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Peptidase

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Substrates for peptidase C01.001: papain

Summary Alignment Sequences Sequence features Distribution Structure Literature Substrates Pharma

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate Uniprot Residue range Cleavage Site Cleavage type Evidence P4 P3 P2 P1 P1' P2' P3' P4' Reference CutDB MERNUM
Abz-Ala-Arg-Gln-Gln-Tyr(NO2)-Asp Abz-Ala-Arg+Gln-Gln-Tyr(NO2)-Asp S - Abz Ala Arg Gln Gln Tyn Asp Than et al., 2004
Abz-Arg-Arg-Gln-Gln-Tyr(NO2)-Asp Abz-Arg-Arg+Gln-Gln-Tyr(NO2)-Asp S - Abz Arg Arg Gln Gln Tyn Asp Than et al., 2004
Abz-Asn-Arg-Gln-Gln-Tyr(NO2)-Asp Abz-Asn-Arg+Gln-Gln-Tyr(NO2)-Asp S - Abz Asn Arg Gln Gln Tyn Asp Than et al., 2004
Abz-Asp-Arg-Gln-Gln-Tyr(NO2)-Asp Abz-Asp-Arg+Gln-Gln-Tyr(NO2)-Asp S - Abz Asp Arg Gln Gln Tyn Asp Than et al., 2004
Abz-Cys-Arg-Gln-Gln-Tyr(NO2)-Asp Abz-Cys-Arg+Gln-Gln-Tyr(NO2)-Asp S - Abz Cys Arg Gln Gln Tyn Asp Than et al., 2004
Abz-Gln-Arg-Gln-Gln-Tyr(NO2)-Asp Abz-Gln-Arg+Gln-Gln-Tyr(NO2)-Asp S - Abz Gln Arg Gln Gln Tyn Asp Than et al., 2004
Abz-Glu-Arg-Gln-Gln-Tyr(NO2)-Asp Abz-Glu-Arg+Gln-Gln-Tyr(NO2)-Asp S - Abz Glu Arg Gln Gln Tyn Asp Than et al., 2004
Abz-Gly-Arg-Gln-Gln-Tyr(NO2)-Asp Abz-Gly-Arg+Gln-Gln-Tyr(NO2)-Asp S - Abz Gly Arg Gln Gln Tyn Asp Than et al., 2004
Abz-His-Arg-Gln-Gln-Tyr(NO2)-Asp Abz-His-Arg+Gln-Gln-Tyr(NO2)-Asp S - Abz His Arg Gln Gln Tyn Asp Than et al., 2004
Abz-Ile-Arg-Gln-Gln-Tyr(NO2)-Asp Abz-Ile-Arg+Gln-Gln-Tyr(NO2)-Asp S - Abz Ile Arg Gln Gln Tyn Asp Than et al., 2004
Abz-Leu-Ala-Gln-Gln-Tyr(NO2)-Asp Abz-Leu-Ala+Gln-Gln-Tyr(NO2)-Asp S - Abz Leu Ala Gln Gln Tyn Asp Than et al., 2004
Abz-Leu-Arg-Gln-Gln-Tyr(NO2)-Asp Abz-Leu-Arg+Gln-Gln-Tyr(NO2)-Asp S - Abz Leu Arg Gln Gln Tyn Asp Than et al., 2004
Abz-Leu-Arg-Gln-Gln-Tyr(NO2)-Asp Abz-Leu-Arg+Gln-Gln-Tyr(NO2)-Asp S - Abz Leu Arg Gln Gln Tyn Asp Than et al., 2004
Abz-Leu-Asn-Gln-Gln-Tyr(NO2)-Asp Abz-Leu-Asn+Gln-Gln-Tyr(NO2)-Asp S - Abz Leu Asn Gln Gln Tyn Asp Than et al., 2004
Abz-Leu-Asp-Gln-Gln-Tyr(NO2)-Asp Abz-Leu-Asp+Gln-Gln-Tyr(NO2)-Asp S - Abz Leu Asp Gln Gln Tyn Asp Than et al., 2004
Abz-Leu-Cys-Gln-Gln-Tyr(NO2)-Asp Abz-Leu-Cys+Gln-Gln-Tyr(NO2)-Asp S - Abz Leu Cys Gln Gln Tyn Asp Than et al., 2004
Abz-Leu-Cys-Gln-Pro-Tyr(NO2)-Asp Abz-Leu-Cys+Gln-Pro-Tyr(NO2)-Asp S - Abz Leu Cys Gln Pro Tyn Asp Davy et al., 2000
Abz-Leu-Gln-Gln-Gln-Tyr(NO2)-Asp Abz-Leu-Gln+Gln-Gln-Tyr(NO2)-Asp S - Abz Leu Gln Gln Gln Tyn Asp Than et al., 2004
Abz-Leu-Glu-Gln-Gln-Tyr(NO2)-Asp Abz-Leu-Glu+Gln-Gln-Tyr(NO2)-Asp S - Abz Leu Glu Gln Gln Tyn Asp Than et al., 2004
Abz-Leu-Gly-Gln-Gln-Tyr(NO2)-Asp Abz-Leu-Gly+Gln-Gln-Tyr(NO2)-Asp S - Abz Leu Gly Gln Gln Tyn Asp Than et al., 2004
Abz-Leu-Gly-Gln-Pro-Tyr(NO2)-Asp Abz-Leu-Gly+Gln-Pro-Tyr(NO2)-Asp S - Abz Leu Gly Gln Pro Tyn Asp Davy et al., 2000
Abz-Leu-His-Gln-Gln-Tyr(NO2)-Asp Abz-Leu-His+Gln-Gln-Tyr(NO2)-Asp S - Abz Leu His Gln Gln Tyn Asp Than et al., 2004
Abz-Leu-Ile-Gln-Gln-Tyr(NO2)-Asp Abz-Leu-Ile+Gln-Gln-Tyr(NO2)-Asp S - Abz Leu Ile Gln Gln Tyn Asp Than et al., 2004
Abz-Leu-Leu-Gln-Gln-Tyr(NO2)-Asp Abz-Leu-Leu+Gln-Gln-Tyr(NO2)-Asp S - Abz Leu Leu Gln Gln Tyn Asp Than et al., 2004
Abz-Leu-Lys-Gln-Gln-Tyr(NO2)-Asp Abz-Leu-Lys+Gln-Gln-Tyr(NO2)-Asp S - Abz Leu Lys Gln Gln Tyn Asp Than et al., 2004
Abz-Leu-Met-Gln-Gln-Tyr(NO2)-Asp Abz-Leu-Met+Gln-Gln-Tyr(NO2)-Asp S - Abz Leu Met Gln Gln Tyn Asp Than et al., 2004
Abz-Leu-Met-Gln-Pro-Tyr(NO2)-Asp Abz-Leu-Met+Gln-Pro-Tyr(NO2)-Asp S - Abz Leu Met Gln Pro Tyn Asp Davy et al., 2000
Abz-Leu-Phe-Gln-Gln-Tyr(NO2)-Asp Abz-Leu-Phe+Gln-Gln-Tyr(NO2)-Asp S - Abz Leu Phe Gln Gln Tyn Asp Than et al., 2004
Abz-Leu-Pro-Gln-Gln-Tyr(NO2)-Asp Abz-Leu-Pro+Gln-Gln-Tyr(NO2)-Asp S - Abz Leu Pro Gln Gln Tyn Asp Than et al., 2004
Abz-Leu-Ser-Gln-Gln-Tyr(NO2)-Asp Abz-Leu-Ser+Gln-Gln-Tyr(NO2)-Asp S - Abz Leu Ser Gln Gln Tyn Asp Than et al., 2004
Abz-Leu-Thr-Gln-Gln-Tyr(NO2)-Asp Abz-Leu-Thr+Gln-Gln-Tyr(NO2)-Asp S - Abz Leu Thr Gln Gln Tyn Asp Than et al., 2004
Abz-Leu-Trp-Gln-Gln-Tyr(NO2)-Asp Abz-Leu-Trp+Gln-Gln-Tyr(NO2)-Asp S - Abz Leu Trp Gln Gln Tyn Asp Than et al., 2004
Abz-Leu-Tyr-Gln-Gln-Tyr(NO2)-Asp Abz-Leu-Tyr+Gln-Gln-Tyr(NO2)-Asp S - Abz Leu Tyr Gln Gln Tyn Asp Than et al., 2004
Abz-Leu-Val-Gln-Gln-Tyr(NO2)-Asp Abz-Leu-Val+Gln-Gln-Tyr(NO2)-Asp S - Abz Leu Val Gln Gln Tyn Asp Than et al., 2004
Abz-Lys-Arg-Gln-Gln-Tyr(NO2)-Asp Abz-Lys-Arg+Gln-Gln-Tyr(NO2)-Asp S - Abz Lys Arg Gln Gln Tyn Asp Than et al., 2004
Abz-Met-Arg-Gln-Gln-Tyr(NO2)-Asp Abz-Met-Arg+Gln-Gln-Tyr(NO2)-Asp S - Abz Met Arg Gln Gln Tyn Asp Than et al., 2004
Abz-Met-Arg-Gln-Gln-Tyr(NO2)-Asp Abz-Met-Arg+Gln-Gln-Tyr(NO2)-Asp S - Abz Met Arg Gln Gln Tyn Asp Davy et al., 2000
Abz-Phe-Arg-Gln-Gln-Tyr(NO2)-Asp Abz-Phe-Arg+Gln-Gln-Tyr(NO2)-Asp S - Abz Phe Arg Gln Gln Tyn Asp Than et al., 2004
Abz-Phe-Arg-Gln-Gln-Tyr(NO2)-Asp Abz-Phe-Arg+Gln-Gln-Tyr(NO2)-Asp S - Abz Phe Arg Gln Gln Tyn Asp Davy et al., 2000
Abz-Ser-Arg-Gln-Gln-Tyr(NO2)-Asp Abz-Ser-Arg+Gln-Gln-Tyr(NO2)-Asp S - Abz Ser Arg Gln Gln Tyn Asp Than et al., 2004
Abz-Thr-Arg-Gln-Gln-Tyr(NO2)-Asp Abz-Thr-Arg+Gln-Gln-Tyr(NO2)-Asp S - Abz Thr Arg Gln Gln Tyn Asp Than et al., 2004
Abz-Trp-Arg-Gln-Gln-Tyr(NO2)-Asp Abz-Trp-Arg+Gln-Gln-Tyr(NO2)-Asp S - Abz Trp Arg Gln Gln Tyn Asp Than et al., 2004
Abz-Tyr-Arg-Gln-Gln-Tyr(NO2)-Asp Abz-Tyr-Arg+Gln-Gln-Tyr(NO2)-Asp S - Abz Tyr Arg Gln Gln Tyn Asp Than et al., 2004
Abz-Tyr-Arg-Gln-Gln-Tyr(NO2)-Asp Abz-Tyr-Arg+Gln-Gln-Tyr(NO2)-Asp S - Abz Tyr Arg Gln Gln Tyn Asp Davy et al., 2000
Abz-Val-Arg-Gln-Gln-Tyr(NO2)-Asp Abz-Val-Arg+Gln-Gln-Tyr(NO2)-Asp S - Abz Val Arg Gln Gln Tyn Asp Than et al., 2004
alpha-1-peptidase inhibitor P01009 25-418 peptide-Met358+Ser-peptide P Ala Ile Pro Met Ser Ile Pro Pro Nelson et al., 1998 19110
alpha-1-peptidase inhibitor P01009 25-418 peptide-Phe376+Leu-peptide N Gly Ala Met Phe Leu Glu Ala Ile Mast et al., 1992
alpha-2-macroglobulin P01023 24-1474 peptide-Arg704+Val-peptide P Glu Gly Leu Arg Val Gly Phe Tyr Mortensen et al., 1981
alpha-2-macroglobulin P01023 24-1474 peptide-Gly706+Phe-peptide P Leu Arg Val Gly Phe Tyr Glu Ser Mortensen et al., 1981
alpha-2-macroglobulin P01023 24-1474 peptide-His722+Val-peptide P Arg Leu Val His Val Glu Glu Pro Mortensen et al., 1981
Antitrypsin P22922 17-392 Peptide-Arg356+Ser-Peptide P NT Thr Met Thr Arg Ser Ser Lys Val Sasaki et al., 1990
Bz-Arg-NHPhNO2 (BAPNA) Bz-Arg+NHPhNO2 S - - Bz Arg NAN - - -
EDANS-EALFAERK-Dabcyl EDANS-Glu-Ala-Leu-Phe+Ala-Glu-Arg-Lys-Dabcyl S Glu Ala Leu Phe Ala Glu Arg Lys Kelly et al., 2009
EDANS-EPAFAERK-Dabcyl EDANS-Glu-Pro-Ala-Phe+Ala-Glu-Arg-Lys-Dabcyl S Glu Pro Ala Phe Ala Glu Arg Lys Kelly et al., 2009
EDANS-EPLAAERK-Dabcyl EDANS-Glu-Pro-Leu-Ala+Ala-Glu-Arg-Lys-Dabcyl S Glu Pro Leu Ala Ala Glu Arg Lys Kelly et al., 2009
EDANS-EPLFAARK-Dabcyl EDANS-Glu-Pro-Leu-Phe+Ala-Ala-Arg-Lys-Dabcyl S Glu Pro Leu Phe Ala Ala Arg Lys Kelly et al., 2009
EDANS-EPLFAEAK-Dabcyl EDANS-Glu-Pro-Leu-Phe+Ala-Glu-Ala-Lys-Dabcyl S Glu Pro Leu Phe Ala Glu Ala Lys Kelly et al., 2009
EDANS-EPLFAERK-Dabcyl EDANS-Glu-Pro-Leu-Phe+Ala-Glu-Arg-Lys-Dabcyl S Glu Pro Leu Phe Ala Glu Arg Lys Kelly et al., 2009
EDANS-EPLFGERK-Dabcyl EDANS-Glu-Pro-Leu-Phe+Gly-Glu-Arg-Lys-Dabcyl S Glu Pro Leu Phe Gly Glu Arg Lys Kelly et al., 2009
EDANS-EPLFMERK-Dabcyl EDANS-Glu-Pro-Leu-Phe+Met-Glu-Arg-Lys-Dabcyl S Glu Pro Leu Phe Met Glu Arg Lys Kelly et al., 2009
EDANS-EPLFMERK-Dabcyl EDANS-Glu-Pro-Leu-Phe-Met+Glu-Arg-Lys-Dabcyl S Pro Leu Phe Met Glu Arg Lys Dab Kelly et al., 2009
Glp-Phe-Ala-NHNap Glp-Phe-Ala+NHNap S - Glp Phe Ala NAP - - - Filippova et al., 2020
Glp-Phe-Gln-NHNap Glp-Phe-Gln+NHNap S - Glp Phe Gln NAP - - - Filippova et al., 2020
Glp-Phe-Leu-NHPhNO2 Glp-Phe-Leu+NHPhNO2 S - Glp Phe Leu NAN - - -
IgG1 heavy chain (hinge region) P01857 1-330 peptide-Thr106+His-peptide P Cys Asp Lys Thr His Thr Cys Pro Gearing et al., 2002 18193
IgG1 heavy chain (hinge region) P01857 1-330 peptide-His6+Thr-peptide P Asp Lys Thr His Thr Cys Pro Pro Ryan et al., 2008 20261
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya+Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N Tyr Leu Val Cya Gly Glu Arg Gly Johansen & Ottesen, 1968
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly+Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N His Leu Cya Gly Ser His Leu Val Johansen & Ottesen, 1968
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu+Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N His Leu Val Glu Ala Leu Tyr Leu Johansen & Ottesen, 1968
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala+Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N Leu Val Glu Ala Leu Tyr Leu Val Johansen & Ottesen, 1968
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu+Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N Val Glu Ala Leu Tyr Leu Val Cya Johansen & Ottesen, 1968
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr+Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N Glu Ala Leu Tyr Leu Val Cya Gly Johansen & Ottesen, 1968
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu+Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N Ala Leu Tyr Leu Val Cya Gly Glu Johansen & Ottesen, 1968
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val+Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N Leu Tyr Leu Val Cya Gly Glu Arg Johansen & Ottesen, 1968
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly+Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N Leu Val Cya Gly Glu Arg Gly Phe Johansen & Ottesen, 1968
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg+Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N Cya Gly Glu Arg Gly Phe Phe Tyr Johansen & Ottesen, 1968
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe+Tyr-Thr-Pro-Lys-Ala N Arg Gly Phe Phe Tyr Thr Pro Lys Johansen & Ottesen, 1968
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr+Thr-Pro-Lys-Ala N Gly Phe Phe Tyr Thr Pro Lys Ala Johansen & Ottesen, 1968
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu+Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N Asn Gln His Leu Cya Gly Ser His Johansen & Ottesen, 1968
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly+Phe-Phe-Tyr-Thr-Pro-Lys-Ala N Gly Glu Arg Gly Phe Phe Tyr Thr Johansen & Ottesen, 1968
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn+Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N - Phe Val Asn Gln His Leu Cya Johansen & Ottesen, 1968
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya+Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N - His Leu Cya Gly Ser His Leu Johansen & Ottesen, 1968
pGlu-Phe-Leu-NHPhNO2 pGlu-Phe-Leu+NHPhNO2 S - Glp Phe Leu NAN - - - Filippova et al., 1984
tetanus toxin P04958 2-457; 458-1315 peptide-Thr446+Asn-peptide N Ile Pro Pro Thr Asn Ile Arg Glu Krieglstein et al., 1991
tetanus toxin P04958 2-457; 458-1315 peptide-Asn447+Ile-peptide N Pro Pro Thr Asn Ile Arg Glu Asn Krieglstein et al., 1991
tetanus toxin P04958 2-457; 458-1315 peptide-Thr460+Asp-peptide N Ala Ser Leu Thr Asp Leu Gly Gly Krieglstein et al., 1991
tetanus toxin P04958 2-457; 458-1315 peptide-Asp461+Leu-peptide N Ser Leu Thr Asp Leu Gly Gly Glu Krieglstein et al., 1991
tetanus toxin P04958 2-457; 458-1315 peptide-Ser864+Lys-peptide N Phe Ser Tyr Ser Lys Asn Leu Asp Krieglstein et al., 1991
Z-Phe-Arg-NHMec Z-Phe-Arg-NHMec S - Z Phe Arg AMC - - - Davy et al., 1998

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