Substrates for peptidase M01.001: aminopeptidase N

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Pharma

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference
(Asn1)angiotensin-2			Asn+Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu	S	-	-	-	Asn	Arg	Val	Tyr	Ile	Ward et al., 1990
Ala-NHMec			Ala+NHMec	S	-	-	-	Ala	AMC	-	-	-	Turner, 2004
Ala-NHMec			Ala+NHMec	S	-	-	-	Ala	AMC	-	-	-	Monod et al., 2005
Ala-NHNap			Ala+NHNap	S	-	-	-	Ala	NAP	-	-	-	Turner, 2004
Ala-NHPhNO2			Ala+NHPhNO2	S	-	-	-	Ala	NAN	-	-	-	Turner, 2004
Ala-Phe			Ala+Phe	S	-	-	-	Ala	Phe	-	-	-	Gee & Kenny, 1987
Ala-Trp			Ala+Trp	S	-	-	-	Ala	Trp	-	-	-	Gee & Kenny, 1987
angiotenin-3	P01019	35-41	Arg+Val-Tyr-Ile-His-Pro-Phe	P	-	-	-	Arg	Val	Tyr	Ile	His	Ward et al., 1990
angiotensin-3	P01019	35-41	Arg35+Val-peptide	P	-	-	-	Arg	Val	Tyr	Ile	His	Vaghy et al., 1995
Arg-NHMec			Arg+NHMec	S	-	-	-	Arg	AMC	-	-	-	Monod et al., 2005
Asn-NHMec			Asn+NHMec	S	-	-	-	Asn	AMC	-	-	-	Monod et al., 2005
Cys-Gly			Cys+Gly	P	-	-	-	Cys	Gly	-	-	-	Turner, 2004
Cys-NHMec			Cys+NHMec	S	-	-	-	Cys	AMC	-	-	-	Monod et al., 2005
des(Asp1)angiotensin-1	P01019	35-43	Arg+Val-Tyr-Ile-His-Pro-Phe-His-Leu	N	-	-	-	Arg	Val	Tyr	Ile	His	Ward et al., 1990
Gln-NHMec			Gln+NHMec	S	-	-	-	Gln	AMC	-	-	-	Monod et al., 2005
Glu-Trp			Glu+Trp	S	-	-	-	Glu	Trp	-	-	-	Gee & Kenny, 1987
Gly-NHMec			Gly+NHMec	S	-	-	-	Gly	AMC	-	-	-	Monod et al., 2005
Gly-Trp			Gly+Trp	S	-	-	-	Gly	Trp	-	-	-	Gee & Kenny, 1987
Gly-Tyr			Gly+Tyr	S	-	-	-	Gly	Tyr	-	-	-	Gee & Kenny, 1987
hepta(5-11)substance P	P20366	62-68	Gln+Gln-Phe-Phe-Gly-Leu-Met-NH2	N	-	-	-	Gln	Gln	Phe	Phe	Gly	Ward et al., 1990
His-NHMec			His+NHMec	S	-	-	-	His	AMC	-	-	-	Monod et al., 2005
Leu-NHMec			Leu+NHMec	S	-	-	-	Leu	AMC	-	-	-	Turner, 2004
Leu-NHMec			Leu+NHMec	S	-	-	-	Leu	AMC	-	-	-	Monod et al., 2005
Leu-Phe			Leu+Phe	S	-	-	-	Leu	Phe	-	-	-	Gee & Kenny, 1987
Leu-Trp			Leu+Trp	S	-	-	-	Leu	Trp	-	-	-	Gee & Kenny, 1987
Leu-Tyr			Leu+Tyr	S	-	-	-	Leu	Tyr	-	-	-	Gee & Kenny, 1987
Lys-NHMec			Lys+NHMec	S	-	-	-	Lys	AMC	-	-	-	Monod et al., 2005
Met-NHMec			Met+NHMec	S	-	-	-	Met	AMC	-	-	-	Monod et al., 2005
neurokinin analogue 1			Arg+Phe-Phe-Sar-Leu-Met-O2	S	-	-	-	Arg	Phe	Phe	Sar	Leu	Ward et al., 1990
Phe-Gly			Phe+Gly	S	-	-	-	Phe	Gly	-	-	-	Gee & Kenny, 1987
Phe-Leu			Phe+Leu	S	-	-	-	Phe	Leu	-	-	-	Gee & Kenny, 1987
Phe-NHMec			Phe+NHMec	S	-	-	-	Phe	AMC	-	-	-	Monod et al., 2005
Pro-Phe			Pro+Phe	S	-	-	-	Pro	Phe	-	-	-	Gee & Kenny, 1987
Pro-Trp			Pro+Trp	S	-	-	-	Pro	Trp	-	-	-	Gee & Kenny, 1987
Ser-NHMec			Ser+NHMec	S	-	-	-	Ser	AMC	-	-	-	Monod et al., 2005
somatostatin	P61278	103-116	Ala+Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Phe-Thr-Phe-Ser-Cys	P	-	-	-	Ala	Gly	Cys	Lys	Asn	Ward et al., 1990
Trp-Leu			Trp+Leu	S	-	-	-	Trp	Leu	-	-	-	Gee & Kenny, 1987
Trp-Trp			Trp+Trp	S	-	-	-	Trp	Trp	-	-	-	Gee & Kenny, 1987
Tyr-Leu			Tyr+Leu	S	-	-	-	Tyr	Leu	-	-	-	Gee & Kenny, 1987
Val-Leu			Val+Leu	S	-	-	-	Val	Trp	-	-	-	Gee & Kenny, 1987
[Leu5]-enkephalin	P01213	175-179	Tyr+Gly-Gly-Phe-Leu-NH2	N	-	-	-	Tyr	Gly	Gly	Phe	Leu	Turner, 2004
[Met5]-enkephalin	P01189	237-241	Tyr+Gly-Gly-Phe-Leu-NH2	N	-	-	-	Tyr	Gly	Gly	Phe	Met	Turner, 2004