Substrates for peptidase M13.002: endothelin-converting enzyme 1

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Inhibitors Pharma

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate Uniprot Residue range Cleavage Site Cleavage type Evidence P4 P3 P2 P1 P1' P2' P3' P4' Reference CutDB
angiotensin-1 P01019 34-43 Asp-Arg-Val-Tyr-Ile-His-Pro+Phe-His-Leu N Tyr Ile His Pro Phe His Leu Val Carson et al., 2002 24223
angiotensin-1 P01019 34-43 Asp-Arg-Val-Tyr+Ile-His-Pro-Phe-His-Leu N Asp Arg Val Tyr Ile His Pro Phe Carson et al., 2002 24224
angiotensin-1 P01019 34-43 Asp-Arg-Val+Tyr-Ile-His-Pro-Phe-His-Leu N - Asp Arg Val Tyr Ile His Pro Carson et al., 2002 24225
beta-amyloid protein 42 P05067 672-713 peptide-Glu674+Phe-peptide P - Asp Ala Glu Phe Arg His Asp Yan et al., 2006
beta-amyloid protein 42 P05067 672-713 peptide-Lys687+Leu-peptide P His His Gln Lys Leu Val Phe Phe Yan et al., 2006
beta-amyloid protein 42 P05067 672-713 peptide-Leu688+Val-peptide P His Gln Lys Leu Val Phe Phe Ala Yan et al., 2006
beta-amyloid protein 42 P05067 672-713 peptide-Phe690+Phe-peptide P Lys Leu Val Phe Phe Ala Glu Asp Yan et al., 2006
big endothelin 1 P05305 53-90 peptide-Trp21+Val22-peptide P Asp Ile Ile Trp Val Asn Thr Pro Ahn & Johnson, 2004
big endothelin 1 P05305 53-90 peptide-His79+Val-peptide P Thr Pro Glu His Val Val Pro Tyr Fahnoe et al., 2000 3735
big endothelin 1 P05305 53-90 peptide-Gly86+Ser-peptide P Tyr Gly Leu Gly Ser Pro Arg Ser Fahnoe et al., 2000 3736
big endothelin 3 precursor P14138 17-238 peptide-Trp21+Ile22-peptide P Asp Ile Ile Trp Ile Asn Thr Pro Ahn & Johnson, 2004 17020
bradykinin P01042 381-389 Arg-Pro-Pro-Gly-Phe-Ser-Pro+Phe-Arg N Gly Phe Ser Pro Phe Arg - - Ahn & Johnson, 2004 17021
endothelin-1 P05305 53-73 peptide-Asp70+Ile-peptide P Cys His Leu Asp Ile Ile Trp Val Fahnoe et al., 2000 3733
endothelin-1 P05305 53-73 peptide-Ile71+Ile-peptide P His Leu Asp Ile Ile Trp Val Asn Fahnoe et al., 2000 3734
endothelin-1 P05305 53-73 peptide-Tyr65+Phe-peptide P Glu Cys Val Tyr Phe Cys His Leu Fahnoe et al., 2000 3731
endothelin-1 P05305 53-73 peptide-Phe66+Cys-peptide P Cys Val Tyr Phe Cys His Leu Asp Fahnoe et al., 2000 3732
endothelin-1 P05305 53-73 peptide-Ser57+Leu-peptide P Ser Cys Ser Ser Leu Met Asp Lys Fahnoe et al., 2000 3730
endothelin-1 precursor Q6FH53 17-212 peptide-Arg92+Ala-peptide P Arg Ser Lys Arg Ala Leu Glu Asn Kido et al., 1998 3729
endothelin-1 precursor Q6FH53 17-212 peptide-Arg52+Cys-peptide P Arg Ser Lys Arg Cys Ser Cys Ser Kido et al., 1998 3728
endothelin-2 precursor P20800 25-178 peptide-Trp21+Val22-peptide P Asp Ile Ile Trp Val Asn Thr Pro Fahnoe et al., 2000 17019
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His+Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N NT Cya Gly Ser His Leu Val Glu Ala Johnson et al., 1999
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu+Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N NT Gly Ser His Leu Val Glu Ala Leu Johnson et al., 1999
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu+Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N NT His Leu Val Glu Ala Leu Tyr Leu Johnson et al., 1999
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala+Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N NT Leu Val Glu Ala Leu Tyr Leu Val Johnson et al., 1999
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu+Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N NT Val Glu Ala Leu Tyr Leu Val Cya Johnson et al., 1999
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr+Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N NT Glu Ala Leu Tyr Leu Val Cya Gly Johnson et al., 1999
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr+Pro-Lys-Ala N NT Phe Phe Tyr Thr Pro Lys Ala - Johnson et al., 1999
KNG1 kininogen 1 isoform 1 Q6PAU9 1-644 peptide-Pro387+Phe-peptide P Gly Phe Ser Pro Phe Arg Ser Ser 24192
Mca-Arg-Pro-Pro-Gly-Phe-Ser-Ala+Phe-Lys-Dnp Mca-Arg-Pro-Pro-Gly-Phe-Ser-Ala+Phe-Lys-Dnp S Gly Phe Ser Ala Phe Lys DNP - Ahn & Johnson, 2004
neurotensin P30990 151-163 Glp-Leu+Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu N - - Glp Leu Tyr Glu Asn Lys Carson et al., 2002 24220
neurotensin P30990 151-163 Glp-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro+Tyr-Ile-Leu N Pro Arg Arg Pro Tyr Ile Leu - Ahn & Johnson, 2004 17023
substance P P20366 58-68 Arg-Pro-Lys-Pro-Gln-Gln+Phe-Phe-Gly-Leu-Met-NH2 N Lys Pro Gln Gln Phe Phe Gly Leu Carson et al., 2002 17024
substance P P20366 58-68 Arg-Pro-Lys-Pro-Gln-Gln-Phe+Phe-Gly-Leu-Met-NH2 N Pro Gln Gln Phe Phe Gly Leu Met Ahn & Johnson, 2004 17025
substance P P20366 58-68 Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly+Leu-Met-NH2 N Gln Phe Phe Gly Leu Met NH2 - Carson et al., 2002 24209