Substrates for peptidase S08.054: endopeptidase K

Summary Alignment Sequences Sequence features Distribution Structure Literature Substrates

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	Evidence	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference	CutDB
Ac-Tyr-OEt [ATEE]			Ac-Tyr+OEt	S		-	-	Ac	Tyr	OEt	-	-	-	Saenger, 2004
alpha-hemolysin precursor	P09616	27-319	peptide-Gly159+Gly-peptide	N		Gly	Lys	Ile	Gly	Gly	Leu	Ile	Gly	Walker et al., 1992	17519
alpha-hemolysin precursor	P09616	27-319	peptide-Leu161+Ile-peptide	N		Ile	Gly	Gly	Leu	Ile	Gly	Ala	Asn	Walker et al., 1992	17520
alpha-hemolysin precursor	P09616	27-319	peptide-Ala164+Asn-peptide	N		Leu	Ile	Gly	Ala	Asn	Val	Ser	Ile	Walker et al., 1992	17517
alpha-hemolysin precursor	P09616	27-319	peptide-Asn165+Val-peptide	N		Ile	Gly	Ala	Asn	Val	Ser	Ile	Gly	Walker et al., 1992	17518
alpha-lactalbumin	P00711	20-142	peptide-Gln58+Ala-peptide	N		Tyr	Asp	Thr	Gln	Ala	Ile	Val	Gln	Polverino de Laureto et al., 1999
alpha-lactalbumin	P00711	20-142	peptide-Gln62+Asn-peptide	N		Ala	Ile	Val	Gln	Asn	Asn	Asp	Ser	Polverino de Laureto et al., 1999
alpha-lactalbumin	P00711	20-142	peptide-Phe72+Gln-peptide	N		Tyr	Gly	Leu	Phe	Gln	Ile	Asn	Asn	Polverino de Laureto et al., 1999
alpha-lactalbumin	P00711	20-142	peptide-Ser53+Gly-peptide	N		Phe	His	Thr	Ser	Gly	Tyr	Asp	Thr	Polverino de Laureto et al., 1999
alpha-lactalbumin	P00711	20-142	peptide-Asn75+Asn-peptide	N		Phe	Gln	Ile	Asn	Asn	Lys	Ile	Trp	Polverino de Laureto et al., 1999
alpha-lactalbumin	P00711	20-142	peptide-Ile114+Leu-peptide	N		Val	Lys	Lys	Ile	Leu	Asp	Lys	Val	Polverino de Laureto et al., 1999
alpha-lactalbumin	P00711	20-142	peptide-Lys117+Val-peptide	N		Ile	Leu	Asp	Lys	Val	Gly	Ile	Asn	Polverino de Laureto et al., 1999
alpha-synuclein	P37840	1-140	peptide-Lys43+Thr-peptide	N		Val	Gly	Ser	Lys	Thr	Lys	Glu	Gly	Levin etal, 2008
alpha-synuclein	P37840	1-140	peptide-Asp98+Gln-peptide	N		Val	Lys	Lys	Asp	Gln	Leu	Gly	Lys	Levin etal, 2008
alpha-synuclein	P37840	1-140	peptide-Ala53+Thr-peptide	N		His	Gly	Val	Ala	Thr	Val	Ala	Glu	Levin etal, 2008
alpha-synuclein	P37840	1-140	peptide-Thr92+Gly-peptide	N		Ala	Ala	Ala	Thr	Gly	Phe	Val	Lys	Levin etal, 2008
alpha-synuclein	P37840	1-140	peptide-Thr54+Val-peptide	N		Gly	Val	Ala	Thr	Val	Ala	Glu	Lys	Levin etal, 2008
alpha-synuclein	P37840	1-140	peptide-Ala91+Thr-peptide	N		Ile	Ala	Ala	Ala	Thr	Gly	Phe	Val	Levin etal, 2008
alpha-synuclein	P37840	1-140	peptide-Ala89+Ala-peptide	N		Gly	Ser	Ile	Ala	Ala	Ala	Thr	Gly	Levin etal, 2008
alpha-synuclein	P37840	1-140	peptide-Val70+Val-peptide	N		Gly	Gly	Ala	Val	Val	Thr	Gly	Val	Levin etal, 2008
alpha-synuclein	P37840	1-140	peptide-Ala18+Ala-peptide	N		Val	Val	Ala	Ala	Ala	Glu	Lys	Thr	Levin etal, 2008
alpha-synuclein	P37840	1-140	peptide-Gly7+Leu-peptide	N		Phe	Met	Lys	Gly	Leu	Ser	Lys	Ala	Levin etal, 2008
alpha-synuclein	P37840	1-140	Met1+Ala-peptide	N		-	-	-	Met	Asp	Val	Phe	Met	Levin etal, 2008
avidin precursor	P02701	25-152	peptide-Thr64+Ser-peptide	N		Val	Thr	Ala	Thr	Ser	Asn	Glu	Ile	Ellison et al., 1995
avidin precursor	P02701	25-152	peptide-Asn66+Glu-peptide	N		Ala	Thr	Ser	Asn	Glu	Ile	Lys	Glu	Ellison et al., 1995
Catalase HPII	P21179	1-753	Peptide-Gln308+Lys-Peptide	N	NT	Asp	Glu	Ala	Gln	Lys	Leu	Thr	Gly	Chelikani et al., 2003
Catalase HPII	P21179	1-753	Peptide-Ser587+Leu-Peptide	N	NT	Lys	Asp	Pro	Ser	Leu	Ser	Leu	Tyr	Chelikani et al., 2003
Catalase HPII	P21179	1-753	Peptide-Gly74+Ser-Peptide	N	NT	Val	Arg	Lys	Gly	Ser	Glu	Asn	Tyr	Chelikani et al., 2003
cytosol alanyl aminopeptidase	P55786	1-919	peptide-Glu535+Asp-peptide	N		Tyr	Val	Gly	Glu	Asp	Cys	Pro	Gln	Ma et al., 2003	20473
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn+Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N		-	Phe	Val	Asn	Gln	His	Leu	Cya	Kraus et al., 1976
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln+His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N		Phe	Val	Asn	Gln	His	Leu	Cya	Gly	Kraus et al., 1976
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His+Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N		Val	Asn	Gln	His	Leu	Cya	Gly	Ser	Kraus et al., 1976
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya+Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N		Tyr	Leu	Val	Cya	Gly	Glu	Arg	Gly	Kraus et al., 1976
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser+His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N		Leu	Cya	Gly	Ser	His	Leu	Val	Glu	Kraus et al., 1976
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu+Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N		Ala	Leu	Tyr	Leu	Val	Cya	Gly	Glu	Kraus et al., 1976
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu+Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N		His	Leu	Val	Glu	Ala	Leu	Tyr	Leu	Kraus et al., 1976
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu+Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N		Val	Glu	Ala	Leu	Tyr	Leu	Val	Cya	Kraus et al., 1976
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr+Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N		Glu	Ala	Leu	Tyr	Leu	Val	Cya	Gly	Kraus et al., 1976
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu+Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N		Gly	Ser	His	Leu	Val	Glu	Ala	Leu	Kraus et al., 1976
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya+Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N		Val	His	Leu	Cya	Gly	Ser	His	Ser	Kraus et al., 1976
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly+Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N		Leu	Val	Cya	Gly	Glu	Arg	Gly	Phe	Kraus et al., 1976
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu+Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N		Val	Cya	Gly	Glu	Arg	Gly	Phe	Phe	Kraus et al., 1976
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe+Phe-Tyr-Thr-Pro-Lys-Ala	N		Glu	Arg	Gly	Phe	Phe	Tyr	Thr	Pro	Kraus et al., 1976
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe+Tyr-Thr-Pro-Lys-Ala	N		Arg	Gly	Phe	Phe	Tyr	Thr	Pro	Lys	Kraus et al., 1976
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr+Thr-Pro-Lys-Ala	N		Gly	Phe	Phe	Tyr	Thr	Pro	Lys	Ala	Kraus et al., 1976
Light-harvesting protein B-800/850 beta chain	P07368	1-49	Peptide-Leu11+Ser-Peptide	P	NT	Pro	Ser	Gly	Leu	Ser	Leu	Lys	Glu	Tadros et al., 1986
Suc-Ala-Ala-Pro-Phe-NHPhNO2			Suc-Ala-Ala-Pro-Phe+NHPhNO2	S		Ala	Ala	Pro	Met	NAN	-	-	-	Larsen et al., 2006