Substrates for peptidase M14.004: carboxypeptidase N

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Pharma

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	Evidence	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference	CutDB
bradykinin	P01042	381-389	Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe+Arg	P		Phe	Ser	Pro	Phe	Arg	-	-	-		17031
C3a anaphylatoxin	P01024	672-748	peptide-Ala76+Arg	P		Leu	Gly	Leu	Ala	Arg	-	-	-		17032
C4A anaphylatoxin	P0C0L4	680-756	peptide-Gln76+Arg	P		Ala	Gly	Leu	Gln	Arg	-	-	-		17033
C5a anaphylatoxin	P01031	678-751	peptide-Gly73+Arg	P		Met	Gln	Leu	Gly	Arg	-	-	-		17034
Dns-Ala-Arg			Dns-Ala+Arg	S		-	-	Dns	Ala	Arg	-	-	-	Skidgel & Erdos, 2004
Fibrinogen beta chain	P02675	169-178	peptide-Glu177+Lys	N	NT	Ser	Glu	Leu	Glu	Lys	-	-	-	Myles et al., 2003
Fibrinogen gamma chain	P02679	79-88	peptide-Ile87+Lys	N	NT	Lys	Gln	Leu	Ile	Lys	-	-	-	Myles et al., 2003
Fibrinogen gamma chain	P02679	102-111	peptide-Leu110+Lys	N	NT	Ala	Ala	Thr	Leu	Lys	-	-	-	Myles et al., 2003
furylacryloyl-Ala-Lys (FAAK)			furylacryloyl-Ala+Lys	S		-	-	FA	Ala	Lys	-	-	-	Skidgel & Erdos, 2004
hemoglobin subunit alpha	P69905	2-142	peptide-Tyr141+Arg	P		Thr	Ser	Lys	Tyr	Arg	-	-	-	Michel et al., 1996
N-(4-furylacryloyl)-Ala-Lys-OH			N-(4-furylacryloyl)-Ala+Lys-OH	S		-	-	FA	Ala	Lys	-	-	-	Pallares et al., 2005
Osteopontin	P10451	160-168	peptide-Leu167+Arg	N	NT	Val	Tyr	Gly	Leu	Arg	-	-	-	Myles et al., 2003
protein S100-A10	P60903	2-97	peptide-Lys96+Lys	P		Gln	Lys	Gly	Lys	Lys	-	-	-	Fogg et al., 2002
protein S100-A10	P60903	2-97	peptide-Gly95+Lys	P		Lys	Gln	Lys	Gly	Lys	-	-	-	Fogg et al., 2002
stromal cell-derived factor 1	P48061	22-93	peptide-Asn88+Lys	P		Lys	Ala	Leu	Asn	Lys	-	-	-	Davis et al., 2005