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Literature for peptidase A01.006: chymosin

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

(Topics flags: S Structure, A Assay, K Knockout, P Specificity, E Expression, V Review, M Mutation, I Inhibitor. To select only the references relevant to a single topic, click the link above. See explanation.)

    2025
  1. Zheng,Y., Wang,S., Deng,Y., Hu,P., Xue,Q., Li,J., Lei,L., Chan,Z., Yang,J. and Peng,W.
    Enhanced production of recombinant calf chymosin in Kluyveromyces lactis via CRISPR-Cas9 engineering
    Bioresour Technol419, 132116-132116. PubMed  Europe PubMed DOI
    2. 2024
  2. Amjad,H., Saleem,F., Ahmad,M., Nisar,U. and Arshad Dar,H.
    Comprehensive bioinformatics-based annotation and functional characterization of bovine chymosin protein revealed novel biological insights
    Food Chem (Oxf)8, 100191-100191. PubMed  Europe PubMed DOI
    3. 2023
  3. Balabova,D.V., Belash,E.A., Belenkaya,S.V., Shcherbakov,D.N., Belov,A.N., Koval,A.D., Mironova,A.V., Bondar,A.A., Volosnikova,E.A., Arkhipov,S.G., Sokolova,O.O., Chirkova,V.Y. and Elchaninov,V.V.
    Biochemical Properties of a Promising Milk-Clotting Enzyme, Moose (Alces alces) Recombinant Chymosin
    Foods12, PubMed  Europe PubMed DOI
    4. 2022
  4. Wang,N., Yang,C., Peng,H., Guo,W., Wang,M., Li,G. and Liu,D.
    The introduction of an N-glycosylation site into prochymosin greatly enhances its production and secretion by Pichia pastoris
    Microb Cell Fact21, 177-177. PubMed  Europe PubMed DOI
    5. 2021
  5. Al-Zoreky,N.S. and Almathen,F.S.
    Using recombinant camel chymosin to make white soft cheese from camel milk
    Food Chem337, 127994-127994. PubMed  Europe PubMed DOI
    6. 2020
  6. Belenkaya,S.V., Bondar,A.A., Kurgina,T.A., Elchaninov,V.V., Bakulina,A.Y., Rukhlova,E.A., Lavrik,O.I., Ilyichev,A.A. and Shcherbakov,D.N.
    Characterization of the Altai Maral Chymosin Gene, Production of a Chymosin Recombinant Analog in the Prokaryotic Expression System, and Analysis of Its Several Biochemical Properties
    Biochemistry (Mosc)85, 781-791. PubMed  Europe PubMed DOI
    7. 2019
  7. Gumus,P. and Hayaloglu,A.A.
    Effects of blends of camel and calf chymosin on proteolysis, residual coagulant activity, microstructure, and sensory characteristics of Beyaz peynir
    J Dairy Sci102, 5945-5956. PubMed  Europe PubMed DOI
  8. Soltani,M., Sahingil,D., Gokce,Y. and Hayaloglu,A.A.
    Effect of blends of camel chymosin and microbial rennet (Rhizomucor miehei) on chemical composition, proteolysis and residual coagulant activity in Iranian ultrafiltered white cheese
    J Food Sci Technol56, 589-598. PubMed  Europe PubMed DOI
    9. 2018
  9. Ansari,S.M., Sorensen,J., Schiott,B. and Palmer,D.S.
    On the effect of mutations in bovine or camel chymosin on the thermodynamics of binding kappa-caseins
    Proteins86, 75-87. PubMed  Europe PubMed DOI  M
    10. 2017
  10. Liu,W.G., Wang,Y.P., Zhang,Z.J., Wang,M., Lv,Q.X., Liu,H.W., Meng,L.C. and Lu,M.
    Generation and characterization of caprine chymosin in corn seed
    Protein Expr Purif135, 78-82. PubMed  Europe PubMed DOI  E
  11. Lopes-Marques,M., Ruivo,R., Fonseca,E., Teixeira,A. and Castro,L.F.C.
    Unusual loss of chymosin in mammalian lineages parallels neo-natal immune transfer strategies
    Mol Phylogenet Evol116, 78-86. PubMed  Europe PubMed DOI
    12. 2016
  12. Ansari,S.M., Coletta,A., Kirkeby Skeby,K., Sorensen,J., Schiott,B. and Palmer,D.S.
    Allosteric-activation mechanism of bovine chymosin revealed by bias-exchange metadynamics and molecular dynamics simulations
    J Phys Chem B120, 10453-10462. PubMed  Europe PubMed DOI
  13. Espinoza-Molina,J.A., Acosta-Muniz,C.H., Sepulveda,D.R., Zamudio-Flores,P.B. and Rios-Velasco,C.
    Codon optimization of the "Bos taurus chymosin" gene for the production of recombinant chymosin in Pichia pastoris
    Mol Biotechnol58, 657-664. PubMed  Europe PubMed DOI  E
  14. Huang,J., Zhu,Y., Sun,B., Yao,Y. and Liu,J.
    Determination of the protonation state of the Asp dyad: conventional molecular dynamics versus thermodynamic integration
    J Mol Model22, 58-58. PubMed  Europe PubMed DOI
  15. Luo,F., Jiang,W.H., Yang,Y.X., Li,J. and Jiang,M.F.
    Cloning and Expression of Yak Active Chymosin in Pichia pastoris
    Asian-Australas J Anim Sci29, 1363-1370. PubMed  Europe PubMed DOI  E
  16. Noseda,D.G., Recupero,M., Blasco,M., Bozzo,J. and Galvagno,M.A.
    Production in stirred-tank bioreactor of recombinant bovine chymosin B by a high-level expression transformant clone of Pichia pastoris
    Protein Expr Purif123, 112-121. PubMed  Europe PubMed DOI  E
  17. Rengifo,A.F.C., Ferreira,G.M.D., Ferreira,G.M.D., daSilva,M.C.H., dePaula Rezende,J., dosSantos Pires,A.C. and daSilva,L.H.M.
    Driving forces for chymosin partitioning on the macromolecule-salt aqueous two phase system
    Food Bioprod Process100, 361-371. DOI
  18. Tyagi,A., Kumar,A., Yadav,A.K., ChandolaSaklani,A., Grover,S. and Batish,V.K.
    Functional expression of recombinant goat chymosin in Pichia pastoris bioreactor cultures: a commercially viable alternate
    LWT - Food Sci Technol69, 217-224. DOI  E
  19. Ulusu,Y., Senturk,S.B., Kudug,H. and Gokce,I.
    Expression, purification, and characterization of bovine chymosin enzyme using an inducible pTOLT system
    Prep Biochem Biotechnol46, 596-601. PubMed  Europe PubMed DOI  E
  20. Wei,Z.-Y., Zhang,Y.-Y., Wang,Y.-P., Fan,M.-X., Zhong,X.-F., Xu,N., Lin,F. and Xing,S.-C.
    Production of bioactive recombinant bovine chymosin in tobacco plants
    Int J Mol Sci17, PubMed  Europe PubMed DOI  E
    21. 2015
  21. Jensen,J.L., Jacobsen,J., Moss,M.L., Rasmussen,F., Qvist,K.B., Larsen,S. and van den Brink,J.M.
    The function of the milk-clotting enzymes bovine and camel chymosin studied by a fluorescence resonance energy transfer assay
    J Dairy Sci98, 2853-2860. PubMed  Europe PubMed DOI  A
  22. Rolet-Repecaud,O., Arnould,C., Dupont,D., Gavoye,S., Beuvier,E. and Achilleos,C.
    Development and evaluation of a monoclonal antibody-based inhibition ELISA for the quantification of chymosin in solution
    J Agric Food Chem63, 4799-4804. PubMed  Europe PubMed DOI
  23. Wang,N., Wang,K.Y., Li,G., Guo,W. and Liu,D.
    Expression and characterization of camel chymosin in Pichia pastoris
    Protein Expr Purif111, 75-81. PubMed  Europe PubMed DOI  E
    24. 2014
  24. Noseda,D.G., Blasco,M., Recupero,M. and Galvagno,M.A.
    Bioprocess and downstream optimization of recombinant bovine chymosin B in Pichia (Komagataella) pastoris under methanol-inducible AOXI promoter
    Protein Expr Purif104C, 85-91. PubMed  Europe PubMed DOI  E
    25. 2013
  25. Langholm Jensen,J., Molgaard,A., Navarro Poulsen,J.C., Harboe,M.K., Simonsen,J.B., Lorentzen,A.M., Hjerno,K., van den Brink,J.M., Qvist,K.B. and Larsen,S.
    Camel and bovine chymosin: the relationship between their structures and cheese-making properties
    Acta Crystallogr D Biol Crystallogr69, 901-913. PubMed  Europe PubMed DOI  S
  26. Noseda,D.G., Recupero,M.N., Blasco,M., Ortiz,G.E. and Galvagno,M.A.
    Cloning, expression and optimized production in a bioreactor of bovine chymosin B in Pichia (Komagataella) pastoris under AOX1 promoter
    Protein Expr Purif92, 235-244. PubMed  Europe PubMed DOI  E
  27. Yoon,J., Kikuma,T., Maruyama,J. and Kitamoto,K.
    Enhanced production of bovine chymosin by autophagy deficiency in the filamentous fungus Aspergillus oryzae
    PLoS ONE8, e62512-e62512. PubMed  Europe PubMed DOI  E
    28. 2012
  28. Garcia,V., Rovira,S., Teruel,R., Boutoial,K., Rodriguez,J., Roa,I. and Lopez,M.B.
    Effect of vegetable coagulant, microbial coagulant and calf rennet on physicochemical, proteolysis, sensory and texture profiles of fresh goats cheese
    Dairy Sci Technol92, 691-707. DOI
  29. Hsieh,J.F. and Pan,P.H.
    Proteomic profiling of the coagulation of milk proteins induced by chymosin
    J Agric Food Chem60, 2039-2045. PubMed  Europe PubMed DOI
  30. Moller,K.K., Rattray,F.P., Sorensen,J.C. and Ardo,Y.
    Comparison of the hydrolysis of bovine kappa-casein by camel and bovine chymosin: a kinetic and specificity study
    J Agric Food Chem60, 5454-5460. PubMed  Europe PubMed DOI
  31. Moller,K.K., Rattray,F.P. and Ardo,Y.
    Camel and bovine chymosin hydrolysis of bovine alpha(S1)- and beta-caseins studied by comparative peptide mapping
    J Agric Food Chem60, 11421-11432. PubMed  Europe PubMed DOI  P
  32. Szecsi,P.B. and Harboe,M.
    Chymosin
    [ISSN:978-0-12-407744-7]3, 37-42. DOI
    33. 2011
  33. Barac,M., Cabrilo,S., Pesic,M., Stanojevic,S., Pavlicevic,M., Macej,O. and Ristic,N.
    Functional properties of pea (Pisum sativum, L.) protein isolates modified with chymosin
    Int J Mol Sci12, 8372-8387. PubMed  Europe PubMed DOI
  34. Heinrich,M. and Kulozik,U.
    Study of chymosin hydrolysis of casein micelles under ultra high pressure: effect on re-association upon pressure release
    Int Dairy J21, 664-669. DOI
  35. Jacob,M., Jaros,D. and Rohm,H.
    Proteolytic activity of traditional calf rennet and selected milk coagulant substitutes
    Milchwissenschaft66, 240-244.
  36. Sorensen,J., Palmer,D.S., Qvist,K.B. and Schiott,B.
    Initial stage of cheese production: a molecular modeling study of bovine and camel chymosin complexed with peptides from the chymosin-sensitive region of kappa-casein
    J Agric Food Chem59, 5636-5647. PubMed  Europe PubMed DOI
    37. 2010
  37. Kageyama,H., Ueda,H., Tezuka,T., Ogasawara,A., Narita,Y., Kageyama,T. and Ichinose,M.
    Differences in the P1' substrate specificities of pepsin A and chymosin
    J Biochem147, 167-174. PubMed  Europe PubMed DOI  P
  38. Kumar,A., Grover,S., Sharma,J. and Batish,V.K.
    Chymosin and other milk coagulants: sources and biotechnological interventions
    Crit Rev Biotechnol30, 243-258. PubMed  Europe PubMed DOI  V
  39. Palmer,D.S., Christensen,A.U., Sorensen,J., Celik,L., Qvist,K.B. and Schiott,B.
    Bovine chymosin: a computational study of recognition and binding of bovine kappa-casein
    Biochemistry49, 2563-2573. PubMed  Europe PubMed DOI
  40. Yuan,W., Ke,T., Du,M., Chu,X., Hu,F. and Hui,F.
    [Gene synthesis of the bovine prochymosin gene and high-level expression in Kluyvermyces lactis]
    Sheng Wu Gong Cheng Xue Bao26, 1281-1286. PubMed  Europe PubMed  E
    41. 2009
  41. Bansal,N., Drake,M.A., Piraino,P., Broe,M.L., Harboe,M., Fox,P.F. and McSweeney,P.L.H.
    Suitability of recombinant camel (Camelus dromedarius) chymosin as a coagulant for Cheddar cheese
    Int Dairy J19, 510-517. DOI
  42. Zhang,L., Jiang,Y., Zhang,J. and Yang,Z.
    [Recombinant expression of bovine chymosin in Pichia pastoris]
    Sheng Wu Gong Cheng Xue Bao25, 1160-1165. PubMed  Europe PubMed  E
    43. 2008
  43. Galan,E., Prados,F., Pino,A., Tejada,L. and Fernandez-Salguero,J.
    Influence of different amounts of vegetable coagulant from cardoon Cynara cardunculus and calf rennet on the proteolysis and sensory characteristics of cheeses made with sheep milk
    Int Dairy J18, 93-98. DOI
  44. Vallejo,J.A., Ageitos,J.M., Poza,M. and Villa,T.G.
    Cloning and expression of buffalo active chymosin in Pichia pastoris
    J Agric Food Chem56, 10606-10610. PubMed  Europe PubMed DOI  E
    45. 2007
  45. Claverie-Martin,F. and Vega-Hernandez,M.C.
    Aspartic proteases used in cheese making
    [ISSN:978-1-4020-5376-4]207-219. DOI
  46. Reh,G., Spelzini,D., Tubio,G., Pico,G. and Farruggia,B.
    Partition features and renaturation enhancement of chymosin in aqueous two-phase systems
    J Chromatogr B Analyt Technol Biomed Life Sci860, 98-105. PubMed  Europe PubMed DOI
    47. 2006
  47. [YEAR:7-4-2006]Kappeler,S.R., van den Brink,H.J., Rahbek-Nielsen,H., Farah,Z., Puhan,Z., Hansen,E.B. and Johansen,E.
    Characterization of recombinant camel chymosin reveals superior properties for the coagulation of bovine and camel milk
    Biochem Biophys Res Commun342, 647-654. PubMed  Europe PubMed DOI
  48. [YEAR:29-6-2006]Kumar,A., Sharma,J., Mohanty,A.K., Grover,S. and Batish,V.K.
    Purification and characterization of milk clotting enzyme from goat (Capra hircus)
    Comp Biochem Physiol B Biochem Mol Biol145, 108-113. PubMed  Europe PubMed DOI
  49. Starovoitova,V.V., Velichko,T.I., Baratova,L.A., Filippova,I.Y. and Lavrenova,G.I.
    A comparative study of functional properties of calf chymosin and its recombinant forms
    Biochemistry (Mosc)71, 320-324. PubMed  Europe PubMed DOI
  50. [YEAR:16-4-2006]van den Brink,H.J., Petersen,S.G., Rahbek-Nielsen,H., Hellmuth,K. and Harboe,M.
    Increased production of chymosin by glycosylation
    J Biotechnol125, 304-310. PubMed  Europe PubMed DOI  E
    51. 2005
  51. [YEAR:15-6-2005]Callebaut,I., Schoentgen,F., Prat,K., Mornon,J.P. and Jolles,P.
    Characterization and study of a kappa-casein-like chymosin-sensitive linkage
    Biochim Biophys Acta1749, 75-80. PubMed  Europe PubMed DOI  P  I
  52. O'Mahony,J.A., Lucey,J.A. and McSweeney,P.L.
    Chymosin-mediated proteolysis, calcium solubilization, and texture development during the ripening of cheddar cheese
    J Dairy Sci88, 3101-3114. PubMed  Europe PubMed DOI
    53. 2004
  53. Foltmann,B. and Szecsi,P.B.
    Chymosin
    [ISSN:0-12-079610-4]2, 29-32.  V
  54. [YEAR:7-12-2004]Kageyama,T.
    Role of S'1 loop residues in the substrate specificities of pepsin A and chymosin
    Biochemistry43, 15122-15130. PubMed  Europe PubMed DOI  P
  55. Kim,S.Y., Gunasekaran,S. and Olson,N.F.
    Combined use of chymosin and protease from Cryphonectria parasitica for control of meltability and firmness of cheddar cheese
    J Dairy Sci87, 274-283. PubMed  Europe PubMed DOI
  56. [YEAR:19-10-2004]Vega-Hernandez,M.C., Gomez-Coello,A., Villar,J. and Claverie-Martin,F.
    Molecular cloning and expression in yeast of caprine prochymosin
    J Biotechnol114, 69-79. PubMed  Europe PubMed DOI
    57. 2003
  57. [YEAR:5-8-2003]Cardoza,R.E., Gutierrez,S., Ortega,N., Colina,A., Casqueiro,J. and Martin,J.F.
    Expression of a synthetic copy of the bovine chymosin gene in Aspergillus awamori from constitutive and pH-regulated promoters and secretion using two different pre-pro sequences
    Biotechnol Bioeng83, 249-259. PubMed  Europe PubMed DOI  K
  58. Kuhnel,B., Alcantara,J., Boothe,J., Van Rooijen,G. and Moloney,M.
    Precise and efficient cleavage of recombinant fusion proteins using mammalian aspartic proteases
    Protein Eng16, 777-783. PubMed  Europe PubMed
    59. 2002
  59. Hayes,M.G., Oliveira,J.C., McSweeney,P.L. and Kelly,A.L.
    Thermal inactivation of chymosin during cheese manufacture
    J Dairy Res69, 269-279. PubMed  Europe PubMed DOI
  60. Menzella,H.G., Gramajo,H.C. and Ceccarelli,E.A.
    High recovery of prochymosin from inclusion bodies using controlled air oxidation
    Protein Expr Purif25, 248-255. PubMed  Europe PubMed DOI  E
    61. 2001
  61. Francky,A., Francky,B.M., Strukelj,B., Gruden,K., Ritonja,A., Krizaj,I., Kregar,I., Pain,R.H. and Pungercar,J.
    A basic residue at position 36p of the propeptide is not essential for the correct folding and subsequent autocatalytic activation of prochymosin
    Eur J Biochem268, 2362-2368. PubMed  Europe PubMed DOI
  62. Mezina,M.N., Lavrenova,G.I., Prokof'ev,M.I., Starovoitova,V.V., Ermolaev,V.I., Chernyh,V.Y., Balandina,G.N. and Demidovich,S.S.
    Isolation of milk-clotting enzyme from transgenic sheep milk and its comparison with calf chymosin
    Biochemistry (Mosc)66, 378-383. PubMed  Europe PubMed DOI
    63. 2000
  63. [YEAR:10-10-2000]Chen,H., Zhang,G., Zhang,Y., Dong,Y. and Yang,K.
    Functional implications of disulfide bond, Cys206-Cys210, in recombinant prochymosin (chymosin)
    Biochemistry39, 12140-12148. PubMed  Europe PubMed DOI
  64. [YEAR:27-1-2000]Kageyama,T., Ichinose,M., Tsukada-Kato,S., Omata,M., Narita,Y., Moriyama,A. and Yonezawa,S.
    Molecular cloning of neonate/infant-specific pepsinogens from rat stomach mucosa and their expressional change during development
    Biochem Biophys Res Commun267, 806-812. PubMed  Europe PubMed DOI
  65. Kageyama,T.
    New World monkey pepsinogens A and C, and prochymosins. Purification, characterization of enzymatic properties, cDNA cloning, and molecular evolution
    J Biochem127, 761-770. PubMed  Europe PubMed
  66. Narita,Y., Oda,S.I., Takenaka,W. and Kageyama,T.
    Gastric digestive proteinases of the greater horseshoe bat, Rhinolophus ferrumequinum
    Mammal Study25, 17-26.
  67. Starovoitova,V.V., Filippova,I.Y., Lysogorskaya,E.N., Oksenoit,E.S. and Lavrenova,G.I.
    Fluorogenic substrates for assay of chymosin
    Biochemistry (Mosc)65, 713-717. PubMed  Europe PubMed  A
  68. Wei,C., Zhang,Y. and Yang,K.
    Chaperone-mediated refolding of recombinant prochymosin
    J Protein Chem19, 449-456. PubMed  Europe PubMed DOI  E
  69. Wei,C., Chen,H., Zhang,Y. and Yang,K.
    Analysis of the disulfide bonding pattern between domain sequences of recombinant prochymosin solubilized from inclusion bodies
    J Protein Chem19, 277-284. PubMed  Europe PubMed DOI  E
    70. 1999
  70. [YEAR:15-5-1999]Wei,C., Tang,B., Zhang,Y. and Yang,K.
    Oxidative refolding of recombinant prochymosin
    Biochem J340, 345-351. PubMed  Europe PubMed DOI  E
    71. 1998
  71. Albert,A., Blundell,T.L., Dhanaraj,V., Donate,L.E., Groves,M., Guruprasad,K., Nugent,P.G., Orprayoon,P., Pitts,J.E., Rufino,S., Srinivasan,N., Williams,M. and Wilsher,J.
    Protein engineering aspartic proteinases. Site-directed mutagenesis, biochemical characterisation, and X-ray analysis of chymosins with substituted single amino acid substitutions and loop replacements
    Adv Exp Med Biol436, 169-177. PubMed  Europe PubMed
  72. Chitpinityol,S., Goode,D. and Crabbe,M.J.C.
    Site-specific mutations of calf chymosin B which influence milk-clotting activity
    Food Chem62, 133-139.  E
  73. Groves,M.R., Dhanaraj,V., Badasso,M., Nugent,P., Pitts,J.E., Hoover,D.J. and Blundell,T.L.
    A 2.3 A resolution structure of chymosin complexed with a reduced bond inhibitor shows that the active site beta-hairpin flap is rearranged when compared with the native crystal structure
    Protein Eng11, 833-840. PubMed  Europe PubMed  S  I
  74. Gustchina,E.A., Majer,P., Rumsh,L.D., Ginodman,L.M. and Andreeva,N.S.
    Post X-ray crystallographic studies of chymosin specificity. The role of histidine-proline cluster of kappa-casein in catalytic reactions
    Adv Exp Med Biol436, 179-184. PubMed  Europe PubMed
  75. [YEAR:23-4-1998]Li,H., Zhang,Y., Dong,Y. and Yang,K.
    Functional implications of the 21-24 loop in recombinant prochymosin
    Biochim Biophys Acta1384, 121-129. PubMed  Europe PubMed DOI
  76. Litvinova,O.V., Balandina,G.N. and Stepanov,V.M.
    [Determination of activity of aspartic proteinases by cleavage of new chromogenic substrates]
    Bioorg Khim24, 175-178. PubMed  Europe PubMed  A
  77. [YEAR:1-11-1998]Richter,C., Tanaka,T. and Yada,R.Y.
    Mechanism of activation of the gastric aspartic proteinases: pepsinogen, progastricsin and prochymosin
    Biochem J335, 481-490. PubMed  Europe PubMed
  78. Trujillo,A.J., Miranda,G., Lebars,D. and Delacroix-Buchet,A.
    Proteolytic specificity of chymosin on caprine alphas1-caseins A and F
    J Dairy Res65, 233-241. PubMed  Europe PubMed DOI
    79. 1997
  79. Williams,M.G., Wilsher,J., Nugent,P., Mills,A., Dhanaraj,V., Fabry,M., Sedlacek,J., Uusitalo,J.M., Penttila,M.E., Pitts,J.E. and Blundell,T.L.
    Mutagenesis, biochemical characterization and X-ray structural analysis of point mutants of bovine chymosin
    Protein Eng10, 991-997. PubMed  Europe PubMed
  80. [YEAR:5-12-1997]Zhang,Y., Li,H., Wu,H., Don,Y., Liu,N. and Yang,K.
    Functional implications of disulfide bond, Cys45-Cys50, in recombinant prochymosin
    Biochim Biophys Acta1343, 278-286. PubMed  Europe PubMed DOI
    81. 1996
  81. Abdel Malak,C.A., Abou El Adab,I.F., Vukashinovic,V., Zalunin,I.A., Timokhina,E.A., Lavrenova,G.I. and Stepanov,V.M.
    Buffalo (Bos buffali L.) chymosin purification and properties
    Comp Biochem Physiol B Biochem Mol Biol113, 57-62. PubMed  Europe PubMed DOI
  82. Walsh,M.K. and Swaisgood,H.E.
    Investigating the use of the chymosin-sensitive sequence of kappa-casein as a cleavable linker site in fusion proteins
    J Biotechnol45, 235-241. PubMed  Europe PubMed DOI
  83. Yonezawa,H., Uchikoba,T., Kaneda,M. and Izumiya,N.
    Sensitive fluorometric assay for the activity of chymosin
    Int J Pept Protein Res47, 56-61. PubMed  Europe PubMed  A
    84. 1995
  84. Wang,G., Liu,N. and Yang,K.
    High-level expression of prochymosin in Escherichia coli: effect of the secondary structure of the ribosome binding site
    Protein Expr Purif6, 284-290. PubMed  Europe PubMed DOI  E
    85. 1992
  85. Foltmann,B.
    Chymosin: a short review on foetal and neonatal gastric proteases
    Scand J Clin Lab Invest Suppl210, 65-79. PubMed  Europe PubMed
  86. Malak,C.A.
    Calf chymosin as a catalyst of peptide synthesis
    Biochem J288, 941-943. PubMed  Europe PubMed
    87. 1991
  87. Newman,M., Safro,M., Frazao,C., Khan,G., Zdanov,A., Tickle,I.J., Blundell,T.L. and Andreeva,N.
    X-ray analyses of aspartic proteinases. IV. Structure and refinement at 2.2 A resolution of bovine chymosin
    J Mol Biol221, 1295-1309. PubMed  Europe PubMed  S
    88. 1990
  88. Stepanov,V.M., Lavrenova,G.I., Terent'eva,E.Yu. and Khodova,O.M.
    Prochymosin activation by non-aspartic proteinases
    FEBS Lett260, 173-175. PubMed  Europe PubMed DOI
  89. Strop,P., Sedlacek,J., Stys,J., Kaderabkova,Z., Blaha,I., Pavlickova,L., Pohl,J., Fabry,M., Kostka,V., Newman,M., Frazao,C., Shearer,A., Tickle,I.J. and Blundell,T.L.
    Engineering enzyme subsite specificity: preparation, kinetic characterization, and X-ray analysis at 2.0-A resolution of Val111Phe site-mutated calf chymosin
    Biochemistry29, 9863-9871. PubMed  Europe PubMed  S
    90. 1989
  90. Suzuki,J., Sasaki,K., Sasao,Y., Hamu,A., Kawasaki,H., Nishiyama,M., Horinouchi,S. and Beppu,T.
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