Substrates for peptidase M12.005: procollagen C-peptidase

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Pharma

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	Evidence	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference	CutDB
alpha-2-macroglobulin	P01023	24-1474	peptide-Ser710+Asp-peptide	P		Phe	Tyr	Glu	Ser	Asp	Val	Met	Gly	Zhang et al., 2006
apolipoprotein A-I	P02647	21-28	peptide-Gln24+Asp-peptide	P	CS	Phe	Trp	Gln	Gln	Asp	Glu	Pro	Pro	Chau et al., 2007
basement membrane-specific heparan sulfate proteoglycan core protein	P98160	3687-4391	peptide-Asn4196+Asp-peptide	P	NT	Ser	Gly	Gly	Asn	Asp	Ala	Pro	Gly	Gonzalez et al., 2005
biglycan	P28653	20-369	peptide-Asn37+Asp-peptide	P	NT	Leu	Met	Met	Asn	Asp	Glu	Glu	Ala	Scott et al., 2000
chordin precursor	Q9P0Z4	27-955	peptide-Ser127+Asp-peptide	P		Arg	Ser	Tyr	Ser	Asp	Arg	Gly	Glu	Scott et al., 2000	16689
chordin precursor	Q9P0Z4	27-955	peptide-Ala840+Asp-peptide	P		Pro	Met	Gln	Ala	Asp	Gly	Pro	Arg	Scott et al., 2000	16688
collagen alpha 1(I) precursor	Q15201	162-1218	peptide-Ala1196+Asp-peptide	P		Tyr	Tyr	Arg	Ala	Asp	Asp	Ala	Asn	Kessler, 2004
collagen alpha 1(II) chain precursor	P02460	1-369	peptide-Ala123+Asp-peptide	N		Tyr	Met	Arg	Ala	Asp	Glu	Ala	Ala		16690
collagen alpha 1(VII) precursor	Q02388	17-2944	peptide-Ala2821+Asp-peptide	P		Ser	Tyr	Ala	Ala	Asp	Thr	Ala	Gly	Rattenholl et al., 2002	3061
collagen alpha 2(I) chain precursor	P08123	25-1366	peptide-Ala1119+Asp-peptide	P		Phe	Tyr	Arg	Ala	Asp	Gln	Pro	Arg	Scott et al., 2000	16699
collagen alpha-1(I) chain	P02452	23-1464	peptide-Ala1218+Asp-peptide	P		Tyr	Tyr	Arg	Ala	Asp	Asp	Ala	Asn	Kofford et al., 1997	16693
collagen alpha-1(II) chain	P02458	182-1241	peptide-Ala1147+Asp-peptide	P		Tyr	Met	Arg	Ala	Asp	Gln	Ala	Ala	Kessler, 2004	16694
collagen alpha-1(III) chain	P02461	24-1466	peptide-Gly1198+Asp-peptide	P		Pro	Tyr	Tyr	Gly	Asp	Glu	Pro	Met	Scott et al., 2000	16695
collagen alpha-1(V) chain	P20908	38-1605	peptide-Ser253+Gln-peptide	P		Thr	Pro	Gln	Ser	Gln	Asp	Pro	Asn	Bonod-Bidaud et al., 2007	16697
collagen alpha-1(V) chain	P20908	38-1605	peptide-Asp1594+Asp-peptide	P		Gln	Leu	Leu	Asp	Asp	Gly	Asn	Gly	Unsöld et al., 2002	3062
collagen alpha-1(XI) chain	P12107	37-1806	peptide-Ala253+Gln-peptide	P	NT	Ala	Ala	Gln	Ala	Gln	Glu	Pro	Gln	Medeck et al., 2003
collagen alpha-2(V) chain precursor	P05997	27-1499	peptide-Glu1250+Asp-peptide	P		Glu	Phe	Thr	Glu	Asp	Gln	Ala	Ala	Unsöld et al., 2002	3063
collagen alpha-2(XI) chain	P13942	23-1500	peptide-Asn238+Gln-peptide	P	NT	Arg	Pro	Gln	Asn	Gln	Gln	Pro	His	Medeck et al., 2003
collagen alpha-3(V) chain	P25940	30-1745	peptide-Gln463+Ala-peptide	P	NT	Ser	Phe	Gln	Gln	Ala	Gln	Ala	Gln	Gopalakrishnan et al., 2004
decorin precursor	P07585	17-359	peptide-Glu14+Asp-peptide	P		Phe	Met	Leu	Glu	Asp	Glu	Ala	Ser	Scott et al., 2000	16701
dentin matrix acidic phosphoprotein 1 precursor	O55188	17-503	peptide-Ser212+Asp-peptide	P		Gly	Met	Gln	Ser	Asp	Asp	Pro	Glu	Ge et al., 2005	19753
DSPP600	Q6YBV4	463-476	Tyr-Glu-Phe-Asp-Gly-Lys-Ser-Met-Gln-Gly+Asp-Asp-Pro-Asn	P	NT	Ser	Met	Gln	Gly	Asp	Asp	Pro	Asn	Tsuchiya et al., 2010
DSPP600	Q6YBV4	1-600	peptide-Gly472+Asp-peptide	P	NT	Ser	Met	Gln	Gly	Asp	Asp	Pro	Asn	Tsuchiya et al., 2010
gldn gliomedin	Q8BMF8	1-549	peptide-Asn277+Asp-peptide	P		Val	Ile	Pro	Asn	Asp	Asp	Thr	Leu	Maertens et al., 2007	25802
growth/differentiation factor 11 precursor	Q9Z1W4	21-405	peptide-Gly119+Asp-peptide	P		Asp	Phe	Gln	Gly	Asp	Ala	Leu	Gln	Ge et al., 2005
growth/differentiation factor 8 precursor	O08689	25-376	peptide-Arg99+Asp-peptide	P		Asp	Val	Gln	Arg	Asp	Asp	Ser	Ser	Wolfman et al., 2003
insulin-like growth factor-binding protein 3	P17936	28-291	peptide-Ser194+Thr-peptide	P	NT	Glu	Ser	Gln	Ser	Thr	Asp	Thr	Gln	Kim et al., 2011
insulin-like growth factor-binding protein 3	P47878	28-291	peptide-Gln193+Ser-peptide	P	NT	Tyr	Glu	Ser	Gln	Ser	Thr	Asp	Thr	Kim et al., 2011
laminin subunit gamma-2	Q61092	22-1191	peptide-Gly434+Asp-peptide	P		Cys	Tyr	Ser	Gly	Asp	Glu	Asn	Pro	Veitch et al., 2003	19540
latent-transforming growth factor beta-binding protein 1	Q14766	24-1721	peptide-Leu811+Asp-peptide	P	NT	Ile	Pro	Ser	Leu	Asp	Gln	Glu	Lys	Ge & Greenspan, 2006
latent-transforming growth factor beta-binding protein 1	Q14766	24-1721	peptide-Gln1610+Asp-peptide	P	NT	Tyr	Phe	Ile	Gln	Asp	Arg	Phe	Leu	Ge & Greenspan, 2006
lysyl oxidase homolog 1	Q08397	95-574	peptide-His151+Gly-peptide	P	MS	Gln	Gln	Arg	His	Gly	Gly	Ser	Ala	Rosell-Garcia et al., 2022
Lysyl oxidase homolog 3	P58215	26-753	peptide-Gly447+Asp-peptide	P		Leu	Ile	Cys	Gly	Asp	Asp	Trp	Gly	Lee & Kim, 2006
Lysyl oxidase-like 1	A0JNB6	1-592	peptide-Gly131+Asp-peptide	P	NT	Val	Ala	Val	Gly	Asp	Ser	Thr	Gly	Borel et al., 2001
Lysyl oxidase-like 1	A0JNB6	1-592	peptide-Ser355+Asp-peptide	P	NT	Val	Arg	Ser	Ser	Asp	Ala	Pro	Pro	Borel et al., 2001
osteoglycin	Q543C5	1-298	peptide-Lys75+Asp-peptide	P	NT	Gln	Leu	Gln	Lys	Asp	Glu	Val	Ile	Ge et al., 2004
probiglycan	P21810	20-368	peptide-Asn18+Asp-peptide	P		Phe	Met	Met	Asn	Asp	Glu	Glu	Ala	Scott et al., 2000	16692
Prolactin	P01236	29-227	peptide-Ala187+Asp-peptide	P	NT	Leu	Gln	Met	Ala	Asp	Glu	Glu	Ser	Ge et al., 2007
prolysyl oxidase	P16636	22-411	peptide-Gly162+Asp-peptide	P		Arg	Met	Val	Gly	Asp	Asp	Pro	Tyr	Kessler, 2004	16703
prolysyl oxidase	P28300	22-417	peptide-Gly169+Asp-peptide	P		Gly	Met	Val	Gly	Asp	Asp	Pro	Tyr	Uzel et al., 2001	16702
prolysyl oxidase-like 1		92-591	peptide-Gly40+Asp-peptide	N		Val	Ala	Val	Gly	Asp	Ser	Thr	Gly	Kessler, 2004	16704
Somatotropin	P01241	27-217	peptide-Asn178+Asp-peptide	P	NT	Asn	Ser	His	Asn	Asp	Asp	Ala	Leu	Ge et al., 2007