Substrates for peptidase M13.003: endothelin-converting enzyme 2

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Pharma

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	Evidence	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference	CutDB
angiotensin-1	P01019	34-43	peptide-Pro40+Phe-peptide	P	MS	Tyr	Ile	His	Pro	Phe	His	Leu	-	Mzhavia et al., 2003	24178
big endothelin 1	P05305	53-90	peptide-Trp21+Val22-peptide	P		Asp	Ile	Ile	Trp	Val	Asn	Thr	Pro	Mzhavia et al., 2003	24173
bradykinin	P01042	381-389	Arg-Pro-Pro-Gly-Phe-Ser-Pro+Phe-Arg	P	MS	Gly	Phe	Ser	Pro	Phe	Arg	-	-	Mzhavia et al., 2003	24175
dynorphin B	P01213	226-238	peptide-Gln233+Phe-peptide	P	MS	Leu	Arg	Arg	Gln	Phe	Lys	Val	Val	Mzhavia et al., 2003	24186
dynorphin B	P01213	226-238	Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Gln-Phe-Lys+Val-Val-Thr	P	MS	Arg	Gln	Phe	Lys	Val	Val	Thr	Arg	Mzhavia et al., 2003
endothelin-1 (1-38)			peptide-Trp21+Ile22-peptide	N		Asp	Ile	Ile	Trp	Ile	Asn	Thr	Pro	Ahn, 2004
little PEN-LEN	Q9QXU9	221-254	peptide-Ala238+Leu-peptide	P	MS	Val	Leu	Gly	Ala	Leu	Leu	Arg	Val	Mzhavia et al., 2003	24187
little PEN-LEN	Q9QXU9	221-254	peptide-Leu240+Arg-peptide	P	MS	Gly	Ala	Leu	Leu	Arg	Val	Lys	Arg	Mzhavia et al., 2003	24188
little PEN-LEN	Q9QXU9	221-254	peptide-Arg241+Val-peptide	P	MS	Ala	Leu	Leu	Arg	Val	Lys	Arg	Val	Mzhavia et al., 2003	24189
neurotensin	P30990	24-148	peptide-Pro160+Tyr-peptide	P	MS	Pro	Arg	Arg	Pro	Tyr	Ile	Leu	-	Mzhavia et al., 2003	24176
neurotensin	P30990	24-148	peptide-Tyr161+Ile-peptide	P	MS	Arg	Arg	Pro	Tyr	Ile	Leu	-	-	Mzhavia et al., 2003	24177
peptide E	P01211	206-230	peptide-Asp221+Tyr-peptide	P	MS	Trp	Trp	Met	Asp	Tyr	Gln	Lys	Arg	Mzhavia et al., 2003	24183
peptide E	P01211	206-230	peptide-Gly228+Phe-peptide	P	MS	Arg	Tyr	Gly	Gly	Phe	Leu	-	-	Mzhavia et al., 2003	24184
Proenkephalin-A	P01211	206-227	peptide-Glu217+Trp-peptide	P	MS	Gly	Arg	Pro	Glu	Trp	Trp	Met	Asp	Mzhavia et al., 2003	24185
Proenkephalin-A	P01211	206-223	Tyr-Gly-Gly-Phe-Met-Arg-Arg-Val-Gly-Arg-Pro-Glu+Trp-Trp-Met-Asp-Tyr-Gln	P	MS	Gly	Arg	Pro	Glu	Trp	Trp	Met	Asp	Mzhavia et al., 2003
Proenkephalin-A	P01211	206-223	Tyr-Gly-Gly-Phe-Met-Arg-Arg-Val-Gly-Arg-Pro-Glu-Trp-Trp-Met-Asp+Tyr-Gln	P	MS	Trp	Trp	Met	Asp	Tyr	Gln	-	-	Mzhavia et al., 2003
substance P	P20366	58-68	peptide-Gly66+Leu-peptide	P	MS	Gln	Phe	Phe	Gly	Leu	Met	NH2	-	Mzhavia et al., 2003	24180