Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 1 |
unknown peptidase |
1-375 |
P |
|
NT |
<%Agarwal et al., 2012[]%> |
| 11 |
caspase-1 |
2-375 |
P |
|
|
Mashima et al., 1997 |
| 17 |
peptidyl-Lys metallopeptidase |
1-375 |
N |
|
MS |
|
| 18 |
granzyme A |
1-375 |
N |
|
|
Van Damme et al., 2010 |
| 18 |
trypsin 1 |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 25 |
unknown peptidase |
1-375 |
P |
|
MS |
Van Damme et al., 2005 |
| 28 |
trypsin 1 |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 39 |
thrombin |
25-57 |
N |
|
MS |
Schilling & Overall, 2008 |
| 40 |
matrix metallopeptidase-2 |
25-57 |
N |
|
MS |
Schilling & Overall, 2008 |
| 43 |
elastase-2 |
2-375 |
P |
|
|
Yamashita et al., 2001 |
| 47 |
matrix metallopeptidase-2 |
31-65 |
N |
|
MS |
Schilling & Overall, 2008 |
| 49 |
peptidyl-Lys metallopeptidase |
1-375 |
N |
|
MS |
|
| 50 |
trypsin 1 |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 51 |
caspase-4 |
1-375 |
N |
|
MS |
Agard et al., 2010 |
| 51 |
granzyme B (Homo sapiens-type) |
1-375 |
N |
|
MS |
Van Damme et al., 2010 |
| 57 |
glutamyl endopeptidase I |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 60 |
peptidyl-Lys metallopeptidase |
1-375 |
N |
|
MS |
|
| 61 |
trypsin 1 |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 62 |
trypsin 1 |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 63 |
matrix metallopeptidase-2 |
62-72 |
N |
|
MS |
Schilling & Overall, 2008 |
| 65 |
chymotrypsin A (cattle-type) |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 68 |
trypsin 1 |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 72 |
glutamyl endopeptidase I |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 74 |
matrix metallopeptidase-2 |
68-84 |
N |
|
MS |
Schilling & Overall, 2008 |
| 77 |
cathepsin S |
69-84 |
N |
|
MS |
Biniossek et al., 2011 |
| 84 |
trypsin 1 |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 84 |
lysyl endopeptidase (bacteria) |
2-375 |
N |
|
|
Ott et al., 1996 |
| 118 |
lysyl endopeptidase (bacteria) |
2-375 |
N |
|
|
Ott et al., 1996 |
| 183 |
trypsin 1 |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 191 |
trypsin 1 |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 191 |
lysyl endopeptidase (bacteria) |
2-375 |
N |
|
|
Ott et al., 1996 |
| 196 |
trypsin 1 |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 206 |
trypsin 1 |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 215 |
trypsin 1 |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 222 |
HIV-1 retropepsin |
215-238 |
N |
|
MS |
Schilling & Overall, 2008 |
| 222 |
caspase-6 |
2-375 |
P |
|
MS |
Klaiman et al., 2008 |
| 222 |
granzyme B (Homo sapiens-type) |
1-375 |
N |
|
MS |
Plasman et al., 2011 |
| 223 |
HIV-1 retropepsin |
215-238 |
N |
|
MS |
Schilling & Overall, 2008 |
| 226 |
glutamyl endopeptidase I |
225-238 |
N |
|
MS |
Schilling & Overall, 2008 |
| 227 |
HIV-1 retropepsin |
225-238 |
N |
|
MS |
Schilling & Overall, 2008 |
| 227 |
cathepsin G |
225-238 |
N |
|
MS |
Schilling & Overall, 2008 |
| 238 |
trypsin 1 |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 238 |
lysyl endopeptidase (bacteria) |
2-375 |
N |
|
|
Ott et al., 1996 |
| 241 |
chymotrypsin A (cattle-type) |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 244 |
caspase-1 |
2-375 |
P |
normal turnover |
|
Mashima et al., 1997 |
| 244 |
caspase-3 |
2-375 |
P |
|
|
Mashima et al., 1997 |
| 244 |
caspase-7 |
241-254 |
N |
|
MS |
Schilling & Overall, 2008 |
| 244 |
matrix metallopeptidase-2 |
241-255 |
N |
|
MS |
Schilling & Overall, 2008 |
| 244 |
glutamyl endopeptidase I |
241-254 |
N |
|
MS |
Schilling & Overall, 2008 |
| 254 |
trypsin 1 |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 255 |
chymotrypsin A (cattle-type) |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 260 |
matrix metallopeptidase-2 |
256-284 |
N |
|
MS |
Schilling & Overall, 2008 |
| 270 |
cathepsin S |
257-279 |
N |
|
MS |
Biniossek et al., 2011 |
| 284 |
trypsin 1 |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 290 |
trypsin 1 |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 292 |
matrix metallopeptidase-2 |
291-312 |
N |
|
MS |
Schilling & Overall, 2008 |
| 296 |
HIV-1 retropepsin |
291-312 |
N |
|
MS |
Schilling & Overall, 2008 |
| 296 |
matrix metallopeptidase-2 |
291-312 |
N |
|
MS |
Schilling & Overall, 2008 |
| 296 |
matrix metallopeptidase-11 |
1-375 |
N |
|
|
Kleifeld et al., 2010 |
| 312 |
trypsin 1 |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 314 |
peptidyl-Lys metallopeptidase |
1-375 |
N |
|
MS |
|
| 315 |
trypsin 1 |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 325 |
peptidyl-Lys metallopeptidase |
1-375 |
N |
|
MS |
|
| 326 |
trypsin 1 |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 358 |
peptidyl-Lys metallopeptidase |
1-375 |
N |
|
MS |
|
| 359 |
trypsin 1 |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
| 362 |
HIV-1 retropepsin |
359-372 |
N |
|
MS |
Schilling & Overall, 2008 |
| 363 |
cathepsin L |
360-372 |
N |
|
MS |
Biniossek et al., 2011 |
| 363 |
caspase-6 |
2-375 |
P |
|
MS |
Klaiman et al., 2008 |
| 372 |
trypsin 1 |
2-375 |
N |
|
MS |
Schilling & Overall, 2008 |
