Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.
| Substrate | Uniprot | Residue range | Cleavage Site | Cleavage type | Evidence | P4 | P3 | P2 | P1 | P1' | P2' | P3' | P4' | Reference | CutDB | MERNUM |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Abz-Leu-Phe-Lys(Dnp) | Abz-Leu+Phe-Lys(Dnp) | S | - | - | Abz | Leu | Phe | Lyd | - | - | Bersanetti et al., 2004 | |||||
| Abz-Ser-Asp-Lys(Dnp)-Pro | Abz-Ser-Asp+Lys(Dnp)-Pro | S | - | Abz | Ser | Asp | Lyd | Pro | - | - | Araujo et al., 2000 | |||||
| Ac-Ser-Asp-Lys-Pro | Ac-Ser-Asp+Lys-Pro | S | - | Ac | Ser | Asp | Lys | Pro | - | - | Corvol et al., 2004 | |||||
| Angiotensin 1-7 | P01019 | 34-40 | Asp-Arg-Val-Tyr-Ile+His-Pro | P | NT | Arg | Val | Tyr | Ile | His | Pro | - | - | Deddish et al., 1998 | ||
| angiotensin-1 | P01019 | 34-43 | Asp-Arg-Val-Tyr-Ile-His-Pro-Phe+His-Leu | P | Ile | His | Pro | Phe | His | Leu | - | - | Corvol et al., 2004 | 17821 | ||
| beta-neoendorphin | P01213 | 175-183 | Tyr-Gly-Gly-Phe-Leu-Arg-Lys+Tyr-Pro | N | Phe | Leu | Arg | Lys | Tyr | Pro | - | - | Corvol et al., 2004 | 17822 | ||
| bradykinin | P01042 | 381-389 | Arg-Pro-Pro-Gly-Phe-Ser-Pro+Phe-Arg | P | Gly | Phe | Ser | Pro | Phe | Arg | - | - | Corvol et al., 2004 | 17823 | ||
| Bz-Gly-His-Leu | Bz-Gly+His-Leu | S | - | - | Bz | Gly | His | Leu | - | - | Corvol et al., 2004 | |||||
| cholecystokinin-8 | P06307 | 96-103 | Asp-Tyr-Met-Gly-Trp-Met+Asp-Phe-NH2 | N | Met | Gly | Trp | Met | Asp | Phe | NH2 | - | Corvol et al., 2004 | |||
| FA-Phe-Gly-Gly | furylacryloyl-Phe+Gly-Gly | S | - | - | FA | Phe | Gly | Gly | - | - | Corvol et al., 2004 | |||||
| gonadoliberin-1 | P01148 | 24-33 | Glp-His-Trp-Ser-Tyr-Gly-Leu+Arg-Pro-Gly-NH2 | N | Ser | Tyr | Gly | Leu | Arg | Pro | Gly | NH2 | Corvol et al., 2004 | |||
| gonadoliberin-1 | P01148 | 24-33 | Glp-His-Trp+Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2 | N | - | Glp | His | Trp | Ser | Tyr | Gly | Leu | Corvol et al., 2004 | |||
| insulin B chain (oxidized) | P01317 | 25-54 | Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro+Lys-Ala | N | Phe | Tyr | Thr | Pro | Lys | Ala | - | - | Corvol et al., 2004 | |||
| K(Dnp)PPGFSPK(Abz)NH2 | Lys(Dnp)-Pro-Pro-Gly-Phe+Ser-Pro-Lys(Abz)NH2 | S | Pro | Pro | Gly | Phe | Ser | Pro | Lya | NH2 | Molinaro et al., 2005 | |||||
| Met-enkephalin-Arg-Phe | P01210 | 261-267 | Tyr-Gly-Gly-Phe-Met+Arg-Phe | P | Gly | Gly | Phe | Met | Arg | Phe | - | - | Trieu et al., 2022 | |||
| neurotensin | P30990 | 151-163 | Glp-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr+Ile-Leu | N | Arg | Arg | Pro | Tyr | Ile | Leu | - | - | Skidgel & Erdös, 2004 | 17828 | ||
| pro-opiomelanocortin | P01189 | 138-150 | Ser-Tyr+Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Val | P | CS | - | - | Ser | Tyr | Ser | Met | Glu | His | Scholzen et al., 2007 | ||
| pro-opiomelanocortin | P01189 | 140-150 | Ser-Met+Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Val | P | CS | - | - | Ser | Met | Glu | His | Phe | Arg | Scholzen et al., 2007 | ||
| pro-opiomelanocortin | P01189 | 142-150 | Glu-His+Phe-Arg-Trp-Gly-Lys-Pro-Val | P | CS | - | - | Glu | His | Phe | Arg | Trp | Gly | Scholzen et al., 2007 | ||
| pro-opiomelanocortin | P01189 | 144-150 | Phe-Arg+Trp-Gly-Lys-Pro-Val | P | CS | - | - | Phe | Arg | Trp | Gly | Lys | Pro | Scholzen et al., 2007 | ||
| pro-opiomelanocortin | P01189 | 146-150 | Trp-Gly+Lys-Pro-Val | P | MS | - | - | Trp | Gly | Lys | Pro | Val | - | Scholzen et al., 2007 | ||
| retinoic acid receptor responder protein 2 precursor | Q99969 | 145-154 | peptide-Gly152+Gln-peptide | P | Tyr | Phe | Pro | Gly | Gln | Phe | Ala | Phe | John et al., 2007 | |||
| substance P | P20366 | 58-68 | Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe+Gly-Leu-Met-NH2 | N | Gln | Gln | Phe | Phe | Gly | Leu | Met | NH2 | Corvol et al., 2004 | |||
| Z-Phe-His-Leu | Z-Phe+His-Leu | N | - | - | Z | Phe | His | Leu | - | - | Danilov et al., 1994 | |||||
| [desArg9]-bradykinin | P01042 | 381-388 | Arg-Pro-Pro-Gly-Phe-Ser+Pro-Phe | N | Pro | Gly | Phe | Ser | Pro | Phe | - | - | Corvol et al., 2004 | |||
| [Met5]-enkephalin | P01189 | 237-241 | Tyr-Gly-Gly+Phe-Met-NH2 | N | - | Tyr | Gly | Gly | Phe | Met | NH2 | - | Corvol et al., 2004 | |||
| [Met5]-enkephalin-Arg-Gly-Leu | Tyr-Gly-Gly-Phe-Met-Arg+Gly-Leu | N | Gly | Phe | Met | Arg | Gly | Leu | - | - | Corvol et al., 2004 |
