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Literature for peptidase S08.001: subtilisin Carlsberg

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Pharma

(Topics flags: A Assay, S Structure, P Specificity, I Inhibitor, E Expression, V Review, U Therapeutic. To select only the references relevant to a single topic, click the link above. See explanation.)

    2025
  1. D'Almeida,A.P., Goncalves,L.R.B., de Albuqueque da Silva,T.L., Fernandez-Lafuente,R. and Silva,I.J.D., Jr.
    Alcalase immobilization in iota-carrageenan-matrix hydrogel beads derived from the macroalga Solieria filiformis
    Enzyme Microb Technol (2025) 188, 110636-110636. PubMed  Europe PubMed DOI
  2. Di,J., Li,Y., Zhang,Y., Goh,K.L. and Zheng,M.
    Enzymatic synthesis of antioxidant peptides with controllable and adjustable molecular weights using magnetically recyclable immobilized Alcalase
    Int J Biol Macromol (2025) 306, 141473-141473. PubMed  Europe PubMed DOI
  3. Khan,H., Ul Haq,I., Khan,Z., Nughman,M., Rehman,Z.U., Khan,T.A., Khan,S., Allahyani,M., Alsiwiehri,N., Alshamrani,M.A., Shehzad,A. and Muhammad,N.
    Enhanced Stability and Reusability of Subtilisin Carlsberg Through Immobilization on Magnetic Nanoparticles
    Nanotechnol Sci Appl (2025) 18, 71-91. PubMed  Europe PubMed DOI  PMC  EPMC
  4. Terron-Hernandez,J., Gomez-Velasco,H., Pinzon-Yaya,L., Hernandez-Santoyo,A., Garcia-Ramirez,B. and Rodriguez-Romero,A.
    Understanding the structure and function of HPI, a rubber tree serine protease inhibitor, and its interaction with subtilisin
    Biochem Biophys Res Commun (2025) 763, 151801-151801. PubMed  Europe PubMed DOI
  5. Yang,Y., Zhang,B. and Zhang,J.
    Dynamic catalytic domain plasticity governs substrate specificity in industrial serine proteases: Structural and functional implications
    Int J Biol Macromol (2025) 331, 148374-148374. PubMed  Europe PubMed DOI
    6. 2024
  6. Jiang,X., Yeung,D., Liu,Y., Spicer,V., Afshari,H., Lao,Y., Lin,F., Krokhin,O. and Zahedi,R.P.
    Accelerating Proteomics Using Broad Specificity Proteases
    J Proteome Res (2024) 23, 1360-1369. PubMed  Europe PubMed DOI
  7. Shettar,S.S., Bagewadi,Z.K., Yunus Khan,T.M., Mohamed Shamsudeen,S. and Kolvekar,H.N.
    Biochemical characterization of immobilized recombinant subtilisin and synthesis and functional characterization of recombinant subtilisin capped silver and zinc oxide nanoparticles
    Saudi J Biol Sci (2024) 31, 104009-104009. PubMed  Europe PubMed DOI  PMC  EPMC
  8. Wei,S., Li,L., Lu,P., Suzuki,M., Okuda,S., Okamoto,K., Itoh,H. and Nagata,K.
    Identification of a novel subtilisin-derived peptide, SC-(1-31), with cytotoxic activity
    Biochem Biophys Res Commun (2024) 742, 151101-151101. PubMed  Europe PubMed DOI
  9. Yu,X., Li,Y., Qian,Z., Wei,L., Xie,J., Tong,M. and Zhang,Y.
    Protein engineering of an alkaline protease from Bacillus licheniformis (BLAP) for efficient and specific chiral resolution of the racemic ethyl tetrahydrofuroate
    Enzyme Microb Technol (2024) 181, 110523-110523. PubMed  Europe PubMed DOI
    10. 2023
  10. Eser,A. and Aydemir,T.
    Immobilization of Subtilisin Carlsberg and its use for transesterification of N-acetyl-L-phenylalanine ethyl ester in organic medium
    Bioprocess Biosyst Eng (2023) 46, 1147-1162. PubMed  Europe PubMed DOI
  11. Falkenberg,F., Kohn,S., Bott,M., Bongaerts,J. and Siegert,P.
    Biochemical characterisation of a novel broad pH spectrum subtilisin from Fictibacillus arsenicus DSM 15822(T)
    FEBS Open Bio (2023) 13, 2035-2046. PubMed  Europe PubMed DOI  PMC  EPMC
  12. Liu,X., Huang,Q., Pan,P., Fang,M., Zhang,Y., Yang,S., Li,M. and Liu,Y.
    Comparative Study of the Preparation of High-Molecular-Weight Fibroin by Degumming Silk with Several Neutral Proteases
    Polymers (Basel) (2023) 15 PubMed  Europe PubMed DOI  PMC  EPMC
  13. Rahimnahal,S., Meimandipour,A., Fayazi,J., Asghar Karkhane,A., Shamsara,M., Beigi Nassiri,M., Mirzaei,H., Hamblin,M.R., Tarrahimofrad,H., Bakherad,H., Zamani,J. and Mohammadi,Y.
    Biochemical and molecular characterization of novel keratinolytic protease from Bacillus licheniformis (KRLr1)
    Front Microbiol (2023) 14, 1132760-1132760. PubMed  Europe PubMed DOI  PMC  EPMC
  14. Rojas,L., Cabrera-Munoz,A., Espinosa,L.A., Montane,S., Alvarez-Lajonchere,L., Mojarena,J.D., Moya,G., Lorenzo,J., Gonzalez,L.J., Betzel,C. and Alonso-Del-Rivero Antigua,M.
    CogiTx1: A novel subtilisin A inhibitor isolated from the sea anemone Condylactis gigantea belonging to the defensin 4 protein family
    Biochimie (2023) 213, 41-53. PubMed  Europe PubMed DOI
    15. 2022
  15. Azrin,N.A.M., Ali,M.S.M., Rahman,R.N.Z.R., Oslan,S.N. and Noor,N.D.M.
    Versatility of subtilisin: A review on structure, characteristics, and applications
    Biotechnol Appl Biochem (2022) 69, 2599-2616. PubMed  Europe PubMed DOI
  16. Hu,J., Chang,R., Yuan,Y., Li,Z. and Wang,Y.
    Identification of Key Residues Essential for the Activation of Plant Immunity by Subtilisin From Bacillus velezensis LJ02
    Front Microbiol (2022) 13, 869596-869596. PubMed  Europe PubMed DOI  PMC  EPMC
    17. 2020
  17. Gong,J.S., Ye,J.P., Tao,L.Y., Su,C., Qin,J., Zhang,Y.Y., Li,H., Li,H., Xu,Z.H. and Shi,J.S.
    Efficient keratinase expression via promoter engineering strategies for degradation of feather wastes
    Enzyme Microb Technol (2020) 137, 109550-109550. PubMed  Europe PubMed DOI
  18. Haq,I.U., Akram,F. and Jabbar,Z.
    Keratinolytic enzyme-mediated biodegradation of recalcitrant poultry feathers waste by newly isolated Bacillus sp. NKSP-7 under submerged fermentation
    Folia Microbiol (Praha) (2020) PubMed  Europe PubMed DOI
  19. Wang,H., Song,P., Li,X., Wang,Y., Gui,S., Liu,Y. and Lu,F.
    Screening of the candidate inhibitory peptides of subtilisin by in vitro RNA display technique
    Int J Biol Macromol (2020) 163, 1162-1167. PubMed  Europe PubMed DOI
  20. Yu,J., Smith,I.N., Idris,N., Gregory,N. and Mikiashvili,N.
    Oxidative Stability of Protease Treated Peanut with Reduced Allergenicity
    Foods (2020) 9 PubMed  Europe PubMed DOI  PMC  EPMC
    21. 2019
  21. Alavi,F., Jamshidian,M. and Rezaei,K.
    Applying native proteases from melon to hydrolyze kilka fish proteins (Clupeonella cultriventris caspia) compared to commercial enzyme Alcalase
    Food Chem (2019) 277, 314-322. PubMed  Europe PubMed DOI
  22. Cabrera-Munoz,A., Valiente,P.A., Rojas,L., Alonso-Del-Rivero Antigua,M. and Pires,J.R.
    NMR structure of CmPI-II, a non-classical Kazal protease inhibitor: understanding its conformational dynamics and subtilisin A inhibition
    J Struct Biol (2019) 206, 280-294. PubMed  Europe PubMed DOI  S
  23. Cao,S., Li,D., Ma,X., Xin,Q., Song,J., Lu,F. and Li,Y.
    A novel unhairing enzyme produced by heterologous expression of keratinase gene (kerT) in Bacillus subtilis
    World J Microbiol Biotechnol (2019) 35, 122-122. PubMed  Europe PubMed DOI
  24. Rekik,H., Frikha,F., Zarai Jaouadi,N., Gargouri,F., Jmal,N., Bejar,S. and Jaouadi,B.
    Gene cloning, expression, molecular modeling and docking study of the protease SAPRH from Bacillus safensis strain RH12
    Int J Biol Macromol (2019) 125, 876-891. PubMed  Europe PubMed DOI
    25. 2018
  25. Dorau,R., Gorbe,T. and Svedendahl Humble,M.
    Improved enantioselectivity of subtilisin Carlsberg towards secondary alcohols by protein engineering
    Chembiochem (2018) 19, 338-346. PubMed  Europe PubMed DOI
  26. Hwang,H.J., Kim,Y.T., Kang,N.S. and Han,J.W.
    A simple method for removal of the Chlamydomonas reinhardtii cell wall using a commercially available subtilisin (Alcalase)
    J Mol Microbiol Biotechnol (2018) 28, 169-178. PubMed  Europe PubMed DOI
    27. 2017
  27. Fang,Z., Yong,Y.C., Zhang,J., Du,G. and Chen,J.
    Keratinolytic protease: a green biocatalyst for leather industry
    Appl Microbiol Biotechnol (2017) 101, 7771-7779. PubMed  Europe PubMed DOI
  28. Huang,M., Chen,R. and Ren,G.
    Secretory expression and purification of Bacillus licheniformis keratinase in insect cells
    PLoS ONE (2017) 12, e0183764-e0183764. PubMed  Europe PubMed DOI  E
  29. Prasad,S. and Roy,I.
    Obtaining a high activity subtilisin preparation by controlled thermal stress in n-octane
    Anal Biochem (2017) 534, 86-90. PubMed  Europe PubMed DOI
  30. Visetnan,S., Supungul,P., Tassanakajon,A., Donpudsa,S. and Rimphanitchayakit,V.
    A single WAP domain-containing protein from Litopenaeus vannamei possesses antiproteinase activity against subtilisin and antimicrobial activity against AHPND-inducing Vibrio parahaemolyticus
    Fish Shellfish Immunol (2017) 68, 341-348. PubMed  Europe PubMed DOI
    31. 2016
  31. Wei,G., Tian,N., Siezen,R., Schuppan,D. and Helmerhorst,E.J.
    Identification of food-grade subtilisins as gluten-degrading enzymes to treat celiac disease
    Am J Physiol Gastrointest Liver Physiol (2016) 311, G571-G580. PubMed  Europe PubMed DOI
  32. Zhang,L., Li,Y., Yuan,Y., Jiang,Y., Guo,Y., Li,M. and Pu,X.
    Molecular mechanism of carbon nanotube to activate subtilisin Carlsberg in polar and non-polar organic media
    Sci Rep (2016) 6, 36838-36838. PubMed  Europe PubMed DOI  PMC  EPMC
    33. 2015
  33. Abdizadeh,H., Guven,G., Atilgan,A.R. and Atilgan,C.
    Perturbation response scanning specifies key regions in subtilisin serine protease for both function and stability
    J Enzyme Inhib Med Chem (2015) 30, 867-873. PubMed  Europe PubMed DOI  S
  34. Madonov,P.G., Kinsht,D.N., Ershov,K.I., Shilova,M.A. and Solov'ev,O.N.
    [Experience with clinical administration of new drug Thrombovasim(R) in vascular surgery]
    Angiol Sosud Khir (2015) 21, 99-104. PubMed  Europe PubMed  U
    35. 2014
  35. Kasper,J.R., Andrews,E.C. and Park,C.
    Product inhibition in native-state proteolysis
    PLoS ONE (2014) 9, e111416-e111416. PubMed  Europe PubMed DOI  PMC  EPMC
  36. Liu,B., Zhang,J., Gu,L., Du,G., Chen,J. and Liao,X.
    Comparative analysis of bacterial expression systems for keratinase production
    Appl Biochem Biotechnol (2014) 173, 1222-1235. PubMed  Europe PubMed DOI  E
    37. 2013
  37. Despotovic,D., Vojcic,L., Blanusa,M., Maurer,K.H., Zacharias,M., Bocola,M., Martinez,R. and Schwaneberg,U.
    Redirecting catalysis from proteolysis to perhydrolysis in subtilisin Carlsberg
    J Biotechnol (2013) 167, 279-286. PubMed  Europe PubMed DOI
  38. Hu,H., He,J., Yu,B., Zheng,P., Huang,Z., Mao,X., Yu,J., Han,G. and Chen,D.
    Expression of a keratinase (kerA) gene from Bacillus licheniformis in Escherichia coli and characterization of the recombinant enzymes
    Biotechnol Lett (2013) 35, 239-244. PubMed  Europe PubMed DOI
  39. Hu,H., Gao,J., He,J., Yu,B., Zheng,P., Huang,Z., Mao,X., Yu,J., Han,G. and Chen,D.
    Codon optimization significantly improves the expression level of a keratinase gene in Pichia pastoris
    PLoS ONE (2013) 8, e58393-e58393. PubMed  Europe PubMed DOI  PMC  EPMC  E
  40. Liu,B., Zhang,J., Fang,Z., Gu,L., Liao,X., Du,G. and Chen,J.
    Enhanced thermostability of keratinase by computational design and empirical mutation
    J Ind Microbiol Biotechnol (2013) 40, 697-704. PubMed  Europe PubMed DOI
  41. Okoroma,E.A., Purchase,D., Garelick,H., Morris,R., Neale,M.H., Windl,O. and Abiola,O.O.
    Enzymatic formulation capable of degrading scrapie prion under mild digestion conditions
    PLoS ONE (2013) 8, e68099-e68099. PubMed  Europe PubMed DOI  PMC  EPMC
  42. Trillo-Muyo,S., Martinez-Rodriguez,S., Arolas,J.L. and Gomis-Ruth,F.X.
    Mechanism of action of a Janus-faced single-domain protein inhibitor simultaneously targeting two peptidase classes
    Chem Sci (2013) 4, 791-797. DOI  S
    43. 2012
  43. Fuchita,N., Arita,S., Ikuta,J., Miura,M., Shimomura,K., Motoshima,H. and Watanabe,K.
    Gly or Ala substitutions for Pro(210)Thr(211)Asn(212) at the beta8-beta9 turn of subtilisin Carlsberg increase the catalytic rate and decrease thermostability
    Biochim Biophys Acta (2012) 1824, 620-626. PubMed  Europe PubMed DOI
  44. Gabor,E., Niehaus,F., Aehle,W. and Eck,J.
    Zooming In on metagenomics: molecular microdiversity of subtilisin Carlsberg in soil
    J Mol Biol (2012) 418, 16-20. PubMed  Europe PubMed DOI
  45. Ghasemi,Y., Dabbagh,F. and Ghasemian,A.
    Cloning of a fibrinolytic enzyme (subtilisin) gene from Bacillus subtilis in Escherichia coli
    Mol Biotechnol (2012) 52, 1-7. PubMed  Europe PubMed DOI  E
  46. Graycar,T.P., Bott,R.R., Power,S.D. and Estell,D.A.
    Subtilisins
    [ISSN:978-0-12-407742-3] (2012) 3, 3148-3155. DOI
  47. Mironczuk,A.M., Krasowsk,A., Murzyn,A., Plachetka,M. and Lukaszewicz,M.
    Production of the Bacillus licheniformis subC protease using Lactococcus lactis NICE expression system
    SpringerPlus (2012) 1, 54-54. PubMed  Europe PubMed DOI  E
  48. Petrillo,T., O'Donohoe,C.A., Howe,N. and Malthouse,J.P.
    Importance of tetrahedral intermediate formation in the catalytic mechanism of the serine proteases chymotrypsin and subtilisin
    Biochemistry (2012) 51, 6164-6170. PubMed  Europe PubMed DOI  I
  49. Rajput,R., Tiwary,E., Sharma,R. and Gupta,R.
    Swapping of pro-sequences between keratinases of Bacillus licheniformis and Bacillus pumilus: altered substrate specificity and thermostability
    Enzyme Microb Technol (2012) 51, 131-138. PubMed  Europe PubMed DOI
    50. 2011
  50. Addae,J.I. and Stone,T.W.
    The serine protease subtilisin suppresses epileptiform activity in rat hippocampal slices and neocortex in vivo
    Neuroscience (2011) 199, 64-73. PubMed  Europe PubMed DOI
  51. Forrest,C.M., Addae,J.I., Murthy,S., Darlington,L.G., Morris,B.J. and Stone,T.W.
    Molecular changes associated with hippocampal long-lasting depression induced by the serine protease subtilisin-A
    Eur J Neurosci (2011) 34, 1241-1253. PubMed  Europe PubMed DOI
  52. Lousa,D., Baptista,A.M. and Soares,C.M.
    Structural determinants of ligand imprinting: a molecular dynamics simulation study of subtilisin in aqueous and apolar solvents
    Protein Sci (2011) 20, 379-386. PubMed  Europe PubMed DOI
  53. Silvestre-Ryan,J., Lin,Y. and Chu,J.W.
    "Fluctuograms" reveal the intermittent intra-protein communication in subtilisin Carlsberg and correlate mechanical coupling with co-evolution
    PLoS Comput Biol (2011) 7, e1002023-e1002023. PubMed  Europe PubMed DOI  PMC  EPMC
    54. 2010
  54. Cianci,M., Tomaszewski,B., Helliwell,J.R. and Halling,P.J.
    Crystallographic analysis of counterion effects on subtilisin enzymatic action in acetonitrile
    J Am Chem Soc (2010) 132, 2293-2300. PubMed  Europe PubMed DOI
  55. Falcioni,F., Housden,H.R., Ling,Z., Shimizu,S., Walker,A.J. and Bruce,N.C.
    Soluble, folded and active subtilisin in a protic ionic liquid
    Chem Commun (Camb) (2010) 46, 749-751. PubMed  Europe PubMed DOI
  56. Li,H.H., He,Y.H. and Guan,Z.
    Protease-catalyzed direct aldol reaction
    Catal Commun (2010) 12, 580-582. DOI
  57. Sung,J.H., Ahn,S.J., Kim,N.Y., Jeong,S.K., Kim,J.K., Chung,J.K. and Lee,H.H.
    Purification, molecular cloning, and biochemical characterization of subtilisin JB1 from a newly isolated Bacillus subtilis JB1
    Appl Biochem Biotechnol (2010) 162, 900-911. PubMed  Europe PubMed DOI
  58. Tiwary,E. and Gupta,R.
    Medium optimization for a novel 58 kDa dimeric keratinase from Bacillus licheniformis ER-15: biochemical characterization and application in feather degradation and dehairing of hides
    Bioresour Technol (2010) 101, 6103-6110. PubMed  Europe PubMed DOI
  59. Tiwary,E. and Gupta,R.
    Extracellular expression of keratinase from Bacillus licheniformis ER-15 in Escherichia coli
    J Agric Food Chem (2010) 58, 8380-8385. PubMed  Europe PubMed DOI  E
    60. 2009
  60. Fakhfakh,N., Kanoun,S., Manni,L. and Nasri,M.
    Production and biochemical and molecular characterization of a keratinolytic serine protease from chicken feather-degrading Bacillus licheniformis RPk
    Can J Microbiol (2009) 55, 427-436. PubMed  Europe PubMed DOI
  61. Howe,N., Rogers,L., Hewage,C. and Malthouse,J.P.
    Oxyanion and tetrahedral intermediate stabilisation by subtilisin: detection of a new tetrahedral adduct
    Biochim Biophys Acta (2009) 1794, 1251-1258. PubMed  Europe PubMed DOI  I
  62. Pagan,M., Sola,R.J. and Griebenow,K.
    On the role of protein structural dynamics in the catalytic activity and thermostability of serine protease subtilisin Carlsberg
    Biotechnol Bioeng (2009) 103, 77-84. PubMed  Europe PubMed DOI  PMC  EPMC
  63. Pietrusewicz,E., Sienczyk,M. and Oleksyszyn,J.
    Novel diphenyl esters of peptidyl alpha-aminoalkylphosphonates as inhibitors of chymotrypsin and subtilisin
    J Enzyme Inhib Med Chem (2009) 24, 1229-1236. PubMed  Europe PubMed DOI  I
    64. 2008
  64. Castillo,B., Sola,R.J., Ferrer,A., Barletta,G. and Griebenow,K.
    Effect of PEG modification on subtilisin Carlsberg activity, enantioselectivity, and structural dynamics in 1,4-dioxane
    Biotechnol Bioeng (2008) 99, 9-17. PubMed  Europe PubMed DOI
  65. Ruan,B., London,V., Fisher,K.E., Gallagher,D.T. and Bryan,P.N.
    Engineering substrate preference in subtilisin: structural and kinetic analysis of a specificity mutant
    Biochemistry (2008) 47, 6628-6636. PubMed  Europe PubMed DOI  P
    66. 2007
  66. Radha,S. and Gunasekaran,P.
    Cloning and expression of keratinase gene in Bacillus megaterium and optimization of fermentation conditions for the production of keratinase by recombinant strain
    J Appl Microbiol (2007) 103, 1301-1310. PubMed  Europe PubMed DOI
    67. 2004
  67. Graycar,T.P., Ballinger,M.D. and Wells,J.A.
    Subtilisins
    [ISSN:0-12-079610-4] (2004) 2, 1786-1792.  V
  68. [YEAR:23-11-2004]Ruan,B., Fisher,K.E., Alexander,P.A., Doroshko,V. and Bryan,P.N.
    Engineering subtilisin into a fluoride-triggered processing protease useful for one-step protein purification
    Biochemistry (23-11-2004) 43, 14539-14546. PubMed  Europe PubMed DOI
    69. 2003
  69. Mei,H.C., Li,Y.F., Hsu,C.C., Tsai,Y.C. and Takagi,H.
    Conversion of the cleavage specificity of subtilisin YaB on oxidized insulin chains to an elastase-like specificity by replacement of Gly124 with Ala
    Biosci Biotechnol Biochem (2003) 67, 1601-1604. PubMed  Europe PubMed DOI
  70. Pan,X., Bott,R. and Glatz,C.E.
    Subtilisin surface properties and crystal growth kinetics
    J Cryst Growth (2003) 254, 492-502. DOI  S
    71. 1998
  71. Stoll,V.S., Eger,B.T., Hynes,R.C., Martichonok,V., Jones,J.B. and Pai,E.F.
    Differences in binding modes of enantiomers of 1-acetamido boronic acid based protease inhibitors: crystal structures of gamma-chymotrypsin and subtilisin Carlsberg complexes
    Biochemistry (1998) 37, 451-462. PubMed  Europe PubMed DOI  S  I
    72. 1997
  72. Dahlen,J.R., Foster,D.C. and Kisiel,W.
    Human proteinase inhibitor 9 (PI9) is a potent inhibitor of subtilisin A
    Biochem Biophys Res Commun (1997) 238, 329-333. PubMed  Europe PubMed DOI
  73. Huang,H.W., Chen,W.C., Wu,C.Y., Yu,H.C., Lin,W.Y., Chen,S.T. and Wang,K.T.
    Kinetic studies of the inhibitory effects of propeptides subtilisin BPN' and Carlsberg to bacterial serine proteases
    Protein Eng (1997) 10, 1227-1233. PubMed  Europe PubMed
    74. 1996
  74. Tang,L.X., Rowell,F.J. and Cumming,R.H.
    A rapid homogeneous fluorescence assay for subtilisin
    Anal Lett (1996) 29, 2085-2095.  A
    75. 1995
  75. Lin,X., Kelemen,D.W., Miller,E.S. and Shih,J.C.
    Nucleotide sequence and expression of kerA, the gene encoding a keratinolytic protease of Bacillus licheniformis PWD-1
    Appl Environ Microbiol (1995) 61, 1469-1474. PubMed  Europe PubMed  PMC  EPMC
    76. 1994
  76. [YEAR:30-8-1994]Steinmetz,A.C., Demuth,H.U. and Ringe,D.
    Inactivation of subtilisin Carlsberg by N-((tert-butoxycarbonyl)alanylprolylphenylalanyl)-O-benzoylhydroxylamine: formation of a covalent enzyme-inhibitor linkage in the form of a carbamate derivative
    Biochemistry (30-8-1994) 33, 10535-10544. PubMed  Europe PubMed  S  I
    77. 1993
  77. [YEAR:21-12-1993]Bell,I.M. and Hilvert,D.
    Peroxide dependence of the semisynthetic enzyme selenosubtilisin
    Biochemistry (21-12-1993) 32, 13969-13973. PubMed  Europe PubMed DOI
  78. Stoffer,B., Frandsen,T.P., Busk,P.K., Schneider,P., Svendsen,I. and Svensson,B.
    Production, purification and characterization of the catalytic domain of glucoamylase from Aspergillus niger
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    79. 1992
  79. Graycar,T.P., Bott,R.R., Caldwell,R.M., Dauberman,J.L., Lad,P.J., Power,S.D., Sagar,I.H., Silva,R.A., Weiss,G.L., Woodhouse,L.R. and Estell,D.A.
    Altering the proteolytic activity of subtilisin through protein engineering
    Ann N Y Acad Sci (1992) 672, 71-79. PubMed  Europe PubMed
  80. Stambolieva,N.A., Ivanov,I.P. and Yomtova,V.M.
    N-Anthraniloyl-Ala-Ala-Phe-4-nitroanilide, a highly sensitive substrate for subtilisins
    Arch Biochem Biophys (1992) 294, 703-706. PubMed  Europe PubMed DOI
    81. 1991
  81. Meldal,M. and Breddam,K.
    Anthranilamide and nitrotyrosine as a donor-acceptor pair in internally quenched fluorescent substrates for endopeptidases: multicolumn peptide synthesis of enzyme substrates for subtilisin Carlsberg and pepsin
    Anal Biochem (1991) 195, 141-147. PubMed  Europe PubMed
    82. 1990
  82. Wong,C.H., Chen,S.T., Hennen,W.J., Bibbs,J.A., Wang,Y.F., Liu,J.L.C., Pantoliano,M.W., Whitlow,M. and Bryan,P.N.
    Enzymes in organic synthesis: use of subtilisin and a highly stable mutant derived from multiple site-specific mutations
    J Am Chem Soc (1990) 112, 945-953.
    83. 1988
  83. [YEAR:15-9-1988]Ikemura,H. and Inouye,M.
    In vitro processing of pro-subtilisin produced in Escherichia coli
    J Biol Chem (15-9-1988) 263, 12959-12963. PubMed  Europe PubMed  E
  84. McPhalen,C.A. and James,M.N.
    Structural comparison of two serine proteinase-protein inhibitor complexes: eglin-c-subtilisin Carlsberg and CI-2-subtilisin Novo
    Biochemistry (1988) 27, 6582-6598. PubMed  Europe PubMed  S
  85. Neidhart,D.J. and Petsko,G.A.
    The refined crystal structure of subtilisin Carlsberg at 2.5 A resolution
    Protein Eng (1988) 2, 271-276. PubMed  Europe PubMed  S
  86. Wells,J.A. and Estell,D.A.
    Subtilisin - an enzyme designed to be engineered
    Trends Biochem Sci (1988) 13, 291-297. PubMed  Europe PubMed
    87. 1987
  87. Bode,W., Papamokos,E. and Musil,D.
    The high-resolution X-ray crystal structure of the complex formed between subtilisin Carlsberg and eglin c, an elastase inhibitor from the leech Hirudo medicinalis. Structural analysis, subtilisin structure and interface geometry
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