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Peptidase

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Substrates for peptidase S08.001: subtilisin Carlsberg

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Pharma

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate Uniprot Residue range Cleavage Site Cleavage type Evidence P4 P3 P2 P1 P1' P2' P3' P4' Reference CutDB MERNUM
alpha-2-macroglobulin P01023 24-1474 peptide-Arg719+Leu-peptide P Gly His Ala Arg Leu Val His Val Mortensen et al., 1981
alpha-2-macroglobulin P01023 24-1474 peptide-Leu720+Val-peptide P His Ala Arg Leu Val His Val Glu Mortensen et al., 1981
Antitrypsin P22922 17-392 Peptide-Tyr361+Val-Peptide P NT Ser Lys Val Tyr Val Arg Pro Pro Sasaki et al., 1990
Citrate synthase, mitochondrial O75390 28-466 Peptide-Ala348+Val-Peptide N NT Tyr Gly His Ala Val Leu Arg Lys Lill et al., 1984
Citrate synthase, mitochondrial O75390 28-466 Peptide-Val349+Leu-Peptide N NT Gly His Ala Val Leu Arg Lys Thr Lill et al., 1984
Glucoamylase P69328 25-640 Peptide-Val494+Ala-Peptide N NT Pro Ser Ile Val Ala Thr Gly Gly Stoffer et al., 1993
Glucoamylase P69328 25-640 Peptide-Ser467+Ser-Peptide N NT Thr Ser Ala Ser Ser Val Pro Gly Stoffer et al., 1993
Gly-Leu-Ala-Thr-Gly-Asn-Val-Ser-Thr-Ala-Glu-Leu-Gln-Asp-Ala-Thr-Pro-Ala-NHPhNO2 Gly-Leu-Ala-Thr-Gly-Asn-Val-Ser-Thr-Ala-Glu-Leu-Gln-Asp-Ala-Thr-Pro-Ala+NHPhNO2 S Ala Thr Pro Ala NAN - - - Hauske et al., 2009
hirudin HM2 precursor P81492 21-84 peptide-Gln69+Thr-peptide N Pro Lys Ser Gln Thr Glu Gly Asp Vindigni et al., 1994
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu+Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N Val Glu Ala Leu Tyr Leu Val Cya Toogood et al., 2004
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln+His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N Phe Val Asn Gln His Leu Cya Gly Toogood et al., 2004
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser+His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N Leu Cya Gly Ser His Leu Val Glu Kraus et al., 1976
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu+Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N Gly Ser His Leu Val Glu Ala Leu Kraus et al., 1976
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr+Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N Glu Ala Leu Tyr Leu Val Cya Gly Kraus et al., 1976
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu+Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N Ala Leu Tyr Leu Val Cya Gly Glu Kraus et al., 1976
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr+Thr-Pro-Lys-Ala N Gly Phe Phe Tyr Thr Pro Lys Ala Kraus et al., 1976
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn+Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N - Phe Val Asn Gln His Leu Cya Johansen et al., 1968
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya+Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N MS Tyr Leu Val Cya Gly Glu Arg Gly Kobayashi et al., 2007
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His+Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N MS Val Asn Gln His Leu Cya Gly Ser Kobayashi et al., 2007
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu+Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N MS His Leu Val Glu Ala Leu Tyr Leu Kobayashi et al., 2007
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg+Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N MS Cya Gly Glu Arg Gly Phe Phe Tyr Kobayashi et al., 2007
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe+Phe-Tyr-Thr-Pro-Lys-Ala N MS Glu Arg Gly Phe Phe Tyr Thr Pro Kobayashi et al., 2007
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys+Ala N MS Tyr Thr Pro Lys Ala - - - Kobayashi et al., 2007
Lys-Gly-Lys-Asn-Ser-Gly-Ser-Gly-Ala-Thr-Pro-Val-NHPhNO2 Lys-Gly-Lys-Asn-Ser-Gly-Ser-Gly-Ala-Thr-Pro-Val-NHPhNO2 S Ala Thr Pro Val NAN - - - Hauske et al., 2009
MAP kinase-activated protein kinase 2 P49137 41-364 Peptide-Lys49+Ser-Peptide N MS Phe His Val Lys Ser Gly Leu Gln Tao et al., 2008
MAP kinase-activated protein kinase 2 P49137 41-364 Peptide-Leu220+Thr-Peptide N MS His Asn Ser Leu Thr Thr Pro Cys Tao et al., 2008
MAP kinase-activated protein kinase 2 P49137 41-364 Peptide-Tyr228+Tyr-Peptide N MS Tyr Thr Pro Tyr Tyr Val Ala Pro Tao et al., 2008
MAP kinase-activated protein kinase 2 P49137 41-364 Peptide-Leu269+Ala-Peptide N MS Asn His Gly Leu Ala Ile Ser Pro Tao et al., 2008
myoglobin P68082 2-154 peptide-Leu90+Ala-peptide N Leu Lys Pro Leu Ala Gln Ser His Fontana et al., 1997
myoglobin P68082 2-154 peptide-Gln92+Ser-peptide N Pro Leu Ala Gln Ser His Ala Thr Fontana et al., 1997
myoglobin P68082 2-154 peptide-His94+Ala-peptide N Ala Gln Ser His Ala Thr Lys His Fontana et al., 1997
ribonuclease, pancreatic P61823 27-150 peptide-Met105+Ser-peptide N Tyr Ser Thr Met Ser Ile Thr Asp Polverino de Laureto et al., 1997
ribonuclease, pancreatic P61823 27-150 peptide-Tyr102+Ser-peptide N Tyr Gln Ser Tyr Ser Thr Met Ser Polverino de Laureto et al., 1997
ribonuclease, pancreatic P61823 27-150 peptide-Ala46+Ser-peptide N Thr Ser Ala Ala Ser Ser Ser Asn Richards & Vithayathil, 1959
ribonuclease, pancreatic P61823 27-150 peptide-Ser47+Ser-peptide N Ser Ala Ala Ser Ser Ser Asn Tyr Mendez et al., 2000
ribonuclease, pancreatic P61823 27-150 peptide-Ser42+Thr-peptide N Met Asp Ser Ser Thr Ser Ala Ala Mendez et al., 2000
ribonuclease, pancreatic P61823 27-150 peptide-Ser44+Ala-peptide N Ser Ser Thr Ser Ala Ala Ser Ser Mendez et al., 2000
ribonuclease, pancreatic P61823 27-150 peptide-Ala45+Ala-peptide N Ser Thr Ser Ala Ala Ser Ser Ser Mendez et al., 2000
Ser-Gly-Arg-Val-Val-Pro-Gly-Tyr-Gly-His-Ala-NHPhNO2 Ser-Gly-Arg-Val-Val-Pro-Gly-Tyr-Gly-His-Ala+NHPhNO2 S Tyr Gly His Ala NAN - - - Hauske et al., 2009
serpin {ECO:0000313|EMBL:AEW22118.1} G8UQY8 1-432 peptide-Glu389+Met-peptide P NT Thr Ala Val Glu Met Val Lys Thr Ksiazek et al., 2014
Suc-Ala-Ala-Ala-NHPhNO2 Suc-Ala-Ala-Ala-NHPhNO2 S Suc Ala Ala Ala NAN - - -
Suc-Ala-Ala-Pro-Phe-NHPhNO2 Suc-Ala-Ala-Pro-Phe+NHPhNO2 S Ala Ala Pro Phe NAN - - - Graycar et al., 2004
Z-Gly-Gly-Leu-NHPhNO2 Z-Gly-Gly-Leu+NHPhNO2 S Z Gly Gly Leu NAN - - - Butler, 2004

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