Substrates for peptidase S09.003: dipeptidyl-peptidase IV (eukaryote)

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Pharma

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	Evidence	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference	CutDB
Ala-Pro-NHPhNO2			Ala-Pro+NHPhNO2	S		-	-	Ala	Pro	NAN	-	-	-	Bjelke et al., 2006
aminoacylalanyl bond			Xaa-Ala+peptide	N		-	-	Xaa	Ala	CPe	-	-	-	Misumi & Ikehara, 2004
aminoacylhydroxyprolyl bond			Xaa-Hyp+peptide	N		-	-	Xaa	Hyp	CPe	-	-	-	Misumi & Ikehara, 2004
aminoacylprolyl bond			Xaa-Pro+peptide	N		-	-	Xaa	Pro	CPe	-	-	-	Misumi & Ikehara, 2004
Arg-Pro-NHPhNO2			Arg-Pro+NHPhNO2	S		-	-	Arg	Pro	NAN	-	-	-	Bjelke et al., 2006
C-C motif chemokine 2	P13500	24-31	Gln-Pro+Asp-Ala-Ile-Asn-Ala-Pro	N		-	-	Gln	Pro	Asp	Ala	Ile	Asn	Kumagai et al., 2000
C-X-C motif chemokine 10	P02778	22-98	Val-Pro23+Leu-peptide	P		-	-	Val	Pro	Leu	Ser	Arg	Thr	Lambeir et al., 2001	3642
C-X-C motif chemokine 11	O14625	22-94	Phe-Pro23+Met-peptide	P		-	-	Phe	Pro	Met	Phe	Lys	Arg	Lambeir et al., 2001	3641
C-X-C motif chemokine 9	Q07325	23-125	Thr-Pro24+Val-peptide	P		-	-	Thr	Pro	Val	Val	Arg	Lys	Lambeir et al., 2001	3643
Calcitonin gene-related peptide 1	P06881	83-119	Ala-Cys84+Asp-peptide	P	-	-	-	Ala	Cys	Asp	Thr	Ala	Thr	Sedo et al., 2005
eotaxin	P51671	24-97	Gly-Pro25+Ala-peptide	P		-	-	Gly	Pro	Ala	Ser	Val	Pro	Lambeir et al., 2001	3644
gastric inhibitory polypeptide (GIP)			Tyr-Ala+peptide	P		-	-	Tyr	Ala	Glu	Gly	Thr	Phe	Kieffer et al., 1995
Gastrin-releasing peptide	P07492	24-50	Val-Pro25+Leu-peptide	P	-	-	-	Val	Pro	Leu	Pro	Ala	Gly	Sedo et al., 2005
glucagon-like peptide 1	P01274	92-128	His-Asp+Glu-peptide	P		-	-	His	Asp	Glu	Phe	Glu	Arg	Kieffer et al., 1995
glucagon-like peptide 1 amide	P01275	98-127	Arg+Ala-peptide	P	NT	'-'	'-'	His	Ala	Glu	Gly	Thr	Phe	Gao et al., 2021
Gly-Pro-NHMec			Gly-Pro+NHMec	S		-	-	Gly	Pro	AMC	-	-	-
Gly-Pro-NHNap			Gly-Pro+NHNap	S		-	-	Gly	Pro	NAP	-	-	-	Nakajima et al., 2008
Gly-Pro-NHPhN02			Gly-Pro+NHPhN02	S		-	-	Gly	Pro	NAN	-	-	-	Misumi & Ikehara, 2004
macrophage inflammatory protein 2-alpha precursor	P19875	35-107	Ala-Pro36+Leu-peptide	P		-	-	Ala	Pro	Leu	Ala	Thr	Glu	Ajami et al., 2008
major prion protein (Gly>Pro,Trp>Phe mutant)	P04925	23-230	peptide-Gly62+Gly-peptide	N	NT	Pro	His	Gly	Pro	Gly	Phe	Gly	Gln
major prion protein (Gly>Pro,Trp>Phe mutant)	P04925	23-230	peptide-Gly70+Ser-peptide	N	NT	Pro	His	Gly	Pro	Ser	Phe	Gly	Gln
major prion protein (Gly>Pro,Trp>Phe mutant)	P04925	23-230	peptide-Gly78+Ser-peptide	N	NT	Pro	His	Gly	Pro	Ser	Phe	Gly	Gln
major prion protein (Gly>Pro,Trp>Phe mutant)	P04925	23-230	peptide-Gly86+Gly-peptide	N	NT	Pro	His	Gly	Pro	Gly	Phe	Gly	Gln
major prion protein (Gly>Pro,Trp>Phe mutant)	P04925	23-230	peptide-Pro39+Gly-peptide	N	NT	Ser	Arg	Tyr	Pro	Gly	Gln	Gly	Ser
major prion protein (Gly>Pro,Trp>Phe mutant)	P04925	23-230	peptide-Gly92+Gly-peptide	N	NT	Gly	Gln	Gly	Gly	Gly	Thr	His	Asn
Melanotropin beta	P01189	219-234	Asp-Glu-Gly-Pro220+Tyr-peptide	P	NT	-	-	Gly	Pro	Tyr	Arg	Met	Glu	Hsiung et al., 2005
neuropeptide Y	P01303	29-36	Tyr-Pro+Ser-Ser-Leu-Lys-Pro-Asp-Asn-Pro-Gly-Gly-Glu-Asp-Ala-Pro-Ala-Gly-Glu-Asp-Met-Ala-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Leu-Asn-Leu-Ile-Thr-Arg-Gln-Arg-Tyr-NH2	P		-	-	Tyr	Pro	Ser	Leu	Lys	Pro	Abid et al., 2009
small inducible cytokine A22	O00626	25-93	Gly-Pro26+Tyr-peptide	P		-	-	Gly	Pro	Tyr	Gly	Ala	Asn	Lambeir et al., 2001	3639
small inducible cytokine A22	O00626	27-93	Tyr-Gly28+Ala-peptide	P		-	-	Tyr	Gly	Ala	Asn	Met	Glu	Lambeir et al., 2001	3640
small inducible cytokine A3-like 1	P16619	24-93	Ala-Pro25+Leu-peptide	P		-	-	Ala	Pro	Leu	Ala	Ala	Asp	Lambeir et al., 2001	3646
small inducible cytokine A5 precursor	P13501	24-91	Ser-Pro25+Tyr-peptide	P		-	-	Ser	Pro	Tyr	Ser	Ser	Asp	Lambeir et al., 2001	3645
small inducible cytokine B6	P80162	38-114	Gly-Pro39+Val-peptide	P		-	-	Gly	Pro	Val	Ser	Ala	Val	Ajami et al., 2008
stromal cell-derived factor 1	P48061	22-93	Lys-Pro23+Val-peptide	P		-	-	Lys	Pro	Val	Ser	Leu	Ser	Christopherson et al., 2002	3638
Substance P	P20366	58-68	Arg-Pro59+Lys-peptide	P	-	-	-	Arg	Pro	Lys	Pro	Gln	Gln	Sedo et al., 2005
Val-Ala-NHPhNO2			Val-Ala+NHPhNO2	S		-	-	Val	Ala	NAN	-	-	-	Bjelke et al., 2006