Substrates for peptidase S01.157: chymotrypsin C

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Pharma

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	Evidence	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference	CutDB
Bz-Leu-OEt			Bz-Leu+OEt	S		-	-	Bz	Leu	OEt	-	-	-	Szilagyi, 2004
carboxypeptidase A1	P15085	17-110	peptide-Phe105+Ala-peptide	P	MS	Glu	Gln	Met	Phe	Ala	Phe	Arg	Ser	Szmola et al., 2011
carboxypeptidase A1	P15085	17-110	peptide-Gln103+Met-peptide	P	MS	Glu	Gln	Glu	Gln	Met	Phe	Ala	Phe	Szmola et al., 2011
carboxypeptidase A1	P15085	17-110	peptide-Met104+Phe-peptide	P	MS	Gln	Glu	Gln	Met	Phe	Ala	Phe	Arg	Szmola et al., 2011
carboxypeptidase A1	P15085	17-110	peptide-Leu96+Leu-peptide	P	MS	Val	Gln	Ser	Leu	Leu	Asp	Glu	Glu	Szmola et al., 2011
carboxypeptidase A1	P15085	17-110	peptide-Gln94+Ser-peptide	P	MS	Glu	Asp	Val	Gln	Ser	Leu	Leu	Asp	Szmola et al., 2011
carboxypeptidase A2	P48052	19-114	peptide-Phe108+Asn-peptide	P	MS	Glu	Met	Leu	Phe	Asn	Arg	Arg	Arg	Szmola et al., 2011
carboxypeptidase A2	P48052	19-114	peptide-Met106+Leu-peptide	P	MS	Asn	Glu	Glu	Met	Leu	Phe	Asn	Arg	Szmola et al., 2011
carboxypeptidase A2	P48052	19-114	peptide-Leu107+Phe-peptide	P	MS	Glu	Glu	Met	Leu	Phe	Asn	Arg	Arg	Szmola et al., 2011
carboxypeptidase A2	P48052	19-114	peptide-Leu98+Leu-peptide	P	MS	Val	Gln	Val	Leu	Leu	Asp	Lys	Glu	Szmola et al., 2011
carboxypeptidase A2	P48052	19-114	peptide-Gln96+Val-peptide	P	MS	Glu	Asp	Val	Gln	Val	Leu	Leu	Asp	Szmola et al., 2011
cationic trypsin	P07477	24-247	peptide-Leu81+Glu-peptide	P	NT	Ile	Glu	Val	Leu	Glu	Gly	Asn	Glu	Szmola & Sahin-Toth, 2007	24028
cationic trypsin	P07477	24-81	peptide-Leu41+Asn-peptide	P	NT	Gln	Val	Ser	Leu	Asn	Ser	Gly	Tyr	Szmola & Sahin-Toth, 2007	24027
Glucagon	P01274	53-81	His-Ser-Gln55+Gly-Peptide	N	NT	-	His	Ser	Gln	Gly	Thr	Phe	Thr	Folk et al., 1965
Glucagon	P01274	53-81	Peptide-Phe58+Thr-Peptide	N	NT	Gln	Gly	Thr	Phe	Thr	Ser	Asp	Tyr	Folk et al., 1965
Glucagon	P01274	53-81	Peptide-Tyr62+Ser-Peptide	N	NT	Thr	Ser	Asp	Tyr	Ser	Lys	Tyr	Leu	Folk et al., 1965
Glucagon	P01274	53-81	Peptide-Tyr65+Leu-Peptide	N	NT	Tyr	Ser	Lys	Tyr	Leu	Asp	Ser	Arg	Folk et al., 1965
Glucagon	P01274	53-81	Peptide-Leu66+Asp-Peptide	N	NT	Ser	Lys	Tyr	Leu	Asp	Ser	Arg	Arg	Folk et al., 1965
Glucagon	P01274	53-81	Peptide-Gln72+Asp-Peptide	N	NT	Arg	Arg	Ala	Gln	Asp	Phe	Val	Gln	Folk et al., 1965
Glucagon	P01274	53-81	Peptide-Phe74+Val-Peptide	N	NT	Ala	Gln	Asp	Phe	Val	Gln	Trp	Leu	Folk et al., 1965
Glucagon	P01274	53-81	Peptide-Gln76+Trp-Peptide	N	NT	Asp	Phe	Val	Gln	Trp	Leu	Met	Asn	Folk et al., 1965
Glucagon	P01274	53-81	Peptide-Trp77+Leu-Met-Asn-Thr	N	NT	Phe	Val	Gln	Trp	Leu	Met	Asn	Thr	Folk et al., 1965
Glucagon	P01274	53-81	Peptide-Leu78+Met-Asn-Thr	N	NT	Val	Gln	Trp	Leu	Met	Asn	Thr	-	Folk et al., 1965
Glucagon	P01274	53-81	Peptide-Met79+Asn-Thr	N	NT	Gln	Trp	Leu	Met	Asn	Thr	-	-	Folk et al., 1965
insulin A chain (oxidized)	P01317	85-105	Gly-Ile-Val-Glu-Gln+Cya-Cya-Ala-Ser-Val-Cya-Ser-Leu-Tyr-Gln-Leu-Glu-Asn-Tyr-Cya-Asn	N		Ile	Val	Glu	Gln	Cya	Cya	Ala	Ser	Fricke et al., 2001
insulin A chain (oxidized)	P01317	85-105	Gly-Ile-Val-Glu-Gln-Cya-Cya-Ala-Ser-Val-Cya+Ser-Leu-Tyr-Gln-Leu-Glu-Asn-Tyr-Cya-Asn	N		Ala	Ser	Val	Cya	Ser	Leu	Tyr	Gln	Fricke et al., 2001
insulin A chain (oxidized)	P01317	85-105	Gly-Ile-Val-Glu-Gln-Cya-Cya-Ala-Ser-Val-Cya-Ser-Leu+Tyr-Gln-Leu-Glu-Asn-Tyr-Cya-Asn	N		Val	Cya	Ser	Leu	Tyr	Gln	Leu	Glu	Fricke et al., 2001
insulin A chain (oxidized)	P01317	85-105	Gly-Ile-Val-Glu-Gln-Cya-Cya-Ala-Ser-Val-Cya-Ser-Leu-Tyr+Gln-Leu-Glu-Asn-Tyr-Cya-Asn	N		Cya	Ser	Leu	Tyr	Gln	Leu	Glu	Asn	Fricke et al., 2001
insulin A chain (oxidized)	P01317	85-105	Gly-Ile-Val-Glu-Gln-Cya-Cya-Ala-Ser-Val-Cya-Ser-Leu-Tyr-Gln+Leu-Glu-Asn-Tyr-Cya-Asn	N		Ser	Leu	Tyr	Gln	Leu	Glu	Asn	Tyr	Fricke et al., 2001
insulin A chain (oxidized)	P01317	85-105	Gly-Ile-Val-Glu-Gln-Cya-Cya-Ala-Ser-Val-Cya-Ser-Leu-Tyr-Gln-Leu+Glu-Asn-Tyr-Cya-Asn	N		Leu	Tyr	Gln	Leu	Glu	Asn	Tyr	Cya	Fricke et al., 2001
insulin A chain (oxidized)	P01317	85-105	Gly-Ile-Val-Glu-Gln-Cya-Cya-Ala-Ser-Val-Cya-Ser-Leu-Tyr-Gln-Leu-Glu-Asn+Tyr-Cya-Asn	N		Gln	Leu	Glu	Asn	Tyr	Cya	Asn	-	Fricke et al., 2001
insulin A chain (oxidized)	P01317	85-105	Gly-Ile-Val-Glu-Gln-Cya-Cya-Ala-Ser-Val-Cya-Ser-Leu-Tyr-Gln-Leu-Glu-Asn-Tyr+Cya-Asn	N		Leu	Glu	Asn	Tyr	Cya	Asn	-	-	Fricke et al., 2001
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu+Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N		Asn	Gln	His	Leu	Cya	Gly	Ser	His	Szilagyi, 2004
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu+Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N		Val	Glu	Ala	Leu	Tyr	Leu	Val	Cya	Szilagyi, 2004
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln+His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N		Phe	Val	Asn	Gln	His	Leu	Cya	Gly	Szilagyi, 2004
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr+Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N		Glu	Ala	Leu	Tyr	Leu	Val	Cya	Gly	Szilagyi, 2004
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu+Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N		Ala	Leu	Tyr	Leu	Val	Cya	Gly	Glu	Szilagyi, 2004
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe+Phe-Tyr-Thr-Pro-Lys-Ala	N		Glu	Arg	Gly	Phe	Phe	Tyr	Thr	Pro	Szilagyi, 2004
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe+Tyr-Thr-Pro-Lys-Ala	N		Arg	Gly	Phe	Phe	Tyr	Thr	Pro	Lys	Szilagyi, 2004
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr+Thr-Pro-Lys-Ala	N		Gly	Phe	Phe	Tyr	Thr	Pro	Lys	Ala	Szilagyi, 2004
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu+Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N	NT	Gly	Ser	His	Leu	Val	Glu	Ala	Leu	Folk et al., 1965
Oxytocin (oxidized)	P01178	20-28	Cys-Tyr-Ile-Gln23+Asn-Peptide	N	NT	Cys	Tyr	Ile	Gln	Asn	Cys	Pro	Leu	Folk et al., 1965
Oxytocin (oxidized)	P01178	20-28	Peptide-Leu27+Gly-NH2	N	NT	Asn	Cys	Pro	Leu	Gly	NH2	-	-	Folk et al., 1965
Suc-Ala-Ala-Pro-Phe-NHPhNO2			Suc-Ala-Ala-Pro-Phe+NHPhNO2	S		Ala	Ala	Pro	Phe	NAN	-	-	-	Szilagyi, 2004
Trypsin-1 precursor	P07477	16-247	Ala-Pro-Phe18+Asp-peptide	P	NT	-	Ala	Pro	Phe	Asp	Asp	Asp	Asp	Nemoda & Sahin-T�th, 2006
Trypsin-2	P07478	16-247	Ala-Pro-Phe18+Asp-peptide	P	NT	-	Ala	Pro	Phe	Asp	Asp	Asp	Asp	Nemoda & Sahin-T�th, 2006