Substrates for peptidase A01.006: chymosin

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	Evidence	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference	CutDB
alpha-2-macroglobulin	P01023	24-1474	peptide-Phe707+Tyr-peptide	P		Arg	Val	Gly	Phe	Tyr	Glu	Ser	Asp	Mortensen et al., 1981
alpha-2-macroglobulin	P01023	24-1474	peptide-Val731+Arg-peptide	P		Thr	Glu	Thr	Val	Arg	Lys	Tyr	Phe	Mortensen et al., 1981
alpha-2-macroglobulin	P01023	24-1474	peptide-Tyr734+Phe-peptide	P		Val	Arg	Lys	Tyr	Phe	Pro	Glu	Thr	Mortensen et al., 1981
alpha-S1-casein	P02662	16-214	peptide-Phe23+Phe24-peptide	P		Leu	Leu	Arg	Phe	Phe	Val	Ala	Pro	Kobayashi, 2004	15745
beta-casein	P02666	16-224	peptide-Leu139+Leu-peptide	P		Pro	Leu	Pro	Leu	Leu	Gln	Ser	Trp	Kobayashi, 2004	15747
beta-casein	P02666	16-224	peptide-Leu165+Ser166-peptide	P		Val	Leu	Ser	Leu	Ser	Gln	Ser	Lys	Kobayashi, 2004	15748
beta-casein	P02666	16-224	peptide-Ala189+Phe190-peptide	P		Pro	Ile	Gln	Ala	Phe	Leu	Leu	Tyr	Kobayashi, 2004	15746
chymosin	P00794	59-381	peptide-Tyr301+Gly-peptide	P	NT	Gln	Asn	Gln	Tyr	Asp	Gly	Phe	Asp	Danley et al., 1988
chymosin	P00794	302-381	Asp-Glu+Asp-peptide	P	NT	-	Gly	Glu	Phe	Asp	Ile	Asp	Cys	Danley et al., 1988
chymosin	P00794	17-381	peptide-Phe43+Leu-peptide	P	NT	Leu	Glu	Asp	Phe	Leu	Gln	Lys	Gln	Pedersen et al., 1979
His-Pro-His-Pro-His-Leu-Ser-Phe-Met-Ala-Ile-Pro-Pro-Lys-Lys			His-Pro-His-Pro-His-Leu-Ser-Phe+Met-Ala-Ile-Pro-Pro-Lys-Lys	N		His	Leu	Ser	Phe	Met	Ala	Ile	Pro	Foltmann & Szecsi, 2004
kappa casein precursor	Q8WN74	22-192	peptide-Phe126+Met-peptide	N		His	Leu	Ser	Phe	Met	Ala	Ile	Pro
kappa casein precursor	P19442	22-234	peptide-Phe118+Met-peptide	N		His	Pro	Ser	Phe	Met	Ala	Ile	Leu		15752
kappa casein precursor	P07498	21-182	peptide-Phe117+Ile-peptide	N		His	Pro	Ser	Phe	Ile	Ala	Ile	Pro		15750
kappa casein precursor	P06796	22-181	peptide-Phe118+Leu-peptide	N		Asn	Pro	Ser	Phe	Leu	Ala	Met	Pro		15751
kappa casein precursor	P04468	22-178	peptide-Phe115+Leu-peptide	N		Asn	Pro	Ser	Phe	Leu	Ala	Ile	Pro		15749
kappa casein precursor	P02669	22-192	peptide-Phe126+Met-peptide	N		His	Leu	Ser	Phe	Met	Ala	Ile	Pro		15753
kappa-casein	P02668	22-190	peptide-Phe105+Met106-peptide	P		His	Leu	Ser	Phe	Met	Ala	Ile	Pro	Foltmann & Szecsi, 2004
Lys-Pro-Ile-Glu-Phe-Nph-Arg-Leu			Lys-Pro-Ile-Glu-Phe+Nph-Arg-Leu	S		Pro	Ile	Glu	Phe	Nph	Arg	Leu	-	Oda et al., 1992
Pro-Thr-Glu-Phe-Nph-Arg-Leu			Pro-Thr-Glu-Phe+Nph-Arg-Leu	N		Pro	Thr	Glu	Phe	Nph	Arg	Leu	-	Sidrach et al., 2005