Literature for peptidase C01.001: papain

Summary Alignment Sequences Sequence features Distribution Structure Literature Substrates Pharma

(Topics flags: A Assay, S Structure, P Specificity, I Inhibitor, E Expression, U Therapeutic, V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

2025

Ansari,M., Prajapati,K.P., Mittal,S., Mishra,S., Gupta,V., Anand,B.G. and Kar,K. High-low temperature switch induces formation of catalytic and cytotoxic amyloid aggregates of papain Food Chem (2025) 479, 143657-143657. PubMed Europe PubMed DOI
Choudhary,R., Kaushik,R., Chawla,P. and Manna,S. Exploring the extraction, functional properties, and industrial applications of papain from Carica papaya J Sci Food Agric (2025) 105, 1533-1545. PubMed Europe PubMed DOI V
Juliano,M.A., Persico,M., Severino,B., Tumbarello,G., Okamoto,D., Fernandes,K.R., Trigo,G., Tanaka,A.S., Lacerda,J.T., Tkachuck,O., Corvino,A., Fiorino,F., Scognamiglio,A., Frecentese,F., Santagada,V., Vertuccio,S., Caliendo,G., Juliano,L. and Fattorusso,C. 1,2,4-Thiadiazolidin-3,5-Diones as Inhibitors of Cysteine Proteases Molecules (2025) 30 PubMed Europe PubMed DOI PMC EPMC I
Vlahakis,N.W., Flowers,C.W., Liu,M., Agdanowski,M.P., Johnson,S., Summers,J.A., Jacobs,L.M.C., Keyser,C., Russell,P., Rose,S.L., Orlans,J., Adhami,N., Chen,Y., Sawaya,M.R., Basu,S., de Sanctis,D., Chen,Y., Wakatsuki,S., Nelson,H.M., Loo,J.A., Tang,Y. and Rodriguez,J.A. Combining MicroED and native mass spectrometry for structural discovery of enzyme-small molecule complexes Proc Natl Acad Sci U S A (2025) 122, e2503780122-e2503780122. PubMed Europe PubMed DOI PMC EPMC I

2024

Glahn,J.Z., Wang-Evers,M., Carlson,A.R., Marks,H., Karasik,D., Hilge,F., Goverman,J. and Manstein,D. Evaluation of Papain-Urea for Enzymatic Debridement of Coagulation Zones Following Ablative Fractional Laser Treatment Lasers Surg Med (2024) PubMed Europe PubMed DOI

2023

Liu,X., Huang,Q., Pan,P., Fang,M., Zhang,Y., Yang,S., Li,M. and Liu,Y. Comparative Study of the Preparation of High-Molecular-Weight Fibroin by Degumming Silk with Several Neutral Proteases Polymers (Basel) (2023) 15 PubMed Europe PubMed DOI PMC EPMC

2022

Bruce,B. Enzyme treatment of red blood cells: use of ficin and papain Immunohematology (2022) 38, 90-95. PubMed Europe PubMed DOI V

2021

Annahazi,A., Schroder,A. and Schemann,M. Region-specific effects of the cysteine protease papain on gastric motility Neurogastroenterol Motil (2021) , e14105-e14105. PubMed Europe PubMed DOI
Baidamshina,D.R., Koroleva,V.A., Olshannikova,S.S., Trizna,E.Y., Bogachev,M.I., Artyukhov,V.G., Holyavka,M.G. and Kayumov,A.R. Biochemical Properties and Anti-Biofilm Activity of Chitosan-Immobilized Papain Mar Drugs (2021) 19 PubMed Europe PubMed DOI PMC EPMC
Kunimine,S., Takai,T., Kamijo,S., Maruyama,N., Kimitsu,T., Masutani,Y., Yoshimura,T., Suchiva,P., Shimizu,S., Ogawa,H., Okumura,K. and Ikeda,S. Epicutaneous vaccination with protease inhibitor-treated papain prevents papain-induced Th2-mediated airway inflammation without inducing Th17 in mice Biochem Biophys Res Commun (2021) 546, 192-199. PubMed Europe PubMed DOI

2020

Azarkan,M., Maquoi,E., Delbrassine,F., Herman,R., M'rabet,N., Calvo Esposito,R., Charlier,P. and Kerff,F. Structures of the free and inhibitors-bound forms of bromelain and ananain from Ananas comosus stem and in vitro study of their cytotoxicity Sci Rep (2020) 10, 19570-19570. PubMed Europe PubMed DOI PMC EPMC I
Feng,N., Zhang,H., Li,Y., Liu,Y., Xu,L., Wang,Y., Fei,X. and Tian,J. A novel catalytic material for hydrolyzing cow's milk allergenic proteins: papain-Cu3(PO4)2.3H2O-magnetic nanoflowers Food Chem (2020) 311, 125911-125911. PubMed Europe PubMed DOI
Filippova,I.Y., Dvoryakova,E.A., Sokolenko,N.I., Simonyan,T.R., Tereshchenkova,V.F., Zhiganov,N.I., Dunaevsky,Y.E., Belozersky,M.A., Oppert,B. and Elpidina,E.N. New Glutamine-Containing Substrates for the Assay of Cysteine Peptidases From the C1 Papain Family Front Mol Biosci (2020) 7, 578758-578758. PubMed Europe PubMed DOI PMC EPMC
Fukuda,K., Matsuzaki,H., Mikami,Y., Makita,K., Miyakawa,K., Miyashita,N., Hosoki,K., Ishii,T., Noguchi,S., Urushiyama,H., Horie,M., Mitani,A., Yamauchi,Y., Shimura,E., Nakae,S., Saito,A., Nagase,T. and Hiraishi,Y. A mouse model of asthma-chronic obstructive pulmonary disease overlap induced by intratracheal papain Allergy (2020) PubMed Europe PubMed DOI
Hafid,K., John,J., Sayah,T.M., Dominguez,R., Becila,S., Lamri,M., Dib,A.L., Lorenzo,J.M. and Gagaoua,M. One-step recovery of latex papain from Carica papaya using Three Phase Partitioning and its use as milk-clotting and meat-tenderizing agent Int J Biol Macromol (2020) 146, 798-810. PubMed Europe PubMed DOI
Jastaniah,A., Gaisina,I.N., Knopp,R.C. and Thatcher,G.R.J. Synthesis of alpha-Ketoamide-Based Stereoselective Calpain-1 Inhibitors as Neuroprotective Agents ChemMedChem (2020) PubMed Europe PubMed DOI I
Lima,A.M., Barros,N.L.F., Freitas,A.C.O., Tavares,L.S.C., Pirovani,C.P., Siqueira,A.S., Goncalves,E.C. and de Souza,C.R.B. A new Piper nigrum cysteine proteinase inhibitor, PnCPI, with antifungal activity: molecular cloning, recombinant expression, functional analyses and molecular modeling Planta (2020) 252, 16-16. PubMed Europe PubMed DOI
Lin,K.P., Feng,G.J., Pu,F.L., Hou,X.D. and Cao,S.L. Enhancing the Thermostability of Papain by Immobilizing on Deep Eutectic Solvents-Treated Chitosan With Optimal Microporous Structure and Catalytic Microenvironment Front Bioeng Biotechnol (2020) 8, 576266-576266. PubMed Europe PubMed DOI PMC EPMC
Meenu,S., Pradeep,B., Ramalingam,S., Sairam,T., Rai,R. and Sankaran,R. Papillon-Lefevre syndrome (PLS) with novel compound heterozygous mutation in the exclusion and Peptidase C1A domains of Cathepsin C gene Mol Biol Rep (2020) PubMed Europe PubMed DOI
Mishra,M., Singh,V., Tellis,M.B., Joshi,R.S. and Singh,S. Repurposing the McoTI-II Rigid Molecular Scaffold in to Inhibitor of 'Papain Superfamily' Cysteine Proteases Pharmaceuticals (Basel) (2020) 14 PubMed Europe PubMed DOI PMC EPMC
Moreira Filho,R.N.F., Vasconcelos,N.F., Andrade,F.K., Rosa,M.F. and Vieira,R.S. Papain immobilized on alginate membrane for wound dressing application Colloids Surf B Biointerfaces (2020) 194, 111222-111222. PubMed Europe PubMed DOI
Shinu,P., Singh,V.A., Nair,A., Venugopala,K.N. and Akrawi,S.H. Papain-cetylpyridinium chloride and pepsin-cetylpyridinium chloride; two novel, highly sensitive, concentration, digestion and decontamination techniques for culturing mycobacteria from clinically suspected pulmonary tuberculosis cases PLoS ONE (2020) 15, e0236700-e0236700. PubMed Europe PubMed DOI PMC EPMC
Soares,A.M.B.F., Goncalves,L.M.O., Ferreira,R.D.S., de Souza,J.M., Fangueiro,R., Alves,M.M.M., Carvalho,F.A.A., Mendes,A.N. and Cantanhede,W. Immobilization of papain enzyme on a hybrid support containing zinc oxide nanoparticles and chitosan for clinical applications Carbohydr Polym (2020) 243, 116498-116498. PubMed Europe PubMed DOI
Wang,X., Gao,Y., Guan,Z., Xie,Z., Zhang,D., Yin,P., Yang,G., Hong,D. and Xin,Q. Structural analysis of the meiosis-related protein MS5 reveals non-canonical papain enhancement by cystatin-like folds FEBS Lett (2020) 594, 2462-2471. PubMed Europe PubMed DOI S

2019

Gimenez-Dejoz,J., Tsuchiya,K. and Numata,K. Insights into the stereospecificity in papain-mediated chemoenzymatic polymerization from quantum mechanics/molecular mechanics simulations ACS Chem Biol (2019) 14, 1280-1292. PubMed Europe PubMed DOI
Liu,C., Barrett,T.M., Chen,X., Ferrie,J.J. and Petersson,E.J. Fluorescent probes for studying thioamide positional effects on proteolysis reveal insight into resistance to cysteine proteases Chembiochem (2019) 20, 2059-2062. PubMed Europe PubMed DOI I
Milosevic,J., Jankovic,B., Prodanovic,R. and Polovic,N. Comparative stability of ficin and papain in acidic conditions and the presence of ethanol Amino Acids (2019) 51, 829-838. PubMed Europe PubMed DOI
Mohan,V., Das,N., Das,A., Mishra,V. and Sen,P. Spectroscopic insight on ethanol-induced aggregation of papain J Phys Chem B (2019) 123, 2280-2290. PubMed Europe PubMed DOI
Sang,M., Xu,C., Wei,Z., Wu,X., Guo,Y., Li,J., Wang,Z. and Zhang,J. Cloning and high-level SUMO-mediated fusion expression of a serine protease inhibitor from Hyphantria cunea Drury that exhibits activity against papain Protein Expr Purif (2019) 158, 36-43. PubMed Europe PubMed DOI
Santis,L.P., Garcia,P.C., Secco,V.N.D.P., Ferreira,R.R. and Deffune,E. Applicability of papain solutions in immunohematology Einstein (Sao Paulo) (2019) 17, eAO4328-eAO4328. PubMed Europe PubMed DOI PMC EPMC
Yu,L., Zhang,H., Yang,L. and Tian,K. Optimization of purification conditions for papain in a polyethylene glycol-phosphate aqueous two-phase system using quaternary ammonium ionic liquids as adjuvants by BBD-RSM Protein Expr Purif (2019) 156, 8-16. PubMed Europe PubMed DOI

2018

Gu,X., Gao,J., Li,X. and Wang,Y. Immobilization of papain onto graphene oxide nanosheets J Nanosci Nanotechnol (2018) 18, 3543-3547. PubMed Europe PubMed DOI
Gu,Y.J., Zhu,M.L., Li,Y.L. and Xiong,C.H. Research of a new metal chelating carrier preparation and papain immobilization Int J Biol Macromol (2018) 112, 1175-1182. PubMed Europe PubMed DOI

2017

Lin,M., Yu,T., Wan,J. and Cao,X. Prediction of the reverse micellar extraction of papain using dissipative particle dynamics simulation Appl Biochem Biotechnol (2017) 181, 1338-1346. PubMed Europe PubMed DOI
Moraes,D., Levenhagen,M.A., Costa-Cruz,J.M., Costa AP Netto and Rodrigues,R.M. In vitro efficacy of latex and purified papain from Carica papaya against Strongyloides venezuelensis eggs and larvae Rev Inst Med Trop Sao Paulo (2017) 59, e7-e7. PubMed Europe PubMed DOI
Moraes,D., Levenhagen,M.A., Costa-Cruz,J.M., daCosta-Netto,A.P. and Rodrigues,R.M. In vitro efficacy of latex and purified papain from Carica papaya against Strongyloides venezuelensis eggs and larvae Rev Inst Med Trop Sao Paulo (2017) 59 DOI
Mugita,N., Nambu,T., Takahashi,K., Wang,P.L. and Komasa,Y. Proteases, actinidin, papain and trypsin reduce oral biofilm on the tongue in elderly subjects and in vitro Arch Oral Biol (2017) 82, 233-240. PubMed Europe PubMed DOI U
Prabhu A,A., Chityala,S., Garg,Y. and Venkata Dasu,V. Reverse micellar extraction of papain with cationic detergent based system: an optimization approach Prep Biochem Biotechnol (2017) 47, 236-244. PubMed Europe PubMed DOI

2016

Dutta,S., Choudhury,D., Roy,S., Dattagupta,J.K. and Biswas,S. Mutation in the pro-peptide region of a cysteine protease leads to altered activity and specificity - a structural and biochemical approach PLoS ONE (2016) 11, e0158024-e0158024. PubMed Europe PubMed DOI
Fekete,A. and Komaromi,I. Modeling the archetype cysteine protease reaction using dispersion corrected density functional methods in ONIOM-type hybrid QM/MM calculations; the proteolytic reaction of papain Phys Chem Chem Phys (2016) 18, 32847-32861. PubMed Europe PubMed DOI
Li,Q., Zhou,Y., Ma,K., Wei,Q. and Nie,Z. A mesoporous SiO2/dense SiO2/Fe3O4 multiply coated hollow microsphere: synthesis and application on papain immobilization Colloids Surf A Physicochem Eng Asp (2016) 511, 239-246. DOI
Maria Manohar,C. and Doble,M. Papain immobilized polyurethane as an ureteral stent material J Biomed Mater Res B Appl Biomater (2016) 104, 723-731. PubMed Europe PubMed DOI
Muller,A., Barat,S., Chen,X., Bui,K.C., Bozko,P., Malek,N.P. and Plentz,R.R. Comparative study of antitumor effects of bromelain and papain in human cholangiocarcinoma cell lines Int J Oncol (2016) 48, 2025-2034. PubMed Europe PubMed DOI U
Rocha,M.V., DiGiacomo,M., Beltramino,S., Loh,W., Romanini,D. and Nerli,B.B. A sustainable affinity partitioning process to recover papain from Carica papaya latex using alginate as macro-ligand Sep Purif Technol (2016) 168, 168-176. DOI
Tsuchiya,K. and Numata,K. Papain-catalyzed chemoenzymatic synthesis of telechelic polypeptides using bis(leucine ethyl ester) initiator Macromol Biosci (2016) 16, 1001-1008. PubMed Europe PubMed DOI
Yu,J., Lin,F., Lin,S., Pei,X., Miao,J., Chen,X., Wu,S.G. and Wang,A. A comparative study of papain and bromelain in enzymatic oligomerization of l-Phe methyl ester in aqueous environment J Mol Catal B Enzym (2016) 133, S95-S99. DOI

2015

Benucci,I., Esti,M. and Liburdi,K. Effect of wine inhibitors on the proteolytic activity of papain from Carica papaya L. latex Biotechnol Prog (2015) 31, 48-54. PubMed Europe PubMed DOI I
Homaei,A. Enhanced activity and stability of papain immobilized on CNBr-activated sepharose Int J Biol Macromol (2015) 75, 373-377. PubMed Europe PubMed DOI
Jiang,Z.G., Zhang,H.D. and Wang,W.T. Purification of papain by metal affinity partitioning in aqueous two-phase polyethylene glycol/sodium sulfate systems J Sep Sci (2015) 38, 1426-1432. PubMed Europe PubMed DOI
Junior,Z.S., Botta,S.B., Ana,P.A., Franca,C.M., Fernandes,K.P., Mesquita-Ferrari,R.A., Deana,A. and Bussadori,S.K. Effect of papain-based gel on type I collagen - spectroscopy applied for microstructural analysis Sci Rep (2015) 5, 11448-11448. PubMed Europe PubMed DOI PMC EPMC U
Mera,T. and Faustman,D.L. Removal of donor human leukocyte antigen class I proteins with papain: translation for possible whole organ practices Transplantation (2015) 99, 724-730. PubMed Europe PubMed DOI
Mitsuhashi,J., Nakayama,T. and Narai-Kanayama,A. Mechanism of papain-catalyzed synthesis of oligo-tyrosine peptides Enzyme Microb Technol (2015) 75-76, 10-17. PubMed Europe PubMed DOI
Nishikado,H., Fujimura,T., Taka,H., Mineki,R., Ogawa,H., Okumura,K. and Takai,T. Cysteine protease antigens cleave CD123, the alpha subunit of murine IL-3 receptor, on basophils and suppress IL-3-mediated basophil expansion Biochem Biophys Res Commun (2015) 460, 261-266. PubMed Europe PubMed DOI
Raskovic,B., Popovic,M., Ostojic,S., Andelkovic,B., Tesevic,V. and Polovic,N. Fourier transform infrared spectroscopy provides an evidence of papain denaturation and aggregation during cold storage Spectrochim Acta A Mol Biomol Spectrosc (2015) 150, 238-246. PubMed Europe PubMed DOI
Ribeiro,A.P., Oliveira,B.G., Soares,M.F., Barreto,B.M., Futuro,D.O. and Castilho,S.R. [Effectiveness of 2% and 4% papain gels in the healing of venous ulcers] Rev Esc Enferm USP (2015) 49, 394-402. PubMed Europe PubMed DOI U
Rodrigues,A.L., Oliveira,B.G., Futuro,D.O. and Secoli,S.R. Effectiveness of papain gel in venous ulcer treatment: randomized clinical trial Rev Lat Am Enfermagem (2015) 23, 458-465. PubMed Europe PubMed DOI U
Wang,W., Jiang,Z., Zhang,H., Peng,J., Xu,Y., Dong,A., Yang,X. and Jiang,X. Partitioning behavior and structural characterization of papain in ionic liquid aqueous two-phase system Huagong Xuebao (2015) 66, 179-185. DOI

2014

Dong,A.-H., Peng,J., Xu,Y.-H., Wang,W.-T., Jiang,X.-X. and Zhang,H.-D. Correlation of PEG/(NH4)2SO4 aqueous two-phase equilibrium data and modeling of partition of papain Mod Food Sci Technol (2014) 30, 194-199. DOI
Doolittle,R.F. Clotting of mammalian fibrinogens by papain: a re-examination Biochemistry (2014) 53, 6687-6694. PubMed Europe PubMed DOI
Kathman,S.G., Xu,Z. and Statsyuk,A.V. A fragment-based method to discover irreversible covalent inhibitors of cysteine proteases J Med Chem (2014) 57, 4969-4974. PubMed Europe PubMed DOI I
Le,Z.G., Ni,K., Guo,L.T. and Xie,Z.B. The condensation reaction of 4-nitrobenzaldehyde and rhodanine catalyzed by papain Adv Mater Res (2014) 830, 111-114. DOI
Miyazawa,T., Horimoto,T. and Tanaka,K. Kinetically controlled peptide synthesis mediated by papain using the carbamoylmethyl ester as an acyl donor Int J Pept Res Ther (2014) 20, 371-376. DOI
Muller,C., Perera,G., Konig,V. and Bernkop-Schnurch,A. Development and in vivo evaluation of papain-functionalized nanoparticles Eur J Pharm Biopharm (2014) 87, 125-131. PubMed Europe PubMed DOI U
Rosenstein,R.K., Bezbradica,J.S., Yu,S. and Medzhitov,R. Signaling pathways activated by a protease allergen in basophils Proc Natl Acad Sci U S A (2014) 111, E4963-E4971. PubMed Europe PubMed DOI
Wammes,A.E., Hendriks,T.G., Amatdjais-Groenen,H.I., Wijdeven,M.A., van Hest,J.C., van Delft,F.L., Ritschel,T. and Rutjes,F.P. Influence of azide incorporation on binding affinity by small papain inhibitors Bioorg Med Chem (2014) 22, 5593-5603. PubMed Europe PubMed DOI I
Wang,W.-T., Zhang,H.-D., Jiang,Z.-G., Xu,Y.-H., Dong,A.-H., Peng,J. and Yang,X.-F. Extraction of papain by ionic liquid/aqueous two-phase system and optimization of process conditions Mod Food Sci Technol (2014) 30, 210-216. DOI

2013

Braia,M., Ferrero,M., Rocha,M.V., Loureiro,D., Tubio,G. and Romanini,D. Bioseparation of papain from Carica papaya latex by precipitation of papain-poly (vinyl sulfonate) complexes Protein Expr Purif (2013) 91, 91-95. PubMed Europe PubMed DOI
Esti,M., Benucci,I., Lombardelli,C., Liburdi,K. and Garzillo,A.M.V. Papain from papaya (Carica papaya L.) fruit and latex: preliminary characterization in alcoholic-acidic buffer for wine application Food Bioprod Process (2013) 91, 595-598. DOI
Kamijo,S., Takeda,H., Tokura,T., Suzuki,M., Inui,K., Hara,M., Matsuda,H., Matsuda,A., Oboki,K., Ohno,T., Saito,H., Nakae,S., Sudo,K., Suto,H., Ichikawa,S., Ogawa,H., Okumura,K. and Takai,T. IL-33-Mediated innate response and adaptive immune cells contribute to maximum responses of protease allergen-induced allergic airway inflammation J Immunol (2013) 190, 4489-4499. PubMed Europe PubMed DOI
Rathnasamy,S. and Kumaresan,R. Design and development of single stage purification of papain using ionic liquid based aqueous two phase extraction system and its partition coefficient studies Intern J Eng Technol (2013) 5, 1934-1941.
Rocha,M.V. and Nerli,B.B. Molecular features determining different partitioning patterns of papain and bromelain in aqueous two-phase systems Int J Biol Macromol (2013) 61, 204-211. PubMed Europe PubMed DOI
Wei,D., Huang,X., Liu,J., Tang,M. and Zhan,C.G. Reaction pathway and free energy profile for papain-catalyzed hydrolysis of N-acetyl-Phe-Gly 4-nitroanilide Biochemistry (2013) 52, 5145-5154. PubMed Europe PubMed DOI

2012

Bhardwaj,A. and Bhardwaj,S.V. Papacarie(R) containing papain: a natural chemomechanical caries removal agent Res J Pharm Biol Chem Sci (2012) 3, 660-666. U
Campbell,R.L. and Davies,P.L. Structure-function relationships in calpains Biochem J (2012) 447, 335-351. PubMed Europe PubMed DOI V
Chen,J.S., Tian,J.C., Deng,Z.Y., Zhang,Y.X., Feng,S.L., Yan,Z.C., Zhang,X.Y. and Yuan,H.Q. Effects of papain hydrolysis on the pasting properties of wheat flour J Integr Agric (2012) 11, 1948-1957. DOI
Hu,W., Guan,Z., Deng,X. and He,Y.H. Enzyme catalytic promiscuity: the papain-catalyzed Knoevenagel reaction Biochimie (2012) 94, 656-661. PubMed Europe PubMed DOI
Kim,C.J., Lee,D.I., Lee,C.H. and Ahn,I.S. A dityrosine-based substrate for a protease assay: application for the selective assessment of papain and chymopapain activity Anal Chim Acta (2012) 723, 101-107. PubMed Europe PubMed DOI A
Leite,A.P., de Oliveira,B.G., Soares,M.F. and Barrocas,D.L. [Use and effectiveness of papain in the wound healing process: a systematic review] Rev Gaucha Enferm (2012) 33, 198-207. PubMed Europe PubMed U
Llerena-Suster,C.R., Jose,C., Collins,S.E., Briand,L.E. and Morcelle,S.R. Investigation of the structure and proteolytic activity of papain in aqueous miscible organic media Process Biochem (2012) 47, 47-56. DOI
Nishiyama,K. Local fluctuation control of papain by changing a highly fluctuating residue Chem Phys Lett (2012) 555, 226-229. DOI
Pithon,M.M., de Souza Ferraz,C., do Couto de Oliveira,G., Pereira,T.B., Oliveira,D.D., de Souza,R.A., de Freitas,L.M. and Dos Santos,R.L. Effect of 10% papain gel on enamel deproteinization before bonding procedure Angle Orthod (2012) 82, 541-545. PubMed Europe PubMed DOI U
Roy,S., Choudhury,D., Aich,P., Dattagupta,J.K. and Biswas,S. The structure of a thermostable mutant of pro-papain reveals its activation mechanism Acta Crystallogr D Biol Crystallogr (2012) 68, 1591-1603. PubMed Europe PubMed DOI
Storer,A.C. and Menard,R. Papain [ISSN:978-0-12-407743-0] (2012) 3, 1858-1861. DOI

2011

de Beer,R.J., Zarzycka,B., Amatdjais-Groenen,H.I., Jans,S.C., Nuijens,T., Quaedflieg,P.J., van Delft,F.L., Nabuurs,S.B. and Rutjes,F.P. Papain-catalyzed peptide bond formation: enzyme-specific activation with guanidinophenyl esters Chembiochem (2011) 12, 2201-2207. PubMed Europe PubMed DOI
Fosado-Quiroz,R.E. and Rojo-Dominguez,A. Metastability of papain and the molecular mechanism for its sequential acid-denaturation Protein J (2011) 30, 184-193. PubMed Europe PubMed DOI
Hussain,S., Khan,A., Gul,S., Resmini,M., Verma,C.S., Thomas,E.W. and Brocklehurst,K. Identification of interactions involved in the generation of nucleophilic reactivity and of catalytic competence in the catalytic site Cys/His ion pair of papain Biochemistry (2011) 50, 10732-10742. PubMed Europe PubMed DOI
Llerena-Suster,C.R., Priolo,N.S. and Morcelle,S.R. Sodium tetrathionate effect on papain purification from different Carica papaya latex crude extracts Prep Biochem Biotechnol (2011) 41, 107-121. PubMed Europe PubMed DOI
Nishiyama,K. Substrate specificity of papain dynamic structures for peptides consisting of 8-10 Gly residues Chem Phys Lett (2011) 501, 513-516. DOI I
Shokhen,M., Khazanov,N. and Albeck,A. The mechanism of papain inhibition by peptidyl aldehydes Proteins (2011) 79, 975-985. PubMed Europe PubMed DOI I
Wang,G., Xi,H., Tian,F., Han,M. and Lu,Y. Mechanism of molecular interaction between ethionamide and papain: spectroscopic and molecular simulation investigations Acta Chim Sin (2011) 69, 95-100.
Wang,M., Qi,W., Yu,Q., Su,R. and He,Z. Kinetically controlled enzymatic synthesis of dipeptide precursor of l-alanyl-l-glutamine Biotechnol Appl Biochem (2011) 58, 449-455. PubMed Europe PubMed DOI

2010

Ajlia,S.A.S.H., Majid,F.A.A., Suvik,A., Effendy,M.A.W. and Serati Nouri,H. Efficacy of papain-based wound cleanser in promoting wound regeneration Pak J Biol Sci (2010) 13, 596-603. PubMed Europe PubMed U
Choudhury,D., Biswas,S., Roy,S. and Dattagupta,J.K. Improving thermostability of papain through structure-based protein engineering Protein Eng Des Sel (2010) 23, 457-467. PubMed Europe PubMed DOI E
da Silva,C.R., Oliveira,M.B., Motta,E.S., de Almeida,G.S., Varanda,L.L., de Padula,M., Leitao,A.C. and Caldeira-de-Araujo,A. Genotoxic and cytotoxic safety evaluation of papain (Carica papaya L.) using in vitro assays J Biomed Biotechnol (2010) 2010, 197898-197898. PubMed Europe PubMed DOI PMC EPMC U
Jeong,J. and Hur,W. Even-numbered peptides from a papain hydrolysate of silk fibroin J Chromatogr B Analyt Technol Biomed Life Sci (2010) 878, 836-840. PubMed Europe PubMed DOI P
Li,M., Su,E., You,P., Gong,X., Sun,M., Xu,D. and Wei,D. Purification and in situ immobilization of papain with aqueous two-phase system PLoS ONE (2010) 5, e15168-e15168. PubMed Europe PubMed DOI PMC EPMC
Narai-Kanayama,A., Shikata,Y., Hosono,M. and Aso,K. High level production of bioactive di- and tri-tyrosine peptides by protease-catalyzed reactions J Biotechnol (2010) 150, 343-347. PubMed Europe PubMed DOI
Reddy,V.B. and Lerner,E.A. Plant cysteine proteases that evoke itch activate protease-activated receptors Br J Dermatol (2010) 163, 532-535. PubMed Europe PubMed DOI
Singh,J.P., Tamang,S., Rajamohanan,P.R., Jima,N.C., Chakraborty,G., Kundu,G.C., Gaikwad,S.M. and Khan,M.I. Isolation, structure, and functional elucidation of a modified pentapeptide, cysteine protease inhibitor (CPI-2081) from Streptomyces species 2081 that exhibit inhibitory effect on cancer cell migration J Med Chem (2010) 53, 5121-5128. PubMed Europe PubMed DOI I

2009

Bertassoni,L.E. and Marshall,G.W. Papain-gel degrades intact nonmineralized type I collagen fibrils Scanning (2009) 31, 253-258. PubMed Europe PubMed DOI U
Chen,T.X., Nie,H.L., Li,S.B., Branford-White,C., Su,S.N. and Zhu,L.M. Comparison: adsorption of papain using immobilized dye ligands on affinity membranes Colloids Surf B Biointerfaces (2009) 72, 25-31. PubMed Europe PubMed DOI
Choudhury,D., Roy,S., Chakrabarti,C., Biswas,S. and Dattagupta,J.K. Production and recovery of recombinant propapain with high yield Phytochemistry (2009) 70, 465-472. PubMed Europe PubMed DOI E
Sathish,H.A., Kumar,P.R. and Prakash,V. The differential stability of the left and right domains of papain Process Biochem (2009) 44, 710-716. DOI
Shokhen,M., Khazanov,N. and Albeck,A. Challenging a paradigm: theoretical calculations of the protonation state of the Cys25-His159 catalytic diad in free papain Proteins (2009) 77, 916-926. PubMed Europe PubMed DOI PMC EPMC

2008

Beavers,M.P., Myers,M.C., Shah,P.P., Purvis,J.E., Diamond,S.L., Cooperman,B.S., Huryn,D.M. and Smith,A.B., III Molecular docking of cathepsin L inhibitors in the binding site of papain J Chem Inf Model (2008) 48, 1464-1472. PubMed Europe PubMed DOI S I
Gul,S., Hussain,S., Thomas,M.P., Resmini,M., Verma,C.S., Thomas,E.W. and Brocklehurst,K. Generation of nucleophilic character in the Cys25/His159 ion pair of papain involves Trp177 but not Asp158 Biochemistry (2008) 47, 2025-2035. PubMed Europe PubMed DOI
Narai-Kanayama,A., Koshino,H. and Aso,K. Mass spectrometric and kinetic studies on slow progression of papain-catalyzed polymerization of l-glutamic acid diethyl ester Biochim Biophys Acta (2008) 1780, 881-891. PubMed Europe PubMed DOI

2007

Brouwer,A.J., Bunschoten,A. and Liskamp,R.M. Synthesis and evaluation of chloromethyl sulfoxides as a new class of selective irreversible cysteine protease inhibitors Bioorg Med Chem (2007) 15, 6985-6993. PubMed Europe PubMed DOI I
Fatima,S. and Khan,R.H. Effect of polyethylene glycols on the function and structure of thiol proteases J Biochem (2007) 142, 65-72. PubMed Europe PubMed DOI
Sathish,H.A., Kumar,P.R. and Prakash,V. Mechanism of solvent induced thermal stabilization of papain Int J Biol Macromol (2007) 41, 383-390. PubMed Europe PubMed DOI
Theodorou,L.G., Bieth,J.G. and Papamichael,E.M. The catalytic mode of cysteine proteinases of papain (C1) family Bioresour Technol (2007) 98, 1931-1939. PubMed Europe PubMed DOI
Yamamoto,N., Nakayama,A., Oshima,M., Kawasaki,N. and Aiba,S. Enzymatic hydrolysis of lysine diisocyanate based polyurethanes and segmented polyurethane ureas by various proteases React Funct Polym (2007) 67, 1338-1345. DOI

2006

[YEAR:1-6-2006]Alphey,M.S. and Hunter,W.N. High-resolution complex of papain with remnants of a cysteine protease inhibitor derived from Trypanosoma brucei Acta Crystallograph Sect F Struct Biol Cryst Commun (1-6-2006) 62, 504-508. PubMed Europe PubMed DOI PMC EPMC S
[YEAR:6-3-2006]Choe,Y., Leonetti,F., Greenbaum,D.C., Lecaille,F., Bogyo,M., Bromme,D., Ellman,J.A. and Craik,C.S. Substrate profiling of cysteine proteases using a combinatorial peptide library identifies functionally unique specificities J Biol Chem (6-3-2006) 281, 12824-12832. PubMed Europe PubMed DOI P
Geng,L., Vaidya,A., Viswanathan,K., Junru,C., Wenchun,X., Wei,G. and Gross,R.A. Rapid regioselective oligomerization of L-glutamic acid diethyl ester catalyzed by papain Macromolecules (2006) 39, 7915-7921. DOI
Lou,W.Y., Zong,M.H., Smith,T.J., Wu,H. and Wang,J.F. Impact of ionic liquids on papain: an investigation of structure-function relationships Green Chem (2006) 8, 509-512. DOI
Naeem,A., Fatima,S. and Khan,R.H. Characterization of partially folded intermediates of papain in presence of cationic, anionic, and nonionic detergents at low pH Biopolymers (2006) 83, 1-10. PubMed Europe PubMed DOI
Nitsawang,S., Hatti-Kaul,R. and Kanasawud,P. Purification of papain from Carica papaya latex: aqueous two-phase extraction versus two-step salt precipitation Enzyme Microb Technol (2006) 39, 1103-1107. DOI

2005

Afshar,R.K., Patra,A.K. and Mascharak,P.K. Light-induced inhibition of papain by a {Mn-NO}6 nitrosyl: identification of papain-SNO adduct by mass spectrometry J Inorg Biochem (2005) 99, 1458-1464. PubMed Europe PubMed DOI
[YEAR:2-5-2005]Lepp,Z. and Chuman,H. Connecting traditional QSAR and molecular simulations of papain hydrolysis - importance of charge transfer Bioorg Med Chem (2-5-2005) 13, 3093-3105. PubMed Europe PubMed DOI
Roy,J.J., Sumi,S., Sangeetha,K. and Abraham,T.E. Chemical modification and immobilization of papain J Chem Technol Biotechnol (2005) 80, 184-188.

2004

Janowski,R., Kozak,M., Jankowska,E., Grzonka,Z. and Jaskolski,M. Two polymorphs of a covalent complex between papain and a diazomethylketone inhibitor J Pept Res (2004) 64, 141-150. PubMed Europe PubMed DOI S I
Konno,K., Hirayama,C., Nakamura,M., Tateishi,K., Tamura,Y., Hattori,M. and Kohno,K. Papain protects papaya trees from herbivorous insects: role of cysteine proteases in latex Plant J (2004) 37, 370-378. PubMed Europe PubMed DOI
Menard,R. and Storer,A.C. Papain [ISSN:0-12-079610-4] (2004) 2, 1125-1128. V

2003

[YEAR:25-6-2003]Azarkan,M., El Moussaoui,A., van Wuytswinkel,D., Dehon,G. and Looze,Y. Fractionation and purification of the enzymes stored in the latex of Carica papaya J Chromatogr B Analyt Technol Biomed Life Sci (25-6-2003) 790, 229-238. PubMed Europe PubMed DOI
[YEAR:1-8-2003]Dardenne,L.E., Werneck,A.S., de Oliveira Neto,M. and Bisch,P.M. Electrostatic properties in the catalytic site of papain: a possible regulatory mechanism for the reactivity of the ion pair Proteins (1-8-2003) 52, 236-253. PubMed Europe PubMed DOI

2002

[YEAR:6-11-2002]Reddy,S.Y., Kahn,K., Zheng,Y.J. and Bruice,T.C. Protein engineering of nitrile hydratase activity of papain: molecular dynamics study of a mutant and wild-type enzyme J Am Chem Soc (6-11-2002) 124, 12979-12990. PubMed Europe PubMed DOI
Versari,A., Menard,R. and Lortie,R. Enzymatic hydrolysis of nitriles by an engineered nitrile hydratase (papain Gln19Glu) in aqueous-organic media Biotechnol Bioeng (2002) 79, 9-14. PubMed Europe PubMed DOI

2001

[YEAR:1-6-2001]Melo,R.L., Alves,L.C., Del Nery,E., Juliano,L. and Juliano,M.A. Synthesis and hydrolysis by cysteine and serine proteases of short internally quenched fluorogenic peptides Anal Biochem (1-6-2001) 293, 71-77. PubMed Europe PubMed DOI
[YEAR:3-4-2001]Theodorou,L.G., Lymperopoulos,K., Bieth,J.G. and Papamichael,E.M. Insight into the catalysis of hydrolysis of four newly synthesized substrates by papain: A proton inventory study Biochemistry (3-4-2001) 40, 3996-4004. PubMed Europe PubMed DOI

2000

Achilles,K., Schneider,M., Schirmeister,T. and Otto,H.H. beta-Lactam derivatives as enzyme inhibitors: N-substituted derivatives of (S)-4-oxoazetidine-2-carboxylate as inhibitors of elastase and papain Pharmazie (2000) 55, 798-802. PubMed Europe PubMed I
[YEAR:1-4-2000]Portaro,F.C., Santos,A.B., Cezari,M.H., Juliano,M.A., Juliano,L. and Carmona,E. Probing the specificity of cysteine proteinases at subsites remote from the active site: analysis of P4, P3, P2' and P3' variations in extended substrates Biochem J (1-4-2000) 347, 123-129. PubMed Europe PubMed DOI PMC EPMC
Schirmeister,T. and Peric,M. Aziridinyl peptides as inhibitors of cysteine proteases: effect of a free carboxylic acid function on inhibition Bioorg Med Chem (2000) 8, 1281-1291. PubMed Europe PubMed DOI I
[YEAR:1-8-2000]Tseng,C.C., Tseng,C.P., Levine,M.J. and Bobek,L.A. Differential effect toward inhibition of papain and cathepsin C by recombinant human salivary cystatin SN and its variants produced by a baculovirus system Arch Biochem Biophys (1-8-2000) 380, 133-140. PubMed Europe PubMed DOI
[YEAR:7-7-2000]Xian,M., Chen,X., Liu,Z., Wang,K. and Wang,P.G. Inhibition of papain by S-nitrosothiols. Formation of mixed disulfides J Biol Chem (7-7-2000) 275, 20467-20473. PubMed Europe PubMed DOI I

1999

Ando,R., Sakaki,T., Morinaka,Y., Takahashi,C., Tamao,Y., Yoshii,N., Katayama,S., Saito,K.I., Tokuyama,H., Isaka,M. and Nakamura,E. Cyclopropenone-containing cysteine proteinase inhibitors. Synthesis and enzyme inhibitory activities Bioorg Med Chem (1999) 7, 571-579. PubMed Europe PubMed I
[YEAR:18-5-1999]Czaplewski,C., Grzonka,Z., Jaskolski,M., Kasprzykowski,F., Kozak,M., Politowska,E. and Ciarkowski,J. Binding modes of a new epoxysuccinyl-peptide inhibitor of cysteine proteases. Where and how do cysteine proteases express their selectivity? Biochim Biophys Acta (18-5-1999) 1431, 290-305. PubMed Europe PubMed DOI I
Han,W.G., Tajkhorshid,E. and Suhai,S. QM/MM study of the active site of free papain and of the NMA-papain complex J Biomol Struct Dyn (1999) 16, 1019-1032. PubMed Europe PubMed DOI
[YEAR:26-2-1999]Lecaille,F., Serveau,C., Gauthier,F. and Lalmanach,G. Revisiting the S2 specificity of papain by structural analogs of Phe FEBS Lett (26-2-1999) 445, 311-314. PubMed Europe PubMed DOI P
Matsumoto,K., Mizoue,K., Kitamura,K., Tse,W.C., Huber,C.P. and Ishida,T. Structural basis of inhibition of cysteine proteases by E-64 and its derivatives Biopolymers (1999) 51, 99-107. PubMed Europe PubMed DOI I
[YEAR:13-4-1999]Nagler,D.K., Tam,W., Storer,A.C., Krupa,J.C., Mort,J.S. and Menard,R. Interdependency of sequence and positional specificities for cysteine proteases of the papain family Biochemistry (13-4-1999) 38, 4868-4874. PubMed Europe PubMed DOI
St Hilaire,P.M., Willert,M., Juliano,M.A., Juliano,L. and Meldal,M. Fluorescence-quenched solid phase combinatorial libraries in the characterization of cysteine protease substrate specificity J Comb Chem (1999) 1, 509-523. PubMed Europe PubMed
[YEAR:20-12-1999]Tsuge,H., Nishimura,T., Tada,Y., Asao,T., Turk,D., Turk,V. and Katunuma,N. Inhibition mechanism of cathepsin L-specific inhibitors based on the crystal structure of papain-CLIK148 complex Biochem Biophys Res Commun (20-12-1999) 266, 411-416. PubMed Europe PubMed DOI S I

1998

[YEAR:1-11-1998]Bjork,I., Nordling,K., Raub-Segall,E., Hellman,U. and Olson,S.T. Inactivation of papain by antithrombin due to autolytic digestion: a model of serpin inactivation of cysteine proteinases Biochem J (1-11-1998) 335, 701-709. PubMed Europe PubMed PMC EPMC
[YEAR:5-11-1998]LaLonde,J.M., Zhao,B., Smith,W.W., Janson,C.A., DesJarlais,R.L., Tomaszek,T.A., Carr,T.J., Thompson,S.K., Oh,H.J., Yamashita,D.S., Veber,D.F. and Abdel-Meguid,S.S. Use of papain as a model for the structure-based design of cathepsin K inhibitors: crystal structures of two papain-inhibitor complexes demonstrate binding to S'-subsites J Med Chem (5-11-1998) 41, 4567-4576. PubMed Europe PubMed DOI S I
[YEAR:3-3-1998]Matsumoto,K., Murata,M., Sumiya,S., Mizoue,K., Kitamura,K. and Ishida,T. X-ray crystal structure of papain complexed with cathepsin B-specific covalent-type inhibitor: substrate specificity and inhibitory activity Biochim Biophys Acta (3-3-1998) 1383, 93-100. PubMed Europe PubMed DOI S I

1997

Watts,A., Hafeez,A., Gul,S., Verma,C., Thomas,E.W. and Brocklehurst,K. Effects of site-specific mutations on the kinetically influential ionizations of papain Biochem Soc Trans (1997) 25, 84S-84S. PubMed Europe PubMed
Zheng,Y.J. and Bruice,T.C. Is strong hydrogen bonding in the transition state enough to account for the observed rate acceleration in a mutant of papain? Proc Natl Acad Sci U S A (1997) 94, 4285-4288. PubMed Europe PubMed DOI PMC EPMC I

1996

Kandadai,N.S. and Reddy,M.R. Solution structure of papain as studied by molecular mechanics and molecular dynamics techniques J Comput Chem (1996) 17, 1328-1338. PubMed Europe PubMed DOI S
[YEAR:16-8-1996]Meara,J.P. and Rich,D.H. Mechanistic studies on the inactivation of papain by epoxysuccinyl inhibitors J Med Chem (16-8-1996) 39, 3357-3366. PubMed Europe PubMed DOI I
Ramjee,M.K., Petithory,J.R., McElver,J., Weber,S.C. and Kirsch,J.F. A novel yeast expression/secretion system for the recombinant plant thiol endoprotease propapain Protein Eng (1996) 9, 1055-1061. PubMed Europe PubMed E

1995

Dufour,E., Storer,A.C. and Menard,R. Peptide aldehydes and nitriles as transition state analog inhibitors of cysteine proteases Biochemistry (1995) 34, 9136-9143. PubMed Europe PubMed DOI I
Garcia-Echeverria,C. and Rich,D.H. Kinetic studies of papain: effect of P3' substituents and donor/acceptor pairs of intramolecularly quenched fluorogenic substrates Lett Pept Sci (1995) 2, 77-82. DOI
Hansler,M., Ullmann,G. and Jakubke,H.D. The application of papain, ficin and clostripain in kinetically controlled peptide synthesis in frozen aqueous solutions J Pept Sci (1995) 1, 283-287. PubMed Europe PubMed DOI
[YEAR:4-8-1995]Martichonok,V., Plouffe,C., Storer,A.C., Menard,R. and Jones,J.B. Aziridine analogs of [[trans-(epoxysuccinyl)-L-leucyl]amino]-4-guanidinobutane (E-64) as inhibitors of cysteine proteases J Med Chem (4-8-1995) 38, 3078-3085. PubMed Europe PubMed I
Menard,R., Plouffe,C., Laflamme,P., Vernet,T., Tessier,D.C., Thomas,D.Y. and Storer,A.C. Modification of the electrostatic environment is tolerated in the oxyanion hole of the cysteine protease papain Biochemistry (1995) 34, 464-471. PubMed Europe PubMed
Taylor,M.A., Baker,K.C., Briggs,G.S., Connerton,I.F., Cummings,N.J., Pratt,K.A., Revell,D.F., Freedman,R.B. and Goodenough,P.W. Recombinant pro-regions from papain and papaya proteinase IV are selective high affinity inhibitors of the mature papaya enzymes Protein Eng (1995) 8, 59-62. PubMed Europe PubMed
Vernet,T., Berti,P.J., de Montigny,C., Musil,R., Tessier,D.C., Menard,R., Magny,M.C., Storer,A.C. and Thomas,D.Y. Processing of the papain precursor. The ionization state of a conserved amino acid motif within the pro region participates in the regulation of intramolecular processing J Biol Chem (1995) 270, 10838-10846. PubMed Europe PubMed DOI E
Vernet,T., Tessier,D.C., Chatellier,J., Plouffe,C., Lee,T.S., Thomas,D.Y., Storer,A.C. and Menard,R. Structural and functional roles of asparagine 175 in the cysteine protease papain J Biol Chem (1995) 270, 16645-16652. PubMed Europe PubMed DOI

1994

Matsumoto,K., Murata,M., Sumiya,S., Kitamura,K. and Ishida,T. Clarification of substrate specificity of papain by crystal analyses of complexes with covalent-type inhibitors Biochim Biophys Acta (1994) 1208, 268-276. PubMed Europe PubMed DOI I
Storer,A.C. and Menard,R. Catalytic mechanism in papain family of cysteine peptidases Methods Enzymol (1994) 244, 486-500. PubMed Europe PubMed DOI V

1993

Ando,R., Morinaka,Y., Tokuyama,H., Isaka,M. and Nakamura,E. A new class of proteinase inhibitor. Cyclopropenone-containing inhibitor of papain J Am Chem Soc (1993) 115, 1174-1175. I
Bihovsky,R., Powers,J.C., Kam,C.M., Walton,R. and Loewi,R.C. Further evidence for the importance of free carboxylate in epoxysuccinate inhibitors of thiol proteases J Enzyme Inhib (1993) 7, 15-25. PubMed Europe PubMed I
Menard,R., Carmona,E., Plouffe,C., Bromme,D., Konishi,Y., Lefebvre,J. and Storer,A.C. The specificity of the S1' subsite of cysteine proteases FEBS Lett (1993) 328, 107-110. PubMed Europe PubMed DOI
Schroder,E., Phillips,C., Garman,E., Harlos,K. and Crawford,C. X-ray crystallographic structure of a papain-leupeptin complex FEBS Lett (1993) 315, 38-42. PubMed Europe PubMed DOI S I
Vernet,T., Tessier,D.C., Thomas,D.Y., Chatelier,J., Plouffe,C., Storer,A.C. and Menard,R. Dual role of Asn175 in the catalytic activity and the stability of the cysteine protease papain Protein Eng Des Sel (1993) 6, 52- DOI

1992

Kim,M.J., Yamamoto,D., Matsumoto,K., Inoue,M., Ishida,T., Mizuno,H., Sumiya,S. and Kitamura,K. Crystal structure of papain-E64-c complex. Binding diversity of E64-c to papain S2 and S3 subsites Biochem J (1992) 287, 797-803. PubMed Europe PubMed PMC EPMC S I
[YEAR:20-3-1992]Liu,S. and Hanzlik,R.P. Structure-activity relationships for inhibition of papain by peptide Michael acceptors J Med Chem (20-3-1992) 35, 1067-1075. PubMed Europe PubMed I
Patel,M., Kayani,I.S., Mellor,G.W., Sreedharan,S., Templeton,W., Thomas,E.W., Thomas,M. and Brocklehurst,K. Variation in the P2-S2 stereochemical selectivity towards the enantiomeric N-acetylphenylalanylglycine 4-nitroanilides among the cysteine proteinases papain, ficin and actinidin Biochem J (1992) 281, 553-559. PubMed Europe PubMed PMC EPMC
Pickersgill,R.W., Harris,G.W. and Garman,E. Structure of monoclinic papain at 1.60-A resolution Acta Crystallogr B (1992) 48, 59-67. DOI S
Yamamoto,A., Tomoo,K., Doi,M., Ohishi,H., Inoue,M., Ishida,T., Yamamoto,D., Tsuboi,S., Okamoto,H. and Okada,Y. Crystal structure of papain-succinyl-Gln-Val-Val-Ala-Ala-p-nitroanilide complex at 1.7-A resolution: noncovalent binding mode of a common sequence of endogenous thiol protease inhibitors Biochemistry (1992) 31, 11305-11309. PubMed Europe PubMed DOI S

1991

Gour-Salin,B.J., Storer,A.C., Castelhano,A., Krantz,A. and Robinson,V. Inhibition of papain by peptide nitriles: reactions of external nucleophiles with the thioimidate ester adduct Enzyme Microb Technol (1991) 13, 408-411. I
[YEAR:17-9-1991]Menard,R., Carriere,J., Laflamme,P., Plouffe,C., Khouri,H.E., Vernet,T., Tessier,D.C., Thomas,D.Y. and Storer,A.C. Contribution of the glutamine 19 side chain to transition-state stabilization in the oxyanion hole of papain Biochemistry (17-9-1991) 30, 8924-8928. PubMed Europe PubMed
[YEAR:4-6-1991]Menard,R., Khouri,H.E., Plouffe,C., Laflamme,P., Dupras,R., Vernet,T., Tessier,D.C., Thomas,D.Y. and Storer,A.C. Importance of hydrogen-bonding interactions involving the side chain of Asp158 in the catalytic mechanism of papain Biochemistry (4-6-1991) 30, 5531-5538. PubMed Europe PubMed
Yamamoto,D., Matsumoto,K., Ohishi,H., Ishida,T., Inoue,M., Kitamura,K. and Mizuno,H. Refined X-ray structure of papain.E-64-c complex at 2.1-A resolution J Biol Chem (1991) 266, 14771-14777. PubMed Europe PubMed S I

1990

Hanzlik,R.P., Zygmunt,J. and Moon,J.B. Reversible covalent binding of peptide nitriles to papain Biochim Biophys Acta (1990) 1035, 62-70. PubMed Europe PubMed DOI I
Hu,L.Y. and Abeles,R.H. Inhibition of cathepsin B and papain by peptidyl alpha-keto esters, alpha-keto amides, alpha-diketones, and alpha-keto acids Arch Biochem Biophys (1990) 281, 271-274. PubMed Europe PubMed DOI I
Menard,R., Khouri,H.E., Plouffe,C., Dupras,R., Ripoll,D., Vernet,T., Tessier,D.C., Lalberte,F., Thomas,D.Y. and Storer,A.C. A protein engineering study of the role of aspartate 158 in the catalytic mechanism of papain Biochemistry (1990) 29, 6706-6713. PubMed Europe PubMed DOI
Thiele,U., Assfalg-Machleidt,I., Machleidt,W. and Auerswald,E.A. N-Terminal variants of recombinant stefin B: effect on affinity for papain and cathepsin B Biol Chem Hoppe Seyler (1990) 371 Suppl, 125-136. PubMed Europe PubMed
Yamamoto,D., Ishida,T. and Inoue,M. A comparison between the binding modes of a substrate and inhibitor to papain as observed in complex crystal structures Biochem Biophys Res Commun (1990) 171, 711-716. PubMed Europe PubMed DOI S I
Yamamoto,D., Matsumoto,K., Ohishi,H., Ishida,T., Inoue,M., Kitamura,K. and Hanada,K. The importance of Val-157 hydrophobic interaction for papain inhibitory activity of an epoxysuccinyl amino acid derivative. A structure-activity relationship based on the crystal structure of the papain-E-64-c complex FEBS Lett (1990) 263, 134-136. PubMed Europe PubMed DOI S I

1989

[YEAR:1-1-1989]Brocklehurst,K., O'Driscoll,M., Kowlessur,D., Phillips,I.R., Templeton,W., Thomas,E.W., Topham,C.M. and Wharton,C.W. The interplay of electrostatic and binding interactions determining active centre chemistry and catalytic activity in actinidin and papain Biochem J (1-1-1989) 257, 309-310. PubMed Europe PubMed PMC EPMC
Buttle,D.J., Kembhavi,A.A., Sharp,S.L., Shute,R.E., Rich,D.H. and Barrett,A.J. Affinity purification of the novel cysteine proteinase papaya proteinase IV, and papain from papaya latex Biochem J (1989) 261, 469-476. PubMed Europe PubMed I
Machleidt,W., Thiele,U., Laber,B., Assfalg-Machleidt,I., Esterl,A., Wiegand,G., Kos,J., Turk,V. and Bode,W. Mechanism of inhibition of papain by chicken egg white cystatin. Inhibition constants of N-terminally truncated forms and cyanogen bromide fragments of the inhibitor FEBS Lett (1989) 243, 234-238. PubMed Europe PubMed DOI
[YEAR:13-3-1989]Matsumoto,K., Yamamoto,D., Ohishi,H., Tomoo,K., Ishida,T., Inoue,M., Sadatome,T., Kitamura,K. and Mizuno,H. Mode of binding of E-64-c, a potent thiol protease inhibitor, to papain as determined by X-ray crystal analysis of the complex FEBS Lett (13-3-1989) 245, 177-180. PubMed Europe PubMed DOI S I
[YEAR:25-1-1989]Schultz,R.M., Varma-Nelson,P., Ortiz,R., Kozlowski,K.A., Orawski,A.T., Pagast,P. and Frankfater,A. Active and inactive forms of the transition-state analog protease inhibitor leupeptin: explanation of the observed slow binding of leupeptin to cathepsin B and papain J Biol Chem (25-1-1989) 264, 1497-1507. PubMed Europe PubMed I
Varughese,K.I., Ahmed,F.R., Carey,P.R., Hasnain,S., Huber,C.P. and Storer,A.C. Crystal structure of a papain-E-64 complex Biochemistry (1989) 28, 1330-1332. PubMed Europe PubMed S I

1988

[YEAR:1-12-1988]Brocklehurst,K., Brocklehurst,S.M., Kowlessur,D., O'Driscoll,M., Patel,G., Salih,E., Templeton,W., Thomas,E., Topham,C.M. and Willenbrock,F. Supracrystallographic resolution of interactions contributing to enzyme catalysis by use of natural structural variants and reactivity-probe kinetics Biochem J (1-12-1988) 256, 543-558. PubMed Europe PubMed PMC EPMC
Chen,S.-T., Li,K.-F. and Wang,K.-T. Papain catalyzed synthesis of aspartyl dipeptides J Chin Chem Soc (1988) 35, 207-211. DOI
Lindahl,P., Alriksson,E., Jornvall,H. and Bjork,I. Interaction of the cysteine proteinase inhibitor chicken cystatin with papain Biochemistry (1988) 27, 5074-5082. PubMed Europe PubMed
[YEAR:15-8-1988]Pickersgill,R.W., Goodenough,P.W., Sumner,I.G. and Collins,M.E. The electrostatic fields in the active-site clefts of actinidin and papain Biochem J (15-8-1988) 254, 235-238. PubMed Europe PubMed PMC EPMC

1987

Brocklehurst,K., Willenbrock,F. and Salih,E. Cysteine proteinases (1987) , 39-158.
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1986

Cohen,L.W., Coghlan,V.M. and Dihel,L.C. Cloning and sequencing of papain-encoding cDNA Gene (1986) 48, 219-227. PubMed Europe PubMed
Goodenough,P.W. and Owen,J. Chromatographic and electrophoretic analyses of papaya proteinases Phytochemistry (1986) 26, 75-79.
McKee,R.A., Adams,S., Matthews,J.A., Smith,C.J. and Smith,H. Molecular cloning of two cysteine proteinases from paw-paw (Carica papaya) Biochem J (1986) 237, 105-110. PubMed Europe PubMed
[YEAR:1-4-1986]Migliorini,M. and Creighton,D.J. Active-site ionizations of papain. An evaluation of the potentiometric difference titration method Eur J Biochem (1-4-1986) 156, 189-192. PubMed Europe PubMed
Moon,J.B., Coleman,R.S. and Hanzlik,R.P. Reversible covalent inhibition of papain by a peptide nitrile. 13C NMR evidence for a thioimidate ester adduct J Am Chem Soc (1986) 108, 1350-1351. I
Oka,T. and Morihara,K. Inactivation of papain with chloroacetyl amino acid derivatives Agric Biol Chem (1986) 50, 519-520. DOI I
Thompson,S.A., Andrews,P.R. and Hanzlik,R.P. Carboxyl-modified amino acids and peptides as protease inhibitors J Med Chem (1986) 29, 104-111. PubMed Europe PubMed I

1985

[YEAR:29-1-1985]Asboth,B., Stokum,E., Khan,I.U. and Polgar,L. Mechanism of action of cysteine proteinases: oxyanion binding site is not essential in the hydrolysis of specific substrates Biochemistry (29-1-1985) 24, 606-609. PubMed Europe PubMed
Kamphuis,I.G., Drenth,J. and Baker,E.N. Thiol proteases. Comparative studies based on the high-resolution structures of papain and actinidin, and on amino acid sequence information for cathepsins B and H, and stem bromelain J Mol Biol (1985) 182, 317-329. PubMed Europe PubMed S

1984

[YEAR:1-11-1984]Brocklehurst,K., Carey,P.R., Lee,H.H., Salih,E. and Storer,A.C. Comparative resonance Raman spectroscopic and kinetic studies of acyl-enzymes involving papain, actinidin and papaya peptidase II Biochem J (1-11-1984) 223, 649-657. PubMed Europe PubMed PMC EPMC
[YEAR:1-6-1984]Brocklehurst,K., Salih,E. and Lodwig,T.S. Differences between the electric fields of the catalytic sites of papain and actinidin detected by using the thiol-located nitrobenzofurazan label as a spectroscopic reporter group Biochem J (1-6-1984) 220, 609-612. PubMed Europe PubMed PMC EPMC
Hanzlik,R.P. and Thompson,S.A. Vinylogous amino acid esters: a new class of inactivators for thiol proteases J Med Chem (1984) 27, 711-712. PubMed Europe PubMed I
Kamphuis,I.G., Kalk,K.H., Swarte,M.B. and Drenth,J. Structure of papain refined at 1.65 A resolution J Mol Biol (1984) 179, 233-256. PubMed Europe PubMed S
Sasmito,T., Demeester,J. and Lauwers,A. The inhibition of papain Arch Physiol Biochem (1984) 92, BP24-BP25. DOI I
[YEAR:1-5-1984]Schack,P. and Kaarsholm,N.C. Subsite differences between the active centres of papaya peptidase A and papain as revealed by affinity chromatography. Purification of papaya peptidase A by ionic-strength-dependent affinity adsorption on an immobilized peptide inhibitor of papain Biochem J (1-5-1984) 219, 727-733. PubMed Europe PubMed PMC EPMC

1983

[YEAR:1-6-1983]Brocklehurst,K., Willenbrock,S.J. and Salih,E. Effects of conformational selectivity and of overlapping kinetically influential ionizations on the characteristics of pH-dependent enzyme kinetics. Implications of free-enzyme pKa variability in reactions of papain for its catalytic mechanism Biochem J (1-6-1983) 211, 701-708. PubMed Europe PubMed PMC EPMC
Carey,P.R., Ozaki,Y. and Storer,A.C. Comparison of the substrate conformations in the active sites of papain, chymopapain, ficin and bromelain by resonance Raman spectroscopy Biochem Biophys Res Commun (1983) 117, 725-731. PubMed Europe PubMed
Gamcsik,M.P., Malthouse,J.P.G., Primrose,W.U., Mackenzie,N.E., Boyd,A.S.F., Russell,R.A. and Scott,A.I. Structure and stereochemistry of tetrahedral inhibitor complexes of papain by direct NMR observation J Am Chem Soc (1983) 105, 6324-6325.
Lynn,K.R. Definition of the site of reactivity of the ancestral protease of the papain type Phytochemistry (1983) 22, 2485-2487. DOI P
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1982

Baines,B.S. and Brocklehurst,K. Isolation and characterization of the four major cysteine-proteinase components of the latex of Carica papaya L. Reactivity characteristics towards 2,2-dipyridyldisulfide of the thiol groups of papain, chymopapains A and B, and papaya peptidase A J Protein Chem (1982) 1, 119-139. DOI
Brocklehurst,K., Mushiri,S.M., Patel,G. and Willenbrock,F. Evidence for a close similarity in the catalytic sites of papain and ficin in near-neutral media despite differences in acidic and alkaline media. Kinetics of the reactions of papain and ficin with chloroacetate Biochem J (1982) 201, 101-104. PubMed Europe PubMed PMC EPMC

1981

Brocklehurst,K., Baines,B.S. and Malthouse,J.P. Differences in the interaction of the catalytic groups of the active centres of actinidin and papain. Rapid purification of fully active actinidin by covalent chromatography and characterization of its active centre by use of two- protonic- state reactivity probes Biochem J (1981) 197, 739-746. PubMed Europe PubMed PMC EPMC
Frankfater,A. and Kuppy,T. Mechanism of association of N-acetyl-L-phenylalanylglycinal to papain Biochemistry (1981) 20, 5517-5524. PubMed Europe PubMed I
Tamai,M., Adachi,T., Oguma,K., Morimoto,S., Hanada,K., Ohmura,S. and Ohzeki,M. Relationship between structure and papain inhibitory activity of epoxysuccinyl amino acid derivatives Agric Biol Chem (1981) 45, 675-679. DOI I

1980

Valeri,A.M., Wilson,S.M. and Feinman,R.D. Reaction of antithrombin with proteases. Evidence for a specific reaction with papain Biochim Biophys Acta (1980) 614, 526-533. PubMed Europe PubMed

1979

[YEAR:1-2-1979]Baines,B.S. and Brocklehurst,K. A necessary modification to the preparation of papain from any high-quality latex of Carica papaya and evidence for the structural integrity of the enzyme produced by traditional methods Biochem J (1-2-1979) 177, 541-548. PubMed Europe PubMed PMC EPMC
Caygill,J.C. Sulphydryl plant proteases Enzyme Microb Technol (1979) 1, 233-242. V
Funk,M.O., Nakagawa,Y., Skochdopole,J. and Kaiser,E.T. Affinity chromatographic purification of papain. A reinvestigation Int J Pept Protein Res (1979) 13, 296-303. PubMed Europe PubMed I
Henes,J.B., Mattis,J.A. and Fruton,J.S. Interaction of papain with derivatives of phenylalanylglycinal: fluorescence studies Proc Natl Acad Sci U S A (1979) 76, 1131-1134. PubMed Europe PubMed PMC EPMC I
[YEAR:1-8-1979]Polgar,L. Deuterium isotope effects on papain acylation. Evidence for lack of general base catalysis and for enzyme-leaving-group interaction Eur J Biochem (1-8-1979) 98, 369-374. PubMed Europe PubMed

1978

[YEAR:3-7-1978]Allen,K.G., Stewart,J.A., Johnson,P.E. and Wettlaufer,D.G. Identification of the functional ionic groups of papain by pH/rate profile analysis Eur J Biochem (3-7-1978) 87, 575-582. PubMed Europe PubMed
Butler,J.E. and Kennedy,N. The differential enzyme susceptibility of bovine immunoglobulin G1 and immunglobulin G2 to pepsin and papain Biochim Biophys Acta (1978) 535, 125-137. PubMed Europe PubMed P

1977

Bendall,M.R., Cartwright,I.L., Clark,P.I., Lowe,G. and Nurse,D. Inhibition of papain by N-acyl-aminoacetaldehydes and N-acyl-aminopropanones. Evidence for hemithioacetal formation by a cross-saturation technique in nuclear-magnetic-resonance spectroscopy Eur J Biochem (1977) 79, 201-209. PubMed Europe PubMed I
Clark,P.I. and Lowe,G. Chemical mutations of papain. The preparation of ser 25- and gly 25-papain J Chem Soc Chem Commun (1977) 1977, 923-924. DOI
[YEAR:3-10-1977]Halasz,P. and Polgar,L. Negatively charged reactants as probes in the study of the essential mercaptide-imidazolium ion-pair of thiolenzymes Eur J Biochem (3-10-1977) 79, 491-494. PubMed Europe PubMed I
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[YEAR:10-10-1977]Mattis,J.A., Henes,J.B. and Fruton,J.S. Interaction of papain with derivatives of phenylalanylglycinal J Biol Chem (10-10-1977) 252, 6776-6782. PubMed Europe PubMed I
Wang,A.C. and Wang,I.Y. Cleavage sites of human IgGI immunolglobulin by papain Immunochemistry (1977) 14, 197-200. PubMed Europe PubMed P

1976

Drenth,J., Kalk,K.H. and Swen,H.M. Binding of chloromethyl ketone substrate analogues to crystalline papain Biochemistry (1976) 15, 3731-3738. PubMed Europe PubMed I
[YEAR:11-12-1976]Halasz,P. and Polgar,L. Effect of the immediate environment on the reactivity of the essential -SH group of papain Eur J Biochem (11-12-1976) 71, 571-575. PubMed Europe PubMed I
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1975

Shipton,M., Kierstan,M.P.J., Malthouse,J.P.G., Stuchbury,T. and Brocklehurst,K. The case for assigning a value of approximately 4 to pKa(I) of the essential histidine cysteine interactive systems of papain, bromelain and ficin FEBS Lett (1975) 50, 365-368. PubMed Europe PubMed DOI

1974

Burke,D.E., Lewis,S.D. and Shafer,J.A. A two-step procedure for purification of papain from extract of papaya latex Arch Biochem Biophys (1974) 164, 30-36. PubMed Europe PubMed
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1973

Brocklehurst,K., Carlsson,J., Kierstan,M.P. and Crook,E.M. Covalent chromatography. Preparation of fully active papain from dried papaya latex Biochem J (1973) 133, 573-584. PubMed Europe PubMed PMC EPMC
Brocklehurst,K. and Little,G. Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2'-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols Biochem J (1973) 133, 67-80. PubMed Europe PubMed PMC EPMC
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1972

Brocklehurst,K. and Little,G. Reactivities of the various protonic states in the reactions of papain and of L-cysteine with 2,2'- and with 4,4'- dipyridyl disulphide: evidence for nucleophilic reactivity in the un-ionized thiol group of the cysteine-25 residue of papain occasioned by its interaction with the histidine-159-asparagine-175 hydrogen-bonded system Biochem J (1972) 128, 471-474. PubMed Europe PubMed PMC EPMC
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1971

Drenth,J., Jansonius,J.N., Koekoek,R. and Wolthers,B.G. The structure of papain Adv Protein Chem (1971) 25, 79-115. PubMed Europe PubMed DOI S
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Lowe,G. and Yuthavong,Y. pH-Dependence and structure-activity relationships in the papain-catalysed hydrolysis of anilides Biochem J (1971) 124, 117-122. PubMed Europe PubMed PMC EPMC

1970

Anderson,B.M. and Vasini,E.C. Nonpolar effects in reactions of the sulfhydryl group of papain Biochemistry (1970) 9, 3348-3352. PubMed Europe PubMed I
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1969

Husain,S.S. and Lowe,G. Completion of the amino acid sequence of papain Biochem J (1969) 114, 279-288. PubMed Europe PubMed PMC EPMC

1968

Arnon,R. and Shapira,E. Comparison between the antigenic structure of mutually related enzymes. A study with papain and chymopapain Biochemistry (1968) 7, 4196-4202. PubMed Europe PubMed
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1967

Arnon,R. and Shapira,E. Antibodies to papain. A selective fractionation according to inhibitory capacity Biochemistry (1967) 6, 3942-3950. PubMed Europe PubMed
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1966

Bender,M.L., Begue-Canton,M.L., Blakeley,R.L., Brubacher,L.J., Feder,J., Gunter,C.R., Kezdy,F.J., Killheffer,J.V., Jr., Marshall,T.H., Miller,C.G., Roeske,R.W. and Stoops,J.K. The determination of the concentration of hydrolytic enzyme solutions: alpha-chymotrypsin, trypsin, papain, elastase, subtilisin, and acetylcholinesterase J Am Chem Soc (1966) 88, 5890-5913. PubMed Europe PubMed
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1965

Arnon,R. The reactoin of papain with antipapain Immunochemistry (1965) 2, 107-114. PubMed Europe PubMed DOI
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Husain,S.S. and Lowe,G. The location of the active centre cysteine residue in the primary sequence of papain Chem Commun (London) (1965) 15, 345-346. DOI
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1964

Light,A. The reaction of iodoacetate and bromoacetate with papain Biochem Biophys Res Commun (1964) 17, 781-785. I
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1963

Sanner,T. and Pihl,A. Studies on the active -SH group of papain and on the mechanism of papain activation by thiols J Biol Chem (1963) 238, 165-171. PubMed Europe PubMed
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1962

Ozawa,K., Ohnishi,T. and Tanaka,S. Activation and inhibition of papain J Biochem (1962) 51, 372-374. PubMed Europe PubMed

1958

Smith,E.L. Active site of papain and covalent high-energy bonds of proteins J Biol Chem (1958) 233, 1392-1397. PubMed Europe PubMed

1957

Kimmel,J.R. and Smith,E.L. The properties of papain Adv Enzymol Relat Subj Biochem (1957) 19, 267-334. PubMed Europe PubMed

1956

Davis,N.C. Action of proteolytic enzymes on some peptides and derivatives containing histidine J Biol Chem (1956) 223, 935-947. PubMed Europe PubMed P

1955

Kimmel,J.R., Thompson,E.O. and Smith,E.L. Crystalline papain. V. Cysteic acid and cysteic acid peptides from oxidized papain J Biol Chem (1955) 217, 151-159. PubMed Europe PubMed
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1954

Kimmel,J.R. and Smith,E.L. Crystalline papain. I. Preparation, specificity, and activation J Biol Chem (1954) 207, 515-531. PubMed Europe PubMed
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1953

Abelin,I. and Pfister,H. [Determination and activation of papain] Hoppe Seylers Z Physiol Chem (1953) 295, 323-332. PubMed Europe PubMed
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1950

Fox,S.W. and Pettinga,C.W. Enzymic synthesis of peptide bonds; preferences of papain within the monoaminomonocarboxylic acid series Arch Biochem (1950) 25, 21-29. PubMed Europe PubMed
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1949

Hanson,H.T. and Smith,E.L. Papain resolution of DL-tryptophan; optical specificity of carboxypeptidase J Biol Chem (1949) 179, 815-818. PubMed Europe PubMed

1946

Petermann,M.L. The splitting of human gamma globulin antibodies by papain and bromelin J Am Chem Soc (1946) 68, 106-113. PubMed Europe PubMed

1940

Berger,J. and Asenjo,C.F. Anthelmintic activity of crystalline papain Science (1940) 91, 387-388. PubMed Europe PubMed DOI

1939

[YEAR:20-9-1938]Anson,M.L. The estimation of pepsin, trypsin, papain and cathepsin with hemoglobin J Gen Physiol (20-9-1938) 22, 79-89. PubMed Europe PubMed DOI A

1937

Anson,M.L. The estimation of papain with hemoglobin J Gen Physiol (1937) 20, 561-563. PubMed Europe PubMed DOI A
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1936

Bergmann,M. and Ross,W.F. On proteolytic enzymes. X. The enzymes of papain and their activation J Biol Chem (1936) 114, 717-726.

1935

Bergmann,M., Zervas,L. and Fruton,J.S. On proteolytic enzymes. VI. On the specificity of papain J Biol Chem (1935) 111, 225-244.

1933

Calvery,H.O. Crystalline egg albumin. The hydrolysis of crystalline egg albumin by pepsin, papain-hydrocyanic acid, and pancreatic proteinase and the subsequent action of some other enzymes on the hydrolysis products produced by these enzymes J Biol Chem (1933) 102, 73-88.

1885

Martin,S.H.C. The nature of papain and its action on vegetable proteids J Physiol (1885) 6, 336-360. PubMed Europe PubMed DOI
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1884

Martin,S.H.C. Papain digestion J Physiol (1884) 5, 213-230.

1879

Wurtz,A. and Bouchut,E. Sur le ferment digestif du Carica papaya C R Acad Sci Hebd Seances Acad Sci D (1879) 89, 425-430.

1878

Wittmack,H. The fermentative action of the juice of the fruit of Carica papaya Pharm J Trans (1878) 9, 449-