Substrates for peptidase S01.212: plasma kallikrein

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Pharma

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	Evidence	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference	CutDB
arginyl bond			pep-Arg+pep	N		-	-	NPe	Arg	CPe	-	-	-	Colman, 2004
Boc-Gln-Arg-Arg-NHMec			Boc-Gln-Arg-Arg+NHMec	S		Boc	Gln	Arg	Arg	AMC	-	-	-	Takahashi & Ohnishi, 1998
Bz-Pro-Phe-Arg-NHPhNO2			Bz-Pro-Phe-Arg+NHPhNO2	S		Bz	Pro	Phe	Arg	NAN	-	-	-	Colman, 2004
coagulation factor XII	P00748	20-615	peptide-Arg353+Val-peptide	P		Ser	Met	Thr	Arg	Val	Val	Gly	Gly	Cool et al., 1985	17347
coagulation factor XII	P00748	20-615	peptide-Arg353+Asn-peptide	P		Ser	Leu	Thr	Arg	Asn	Gly	Pro	Leu	Cool et al., 1985
coagulation factor XII	P00748	20-615	peptide-Arg362+Leu-peptide	P		Cys	Gly	Gln	Arg	Leu	Arg	Lys	Ser	Cool et al., 1985
H-kininogen	P01044	19-644	peptide-Arg388+Ser-peptide	N		Ser	Pro	Phe	Arg	Ser	Val	Gln	Val
H-kininogen	P01044	19-644	peptide-Arg498+Thr-peptide	N		Tyr	Asp	Trp	Arg	Thr	Pro	Tyr	Leu
hepatocyte growth factor	P14210	32-728	peptide-Arg494+Val-peptide	P		Lys	Gln	Leu	Arg	Val	Val	Asn	Gly	Peek et al., 2002
hepatocyte growth factor	P14210	32-728	peptide-Arg424+His-peptide	P		Asp	Leu	His	Arg	His	Ile	Phe	Trp	Peek et al., 2002
hepatocyte growth factor-like protein	P26927	19-711	peptide-Arg483+Val-peptide	P		Ser	Lys	Leu	Arg	Val	Val	Gly	Gly	Yoshikawa et al., 1999	3630
HGFAC	Q53X47	1-655	peptide-Arg372+Val-peptide	P		Ser	Leu	Thr	Arg	Val	Gln	Leu	Ser	Shimomura et al., 1993	3629
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg+Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N		Cya	Gly	Glu	Arg	Gly	Phe	Phe	Tyr	Tang et al., 2005
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys+Ala	N		Tyr	Thr	Pro	Lys	Ala	-	-	-	Tang et al., 2005
kininogen-1	P01042	19-644	peptide-Lys362+Arg-peptide	P		Ser	Leu	Met	Lys	Arg	Pro	Pro	Gly		17349
kininogen-1	P01042	19-644	peptide-Arg371+Ser-peptide	P		Ser	Pro	Phe	Arg	Ser	Ser	Arg	Ile		17348
kininogen-1	P01042	19-644	peptide-Arg427+Arg-peptide	P		Gly	His	Thr	Arg	Arg	His	Asp	Trp	Charlesworth et al., 1999	21067
kininogen-2	P01045	19-619	peptide-Arg386+Ser-peptide	N		Ser	Pro	Phe	Arg	Ser	Val	Gln	Val		17351
kininogen-2	P01045	19-619	peptide-Arg496+Thr-peptide	N		Tyr	Asp	Trp	Arg	Thr	Pro	Tyr	Leu		17352
lysyl bond			pep-Lys+pep	N		-	-	NPe	Lys	CPe	-	-	-	Colman, 2004
matrix metallopeptidase-1 precursor	P03956	20-469	peptide-Arg54+Arg-peptide	P		Val	Glu	Lys	Arg	Arg	Asn	Ser	Gly	Suzuki et al., 1991	18341
neuropeptide Y	P01303	29-64	Tyr-Pro-Ser-Ser-Leu-Lys-Pro-Asp-Asn-Pro-Gly-Gly-Glu-Asp-Ala-Pro-Ala-Gly-Glu-Asp-Met-Ala-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Leu-Asn-Leu-Ile-Thr-Arg-Gln-Arg+Tyr-NH2	P		Thr	Arg	Gln	Arg	Tyr	NH2	-	-	Abid et al., 2009
nidogen-1	P10493	29-1245	peptide-Arg349+Ser-peptide	P		Glu	Arg	Thr	Arg	Ser	Phe	Gln	Leu	Walker et al., 1998
plasma kallikrein	P03952	20-390; 391-638	peptide-Lys140+Ala-peptide	P		Thr	Phe	His	Lys	Ala	Glu	Tyr	Arg	Colman, 2004	17357
Proenkephalin-A	P01211	233-263	Peptide-Arg256+Tyr-Peptide	N	AA	Met	Glu	Lys	Arg	Tyr	Gly	Gly	Phe	Metters et al., 1988
Proenkephalin-A	P01211	206-230	Peptide-Arg211+Arg-Peptide	N	AA	Gly	Phe	Met	Arg	Arg	Val	Gly	Arg	Metters et al., 1988
Proenkephalin-A	P01211	206-230	Peptide-Lys224+Arg-Peptide	N	AA	Asp	Tyr	Gln	Lys	Arg	Tyr	Gly	Gly	Metters et al., 1988
Proenkephalin-A	P01211	206-230	Peptide-Arg225+Tyr-Peptide	N	AA	Tyr	Gln	Lys	Arg	Tyr	Gly	Gly	Phe	Metters et al., 1988
Proenkephalin-A	P01211	104-137	Peptide-Lys109+Lys-Peptide	N	AA	Gly	Phe	Met	Lys	Lys	Met	Asp	Glu	Metters et al., 1988
Proenkephalin-A	P01211	104-137	Peptide-Lys110+Met-Peptide	N	AA	Phe	Met	Lys	Lys	Met	Asp	Glu	Leu	Metters et al., 1988
Proenkephalin-A	P01211	104-137	Peptide-Lys131+Arg-Peptide	N	AA	Val	Leu	Gly	Lys	Arg	Tyr	Gly	Gly	Metters et al., 1988
Proenkephalin-A	P01211	104-137	Peptide-Arg132+Tyr-Peptide	N	AA	Leu	Gly	Lys	Arg	Tyr	Gly	Gly	Phe	Metters et al., 1988
Proenkephalin-A	P01211	25-263	Peptide-Lys95+Lys-Peptide	N	AA	Leu	Leu	Ala	Lys	Lys	Tyr	Gly	Gly	Metters et al., 1988
Proenkephalin-A	P01211	25-263	Peptide-Lys96+Tyr-Peptide	N	AA	Leu	Ala	Lys	Lys	Tyr	Gly	Gly	Phe	Metters et al., 1988
Proenkephalin-A	P01211	25-263	Peptide-Arg103+Tyr-Peptide	N	AA	Phe	Met	Lys	Arg	Tyr	Gly	Gly	Phe	Metters et al., 1988
Proenkephalin-A	P01211	25-263	Peptide-Arg181+Tyr-Peptide	N	AA	Val	Ser	Lys	Arg	Tyr	Gly	Gly	Phe	Metters et al., 1988
Proenkephalin-A	P01211	25-263	Peptide-Arg191+Ser-Peptide	N	AA	Gly	Leu	Lys	Arg	Ser	Pro	His	Leu	Metters et al., 1988
Tos-Arg-OMe (TAME)			Tos-Arg+OMe	S		-	-	Tos	Arg	OMe	-	-	-
urokinase-type plasminogen activator	P00749	21-431	peptide-Lys158+Ile-peptide	P		Pro	Arg	Phe	Lys	Ile	Ile	Gly	Gly	Ellis, 2004	17358
Z-Phe-Arg-NHPhNO2			Z-Phe-Arg+NHPhNO2	S		-	Z	Phe	Arg	NAN	-	-	-