Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 1 |
unknown peptidase |
1-466 |
P |
|
NT |
<%Agarwal et al., 2012[]%> |
| 36 |
granzyme A |
1-466 |
N |
|
|
Van Damme et al., 2010 |
| 38 |
cathepsin G |
36-50 |
N |
|
MS |
Schilling & Overall, 2008 |
| 40 |
HtrA2 peptidase |
2-466 |
P |
|
|
Vande Walle et al., 2007 |
| 41 |
cathepsin S |
37-50 |
N |
|
MS |
Biniossek et al., 2011 |
| 50 |
trypsin 1 |
2-466 |
N |
|
MS |
Schilling & Overall, 2008 |
| 69 |
matrix metallopeptidase-2 |
1-95 |
N |
|
MS |
Schilling & Overall, 2008 |
| 85 |
caspase-3 |
2-466 |
P |
|
|
Byun et al., 2001 |
| 85 |
caspase-7 |
2-466 |
P |
|
|
Byun et al., 2001 |
| 86 |
cathepsin G |
78-97 |
N |
|
MS |
Schilling & Overall, 2008 |
| 90 |
granzyme B (Homo sapiens-type) |
2-466 |
N |
|
MS |
Van Damme et al., 2010 |
| 95 |
glutamyl endopeptidase I |
2-466 |
N |
|
MS |
Schilling & Overall, 2008 |
| 97 |
trypsin 1 |
2-466 |
N |
|
MS |
Schilling & Overall, 2008 |
| 129 |
meprin alpha subunit |
126-140 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 131 |
HIV-1 retropepsin |
129-143 |
N |
|
MS |
Schilling & Overall, 2008 |
| 132 |
HIV-1 retropepsin |
129-143 |
N |
|
MS |
Schilling & Overall, 2008 |
| 132 |
meprin beta subunit |
129-143 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 132 |
elastase-2 |
129-143 |
N |
|
MS |
Schilling & Overall, 2008 |
| 132 |
cathepsin G |
129-143 |
N |
|
MS |
Schilling & Overall, 2008 |
| 133 |
meprin alpha subunit |
130-143 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 134 |
matrix metallopeptidase-2 |
129-143 |
N |
|
MS |
Schilling & Overall, 2008 |
| 143 |
trypsin 1 |
2-466 |
N |
|
MS |
Schilling & Overall, 2008 |
| 174 |
glutamyl endopeptidase I |
170-184 |
N |
|
MS |
Schilling & Overall, 2008 |
| 184 |
trypsin 1 |
2-466 |
N |
|
MS |
Schilling & Overall, 2008 |
| 193 |
HIV-1 retropepsin |
188-207 |
N |
|
MS |
Schilling & Overall, 2008 |
| 196 |
trypsin 1 |
2-466 |
N |
|
MS |
Schilling & Overall, 2008 |
| 205 |
matrix metallopeptidase-2 |
196-217 |
N |
|
MS |
Schilling & Overall, 2008 |
| 207 |
trypsin 1 |
2-466 |
N |
|
MS |
Schilling & Overall, 2008 |
| 217 |
trypsin 1 |
2-466 |
N |
|
MS |
Schilling & Overall, 2008 |
| 221 |
glutamyl endopeptidase I |
2-466 |
N |
|
MS |
Schilling & Overall, 2008 |
| 225 |
meprin alpha subunit |
222-236 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 226 |
meprin alpha subunit |
223-235 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 229 |
meprin alpha subunit |
226-236 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 246 |
cathepsin S |
237-255 |
N |
|
MS |
Biniossek et al., 2011 |
| 257 |
meprin beta subunit |
254-270 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 257 |
granzyme B (Homo sapiens-type) |
2-466 |
N |
|
MS |
Van Damme et al., 2010 |
| 258 |
meprin beta subunit |
255-270 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 259 |
caspase-1 |
2-466 |
N |
|
MS |
Agard et al., 2010 |
| 259 |
caspase-6 |
2-466 |
P |
|
|
Byun et al., 2001 |
| 259 |
caspase-8 |
2-466 |
P |
|
|
Morishima, 1999 |
| 259 |
caspase-9 |
2-466 |
P |
|
|
Nakanishi et al., 2001 |
| 259 |
granzyme B (Homo sapiens-type) |
2-466 |
P |
|
MS |
Van Damme et al., 2009 |
| 293 |
peptidyl-Lys metallopeptidase |
1-466 |
N |
|
MS |
|
| 312 |
peptidyl-Lys metallopeptidase |
1-466 |
N |
|
MS |
|
| 321 |
trypsin 1 |
2-466 |
N |
|
MS |
Schilling & Overall, 2008 |
| 324 |
astacin |
321-334 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 325 |
matrix metallopeptidase-2 |
321-334 |
N |
|
MS |
Schilling & Overall, 2008 |
| 329 |
astacin |
326-342 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 329 |
LAST_MAM peptidase (Limulus-type) |
326-342 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 330 |
elastase-2 |
321-342 |
N |
|
MS |
Schilling & Overall, 2008 |
| 331 |
caspase-3 |
321-342 |
N |
|
MS |
Schilling & Overall, 2008 |
| 331 |
granzyme B (Homo sapiens-type) |
2-466 |
N |
|
MS |
Van Damme et al., 2010 |
| 334 |
trypsin 1 |
2-466 |
N |
|
MS |
Schilling & Overall, 2008 |
| 342 |
trypsin 1 |
2-466 |
N |
|
MS |
Schilling & Overall, 2008 |
| 351 |
HIV-1 retropepsin |
345-364 |
N |
|
MS |
Schilling & Overall, 2008 |
| 357 |
matrix metallopeptidase-2 |
354-368 |
N |
|
MS |
Schilling & Overall, 2008 |
| 364 |
matrix metallopeptidase-2 |
358-380 |
N |
|
MS |
Schilling & Overall, 2008 |
| 364 |
trypsin 1 |
2-466 |
N |
|
MS |
Schilling & Overall, 2008 |
| 367 |
LAST_MAM peptidase (Limulus-type) |
364-378 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 368 |
glutamyl endopeptidase I |
2-466 |
N |
|
MS |
Schilling & Overall, 2008 |
| 380 |
chymotrypsin A (cattle-type) |
2-466 |
N |
|
MS |
Schilling & Overall, 2008 |
| 381 |
trypsin 1 |
2-466 |
N |
|
MS |
Schilling & Overall, 2008 |
| 390 |
trypsin 1 |
2-466 |
N |
|
MS |
Schilling & Overall, 2008 |
| 410 |
trypsin 1 |
2-466 |
N |
|
MS |
Schilling & Overall, 2008 |
| 416 |
matrix metallopeptidase-2 |
408-425 |
N |
|
MS |
Schilling & Overall, 2008 |
| 424 |
trypsin 1 |
2-466 |
N |
|
MS |
Schilling & Overall, 2008 |
| 425 |
glutamyl endopeptidase I |
2-466 |
N |
|
MS |
Schilling & Overall, 2008 |
| 428 |
meprin beta subunit |
425-440 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 428 |
LAST_MAM peptidase (Limulus-type) |
425-440 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 429 |
cathepsin K |
424-439 |
N |
|
MS |
Schilling & Overall, 2008 |
| 429 |
caspase-3 |
424-439 |
N |
|
MS |
Schilling & Overall, 2008 |
| 429 |
astacin |
426-439 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 429 |
glutamyl endopeptidase I |
424-439 |
N |
|
MS |
Schilling & Overall, 2008 |
| 430 |
meprin beta subunit |
427-440 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 439 |
trypsin 1 |
2-466 |
N |
|
MS |
Schilling & Overall, 2008 |
