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Substrates for peptidase M16.002: insulysin

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate Uniprot Residue range Cleavage Site Cleavage type Evidence P4 P3 P2 P1 P1' P2' P3' P4' Reference CutDB MERNUM
amyloid beta A4 protein P05067 672-713 peptide-His684+His-peptide N Tyr Glu Val His His Gln Lys Leu Yan et al., 2006
amyloid beta A4 protein P05067 672-713 peptide-His685+Gln-peptide N Glu Val His His Gln Lys Leu Val Yan et al., 2006
amyloid beta A4 protein P05067 672-713 peptide-Gln686+Lys-peptide N Val His His Gln Lys Leu Val Phe Yan et al., 2006
amyloid beta A4 protein P05067 672-713 peptide-Lys687+Leu-peptide N His His Gln Lys Leu Val Phe Phe Yan et al., 2006
amyloid beta A4 protein P05067 672-713 peptide-Leu688+Val-peptide N His Gln Lys Leu Val Phe Phe Ala Yan et al., 2006
amyloid beta A4 protein P05067 672-713 peptide-Val689+Phe-peptide N Gln Lys Leu Val Phe Phe Ala Glu Yan et al., 2006
amyloid beta A4 protein P05067 672-713 peptide-Phe690+Phe-peptide N Lys Leu Val Phe Phe Ala Glu Asp Yan et al., 2006
amyloid beta A4 protein P05067 18-770 peptide-Lys699+Gly-peptide N Gly Ser Asn Lys Gly Ala Ile Ile Yan et al., 2006
atrial natriuretic factor P01160 124-151 Ser-Leu-Arg+Arg-peptide N - Ser Leu Arg Arg Ser Ser Cys Cornista et al., 2004
atrial natriuretic factor P01160 124-151 peptide-Cys7+Phe-peptide N Arg Ser Ser Cys Phe Gly Gly Arg Cornista et al., 2004
atrial natriuretic factor P01160 124-151 peptide-Asp13+Arg-peptide N Gly Arg Met Asp Arg Ile Gly Ala Cornista et al., 2004
atrial natriuretic factor P01160 124-151 peptide-Ser25+Phe-Arg-Tyr N Gly Cys Asn Ser Phe Arg Tyr - Cornista et al., 2004
beta-amyloid protein 42 P05067 672-713 peptide-Glu674+Phe-peptide P - Asp Ala Glu Phe Arg His Asp Yan et al., 2006
beta-amyloid protein 42 P05067 672-713 peptide-Phe675+Arg-peptide P Asp Ala Glu Phe Arg His Asp Ser Yan et al., 2006
beta-amyloid protein 42 P05067 672-713 peptide-Val683+His-peptide P NT Gly Tyr Glu Val His His Gln Lys Malito et al., 2008
beta-amyloid protein 42 P05067 672-713 peptide-Phe691+Ala-peptide P NT Leu Val Phe Phe Ala Glu Asp Val Malito et al., 2008
beta-amyloid protein 42 P05067 672-713 peptide-His684+His-peptide P MS Tyr Glu Val His His Gln Lys Leu Yan et al., 2006
beta-amyloid protein 42 P05067 672-713 peptide-His685+Gln-peptide P MS Glu Val His His Gln Lys Leu Val Yan et al., 2006
beta-amyloid protein 42 P05067 672-713 peptide-Gln686+Lys-peptide P MS Val His His Gln Lys Leu Val Phe Yan et al., 2006
beta-amyloid protein 42 P05067 672-713 peptide-Lys687+Leu-peptide P MS His His Gln Lys Leu Val Phe Phe Yan et al., 2006
beta-amyloid protein 42 P05067 672-713 peptide-Leu688+Val-peptide P MS His Gln Lys Leu Val Phe Phe Ala Yan et al., 2006
beta-amyloid protein 42 P05067 672-713 peptide-Val689+Phe-peptide P MS Gln Lys Leu Val Phe Phe Ala Glu Yan et al., 2006
beta-amyloid protein 42 P05067 672-713 peptide-Phe690+Phe-peptide P MS Lys Leu Val Phe Phe Ala Glu Asp Yan et al., 2006
beta-amyloid protein 42 P05067 672-713 peptide-Lys699+Gly-peptide P MS Gly Ser Asn Lys Gly Ala Ile Ile Yan et al., 2006
beta-endorphin P01189 237-267 peptide-Phe18+Lys-peptide N Val Thr Leu Phe Lys Asn Ala Ile Cornista et al., 2004
beta-endorphin P01189 237-267 peptide-Leu17+Phe-peptide N Leu Val Thr Leu Phe Lys Asn Ala Cornista et al., 2004
Bradykinin P01042 381-389 peptide-Pro387+Phe-Arg N MS Gly Phe Ser Pro Phe Arg - - Malito et al., 2008
brain natriuretic peptide P07634 105-131 peptide-Gly111+Arg-peptide P Gly Cys Phe Gly Arg Arg Leu Asp Müller et al., 1992 18771
brain natriuretic peptide P07634 105-131 peptide-Asp115+Arg-peptide P Arg Arg Leu Asp Arg Ile Gly Ser Müller et al., 1992 18772
brain natriuretic peptide P07634 105-131 peptide-Arg129+Arg-peptide P Asn Val Leu Arg Arg Tyr Gly Gly Müller et al., 1992 18773
dynorphin A(1-13) P01214 209-221 Tyr-Gly-Gly-Phe-Leu-Arg+Arg-Ile-Arg-Pro-Lys-Leu-Lys N Gly Phe Leu Arg Arg Ile Arg Pro Roth, 2004
insulin P01317 25-54,85-105 peptide-Ser33+His-peptide P Leu Cys Gly Ser His Leu Val Glu Malito et al., 2008
insulin P01317 25-54,85-105 peptide-His34+Leu-peptide P Cys Gly Ser His Leu Val Glu Ala Malito et al., 2008
insulin P01317 25-54,85-105 peptide-Ala38+Leu-peptide P Leu Val Glu Ala Leu Tyr Leu Val Malito et al., 2008
insulin P01317 25-54,85-105 peptide-Phe48+Phe-peptide N Glu Arg Gly Phe Phe Tyr Thr Pro Malito et al., 2008
insulin P01317 25-54,85-105 peptide-Phe49+Tyr-peptide P Arg Gly Phe Phe Tyr Thr Pro Lys Malito et al., 2008
insulin P01317 25-54,85-105 peptide-Leu97+ P Val Cys Ser Leu - - - - Malito et al., 2008
insulin P01317 25-54,85-105 peptide-Tyr98+ P Cys Ser Leu Tyr - - - - Malito et al., 2008
insulin P01308 25-54,90-110 peptide-Ser33+His-peptide P MS Leu Cys Gly Ser His Leu Val Glu Guo et al., 2009
insulin P01308 25-54,90-110 peptide-His34+Leu-peptide P MS Cys Gly Ser His Leu Val Glu Ala Guo et al., 2009
insulin P01308 25-54,90-110 peptide-Glu37+Ala-peptide P MS His Leu Val Glu Ala Leu Tyr Leu Guo et al., 2009
insulin P01308 25-54,90-110 peptide-Ala38+Leu-peptide P MS Leu Val Glu Ala Leu Tyr Leu Val Guo et al., 2009
insulin P01308 25-54,90-110 peptide-Phe48+Phe-peptide P MS Glu Arg Gly Phe Phe Tyr Thr Pro Guo et al., 2009
insulin P01308 25-54,90-110 peptide-Phe49+Tyr-peptide P MS Arg Gly Phe Phe Tyr Thr Pro Lys Guo et al., 2009
insulin P01308 25-54,90-110 peptide-Leu102+Tyr-peptide P MS Ile Cys Ser Leu Tyr Gln Leu Glu Guo et al., 2009
insulin P01308 25-54,90-110 peptide-Tyr103+Gln-peptide P MS Cys Ser Leu Tyr Gln Leu Glu Asn Guo et al., 2009
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu+Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N His Leu Val Glu Ala Leu Tyr Leu Roth, 2004
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr+Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N Glu Ala Leu Tyr Leu Val Cya Gly Roth, 2004
insulin-like growth factor II P01344 25-91 peptide-Thr40+Leu-peptide P MS Leu Val Asp Thr Leu Gln Phe Val Guo et al., 2009
insulin-like growth factor II P01344 25-91 peptide-Gln42+Phe-peptide P MS Asp Thr Leu Gln Phe Val Cys Gly Guo et al., 2009
insulin-like growth factor II P01344 25-91 peptide-Phe50+Tyr-peptide P MS Asp Arg Gly Phe Tyr Phe Ser Arg Guo et al., 2009
insulin-like growth factor II P01344 25-91 peptide-Tyr51+Phe-peptide P MS Arg Gly Phe Tyr Phe Ser Arg Pro Guo et al., 2009
insulin-like growth factor II P01344 25-91 peptide-Phe52+Ser-peptide P MS Gly Phe Tyr Phe Ser Arg Pro Ala Guo et al., 2009
insulin-like growth factor II P01344 25-91 peptide-Ser60+Arg-peptide P MS Ser Arg Val Ser Arg Arg Ser Arg Guo et al., 2009
insulin-like growth factor II P01344 25-91 peptide-Arg61+Arg-peptide P MS Arg Val Ser Arg Arg Ser Arg Gly Guo et al., 2009
insulin-like growth factor II P01344 25-91 peptide-Cys71+Phe-peptide P MS Glu Glu Cys Cys Phe Arg Ser Cys Guo et al., 2009
insulin-like growth factor II P01344 25-91 peptide-Phe72+Arg-peptide P MS Glu Cys Cys Phe Arg Ser Cys Asp Guo et al., 2009
insulin-like growth factor II P01344 25-91 peptide-Pro87+Ala-peptide P MS Cys Ala Thr Pro Ala Lys Ser Glu Guo et al., 2009
insulin-like growth factor II P01344 25-91 peptide-Ala88+Lys-Ser-Glu P MS Ala Thr Pro Ala Lys Ser Glu - Guo et al., 2009
natriuretic peptide precursor C P18104 105-126 peptide-Gly112+Leu-peptide P Gly Cys Phe Gly Leu Lys Leu Asp Müller et al., 1992 18774
natriuretic peptide precursor C P18104 105-126 peptide-Asp116+Arg-peptide P Leu Lys Leu Asp Arg Ile Gly Ser Müller et al., 1992 18775
natriuretic peptide precursor type A P01161 122-150 peptide-Arg125+Arg-peptide P Arg Ser Leu Arg Arg Ser Ser Cys Müller et al., 1992 18767
natriuretic peptide precursor type A P01161 122-150 peptide-Cys129+Phe-peptide P Arg Ser Ser Cys Phe Gly Gly Arg Müller et al., 1992 18768
natriuretic peptide precursor type A P01161 122-150 peptide-Asp135+Arg-peptide P Gly Arg Ile Asp Arg Ile Gly Ala Müller et al., 1992 18769
natriuretic peptide precursor type A P01161 122-150 peptide-Ser147+Phe-peptide P Gly Cys Asn Ser Phe Arg Tyr Arg Müller et al., 1992 18770
polyubiquitin-B P0CG47 1-76 peptide-Arg74+Gly-Gly P Ms Leu Arg Leu Arg Gly Gly - - Ralat et al., 2011
polyubiquitin-B P0CG47 1-74 peptide-Arg72+Leu-Arg P MS Leu Val Leu Arg Leu Arg - - Ralat et al., 2011
polyubiquitin-B P0CG47 77-152 peptide-Arg150+Gly-Gly P MS Leu Arg Leu Arg Gly Gly - - Ralat et al., 2011
polyubiquitin-B P0CG47 77-150 peptide-Arg148+Leu-Arg P MS Leu Val Leu Arg Leu Arg - - Ralat et al., 2011
polyubiquitin-B P0CG47 153-228 peptide-Arg226+Gly-Gly P MS Leu Arg Leu Arg Gly Gly - - Ralat et al., 2011
polyubiquitin-B P0CG47 153-226 peptide-Arg224+Leu-Arg P MS Leu Val Leu Arg Leu Arg - - Ralat et al., 2011
polyubiquitin-C P0CG48 1-76 peptide-Arg74+Gly-Gly N MS Leu Arg Leu Arg Gly Gly - - Ralat et al., 2011
polyubiquitin-C P0CG48 1-74 peptide-Arg72+Leu-Arg P MS Leu Val Leu Arg Leu Arg - - Ralat et al., 2011
polyubiquitin-C P0CG48 77-152 peptide-Arg150+Gly-Gly P MS Leu Arg Leu Arg Gly Gly - - Ralat et al., 2011
polyubiquitin-C P0CG48 77-150 peptide-Arg148+Leu-Arg P MS Leu Val Leu Arg Leu Arg - - Ralat et al., 2011
polyubiquitin-C P0CG48 153-228 peptide-Arg226+Gly-Gly P MS Leu Arg Leu Arg Gly Gly - - Ralat et al., 2011
polyubiquitin-C P0CG48 153-226 peptide-Arg224+Leu-Arg P MS Leu Val Leu Arg Leu Arg - - Ralat et al., 2011
polyubiquitin-C P0CG48 229-304 peptide-Arg302+Gly-Gly P MS Leu Arg Leu Arg Gly Gly - - Ralat et al., 2011
polyubiquitin-C P0CG48 229-302 peptide-Arg300+Leu-Arg P MS Leu Val Leu Arg Leu Arg - - Ralat et al., 2011
polyubiquitin-C P0CG48 305-380 peptide-Arg378+Gly-Gly P MS Leu Arg Leu Arg Gly Gly - - Ralat et al., 2011
polyubiquitin-C P0CG48 305-378 peptide-Arg376+Leu-Arg P MS Leu Val Leu Arg Leu Arg - - Ralat et al., 2011
polyubiquitin-C P0CG48 381-456 peptide-Arg454+Gly-Gly P MS Leu Arg Leu Arg Gly Gly - - Ralat et al., 2011
polyubiquitin-C P0CG48 381-454 peptide-Arg452+Leu-Arg P MS Leu Val Leu Arg Leu Arg - - Ralat et al., 2011
polyubiquitin-C P0CG48 457-532 peptide-Arg530+Gly-Gly P MS Leu Arg Leu Arg Gly Gly - - Ralat et al., 2011
polyubiquitin-C P0CG48 457-530 peptide-Arg528+Leu-Arg P MS Leu Val Leu Arg Leu Arg - - Ralat et al., 2011
polyubiquitin-C P0CG48 533-608 peptide-Arg606+Gly-Gly P MS Leu Arg Leu Arg Gly Gly - - Ralat et al., 2011
polyubiquitin-C P0CG48 533-606 peptide-Arg604+Leu-Arg P MS Leu Val Leu Arg Leu Arg - - Ralat et al., 2011
polyubiquitin-C P0CG48 609-684 peptide-Arg682+Gly-Gly P MS Leu Arg Leu Arg Gly Gly - - Ralat et al., 2011
polyubiquitin-C P0CG48 609-682 peptide-Arg680+Leu-Arg P MS Leu Val Leu Arg Leu Arg - - Ralat et al., 2011
protransforming growth factor alpha P01135 40-89 peptide-Ser50+His-peptide P MS Cys Pro Asp Ser His Thr Gln Phe Guo et al., 2009
protransforming growth factor alpha P01135 40-89 peptide-Gln53+Phe-peptide P MS Ser His Thr Gln Phe Cys Phe His Guo et al., 2009
protransforming growth factor alpha P01135 40-89 peptide-Phe54+Cys-peptide P MS His Thr Gln Phe Cys Phe His Gly Guo et al., 2009
protransforming growth factor alpha P01135 40-89 peptide-Phe56+His-peptide P MS Gln Phe Cys Phe His Gly Thr Cys Guo et al., 2009
protransforming growth factor alpha P01135 40-89 peptide-Thr59+Cys-peptide P MS Phe His Gly Thr Cys Arg Phe Leu Guo et al., 2009
protransforming growth factor alpha P01135 40-89 peptide-Cys60+Arg-peptide P MS His Gly Thr Cys Arg Phe Leu Val Guo et al., 2009
protransforming growth factor alpha P01135 40-89 peptide-Cys73+His-peptide P MS Ala Cys Val Cys His Ser Gly Tyr Guo et al., 2009
protransforming growth factor alpha P01135 40-89 peptide-His74+Ser-peptide P MS Cys Val Cys His Ser Gly Tyr Val Guo et al., 2009
protransforming growth factor alpha P01135 40-89 peptide-Gly79+Ala-peptide P MS Gly Tyr Val Gly Ala Arg Cys Glu Guo et al., 2009
somatostatin-14 P61278 103-116 peptide-Phe108+Phe-peptide P Cys Lys Asn Phe Phe Trp Lys Thr Ciacco et al., 2008
vasoactive intestinal peptide His-Ser-Asp-Ala-Val-Phe-Thr-Asp-Asn-Tyr-Thr-Arg-Leu+Arg-Lys-Gln-Met-Ala-Val-Lys-Lys-Tyr-Leu-Asn-Ser-Ile-Leu-Asn-NH2 N Tyr Thr Arg Leu Arg Lys Gln Met Cornista et al., 2004
[Lys]-bradykinin (kallidin) P01042 380-389 peptide-Pro387+Phe-Arg N MS Gly Phe Ser Pro Phe Arg - - Malito et al., 2008

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