Literature for peptidase A01.006: chymosin

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

(Topics flags: S Structure, A Assay, K Knockout, P Specificity, E Expression, V Review, M Mutation, I Inhibitor. To select only the references relevant to a single topic, click the link above. See explanation.)

2025

Akishev,Z., Auyez,M., Tursunbekova,A. and Khassenov,B. Comparative biochemical properties of recombinant goat and calf chymosins and their implications in dairy processing Sci Rep (2025) 15, 26502-26502. PubMed Europe PubMed DOI PMC EPMC
Belenkaya,S.V., M G,I., Borisevich,S.S., Khamitov,E.M., Diusenova,S.E., Shevtsov,M.B., Borshchevskiy,V.I., Kolosov,P.V., Fomin,A.S., Arkhipov,S.G., Volosnikova,E.A., Elchaninov,V.V., Kolybalov,D.S. and Shcherbakov,D.N. Altai maral (Cervus elaphus sibiricus) chymosin structural characterization and modeling the enzyme interaction with the chymosin-sensitive regions of kappa-caseins Int J Biol Macromol (2025) 330, 148085-148085. PubMed Europe PubMed DOI
Zheng,Y., Wang,S., Deng,Y., Hu,P., Xue,Q., Li,J., Lei,L., Chan,Z., Yang,J. and Peng,W. Enhanced production of recombinant calf chymosin in Kluyveromyces lactis via CRISPR-Cas9 engineering Bioresour Technol (2025) 419, 132116-132116. PubMed Europe PubMed DOI

2024

Amjad,H., Saleem,F., Ahmad,M., Nisar,U. and Arshad Dar,H. Comprehensive bioinformatics-based annotation and functional characterization of bovine chymosin protein revealed novel biological insights Food Chem (Oxf) (2024) 8, 100191-100191. PubMed Europe PubMed DOI PMC EPMC

2023

Balabova,D.V., Belash,E.A., Belenkaya,S.V., Shcherbakov,D.N., Belov,A.N., Koval,A.D., Mironova,A.V., Bondar,A.A., Volosnikova,E.A., Arkhipov,S.G., Sokolova,O.O., Chirkova,V.Y. and Elchaninov,V.V. Biochemical Properties of a Promising Milk-Clotting Enzyme, Moose (Alces alces) Recombinant Chymosin Foods (2023) 12 PubMed Europe PubMed DOI PMC EPMC

2022

Wang,N., Yang,C., Peng,H., Guo,W., Wang,M., Li,G. and Liu,D. The introduction of an N-glycosylation site into prochymosin greatly enhances its production and secretion by Pichia pastoris Microb Cell Fact (2022) 21, 177-177. PubMed Europe PubMed DOI PMC EPMC

2021

Al-Zoreky,N.S. and Almathen,F.S. Using recombinant camel chymosin to make white soft cheese from camel milk Food Chem (2021) 337, 127994-127994. PubMed Europe PubMed DOI

2020

Belenkaya,S.V., Bondar,A.A., Kurgina,T.A., Elchaninov,V.V., Bakulina,A.Y., Rukhlova,E.A., Lavrik,O.I., Ilyichev,A.A. and Shcherbakov,D.N. Characterization of the Altai Maral Chymosin Gene, Production of a Chymosin Recombinant Analog in the Prokaryotic Expression System, and Analysis of Its Several Biochemical Properties Biochemistry (Mosc) (2020) 85, 781-791. PubMed Europe PubMed DOI

2019

Gumus,P. and Hayaloglu,A.A. Effects of blends of camel and calf chymosin on proteolysis, residual coagulant activity, microstructure, and sensory characteristics of Beyaz peynir J Dairy Sci (2019) 102, 5945-5956. PubMed Europe PubMed DOI
Soltani,M., Sahingil,D., Gokce,Y. and Hayaloglu,A.A. Effect of blends of camel chymosin and microbial rennet (Rhizomucor miehei) on chemical composition, proteolysis and residual coagulant activity in Iranian ultrafiltered white cheese J Food Sci Technol (2019) 56, 589-598. PubMed Europe PubMed DOI PMC EPMC

2018

Ansari,S.M., Sorensen,J., Schiott,B. and Palmer,D.S. On the effect of mutations in bovine or camel chymosin on the thermodynamics of binding kappa-caseins Proteins (2018) 86, 75-87. PubMed Europe PubMed DOI M

2017

Liu,W.G., Wang,Y.P., Zhang,Z.J., Wang,M., Lv,Q.X., Liu,H.W., Meng,L.C. and Lu,M. Generation and characterization of caprine chymosin in corn seed Protein Expr Purif (2017) 135, 78-82. PubMed Europe PubMed DOI E
Lopes-Marques,M., Ruivo,R., Fonseca,E., Teixeira,A. and Castro,L.F.C. Unusual loss of chymosin in mammalian lineages parallels neo-natal immune transfer strategies Mol Phylogenet Evol (2017) 116, 78-86. PubMed Europe PubMed DOI

2016

Ansari,S.M., Coletta,A., Kirkeby Skeby,K., Sorensen,J., Schiott,B. and Palmer,D.S. Allosteric-activation mechanism of bovine chymosin revealed by bias-exchange metadynamics and molecular dynamics simulations J Phys Chem B (2016) 120, 10453-10462. PubMed Europe PubMed DOI
Espinoza-Molina,J.A., Acosta-Muniz,C.H., Sepulveda,D.R., Zamudio-Flores,P.B. and Rios-Velasco,C. Codon optimization of the "Bos taurus chymosin" gene for the production of recombinant chymosin in Pichia pastoris Mol Biotechnol (2016) 58, 657-664. PubMed Europe PubMed DOI E
Huang,J., Zhu,Y., Sun,B., Yao,Y. and Liu,J. Determination of the protonation state of the Asp dyad: conventional molecular dynamics versus thermodynamic integration J Mol Model (2016) 22, 58-58. PubMed Europe PubMed DOI
Luo,F., Jiang,W.H., Yang,Y.X., Li,J. and Jiang,M.F. Cloning and Expression of Yak Active Chymosin in Pichia pastoris Asian-Australas J Anim Sci (2016) 29, 1363-1370. PubMed Europe PubMed DOI E
Noseda,D.G., Recupero,M., Blasco,M., Bozzo,J. and Galvagno,M.A. Production in stirred-tank bioreactor of recombinant bovine chymosin B by a high-level expression transformant clone of Pichia pastoris Protein Expr Purif (2016) 123, 112-121. PubMed Europe PubMed DOI E
Rengifo,A.F.C., Ferreira,G.M.D., Ferreira,G.M.D., daSilva,M.C.H., dePaula Rezende,J., dosSantos Pires,A.C. and daSilva,L.H.M. Driving forces for chymosin partitioning on the macromolecule-salt aqueous two phase system Food Bioprod Process (2016) 100, 361-371. DOI
Tyagi,A., Kumar,A., Yadav,A.K., ChandolaSaklani,A., Grover,S. and Batish,V.K. Functional expression of recombinant goat chymosin in Pichia pastoris bioreactor cultures: a commercially viable alternate LWT - Food Sci Technol (2016) 69, 217-224. DOI E
Ulusu,Y., Senturk,S.B., Kudug,H. and Gokce,I. Expression, purification, and characterization of bovine chymosin enzyme using an inducible pTOLT system Prep Biochem Biotechnol (2016) 46, 596-601. PubMed Europe PubMed DOI E
Wei,Z.-Y., Zhang,Y.-Y., Wang,Y.-P., Fan,M.-X., Zhong,X.-F., Xu,N., Lin,F. and Xing,S.-C. Production of bioactive recombinant bovine chymosin in tobacco plants Int J Mol Sci (2016) 17 PubMed Europe PubMed DOI E

2015

Jensen,J.L., Jacobsen,J., Moss,M.L., Rasmussen,F., Qvist,K.B., Larsen,S. and van den Brink,J.M. The function of the milk-clotting enzymes bovine and camel chymosin studied by a fluorescence resonance energy transfer assay J Dairy Sci (2015) 98, 2853-2860. PubMed Europe PubMed DOI A
Rolet-Repecaud,O., Arnould,C., Dupont,D., Gavoye,S., Beuvier,E. and Achilleos,C. Development and evaluation of a monoclonal antibody-based inhibition ELISA for the quantification of chymosin in solution J Agric Food Chem (2015) 63, 4799-4804. PubMed Europe PubMed DOI
Wang,N., Wang,K.Y., Li,G., Guo,W. and Liu,D. Expression and characterization of camel chymosin in Pichia pastoris Protein Expr Purif (2015) 111, 75-81. PubMed Europe PubMed DOI E

2014

Noseda,D.G., Blasco,M., Recupero,M. and Galvagno,M.A. Bioprocess and downstream optimization of recombinant bovine chymosin B in Pichia (Komagataella) pastoris under methanol-inducible AOXI promoter Protein Expr Purif (2014) 104C, 85-91. PubMed Europe PubMed DOI E

2013

Langholm Jensen,J., Molgaard,A., Navarro Poulsen,J.C., Harboe,M.K., Simonsen,J.B., Lorentzen,A.M., Hjerno,K., van den Brink,J.M., Qvist,K.B. and Larsen,S. Camel and bovine chymosin: the relationship between their structures and cheese-making properties Acta Crystallogr D Biol Crystallogr (2013) 69, 901-913. PubMed Europe PubMed DOI PMC EPMC S
Noseda,D.G., Recupero,M.N., Blasco,M., Ortiz,G.E. and Galvagno,M.A. Cloning, expression and optimized production in a bioreactor of bovine chymosin B in Pichia (Komagataella) pastoris under AOX1 promoter Protein Expr Purif (2013) 92, 235-244. PubMed Europe PubMed DOI E
Yoon,J., Kikuma,T., Maruyama,J. and Kitamoto,K. Enhanced production of bovine chymosin by autophagy deficiency in the filamentous fungus Aspergillus oryzae PLoS ONE (2013) 8, e62512-e62512. PubMed Europe PubMed DOI PMC EPMC E

2012

Garcia,V., Rovira,S., Teruel,R., Boutoial,K., Rodriguez,J., Roa,I. and Lopez,M.B. Effect of vegetable coagulant, microbial coagulant and calf rennet on physicochemical, proteolysis, sensory and texture profiles of fresh goats cheese Dairy Sci Technol (2012) 92, 691-707. DOI
Hsieh,J.F. and Pan,P.H. Proteomic profiling of the coagulation of milk proteins induced by chymosin J Agric Food Chem (2012) 60, 2039-2045. PubMed Europe PubMed DOI
Moller,K.K., Rattray,F.P., Sorensen,J.C. and Ardo,Y. Comparison of the hydrolysis of bovine kappa-casein by camel and bovine chymosin: a kinetic and specificity study J Agric Food Chem (2012) 60, 5454-5460. PubMed Europe PubMed DOI
Moller,K.K., Rattray,F.P. and Ardo,Y. Camel and bovine chymosin hydrolysis of bovine alpha(S1)- and beta-caseins studied by comparative peptide mapping J Agric Food Chem (2012) 60, 11421-11432. PubMed Europe PubMed DOI P
Szecsi,P.B. and Harboe,M. Chymosin [ISSN:978-0-12-407744-7] (2012) 3, 37-42. DOI

2011

Barac,M., Cabrilo,S., Pesic,M., Stanojevic,S., Pavlicevic,M., Macej,O. and Ristic,N. Functional properties of pea (Pisum sativum, L.) protein isolates modified with chymosin Int J Mol Sci (2011) 12, 8372-8387. PubMed Europe PubMed DOI PMC EPMC
Heinrich,M. and Kulozik,U. Study of chymosin hydrolysis of casein micelles under ultra high pressure: effect on re-association upon pressure release Int Dairy J (2011) 21, 664-669. DOI
Jacob,M., Jaros,D. and Rohm,H. Proteolytic activity of traditional calf rennet and selected milk coagulant substitutes Milchwissenschaft (2011) 66, 240-244.
Sorensen,J., Palmer,D.S., Qvist,K.B. and Schiott,B. Initial stage of cheese production: a molecular modeling study of bovine and camel chymosin complexed with peptides from the chymosin-sensitive region of kappa-casein J Agric Food Chem (2011) 59, 5636-5647. PubMed Europe PubMed DOI

2010

Kageyama,H., Ueda,H., Tezuka,T., Ogasawara,A., Narita,Y., Kageyama,T. and Ichinose,M. Differences in the P1' substrate specificities of pepsin A and chymosin J Biochem (2010) 147, 167-174. PubMed Europe PubMed DOI P
Kumar,A., Grover,S., Sharma,J. and Batish,V.K. Chymosin and other milk coagulants: sources and biotechnological interventions Crit Rev Biotechnol (2010) 30, 243-258. PubMed Europe PubMed DOI V
Palmer,D.S., Christensen,A.U., Sorensen,J., Celik,L., Qvist,K.B. and Schiott,B. Bovine chymosin: a computational study of recognition and binding of bovine kappa-casein Biochemistry (2010) 49, 2563-2573. PubMed Europe PubMed DOI
Yuan,W., Ke,T., Du,M., Chu,X., Hu,F. and Hui,F. [Gene synthesis of the bovine prochymosin gene and high-level expression in Kluyvermyces lactis] Sheng Wu Gong Cheng Xue Bao (2010) 26, 1281-1286. PubMed Europe PubMed E

2009

Bansal,N., Drake,M.A., Piraino,P., Broe,M.L., Harboe,M., Fox,P.F. and McSweeney,P.L.H. Suitability of recombinant camel (Camelus dromedarius) chymosin as a coagulant for Cheddar cheese Int Dairy J (2009) 19, 510-517. DOI
Zhang,L., Jiang,Y., Zhang,J. and Yang,Z. [Recombinant expression of bovine chymosin in Pichia pastoris] Sheng Wu Gong Cheng Xue Bao (2009) 25, 1160-1165. PubMed Europe PubMed E

2008

Galan,E., Prados,F., Pino,A., Tejada,L. and Fernandez-Salguero,J. Influence of different amounts of vegetable coagulant from cardoon Cynara cardunculus and calf rennet on the proteolysis and sensory characteristics of cheeses made with sheep milk Int Dairy J (2008) 18, 93-98. DOI
Vallejo,J.A., Ageitos,J.M., Poza,M. and Villa,T.G. Cloning and expression of buffalo active chymosin in Pichia pastoris J Agric Food Chem (2008) 56, 10606-10610. PubMed Europe PubMed DOI E

2007

Claverie-Martin,F. and Vega-Hernandez,M.C. Aspartic proteases used in cheese making [ISSN:978-1-4020-5376-4] (2007) , 207-219. DOI
Reh,G., Spelzini,D., Tubio,G., Pico,G. and Farruggia,B. Partition features and renaturation enhancement of chymosin in aqueous two-phase systems J Chromatogr B Analyt Technol Biomed Life Sci (2007) 860, 98-105. PubMed Europe PubMed DOI

2006

[YEAR:7-4-2006]Kappeler,S.R., van den Brink,H.J., Rahbek-Nielsen,H., Farah,Z., Puhan,Z., Hansen,E.B. and Johansen,E. Characterization of recombinant camel chymosin reveals superior properties for the coagulation of bovine and camel milk Biochem Biophys Res Commun (7-4-2006) 342, 647-654. PubMed Europe PubMed DOI
[YEAR:29-6-2006]Kumar,A., Sharma,J., Mohanty,A.K., Grover,S. and Batish,V.K. Purification and characterization of milk clotting enzyme from goat (Capra hircus) Comp Biochem Physiol B Biochem Mol Biol (29-6-2006) 145, 108-113. PubMed Europe PubMed DOI
Starovoitova,V.V., Velichko,T.I., Baratova,L.A., Filippova,I.Y. and Lavrenova,G.I. A comparative study of functional properties of calf chymosin and its recombinant forms Biochemistry (Mosc) (2006) 71, 320-324. PubMed Europe PubMed DOI
[YEAR:16-4-2006]van den Brink,H.J., Petersen,S.G., Rahbek-Nielsen,H., Hellmuth,K. and Harboe,M. Increased production of chymosin by glycosylation J Biotechnol (16-4-2006) 125, 304-310. PubMed Europe PubMed DOI E

2005

[YEAR:15-6-2005]Callebaut,I., Schoentgen,F., Prat,K., Mornon,J.P. and Jolles,P. Characterization and study of a kappa-casein-like chymosin-sensitive linkage Biochim Biophys Acta (15-6-2005) 1749, 75-80. PubMed Europe PubMed DOI P I
O'Mahony,J.A., Lucey,J.A. and McSweeney,P.L. Chymosin-mediated proteolysis, calcium solubilization, and texture development during the ripening of cheddar cheese J Dairy Sci (2005) 88, 3101-3114. PubMed Europe PubMed DOI

2004

Foltmann,B. and Szecsi,P.B. Chymosin [ISSN:0-12-079610-4] (2004) 2, 29-32. V
[YEAR:7-12-2004]Kageyama,T. Role of S'1 loop residues in the substrate specificities of pepsin A and chymosin Biochemistry (7-12-2004) 43, 15122-15130. PubMed Europe PubMed DOI P
Kim,S.Y., Gunasekaran,S. and Olson,N.F. Combined use of chymosin and protease from Cryphonectria parasitica for control of meltability and firmness of cheddar cheese J Dairy Sci (2004) 87, 274-283. PubMed Europe PubMed DOI
[YEAR:19-10-2004]Vega-Hernandez,M.C., Gomez-Coello,A., Villar,J. and Claverie-Martin,F. Molecular cloning and expression in yeast of caprine prochymosin J Biotechnol (19-10-2004) 114, 69-79. PubMed Europe PubMed DOI

2003

[YEAR:5-8-2003]Cardoza,R.E., Gutierrez,S., Ortega,N., Colina,A., Casqueiro,J. and Martin,J.F. Expression of a synthetic copy of the bovine chymosin gene in Aspergillus awamori from constitutive and pH-regulated promoters and secretion using two different pre-pro sequences Biotechnol Bioeng (5-8-2003) 83, 249-259. PubMed Europe PubMed DOI K
Kuhnel,B., Alcantara,J., Boothe,J., Van Rooijen,G. and Moloney,M. Precise and efficient cleavage of recombinant fusion proteins using mammalian aspartic proteases Protein Eng (2003) 16, 777-783. PubMed Europe PubMed

2002

Hayes,M.G., Oliveira,J.C., McSweeney,P.L. and Kelly,A.L. Thermal inactivation of chymosin during cheese manufacture J Dairy Res (2002) 69, 269-279. PubMed Europe PubMed DOI
Menzella,H.G., Gramajo,H.C. and Ceccarelli,E.A. High recovery of prochymosin from inclusion bodies using controlled air oxidation Protein Expr Purif (2002) 25, 248-255. PubMed Europe PubMed DOI E

2001

Francky,A., Francky,B.M., Strukelj,B., Gruden,K., Ritonja,A., Krizaj,I., Kregar,I., Pain,R.H. and Pungercar,J. A basic residue at position 36p of the propeptide is not essential for the correct folding and subsequent autocatalytic activation of prochymosin Eur J Biochem (2001) 268, 2362-2368. PubMed Europe PubMed DOI
Mezina,M.N., Lavrenova,G.I., Prokof'ev,M.I., Starovoitova,V.V., Ermolaev,V.I., Chernyh,V.Y., Balandina,G.N. and Demidovich,S.S. Isolation of milk-clotting enzyme from transgenic sheep milk and its comparison with calf chymosin Biochemistry (Mosc) (2001) 66, 378-383. PubMed Europe PubMed DOI

2000

[YEAR:10-10-2000]Chen,H., Zhang,G., Zhang,Y., Dong,Y. and Yang,K. Functional implications of disulfide bond, Cys206-Cys210, in recombinant prochymosin (chymosin) Biochemistry (10-10-2000) 39, 12140-12148. PubMed Europe PubMed DOI
Kageyama,T. New World monkey pepsinogens A and C, and prochymosins. Purification, characterization of enzymatic properties, cDNA cloning, and molecular evolution J Biochem (2000) 127, 761-770. PubMed Europe PubMed
[YEAR:27-1-2000]Kageyama,T., Ichinose,M., Tsukada-Kato,S., Omata,M., Narita,Y., Moriyama,A. and Yonezawa,S. Molecular cloning of neonate/infant-specific pepsinogens from rat stomach mucosa and their expressional change during development Biochem Biophys Res Commun (27-1-2000) 267, 806-812. PubMed Europe PubMed DOI
Narita,Y., Oda,S.I., Takenaka,W. and Kageyama,T. Gastric digestive proteinases of the greater horseshoe bat, Rhinolophus ferrumequinum Mammal Study (2000) 25, 17-26.
Starovoitova,V.V., Filippova,I.Y., Lysogorskaya,E.N., Oksenoit,E.S. and Lavrenova,G.I. Fluorogenic substrates for assay of chymosin Biochemistry (Mosc) (2000) 65, 713-717. PubMed Europe PubMed A
Wei,C., Chen,H., Zhang,Y. and Yang,K. Analysis of the disulfide bonding pattern between domain sequences of recombinant prochymosin solubilized from inclusion bodies J Protein Chem (2000) 19, 277-284. PubMed Europe PubMed DOI E
Wei,C., Zhang,Y. and Yang,K. Chaperone-mediated refolding of recombinant prochymosin J Protein Chem (2000) 19, 449-456. PubMed Europe PubMed DOI E

1999

[YEAR:15-5-1999]Wei,C., Tang,B., Zhang,Y. and Yang,K. Oxidative refolding of recombinant prochymosin Biochem J (15-5-1999) 340, 345-351. PubMed Europe PubMed DOI PMC EPMC E

1998

Albert,A., Blundell,T.L., Dhanaraj,V., Donate,L.E., Groves,M., Guruprasad,K., Nugent,P.G., Orprayoon,P., Pitts,J.E., Rufino,S., Srinivasan,N., Williams,M. and Wilsher,J. Protein engineering aspartic proteinases. Site-directed mutagenesis, biochemical characterisation, and X-ray analysis of chymosins with substituted single amino acid substitutions and loop replacements Adv Exp Med Biol (1998) 436, 169-177. PubMed Europe PubMed
Chitpinityol,S., Goode,D. and Crabbe,M.J.C. Site-specific mutations of calf chymosin B which influence milk-clotting activity Food Chem (1998) 62, 133-139. E
Groves,M.R., Dhanaraj,V., Badasso,M., Nugent,P., Pitts,J.E., Hoover,D.J. and Blundell,T.L. A 2.3 A resolution structure of chymosin complexed with a reduced bond inhibitor shows that the active site beta-hairpin flap is rearranged when compared with the native crystal structure Protein Eng (1998) 11, 833-840. PubMed Europe PubMed S I
Gustchina,E.A., Majer,P., Rumsh,L.D., Ginodman,L.M. and Andreeva,N.S. Post X-ray crystallographic studies of chymosin specificity. The role of histidine-proline cluster of kappa-casein in catalytic reactions Adv Exp Med Biol (1998) 436, 179-184. PubMed Europe PubMed
[YEAR:23-4-1998]Li,H., Zhang,Y., Dong,Y. and Yang,K. Functional implications of the 21-24 loop in recombinant prochymosin Biochim Biophys Acta (23-4-1998) 1384, 121-129. PubMed Europe PubMed DOI
Litvinova,O.V., Balandina,G.N. and Stepanov,V.M. [Determination of activity of aspartic proteinases by cleavage of new chromogenic substrates] Bioorg Khim (1998) 24, 175-178. PubMed Europe PubMed A
[YEAR:1-11-1998]Richter,C., Tanaka,T. and Yada,R.Y. Mechanism of activation of the gastric aspartic proteinases: pepsinogen, progastricsin and prochymosin Biochem J (1-11-1998) 335, 481-490. PubMed Europe PubMed PMC EPMC
Trujillo,A.J., Miranda,G., Lebars,D. and Delacroix-Buchet,A. Proteolytic specificity of chymosin on caprine alphas1-caseins A and F J Dairy Res (1998) 65, 233-241. PubMed Europe PubMed DOI

1997

Williams,M.G., Wilsher,J., Nugent,P., Mills,A., Dhanaraj,V., Fabry,M., Sedlacek,J., Uusitalo,J.M., Penttila,M.E., Pitts,J.E. and Blundell,T.L. Mutagenesis, biochemical characterization and X-ray structural analysis of point mutants of bovine chymosin Protein Eng (1997) 10, 991-997. PubMed Europe PubMed
[YEAR:5-12-1997]Zhang,Y., Li,H., Wu,H., Don,Y., Liu,N. and Yang,K. Functional implications of disulfide bond, Cys45-Cys50, in recombinant prochymosin Biochim Biophys Acta (5-12-1997) 1343, 278-286. PubMed Europe PubMed DOI

1996

Abdel Malak,C.A., Abou El Adab,I.F., Vukashinovic,V., Zalunin,I.A., Timokhina,E.A., Lavrenova,G.I. and Stepanov,V.M. Buffalo (Bos buffali L.) chymosin purification and properties Comp Biochem Physiol B Biochem Mol Biol (1996) 113, 57-62. PubMed Europe PubMed DOI
Walsh,M.K. and Swaisgood,H.E. Investigating the use of the chymosin-sensitive sequence of kappa-casein as a cleavable linker site in fusion proteins J Biotechnol (1996) 45, 235-241. PubMed Europe PubMed DOI
Yonezawa,H., Uchikoba,T., Kaneda,M. and Izumiya,N. Sensitive fluorometric assay for the activity of chymosin Int J Pept Protein Res (1996) 47, 56-61. PubMed Europe PubMed A

1995

Wang,G., Liu,N. and Yang,K. High-level expression of prochymosin in Escherichia coli: effect of the secondary structure of the ribosome binding site Protein Expr Purif (1995) 6, 284-290. PubMed Europe PubMed DOI E

1992

Foltmann,B. Chymosin: a short review on foetal and neonatal gastric proteases Scand J Clin Lab Invest Suppl (1992) 210, 65-79. PubMed Europe PubMed
Malak,C.A. Calf chymosin as a catalyst of peptide synthesis Biochem J (1992) 288, 941-943. PubMed Europe PubMed PMC EPMC

1991

Newman,M., Safro,M., Frazao,C., Khan,G., Zdanov,A., Tickle,I.J., Blundell,T.L. and Andreeva,N. X-ray analyses of aspartic proteinases. IV. Structure and refinement at 2.2 A resolution of bovine chymosin J Mol Biol (1991) 221, 1295-1309. PubMed Europe PubMed S

1990

Stepanov,V.M., Lavrenova,G.I., Terent'eva,E.Yu. and Khodova,O.M. Prochymosin activation by non-aspartic proteinases FEBS Lett (1990) 260, 173-175. PubMed Europe PubMed DOI
Strop,P., Sedlacek,J., Stys,J., Kaderabkova,Z., Blaha,I., Pavlickova,L., Pohl,J., Fabry,M., Kostka,V., Newman,M., Frazao,C., Shearer,A., Tickle,I.J. and Blundell,T.L. Engineering enzyme subsite specificity: preparation, kinetic characterization, and X-ray analysis at 2.0-A resolution of Val111Phe site-mutated calf chymosin Biochemistry (1990) 29, 9863-9871. PubMed Europe PubMed S

1989

Suzuki,J., Sasaki,K., Sasao,Y., Hamu,A., Kawasaki,H., Nishiyama,M., Horinouchi,S. and Beppu,T. Alteration of catalytic properties of chymosin by site-directed mutagenesis Protein Eng (1989) 2, 563-569. PubMed Europe PubMed

1988

[YEAR:15-7-1988]Danley,D.E. and Geoghegan,K.F. Structure and mechanism of formation of recombinant-derived chymosin C J Biol Chem (15-7-1988) 263, 9785-9789. PubMed Europe PubMed E

1987

Dunn,B.M., Valler,M.J., Rolph,C.E., Foundling,S.I., Jimenez,M. and Kay,J. The pH dependence of the hydrolysis of chromogenic substrates of the type, Lys-Pro-Xaa-Yaa-Phe-(NO2)Phe-Arg-Leu, by selected aspartic proteinases: evidence for specific interactions in subsites S3 and S2 Biochim Biophys Acta (1987) 913, 122-130. PubMed Europe PubMed
Kawaguchi,Y., Kosugi,S., Sasaki,K., Uozumi,T. and Beppu,T. Production of chymosin in Escherichia coli cells and its enzymatic properties Agric Biol Chem (1987) 51, 1871-1877. DOI E
Visser,S., Slangen,C.J. and van Rooijen,P.J. Peptide substrates for chymosin (rennin). Interaction sites in kappa-casein-related sequences located outside the (103-108)- hexapeptide region that fits into the enzyme's active-site cleft Biochem J (1987) 244, 553-558. PubMed Europe PubMed PMC EPMC

1985

McCaman,M.T., Andrews,W.H. and Files,J.G. Enzymatic properties and processing of bovine prochymosin synthesized in Escherichia coli J Biotechnol (1985) 2, 177-190. E

1982

Moir,D., Mao,J., Schumm,J.W., Vovis,G.F., Alford,B.L. and Taunton-Rigby,A. Molecular cloning and characterization of double-stranded cDNA coding for bovine chymosin Gene (1982) 19, 127-138. PubMed Europe PubMed DOI

1981

Foltmann,B. Gastric proteinases - structure, function, evolution and mechanism of action Essays Biochem (1981) 17, 52-84. PubMed Europe PubMed

1980

[YEAR:10-7-1980]Nakatani,H., Tsuchiya,M., Morita,T., Ohki,S. and Hiromi,K. Binding of Streptomyces pepsin inhibitor (acetyl-pepstatin) with chymosin (rennin) Biochim Biophys Acta (10-7-1980) 614, 144-150. PubMed Europe PubMed I
Visser,S., van Rooijen,P.J. and Slangen,C.J. Peptide substrates for chymosin (rennin). Isolation and substrate behaviour of two tryptic fragments of bovine kappa casein Eur J Biochem (1980) 108, 415-421. PubMed Europe PubMed

1979

Mulvihill,O.M. and Fox,P.F. Proteolytic specificity of chymosin on bovine s1-casein J Dairy Res (1979) 46, 641-651.
Mulvihill,O.M. and Fox,P.F. Proteolytic specificity of chymosins and pepsins on beta caseins Milchwissenschaft (1979) 34, 680-683.
Pedersen,V.B., Christensen,K.A. and Foltmann,B. Investigations on the activation of bovine prochymosin Eur J Biochem (1979) 94, 573-580. PubMed Europe PubMed

1978

Yamauchi,K. and Yoneda,Y. Effect of dephosphorylation of casein on its coagulation and proteolysis by chymosin Agric Biol Chem (1978) 42, 1031-1035. DOI

1977

Schattenkerk,C. Studies on polypeptides XXII: a synthetic hexapeptide as a standard substrate for chymosin (rennin) Recueil des Travaux Chimiques des Pays-Bas (1977) 96, 235-237. DOI A

1973

Kamaryt,J. and Mechlova,V. The chymosin activity of the gastric and duodenal juice proteinases separated by means of agar gel electrophoresis Clin Chem Lab Med (1973) 11, 398-402. DOI

1972

Raymond,M.N., Garnier,J., Bricas,E., Cilianu,S., Blasnic,M., Chaix,A. and Lefrancier,P. Studies on the specificity of chymosin (rennin). I. Kinetic parameters of the hydrolysis of synthetic oligopeptide substrates Biochimie (1972) 54, 145-154. PubMed Europe PubMed P

1971

Voynick,I.M. and Fruton,J.S. The comparative specificity of acid proteinases Proc Natl Acad Sci U S A (1971) 68, 257-259. PubMed Europe PubMed PMC EPMC P

1969

Lawrence,R.C. and Creamer,L.K. Action of calf rennet and other proteolytic enzymes on -casein J Dairy Res (1969) 36, 11-20.

1966

Foltmann,B. A review on prorennin and rennin C R Trav Lab Carlsberg (1966) 35, 143-231. PubMed Europe PubMed

1965

Mocquot,G. and Garnier,J. Proteolytic activity of crystalline rennin and caseins associations J Agric Food Chem (1965) 13, 414-418.

1945

Berridge,N.J. The purification and crystallization of rennin Biochem J (1945) 39, 179-186. PubMed Europe PubMed

1934

Tauber,H. and Kleiner,I.S. The chemical nature of rennin J Biol Chem (1934) 104, 259-266.

1932

Pepsin and rennin JAMA (1932) 99, 835-836. DOI

1923

Hammarsten,O. Studien Uber Chymosin- und Pepsinwirkung. IX. Mitteilung. Uber das Verschiedene verhalten der Magenenzyme von Kalb und Schwein gegen Saurewirkung beim Erwarmen HOPPE-SEYLERS'S Z PHYSIOL CHEM (1923) 130, 55-71. DOI

1918

Hammarsten,O. Studien uber Chymosin- und Pepsinwirkung: IV. Mitteilung. Die Wirkung der Enzyme auf Natriumcaseinate HOPPE-SEYLERS'S Z PHYSIOL CHEM (1918) 102, 33-77. DOI
Hammarsten,O. Studien uber Chymosin- und Pepsinwirkung: V. Mitteilung. Wirkung der Enzyme auf Erbsenlegumine HOPPE-SEYLERS'S Z PHYSIOL CHEM (1918) 102, 105-147. DOI

1914

Are pepsin and rennin identical? JAMA (1914) LXII, 855-856. DOI

1911

Porter,A.E. On the question of the identity of pepsin and rennet J Physiol (1911) 42, 389-401. PubMed Europe PubMed DOI

1905

Sawjalow,W. Zur Frage nach der Identitat von Pepsin und Chymosin HOPPE-SEYLERS'S Z PHYSIOL CHEM (1905) 46, 307-331. DOI