Substrates for peptidase M72.001: peptidyl-Asp metallopeptidase

Summary Alignment Sequences Sequence features Distribution Literature Substrates

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	Evidence	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference	CutDB
aspartyl bond			peptide+Asp-peptide	N		-	-	-	NPe	Asp	CPe	-	-	Hagmann, 2004
baltergin ({Bothrops alternatus})	P0C6R9	1-196	peptide-Cys69+Asp-peptide	N		Met	Ser	Glu	Cys	Asp	Pro	Ala	Glu	Souza et al., 2000
baltergin ({Bothrops alternatus})	P0C6R9	1-196	peptide-Leu95+Asp-peptide	N		Gln	Pro	Cys	Leu	Asp	Asn	Tyr	Gly	Souza et al., 2000
baltergin ({Bothrops alternatus})	P0C6R9	1-196	peptide-Ala120+Asp-peptide	N		Leu	Phe	Gly	Ala	Asp	Val	Tyr	Glu	Souza et al., 2000
baltergin ({Bothrops alternatus})	P0C6R9	1-196	peptide-Lys131+Asp-peptide	N		Ser	Cys	Phe	Lys	Asp	Asn	Gln	Lys	Souza et al., 2000
bdellin-KL	Q9NCC2	1-155	peptide-Ala18+Asp-peptide	N		Ala	Ile	Asn	Ala	Asp	Thr	Glu	Cys	Kim et al., 2001
bdellin-KL	Q9NCC2	1-155	peptide-Glu66+Asp-peptide	N		Glu	His	His	Glu	Asp	Glu	His	His	Kim et al., 2001
cysteyl bond			peptide+Cya-peptide	N		-	-	-	NPe	Cya	CPe	-	-	Hagmann, 2004
glucagon	P01274	53-81	His-Ser-Gln-Gly-Thr-Phe-Thr-Ser+Asp-Tyr-Ser-Lys-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr	N		Thr	Phe	Thr	Ser	Asp	Tyr	Ser	Lys	Hagmann, 2004	17179
glucagon	P01274	53-81	His-Ser-Gln-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Lys-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gln+Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr	N		Arg	Arg	Ala	Gln	Asp	Phe	Val	Gln	Hagmann, 2004	17181
glucagon	P01274	53-81	His-Ser-Gln-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Lys-Tyr-Leu+Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr	N		Ser	Lys	Tyr	Leu	Asp	Ser	Arg	Arg	Hagmann, 2004	17180
insulin-like growth factor binding protein 5	P24593	21-272	peptide-Arg114+Asp-peptide	P		Lys	Ile	Glu	Arg	Asp	Ser	Arg	Glu	Kalus et al., 1998
insulin-like growth factor binding protein 5	P24593	21-272	peptide-Lys154+Asp-peptide	P		Ala	Val	Lys	Lys	Asp	Arg	Arg	Lys	Kalus et al., 1998
lysozyme C	P00698	18-147	peptide-Leu35+Asp-peptide	N	MS	Arg	His	Gly	Leu	Asp	Asn	Tyr	Arg	Kameoka et al., 2003
lysozyme C	P00698	18-147	peptide-Thr65+Asp-peptide	N	MS	Asn	Arg	Asn	Thr	Asp	Gly	Ser	Thr	Kameoka et al., 2003
lysozyme C	P00698	18-147	peptide-Thr69+Asp-peptide	N	MS	Asp	Gly	Ser	Thr	Asp	Tyr	Gly	Ile	Kameoka et al., 2003
lysozyme C	P00698	18-147	peptide-Asn83+Asp-peptide	N	MS	Trp	Trp	Cys	Asn	Asp	Gly	Arg	Thr	Kameoka et al., 2003
lysozyme C	P00698	18-147	peptide-Ser104+Asp-peptide	N	MS	Leu	Leu	Ser	Ser	Asp	Ile	Thr	Ala	Kameoka et al., 2003
lysozyme C	P00698	18-147	peptide-Ser118+Asp-peptide	N	MS	Lys	Ile	Val	Ser	Asp	Gly	Asn	Gly	Kameoka et al., 2003
lysozyme C	P00698	18-147	peptide-Thr136+Asp-peptide	N	MS	Cys	Lys	Gly	Thr	Asp	Val	Gln	Ala	Kameoka et al., 2003
thymosin beta-4	P62328	2-44	peptide-Pro5+Asp-peptide	P	CS	Ser	Asp	Lys	Pro	Asp	Met	Ala	Glu	Cavasin et al., 2004