Substrates for peptidase A01.041: BACE2 g.p. (Homo sapiens)

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Pharma

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	Evidence	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference	CutDB
amyloid beta A4 protein	P05067	18-770	peptide-Phe673+Phe-peptide	P		Lys	Leu	Val	Phe	Phe	Ala	Glu	Asp	Fluhrer et al., 2002	4043
amyloid beta A4 protein	P05067	18-770	peptide-Phe674+Ala-peptide	P		Leu	Val	Phe	Phe	Ala	Glu	Asp	Val	Farzan et al., 2000	4044
Amyloid beta A4 protein	P05067	672-694	peptide-His684+His-peptide	N	MS	Tyr	Glu	Val	His	His	Gln	Lys	Leu	Kim et al., 2002
Amyloid beta A4 protein	P05067	672-694	peptide-His685+Gln-peptide	N	MS	Glu	Val	His	His	Gln	Lys	Leu	Val	Kim et al., 2002
Amyloid beta A4 protein	P05067	672-694	peptide-Gln686+Lys-peptide	N	MS	Val	His	His	Gln	Lys	Leu	Val	Phe	Kim et al., 2002
Amyloid beta A4 protein	P05067	672-694	peptide-Lys687+Leu-peptide	N	MS	His	His	Gln	Lys	Leu	Val	Phe	Phe	Kim et al., 2002
Amyloid beta A4 protein	P05067	667-678	peptide-Met671+Asp-peptide	N	MS	Glu	Val	Lys	Met	Asp	Ala	Glu	Phe	Kim et al., 2002
Amyloid beta A4 protein	P05067	667-678	peptide-Asp672+Ala-peptide	N	MS	Val	Lys	Met	Asp	Ala	Glu	Phe	Arg	Kim et al., 2002
Amyloid beta A4 protein	P05067	667-678	peptide-Glu674+Phe-Arg-His-Asp	N	MS	Met	Asp	Ala	Glu	Phe	Arg	His	Asp	Kim et al., 2002
Amyloid beta A4 protein	P05067	667-678	peptide-Arg676+His-Asp	N	MS	Ala	Glu	Phe	Arg	His	Asp	-	-	Kim et al., 2002
Bradykinin-like neuropeptide	Q00676	81-91	peptide-Arg85+Gly-peptide	N	MS	Lys	Arg	Ser	Arg	Gly	Pro	Ser	Pro	Kim et al., 2002
Bradykinin-like neuropeptide	Q00676	81-91	peptide-Pro89+Arg-Arg	N	MS	Gly	Pro	Ser	Pro	Arg	Arg	-	-	Kim et al., 2002
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu+Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N	MS	Gly	Ser	His	Leu	Val	Glu	Ala	Leu	Kim et al., 2002
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu+Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N	MS	His	Leu	Val	Glu	Ala	Leu	Tyr	Leu	Kim et al., 2002
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu+Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N	MS	Val	Glu	Ala	Leu	Tyr	Leu	Val	Cya	Kim et al., 2002
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr+Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N	MS	Glu	Ala	Leu	Tyr	Leu	Val	Cya	Gly	Kim et al., 2002
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly+Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N	MS	Gly	Glu	Arg	Gly	Phe	Phe	Tyr	Thr	Kim et al., 2002
Mastoparan-L	P01514	1-14	Ile-Asn-Leu3+Lys-peptide	N	MS	-	Ile	Asn	Leu	Lys	Ala	Leu	Ala	Kim et al., 2002
Mastoparan-L	P01514	1-14	Ile-Asn-Leu-Lys4+Ala-peptide	N	MS	Ile	Asn	Leu	Lys	Ala	Leu	Ala	Ala	Kim et al., 2002
Mastoparan-L	P01514	1-14	peptide-Leu9+Ala-peptide	N	MS	Leu	Ala	Ala	Leu	Ala	Lys	Lys	Ile	Kim et al., 2002
Mastoparan-L	P01514	1-14	peptide-Ala10+Lys-Lys-Ile-Leu	N	MS	Ala	Ala	Leu	Ala	Lys	Lys	Ile	Leu	Kim et al., 2002
memapsin-1 precursor	Q9Y5Z0	21-518	peptide-Leu42+Ala-peptide	P		Gly	Leu	Ala	Leu	Ala	Leu	Glu	Pro	Hussain et al., 2001	4045
Neurogenic locus notch homolog protein 1	P46531	1750-1759	Peptide-Leu1756+Ser-Arg-Lys	P	MS	Gly	Val	Leu	Leu	Ser	Arg	Lys	-	Kim et al., 2002
Neurogenic locus notch homolog protein 1	P46531	1750-1759	Peptide-Ser1757+Arg-Lys	P	MS	Val	Leu	Leu	Ser	Arg	Lys	-	-	Kim et al., 2002
Proenkephalin-A	P01211	126-135	Gly-Glu-Val-Leu129+Gly-peptide	N	MS	Gly	Glu	Val	Leu	Gly	Lys	Arg	Tyr	Kim et al., 2002
Proenkephalin-A	P01211	126-135	peptide-Gly130+Lys-peptide	N	MS	Glu	Val	Leu	Gly	Lys	Arg	Tyr	Gly	Kim et al., 2002
Proenkephalin-A	P01211	126-135	peptide-Lys131+Arg-Tyr-Gly-Gly	N	MS	Val	Leu	Gly	Lys	Arg	Tyr	Gly	Gly	Kim et al., 2002
Proenkephalin-A	P01211	125-137	peptide-Arg132+Tyr-peptide	N	MS	Leu	Gly	Lys	Arg	Tyr	Gly	Gly	Phe	Kim et al., 2002
Proenkephalin-A	P01211	125-137	peptide-Tyr133+Gly-Gly-Phe-Met	N	MS	Gly	Lys	Arg	Tyr	Gly	Gly	Phe	Met	Kim et al., 2002
Proenkephalin-A	P01211	125-137	Gly125+Gly-peptide	N	MS	-	-	-	Gly	Gly	Glu	Val	Leu	Kim et al., 2002
Serum albumin	P02768	166-174	Ile-Ala-Arg-Arg169+His-Peptide	N	MS	Ile	Ala	Arg	Arg	His	Pro	Tyr	Phe	Kim et al., 2002
Serum albumin	P02768	166-174	peptide-His170+Pro-Tyr-Phe-Tyr	N	MS	Ala	Arg	Arg	His	Pro	Tyr	Phe	Tyr	Kim et al., 2002
Serum albumin	P02768	166-174	peptide-Tyr172+Phe-Tyr	N	MS	Arg	His	Pro	Tyr	Phe	Tyr	-	-	Kim et al., 2002