Substrates for peptidase C25.001: gingipain RgpA

Summary Alignment Sequences Sequence features Distribution Literature Substrates

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	Evidence	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference	CutDB
apolipoprotein B-100	P04114	28-4563	peptide-Arg158+Gly-peptide	N		Asn	Ile	Lys	Arg	Gly	Ile	Ile	Ser	Bengtsson et al., 2008	20574
apolipoprotein B-100	P04114	28-4563	peptide-Arg207+Asp-peptide	N		Ser	Thr	Glu	Arg	Asp	Leu	Gly	Gln	Bengtsson et al., 2008	20575
Bz-Arg-NHPhNO2 (BAPNA)			Bz-Arg+NHPhNO2	S		-	-	Bz	Arg	NAN	-	-	-	Imamura et al., 2004
coagulation factor IX	P00740	47-461	peptide-Arg191+Ala-peptide	N		Lys	Leu	Thr	Arg	Ala	Glu	Thr	Val	Imamura et al., 2001	20589
coagulation factor IX	P00740	47-461	peptide-Arg226+Val-peptide	N		Asp	Phe	Thr	Arg	Val	Val	Gly	Gly	Imamura et al., 2001	20590
coagulation factor X light chain	P00742	41-488	peptide-Arg469+Gly-peptide	N	CS	Met	Lys	Thr	Arg	Gly	Leu	Pro	Lys	Imamura et al., 1997	20579
coagulation factor X light chain	P00742	41-488	peptide-Arg227+Gly-peptide	N	CS	Gln	Pro	Glu	Arg	Gly	Asp	Asn	Asn	Imamura et al., 1997	20580
coagulation factor X light chain	P00742	41-488	peptide-Arg234+Ile-peptide	N	CS	Asn	Leu	Thr	Arg	Ile	Val	Gly	Gly	Imamura et al., 1997	20581
consensus arginyl bond			peptide-Arg+peptide	N		-	-	NPe	Arg	CPe	-	-	-	Imamura et al., 2004
cystatin C	P01034	27-146	peptide-Arg34+Leu-peptide	N		Lys	Pro	Pro	Arg	Leu	Val	Gly	Gly	Abrahamson et al., 1998	20563
elafin	P19957	61-117	peptide-Arg82+Cys-peptide	N	MS	Ile	Leu	Ile	Arg	Cys	Ala	Met	Leu	Kantyka et al., 2009
fimbrilin type 1 long precursor isoform	P0C940	1-347	peptide-Arg46+Ala-peptide	P		Asn	Ser	Asn	Arg	Ala	Phe	Gly	Val	Imamura et al., 2004	16282
gingipain R precursor	P28784	25-991	peptide-Arg203+Tyr-peptide	P		Glu	Pro	Gly	Arg	Tyr	Thr	Pro	Val	Imamura et al., 2004	16287
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg+Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N		Cya	Gly	Glu	Arg	Gly	Phe	Phe	Tyr	Imamura et al., 2004
interleukin-6	P05231	30-212	peptide-Arg44+Gln-peptide	N		Ala	Pro	His	Arg	Gln	Pro	Leu	Thr	Banbula et al., 1999	18862
interleukin-6	P05231	30-212	peptide-Arg207+Ala-peptide	N		Ser	Ser	Leu	Arg	Ala	Leu	Arg	Gln	Banbula et al., 1999	18863
interleukin-8	P10145	23-99	peptide-Arg27+Ser-peptide	N		Val	Leu	Pro	Arg	Ser	Ala	Lys	Glu	Dias et al., 2007	20570
interleukin-8	P10145	23-99	peptide-Arg33+Cys-peptide	N		Lys	Glu	Leu	Arg	Cys	Gln	Cys	Ile	Dias et al., 2007	20573
kininogen-1	P01042	19-644	peptide-Arg371+Ser-peptide	N		Ser	Pro	Phe	Arg	Ser	Ser	Arg	Ile	Imamura et al., 2004
kininogen-1	P01042	19-644	peptide-Lys362+Arg-peptide	N		Ser	Leu	Met	Lys	Arg	Pro	Pro	Gly	Imamura et al., 2004
mellitin	P01501	44-69	Gly-Ile-Gly-Ala-Val-Leu-Lys-Val-Leu-Thr-Thr-Gly-Leu-Pro-Ala-Leu-Ile-Ser-Trp-Ile-Lys-Arg+Lys-Arg-Gln-Gln-NH2	N		Trp	Ile	Lys	Arg	Lys	Arg	Gln	Gln	Imamura et al., 2004
mellitin	P01501	44-69	Gly-Ile-Gly-Ala-Val-Leu-Lys-Val-Leu-Thr-Thr-Gly-Leu-Pro-Ala-Leu-Ile-Ser-Trp-Ile-Lys-Arg-Lys-Arg+Gln-Gln-NH2	N		Lys	Arg	Lys	Arg	Gln	Gln	NH2	-	Imamura et al., 2004
N-acetyl-S-farnesyl-Cys-Val-Ile-Met			N-acetyl-S-farnesyl-Cys+Val-Ile-Met	N		-	-	Ac	Cyf	Val	Ile	Met	-	Imamura et al., 2004
Pg-II fimbriae b precursor	Q8KHA3	1-561	peptide-Arg49+Ala-peptide	P		Gly	Ser	Ala	Arg	Ala	Gly	Asp	Gly	Imamura et al., 2004	16286
tumor necrosis factor	P01375	77-233	peptide-Arg78+Ser-peptide	N		-	-	Val	Arg	Ser	Ser	Ser	Arg	Calkins et al., 1998	20521
tumor necrosis factor	P01375	77-233	peptide-Arg82+Thr-peptide	N		Ser	Ser	Ser	Arg	Thr	Pro	Ser	Asp	Calkins et al., 1998	20531