Substrates for peptidase M13.008: neprilysin-2

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Literature Substrates

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	Evidence	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference	CutDB
Angiotensin-1	P01019	34-43	Asp-Arg-Val-Tyr37+Ile-Peptide	N	NT	Asp	Arg	Val	Tyr	Ile	His	Pro	Phe	Rose et al., 2002
Angiotensin-1	P01019	34-43	Peptide-Pro40+Phe-His-Leu	N	NT	Tyr	Ile	His	Pro	Phe	His	Leu	-	Rose et al., 2002
Atrial natriuretic factor	P01161	123-150	Peptide-Ser147+Phe-Arg-Tyr	N	NT	Gly	Cys	Asn	Ser	Phe	Arg	Tyr	-	Rose et al., 2002
Beta-amyloid protein 40	P05067	672-711	peptide-Lys687+Leu-peptide	P	MS	His	His	Gln	Lys	Leu	Val	Phe	Phe	Huang et al., 2008
Beta-amyloid protein 40	P05067	672-711	peptide-Leu688+Val-peptide	P	MS	His	Gln	Lys	Leu	Val	Phe	Phe	Ala	Huang et al., 2008
Beta-amyloid protein 42	P05067	672-713	peptide-Lys687+Leu-peptide	P	MS	His	His	Gln	Lys	Leu	Val	Phe	Phe	Huang et al., 2008
Beta-amyloid protein 42	P05067	672-713	peptide-Leu688+Val-peptide	P	MS	His	Gln	Lys	Leu	Val	Phe	Phe	Ala	Huang et al., 2008
big endothelin 1	P05305	53-90	peptide-Trp21+Val22-peptide	P		Asp	Ile	Ile	Trp	Val	Asn	Thr	Pro	Carpentier et al., 2003	17027
Bradykinin	P01042	381-389	Peptide-Pro387+Phe-Arg	N	NT	Gly	Phe	Ser	Pro	Phe	Arg	-	-	Rose et al., 2002
Cholecystokinin-8	P06307	96-103	Asp-Tyr-Met-Gly99+Trp-Met-Asp-Phe	N	NT	Asp	Tyr	Met	Gly	Trp	Met	Asp	Phe	Rose et al., 2002
Gonadoliberin-1	P01148	24-33	Peptide-Gly29+Leu-Arg-Pro-Gly	N	NT	Trp	Ser	Tyr	Gly	Leu	Arg	Pro	Gly	Rose et al., 2002
Leu-enkephalin	P01210	230-234	Tyr-Gly-Gly232+Phe-Leu	N	NT	-	Tyr	Gly	Gly	Phe	Leu	-	-	Rose et al., 2002
Met-enkephalin	P01210	100-104	Tyr-Gly-Gly102+Phe-Met	N	NT	-	Tyr	Gly	Gly	Phe	Met	-	-	Rose et al., 2002
Substance P	P20366	58-68	Peptide-Gln63+Phe-Peptide	N	NT	Lys	Pro	Gln	Gln	Phe	Phe	Gly	Leu	Rose et al., 2002
Substance P	P20366	58-68	Peptide-Gly66+Leu-Met	N	NT	Gln	Phe	Phe	Gly	Leu	Met	NH2	-	Rose et al., 2002
[Leu5]-enkephalin	P01213	175-179	Tyr-Gly-Gly+Phe-Leu	N		-	Tyr	Gly	Gly	Phe	Leu	-	-	Carpentier et al., 2003