$VAR1 = undef;

Summary for peptidase S08.001: subtilisin Carlsberg

Names
MEROPS Name	subtilisin Carlsberg
Other names	Alcalase Novo, kerA g.p. (Bacillus licheniformis), Ker BL (Bacillus licheniformis), Ker BP (Bacillus pumilus), keratinase (Bacillus sp.), subtilisin A, subtilisin JB1, subtilopeptidase A, thiolsubtilisin, Thrombovasim

Domain architecture

MEROPS Classification
Classification	Clan SB >> Subclan (none) >> Family S8 >> Subfamily A >> S08.001
Holotype	subtilisin Carlsberg (Bacillus licheniformis), Uniprot accession P00780 (peptidase unit: 106-379), MERNUM MER0000309
History	Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.

Activity
Catalytic type	Serine
Peplist	Included in the Peplist with identifier PL00342
NC-IUBMB	Subclass 3.4 (Peptidases) >> Sub-subclass 3.4.21 (Serine endopeptidases) >> Peptidase 3.4.21.62
Enzymology	BRENDA database
Biotechnology	Forms of subtilisin are widely used commercially.
Cleavage site specificity	Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern	-/-/-/-as/-/-/- (based on 43 cleavages)

Specificity matrix

Amino acid	P4	P3	P2	P1	P1'	P2'	P3'	P4'
Gly	4	4	4	0	2	1	4	5
Pro	3	0	6	0	0	1	3	4
Ala	5	6	8	6	8	1	4	2
Val	2	1	6	3	5	5	3	1
Leu	2	4	1	8	4	5	2	2
Ile	0	0	1	0	0	2	0	0
Met	1	0	0	1	1	0	1	0
Phe	2	2	1	2	1	1	1	0
Tyr	7	0	1	5	2	1	1	2
Trp	0	0	0	0	0	0	0	0
Ser	4	6	6	5	7	4	5	4
Thr	3	5	2	0	4	4	2	3
Cys	0	0	0	0	0	0	0	1
Asn	1	2	1	1	0	0	1	1
Gln	0	2	1	3	1	1	0	1
Asp	0	1	0	0	0	0	0	2
Glu	2	1	1	2	0	3	0	3
Lys	0	3	0	2	0	0	4	1
Arg	0	1	1	2	0	2	2	0
His	3	4	3	2	2	3	1	2

Specificity from combinatorial peptides

Organism	comment	P4	P3	P2	P1	P1'	P2'	P3'	P4'	optimal substrate	fluorophore or acceptor-donor pair	Reference
Bacillus licheniformis	wild type	-	-	-	K	-	-	-	-	xxxKxxxx	ACC-Dabcyl	Sun et al., 2007
Bacillus licheniformis	wild type	V/M/F	Q	P	L	D	E	-	-	VQPLDExx	Abz-Tyr(NO2)	Meldal et al., 1991