pro-Tyr+NHPhNO2	S		Suc	Arg	Pro	Tyr	NAN	-	-	-	Christensson et al., 1990
semenogelin-1	P04279	24-462	peptide-Leu107+His-peptide	P	NT	Gln	Gln	Leu	Leu	His	Asn	Lys	Gln	Malm et al., 2000	3463
semenogelin-1	P04279	24-462	peptide-Tyr67+Thr-peptide	P	NT	Ser	Ile	Gln	Tyr	Thr	Tyr	His	Val	Malm et al., 2000	3462
semenogelin-1	P04279	24-462	peptide-Asn412+Arg-peptide	P	NT	Gln	Ser	Thr	Asn	Arg	Glu	Gln	Asp	Malm et al., 2000	3461
semenogelin-1	P04279	24-462	peptide-Tyr375+Ser-peptide	P	NT	Arg	Ser	Ile	Tyr	Ser	Gln	Thr	Glu	Malm et al., 2000	3459
semenogelin-1	P04279	24-462	peptide-Asp368+Val-peptide	P	NT	Val	Gln	Lys	Asp	Val	Ser	Gln	Arg	Malm et al., 2000	3458
semenogelin-1	P04279	24-462	peptide-Gln335+Glu-peptide	P	NT	Ile	Pro	Ser	Gln	Glu	Gln	Glu	His	Malm et al., 2000	3457
semenogelin-1	P04279	24-462	peptide-Asp308+Val-peptide	P	NT	Val	Gln	Lys	Asp	Val	Ser	Gln	Ser	Malm et al., 2000	3456
semenogelin-1	P04279	24-462	peptide-Tyr360+Gly-peptide	P	NT	Arg	Leu	His	Tyr	Gly	Glu	Asn	Gly	Malm et al., 2000
semenogelin-1	P04279	24-462	peptide-Gln349+Ser-peptide	P	NT	Ile	Ser	Tyr	Gln	Ser	Ser	Ser	Thr	Malm et al., 2000
semenogelin-1	P04279	24-462	peptide-Tyr315+Ser-peptide	P	NT	Ser	Ser	Ile	Tyr	Ser	Gln	Thr	Glu	Malm et al., 2000	3449
semenogelin-1	P04279	24-462	peptide-Tyr300+Gly-peptide	P	NT	Arg	Leu	His	Tyr	Gly	Glu	Asn	Gly	Malm et al., 2000	3447
semenogelin-1	P04279	24-462	peptide-Gln289+Ser-peptide	P	NT	Ile	Ser	Tyr	Gln	Ser	Ser	Ser	Thr	Malm et al., 2000
semenogelin-1	P04279	24-462	peptide-Tyr263+Asn-peptide	P	NT	Leu	Leu	Val	Tyr	Asn	Lys	Asn	Gln	Malm et al., 2000	3444
semenogelin-1	P04279	24-462	peptide-Gln235+Thr-peptide	P	NT	Ser	Lys	Val	Gln	Thr	Ser	Leu	Cys	Malm et al., 2000	3442
semenogelin-1	P04279	24-462	peptide-Gln221+Asn-peptide	P	NT	Gly	His	Tyr	Gln	Asn	Val	Val	Glu	Malm et al., 2000	3441
semenogelin-1	P04279	24-462	peptide-Tyr159+Ser-peptide	P	NT	Ser	Ser	Gln	Tyr	Ser	Asn	Thr	Glu	Malm et al., 2000	3439
semenogelin-1	P04279	24-462	peptide-Tyr85+Asp-peptide	P	NT	Ser	Gln	Gln	Tyr	Asp	Leu	Asn	Ala	Malm et al., 2000	3437
semenogelin-1	P04279	24-462	peptide-Tyr48+Ser-peptide	P	NT	Gly	Gln	His	Tyr	Ser	Gly	Gln	Lys	Malm et al., 2000	3434
semenogelin-2	Q02383	24-582	peptide-Tyr480+Gly-peptide	P	NT	Arg	Leu	Asn	Tyr	Gly	Gly	Lys	Ser	Malm et al., 2000	3498
semenogelin-2	Q02383	24-582	peptide-Asn419+Ser-peptide	P	NT	Arg	Arg	Leu	Asn	Ser	Gly	Glu	Lys	Malm et al., 2000	3497
semenogelin-2	Q02383	24-582	peptide-Gln395+Ala-peptide	P	NT	Ile	Pro	Ser	Gln	Ala	Gln	Glu	Tyr	Malm et al., 2000	3496
semenogelin-2	Q02383	24-582	peptide-Gln329+Val-peptide	P	NT	Ser	Gln	Asn	Gln	Val	Thr	Ile	His	Malm et al., 2000	3493
semenogelin-2	Q02383	24-582	peptide-Tyr194+Val-peptide	P	NT	Gln	Ser	Ser	Tyr	Val	Leu	Gln	Thr	Malm et al., 2000
semenogelin-2	Q02383	24-582	peptide-Gln235+Thr-peptide	P	NT	Ser	Lys	Leu	Gln	Thr	Ser	Leu	His	Malm et al., 2000	3490
semenogelin-2	Q02383	24-582	peptide-Gln221+Asn-peptide	P	NT	Gly	His	Tyr	Gln	Asn	Val	Val	Asp	Malm et al., 2000	3489
semenogelin-2	Q02383	24-582	peptide-His99+Leu-peptide	P	NT	Ser	Lys	Gln	His	Leu	Gly	Gly	Ser	Malm et al., 2000	3488
semenogelin-2	Q02383	24-582	peptide-Tyr85+Asp-peptide	P	NT	Ser	Gln	Gln	Tyr	Asp	Leu	Asn	Ala	Malm et al., 2000
semenogelin-2	Q02383	24-582	peptide-Gln469+Ser-peptide	P	NT	Met	Ser	Tyr	Gln	Ser	Ser	Ser	Thr	Malm et al., 2000	3483
semenogelin-2	Q02383	24-582	peptide-Gln409+Ser-peptide	P	NT	Ile	Ser	Tyr	Gln	Ser	Ser	Ser	Thr	Malm et al., 2000
semenogelin-2	Q02383	24-582	peptide-Tyr48+Phe-peptide	P	NT	Gly	Gln	His	Tyr	Phe	Gly	Gln	Lys	Malm et al., 2000
semenogelin-2	Q02383	24-582	peptide-Gln349+Ser-peptide	P	NT	Ile	Ser	Tyr	Gln	Ser	Ser	Ser	Thr	Malm et al., 2000	3445
semenogelin-2	Q02383	24-582	peptide-His67+Thr-peptide	P	NT	Ser	Ile	Gln	His	Thr	Tyr	His	Val	Malm et al., 2000
semenogelin-2	Q02383	24-582	peptide-His299+His-peptide	P	NT	Arg	Gln	Leu	His	His	Gly	Glu	Lys	Malm et al., 2000
semenogelin-2	Q02383	24-582	peptide-His242+Gln-peptide	P	NT	His	Pro	Ala	His	Gln	Asp	Arg	Leu	Malm et al., 2000
Suc-Ala-Ala-Pro-Phe-NHPhNO2			Suc-Ala-Ala-Pro-Phe+NHPhNO2	S		Ala	Ala	Pro	Phe	NAN	-	-	-	Watt et al., 1986
Suc-Gly-Pro-Lys-NHPhNO2			Suc-Gly-Pro-Lys+NHPhNO2	S		Suc	Gly	Pro	Lys	NAN	-	-	-	Waheed et al., 2008
urokinase-type plasminogen activator	P00749	21-431	peptide-Lys158+Ile-peptide	P		Pro	Arg	Phe	Lys	Ile	Ile	Gly	Gly		17297
vascular endothelial growth factor C	P49767	32-419	peptide-Tyr114+Asn-peptide	P	NT	Ala	Ala	His	Tyr	Asn	Thr	Glu	Ile	Kunnapuu et al., 2021
vascular endothelial growth factor D	O43915	22-354	peptide-Tyr94+Asp-peptide	P	NT	Ala	Thr	Phe	Tyr	Asp	Ile	Glu	Thr	Kunnapuu et al., 2021
vascular endothelial growth factor D	O43915	22-354	peptide-Tyr200+Ser-peptide	P	NT	Arg	His	Pro	Tyr	Ser	Ile	Ile	Arg	Kunnapuu et al., 2021