| Metal MACiE Id | EC number | Enzyme name | Catalytic metals |
| M0256 | 1.1.1.1 | alcohol dehydrogenase | zinc,zinc |
| M0021 | 1.1.1.38 | malate dehydrogenase (oxaloacetate-decarboxylating) | manganese |
| M0007 | 1.1.1.42 | isocitrate dehydrogenase (NADP+) | magnesium |
| M0312 | 1.1.1.6 | glycerol dehydrogenase | zinc,potassium |
| M0102 | 1.1.2.3 | L-lactate dehydrogenase (cytochrome) | iron |
| M0322 | 1.1.3.9 | galactose oxidiase | copper |
| M0104 | 1.1.5.2 | quinoprotein glucose dehydrogenase | calcium |
| M0099 | 1.1.99.8 | quinoprotein alcohol dehydrogenase | calcium |
| M0208 | 1.10.2.2 | ubiquinol-cytochrome-c reductase | iron,iron,iron,iron |
| M0125 | 1.10.3.1 | catechol oxidase | copper,copper |
| M0278 | 1.10.99.1 | plastoquinol-plastocyanin reductase | copper |
| M0250 | 1.11.1.10 | chloride peroxidase | iron |
| M0014 | 1.11.1.10 | chloride peroxidase | vanadium |
| M0239 | 1.11.1.7 | peroxidase | calcium,calcium,iron |
| M0126 | 1.12.2.1 | cytochrome-c3 hydrogenase | iron,iron,iron,nickel,iron |
| M0127 | 1.12.7.2 | ferredoxin hydrogenase | iron,iron,iron,iron,iron |
| M0034 | 1.13.11.2 | catechol 2,3-dioxygenase | iron |
| M0128 | 1.13.12.7 | photinus-luciferin 4-monooxygenase | magnesium |
| M0129 | 1.14.11.17 | taurine dioxygenase | iron |
| M0130 | 1.14.12.12 | naphthalene 1,2-dioxygenase | iron,iron,iron,iron |
| M0133 | 1.14.15.1 | camphor 5-monooxygenase | iron |
| M0134 | 1.14.16.2 | tyrosine 3-monooxygenase | iron |
| M0135 | 1.14.17.3 | peptidylglycine monooxygenase | copper,copper |
| M0136 | 1.14.19.2 | acyl-[acyl-carrier-protein] desaturase | iron,iron |
| M0137 | 1.14.20.1 | deacetoxycephalosporin-C synthase | iron |
| M0037 | 1.14.99.1 | prostaglandin-endoperoxide synthase | iron |
| M0138 | 1.15.1.1 | superoxide dismutase | copper |
| M0277 | 1.16.1.1 | mercury(II) reductase | mercury |
| M0139 | 1.17.4.2 | ribonucleoside-triphosphate reductase | cobalt |
| M0141 | 1.17.99.1 | 4-cresol dehydrogenase (hydroxylating) | iron |
| M0212 | 1.18.6.1 | nitrogenase | magnesium,iron,molybdenum,iron,iron |
| M0275 | 1.2.2.2 | pyruvate dehydrogenase (cytochrome) | magnesium |
| M0105 | 1.2.3.1 | aldehyde oxidase | iron,iron,molybdenum |
| M0274 | 1.2.3.3 | pyruvate oxidase | magnesium |
| M0106 | 1.2.4.1 | pyruvate dehydrogenase (acetyl-transferring) | magnesium |
| M0280 | 1.2.4.4 | 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) | magnesium,potassium,potassium |
| M0119 | 1.2.7.1 | pyruvate:ferredoxin oxidoreducatse | iron,iron,iron,magnesium |
| M0107 | 1.2.99.2 | carbon-monoxide dehydrogenase (acceptor) | iron,iron,copper,molybdenum |
| M0143 | 1.20.4.1 | arsenate reductase | potassium |
| M0144 | 1.20.98.1 | arsenite oxidase | molybdenum,iron,iron |
| M0145 | 1.21.3.1 | isopenicillin-N synthase | iron |
| M0108 | 1.3.1.34 | 2,4-dienoyl-CoA reductase (NADPH) | iron |
| M0294 | 1.3.5.1 | succinate dehydrogenase (ubiquinone) | iron,iron,iron |
| M0020 | 1.3.99.1 | succinate dehydrogenase | iron,iron,iron,iron |
| M0304 | 1.4.1.13 | glutamate synthase (NADPH) | iron,iron,iron |
| M0111 | 1.4.7.1 | glutamate synthase (ferredoxin) | iron |
| M0281 | 1.5.5.1 | electron-transferring-flavoprotein dehydrogenase | iron |
| M0114 | 1.5.8.2 | trimethylamine dehydrogenase | iron |
| M0004 | 1.7.2.1 | nitrite reductase (NO-forming) | copper,copper |
| M0323 | 1.7.7.1 | nitrate reductase | iron,iron |
| M0276 | 1.7.99.4 | nitrate reductase | iron,molybdenum,iron,iron |
| M0121 | 1.8.3.1 | sulfite oxidase | molybdenum,iron |
| M0123 | 1.8.99.2 | adenylyl-sulfate reductase | iron,iron |
| M0124 | 1.9.3.1 | cytochrome-c oxidase | copper,iron,iron,copper,copper |
| M0146 | 1.97.1.2 | pyrogallol hydroxytransferase | molybdenum |
| M0268 | 2.1.1.13 | methionine synthase | cobalt |
| M0295 | 2.1.1.63 | methylated-DNA--[protein]-cysteine S-methyltransferase | zinc |
| M0219 | 2.2.1.1 | transketolase | calcium |
| M0289 | 2.2.1.6 | acetolactate synthase | magnesium |
| M0053 | 2.3.3.9 | malate synthase | magnesium |
| M0214 | 2.4.2.14 | amidophosphoribosyltransferase | magnesium,iron |
| M0008 | 2.4.2.19 | nicotinate-nucleotide diphosphorylase (carboxylating) | manganese,manganese |
| M0048 | 2.4.2.8 | hypoxanthine phosphoribosyltransferase | magnesium,magnesium |
| M0253 | 2.5.1.10 | geranyltranstransferase | magnesium,magnesium,magnesium |
| M0264 | 2.5.1.21 | squalene synthase | magnesium,magnesium,magnesium |
| M0009 | 2.5.1.6 | methionine adenosyltransferase | magnesium,magnesium |
| M0209 | 2.7.1.20 | adenosine kinase | magnesium |
| M0326 | 2.7.1.40 | pyruvate kinase | potassium,manganese,none |
| M0246 | 2.7.10.1 | receptor protein-tyrosine kinase | magnesium,magnesium |
| M0035 | 2.7.11.19 | phosphorylase kinase | magnesium,none |
| M0282 | 2.7.12.2 | mitogen-activated protein kinase kinase | magnesium |
| M0040 | 2.7.2.3 | phosphoglycerate kinase | magnesium |
| M0327 | 2.7.3.- | protein histidine kinase (CheA) | magnesium |
| M0086 | 2.7.3.3 | arginine kinase | magnesium |
| M0290 | 2.7.4.3 | adenylate kinase | magnesium |
| M0150 | 2.7.4.6 | nucleoside-diphosphate kinase | magnesium |
| M0151 | 2.7.6.3 | 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase | magnesium,magnesium |
| M0088 | 2.7.7.12 | UDP-glucose-hexose-1-phosphate uridylyltransferase | zinc,iron |
| M0299 | 2.7.7.3 | pantetheine-phosphate adenylyltransferase | magnesium |
| M0296 | 2.7.7.39 | glycerol-3-phosphate cytidylyltransferase | magnesium |
| M0152 | 2.7.8.7 | holo-[acyl-carrier-protein] synthase | magnesium |
| M0207 | 2.7.9.1 | pyruvate, phosphate dikinase | magnesium,magnesium,magnesium |
| M0156 | 2.8.4.1 | coenzyme-B sulfoethylthiotransferase | nickel |
| M0083 | 3.1.1.4 | phospholipase A2 | calcium |
| M0160 | 3.1.11.2 | exodeoxyribonuclease III | magnesium |
| M0321 | 3.1.13.5 | ribonuclease D | zinc,zinc |
| M0157 | 3.1.2.6 | hydroxyacylglutathione hydrolase | zinc,zinc |
| M0233 | 3.1.21.- | DNA endonuclease I-CreI | magnesium,magnesium,magnesium |
| M0041 | 3.1.21.1 | deoxyribonuclease I | magnesium,magnesium |
| M0011 | 3.1.21.2 | deoxyribonuclease IV (phage-T4-induced) | zinc,zinc,zinc |
| M0161 | 3.1.22.4 | crossover junction endodeoxyribonuclease | calcium |
| M0163 | 3.1.26.4 | calf thymus ribonuclease H | magnesium |
| M0044 | 3.1.3.1 | alkaline phosphatase | zinc,zinc,magnesium |
| M0043 | 3.1.3.2 | acid phosphatase | iron,zinc |
| M0042 | 3.1.30.2 | nuclease | magnesium |
| M0165 | 3.1.31.1 | micrococcal nuclease | calcium |
| M0028 | 3.1.4.11 | phosphoinositide phospholipase C | calcium |
| M0027 | 3.1.4.3 | phospholipase C | zinc,zinc,zinc |
| M0242 | 3.1.4.41 | sphingomyelin phosphodiesterase D | magnesium |
| M0300 | 3.1.4.46 | glycerophosphodiester phosphodiesterase | magnesium |
| M0158 | 3.1.6.8 | cerebroside-sulfatase | magnesium |
| M0159 | 3.1.8.1 | aryldialkylphosphatase | zinc,zinc |
| M0181 | 3.11.1.1 | phosphonoacetaldehyde hydrolase | magnesium |
| M0019 | 3.2.1.113 | mannosyl-oligosaccharide 1,2-alpha-mannosidase | calcium |
| M0039 | 3.2.2.1 | purine nucleosidase | calcium |
| M0166 | 3.3.2.6 | leukotriene-A4 hydrolase | zinc |
| M0167 | 3.4.11.10 | bacterial leucyl aminopeptidase | zinc,zinc |
| M0168 | 3.4.13.19 | membrane dipeptidase | zinc,zinc |
| M0170 | 3.4.15.1 | peptidyl-dipeptidase A | zinc |
| M0171 | 3.4.17.1 | carboxypeptidase A | zinc |
| M0172 | 3.4.19.- | isoaspartyl dipeptidase | zinc,zinc |
| M0176 | 3.4.24.27 | thermolysin | zinc |
| M0087 | 3.5.1.5 | urease | nickel,nickel |
| M0098 | 3.5.1.88 | peptide deformylase | iron |
| M0258 | 3.5.2.6 | beta-lactamase (Class B) | zinc,zinc |
| M0015 | 3.5.2.6 | beta-lactamase (Class B) | zinc,zinc |
| M0016 | 3.5.2.6 | beta-lactamase (Class B) | zinc |
| M0038 | 3.5.4.16 | GTP cyclohydrolase I | zinc |
| M0097 | 3.5.4.5 | cytidine deaminase | zinc |
| M0284 | 3.5.5.8 | thiocyanate hydrolase | cobalt |
| M0101 | 3.6.1.29 | bis(5'-adenosyl)-triphosphatase | magnesium |
| M0178 | 3.6.3.14 | H+-transporting two-sector ATPase | magnesium |
| M0179 | 3.6.4.9 | chaperonin ATPase | magnesium |
| M0180 | 3.7.1.2 | fumarylacetoacetase | calcium |
| M0215 | 4.1.1.1 | pyruvate decarboxylase | magnesium |
| M0231 | 4.1.1.2 | oxalate decarboxylase | manganese,manganese |
| M0298 | 4.1.1.47 | tartronate-semialdehyde synthase | magnesium |
| M0051 | 4.1.1.49 | phosphoenolpyruvate carboxykinase (ATP) | manganese,magnesium |
| M0220 | 4.1.1.7 | benzoylformate decarboxylase | magnesium |
| M0236 | 4.1.1.85 | 3-dehydro-L-gulonate-6-phosphate decarboxylase | magnesium |
| M0052 | 4.1.2.13 | fructose-bisphosphate aldolase (Class II) | zinc,sodium |
| M0072 | 4.1.2.17 | L-fuculose-phosphate aldolase | zinc |
| M0221 | 4.1.2.38 | benzoin aldolase | magnesium |
| M0272 | 4.1.3.1 | isocitrate lyase | magnesium |
| M0314 | 4.1.3.27 | anthranilate synthase | magnesium |
| M0182 | 4.1.3.30 | methylisocitrate lyase | magnesium |
| M0216 | 4.2.1.1 | carbonate dehydratase | zinc |
| M0311 | 4.2.1.11 | phosphopyruvate hydratase | magnesium,magnesium |
| M0230 | 4.2.1.24 | porphobilinogen synthase | magnesium,zinc |
| M0057 | 4.2.1.84 | nitrile hydratase | iron |
| M0184 | 4.2.2.2 | pectate lyase | calcium |
| M0084 | 4.2.3.12 | 6-pyruvoyltetrahydropterin synthase | zinc,magnesium |
| M0263 | 4.2.3.37 | epi-isozizaene synthase | magnesium,magnesium,magnesium |
| M0059 | 4.2.3.4 | 3-dehydroquinate synthase | zinc |
| M0262 | 4.2.3.6 | trichodiene synthase | magnesium,magnesium,magnesium |
| M0089 | 4.2.3.7 | pentalenene synthase | magnesium |
| M0265 | 4.2.3.9 | aristolochene synthase | magnesium,magnesium,magnesium |
| M0261 | 4.2.3.9 | aristolochene synthase | magnesium,magnesium,magnesium |
| M0032 | 4.4.1.5 | lactoylglutathione lyase | zinc |
| M0058 | 4.6.1.1 | adenylate cyclase | magnesium,magnesium |
| M0297 | 4.99.1.2 | alkylmercury lyase | unknown |
| M0187 | 5.1.2.2 | mandelate racemase | magnesium |
| M0270 | 5.1.3.1 | ribulose-phosphate 3-epimerase | zinc |
| M0273 | 5.1.3.4 | L-ribulose-5-phosphate 4-epimerase | zinc |
| M0033 | 5.1.99.1 | methylmalonyl-CoA epimerase | cobalt |
| M0095 | 5.3.1.3 | arabinose isomerase | manganese |
| M0308 | 5.3.1.5 | xylose isomerase | manganese,manganese |
| M0190 | 5.3.3.2 | isopentenyl-diphosphate delta-isomerase | manganese,magnesium |
| M0206 | 5.4.2.6 | beta-phosphoglucomutase | magnesium |
| M0194 | 5.4.2.8 | phosphomannomutase/phosphoglucomutase | magnesium |
| M0271 | 5.4.2.9 | phosphoenolpyruvate mutase | magnesium |
| M0325 | 5.4.4.2 | isochorismate synthase | magnesium |
| M0063 | 5.4.99.1 | methylaspartate mutase | cobalt |
| M0062 | 5.4.99.2 | methylmalonyl-CoA mutase | cobalt |
| M0269 | 5.5.1.1 | muconate cycloisomerase | manganese |
| M0259 | 5.5.1.8 | bornyl diphosphate synthase | magnesium,magnesium,magnesium |
| M0197 | 6.1.1.1 | tyrosine-tRNA ligase | magnesium |
| M0318 | 6.1.1.17 | glutamate-tRNA ligase | magnesium |
| M0235 | 6.1.1.19 | arginine-tRNA ligase | magnesium |
| M0309 | 6.1.1.5 | isoleucine-tRNA ligase | zinc,zinc,magnesium |
| M0310 | 6.1.2.1 | D-alanine-(R)-lactate ligase | magnesium,magnesium |
| M0198 | 6.2.1.3 | long-chain-fatty-acid-CoA ligase | magnesium |
| M0075 | 6.3.1.1 | aspartate-ammonia ligase | magnesium |
| M0200 | 6.3.1.5 | NAD+ synthase | magnesium,magnesium |
| M0229 | 6.3.2.1 | pantoate-beta-alanine ligase | magnesium |
| M0307 | 6.3.2.19 | Ubiquitin transfer cascade (E1, E2, E3) | magnesium |
| M0199 | 6.3.2.3 | glutathione synthase | magnesium,magnesium |
| M0317 | 6.3.2.9 | UDP-N-acetylmuramoyl-L-alanine---D-glutamate ligase | unknown,unknown |
| M0074 | 6.3.3.3 | dethiobiotin synthase | magnesium,magnesium |
| M0303 | 6.3.3.4 | (carboxyethyl)arginine beta-lactam-synthase | magnesium,magnesium |
| M0065 | 6.3.4.4 | adenylosuccinate synthase | magnesium |
| M0316 | 6.3.4.5 | argininosuccinate synthetase | magnesium |
| M0234 | 6.3.5.2 | GMP synthase (glutamine-hydrolysing) | magnesium |
| M0302 | 6.3.5.4 | asparagine synthase (glutamine-hydrolysing) | magnesium |
| M0223 | 6.4.1.1 | pyruvate carboxylase | zinc,magnesium,magnesium |
| M0201 | 6.4.1.3 | propionyl-CoA carboxylase | magnesium |
| M0202 | 6.5.1.1 | DNA ligase (ATP) | magnesium |
