Substrates for peptidase M34.001: anthrax lethal factor

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Pharma

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	Evidence	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference	CutDB
dual specificity mitogen-activated protein kinase kinase 1	Q02750	2-393	peptide-Pro8+Ile-peptide	N		Lys	Pro	Thr	Pro	Ile	Gln	Leu	Asn	Vitale et al., 2000	17103
dual specificity mitogen-activated protein kinase kinase 2	P36507	1-400	peptide-Pro10+Ala-peptide	N		Pro	Val	Leu	Pro	Ala	Leu	Thr	Ile	Vitale et al., 2000	17105
Dual specificity mitogen-activated protein kinase kinase 7	O14733	1-419	peptide-Gln44+Leu-peptide	N		Pro	Thr	Leu	Gln	Leu	Pro	Leu	Ala	Vitale et al., 2000	17109
Dual specificity mitogen-activated protein kinase kinase 7	O14733	1-419	peptide-Glu76+Leu-peptide	N		His	Met	Leu	Gly	Leu	Pro	Ser	Thr	Vitale et al., 2000	17110
Mca-Lys-Lys-Pro-Thr-Pro-Ile-Gln-Leu-Asn-Dnp			Mca-Lys-Lys-Pro-Thr-Pro+Ile-Gln-Leu-Asn-Dnp	S		Lys	Pro	Thr	Pro	Ile	Gln	Leu	Asn	Li et al., 2011
Mca-Lys-Lys-Trp-Leu-Met-Tyr-Pro-Leu-Glu-Lys-Dnp			Mca-Lys-Lys-Trp-Leu-Met+Tyr-Pro-Leu-Glu-Lys-Dnp	S		Lys	Trp	Leu	Met	Tyr	Pro	Leu	Glu	Li et al., 2011
Mca-Lys-Lys-Val-Tyr-Pro-Tyr-Pro-Met-Glu-Lys-Dnp			Mca-Lys-Lys-Val-Tyr-Pro+Tyr-Pro-Met-Glu-Lys-Dnp	S		Lys	Val	Tyr	Pro	Tyr	Pro	Met	Glu	Li et al., 2011
mitogen-activated protein kinase kinase 1	Q05116	2-395	peptide-Pro8+Thr-peptide	N		Lys	Pro	Thr	Pro	Ile	Gln	Leu	Asn	Duesbery et al., 1998
mitogen-activated protein kinase kinase MKK3b	P46734	1-347	peptide-Arg26+Ile-peptide	N		Lys	Asp	Leu	Arg	Ile	Ser	Cys	Met	Vitale et al., 2000	17106
mitogen-activated protein kinase kinase MKK4	P45985	1-399	peptide-Lys45+Leu-peptide	N		Lys	Ala	Leu	Lys	Leu	Asn	Phe	Ala	Vitale et al., 2000	17107
mitogen-activated protein kinase kinase MKK4	P45985	1-399	peptide-Arg58+Phe-peptide	N		Ser	Thr	Ala	Arg	Phe	Thr	Leu	Asn	Vitale et al., 2001	19465
mitogen-activated protein kinase kinase MKK6b	P52564	1-334	peptide-Arg14+Ile-peptide	N		Pro	Gly	Leu	Lys	Ile	Pro	Lys	Glu	Vitale et al., 2000	17108
NACHT, LRR and PYD domains-containing protein 1b allele 1	Q2LKW6	1-1233	peptide-Lys38+Leu-peptide	P	NT	His	Arg	Pro	Lys	Leu	Glu	Arg	His	Mendenhall et al., 2020
NACHT, LRR and PYD domains-containing protein 1b allele 1	Q2LKW6	1-1233	peptide-Lys44+Leu-peptide	P	NT	Arg	His	Leu	Lys	Leu	Gly	Met	Ile	Mendenhall et al., 2020
nLR family, pyrin domain-containing 1A	D9I2F9	1-1218	peptide-Pro44+Leu-peptide	P	NT	Arg	Pro	Arg	Pro	Leu	Pro	Arg	Val	Mendenhall et al., 2020
phosphatidylinositol 3-kinase regulatory subunit alpha	P27986	2-724	peptide-Pro94+Leu-peptide	P	NT	Pro	Pro	Arg	Pro	Leu	Pro	Val	Ala	Mendenhall et al., 2020
phosphatidylinositol 3-kinase regulatory subunit beta	O00459	1-728	peptide-Pro95+Leu-peptide	P	NT	Gly	Pro	Arg	Pro	Leu	Pro	Ala	Arg	Mendenhall et al., 2020