Substrates for peptidase M16.001: pitrilysin

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference
atrial natriuretic factor	P01160	124-151	Ser-Leu+Arg-peptide	N	-	-	Ser	Leu	Arg	Arg	Ser	Ser	Cornista et al., 2004
atrial natriuretic factor	P01160	124-151	peptide-Arg14+Ile-peptide	N	Arg	Met	Asp	Arg	Ile	Gly	Ala	Gln	Cornista et al., 2004
atrial natriuretic factor	P01160	124-151	peptide-Gly22+Cys-peptide	N	Ser	Gly	Leu	Gly	Cys	Asn	Ser	Phe	Cornista et al., 2004
beta-amyloid			peptide-His14+Gln-peptide	N	Glu	Val	His	His	Gln	Lys	Leu	Val	Cornista et al., 2004
beta-endorphin	P01189	237-267	peptide-Lys19+Asn-peptide	N	Thr	Leu	Phe	Lys	Asn	Ala	Ile	Ile	Cornista et al., 2004
beta-endorphin	P01189	237-267	peptide-Phe18+Lys-peptide	N	Val	Thr	Leu	Phe	Lys	Asn	Ala	Ile	Cornista et al., 2004
calcitonin	P01258	85-116	peptide-His20+Thr-peptide	N	Asn	Lys	Phe	His	Thr	Phe	Pro	Gln	Cornista et al., 2004
corticotropin	P01189	138-176	Ser-Tyr+Ser-peptide	N	-	-	Ser	Tyr	Ser	Met	Glu	His	Cornista et al., 2004
corticotropin	P01189	138-176	peptide-Lys15+Lys-peptide	N	Pro	Val	Gly	Lys	Lys	Arg	Arg	Pro	Cornista et al., 2004
glucagon	P01274	53-81	His-Ser-Gln-Gly-Thr-Phe+Thr-Ser-Asp-Tyr-Ser-Lys-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr	N	Gln	Gly	Thr	Phe	Thr	Ser	Asp	Tyr	Cornista et al., 2004
glucagon	P01274	53-81	His-Ser-Gln-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Lys+Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr	N	Ala	Tyr	Ser	Lys	Tyr	Leu	Asp	Ser	Cornista et al., 2004
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr+Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N	Glu	Ala	Leu	Tyr	Leu	Val	Cya	Gly	Roth, 2004
leumorphin	P01214	228-256	peptide-Arg233+Arg-peptide	N	Gly	Phe	Leu	Arg	Arg	Gln	Phe	Lys	Cornista et al., 2004
leumorphin	P01214	228-256	peptide-Thr13+Arg-peptide	N	Lys	Val	Val	Thr	Arg	Ser	Gln	Glu	Cornista et al., 2004
QF-27			Mca-Nle-Ala-Val-Lys-Lys-Tyr+Leu-Asn-Ser-Lys(Dnp)-Leu-Asp-D-Lys	S	Val	Lys	Lys	Tyr	Leu	Asn	Ser	Lys	Roth, 2004
somatostatin-28	P61278	89-116	peptide-Phe17+Phe-peptide	N	Cys	Lys	Asn	Phe	Phe	Trp	Lys	Thr	Cornista et al., 2004
somatostatin-28	P61278	89-116	peptide-Trp19+Lys-peptide	N	Asn	Phe	Phe	Trp	Lys	Thr	Phe	Thr	Cornista et al., 2004
substance P	P20366	58-68	Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly+Leu-Met-NH2	N	Gln	Phe	Phe	Gly	Leu	Met	-	-	Cornista et al., 2004
substance P	P20366	58-68	Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu+Met-NH2	N	Phe	Phe	Gly	Leu	Met	-	-	-	Cornista et al., 2004
vasoactive intestinal peptide 10-28			Tyr-Thr-Arg-Leu+Arg-Lys-Gln-Met-Ala-Val-Lys-Lys-Tyr-Leu-Asn-Ser-Ile-Leu-Asn-NH2	N	Tyr	Thr	Arg	Leu	Arg	Lys	Gln	Met	Roth, 2004
vasoactive intestinal peptide 10-28			Tyr-Thr-Arg-Leu-Arg-Lys-Gln-Met-Ala-Val-Lys-Lys-Tyr-Leu+Asn-Ser-Ile-Leu-Asn-NH2	N	Lys	Lys	Tyr	Leu	Asn	Ser	Ile	Leu	Roth, 2004
vasoactive intestinal peptide 10-28			Tyr-Thr-Arg-Leu-Arg+Lys-Gln-Met-Ala-Val-Lys-Lys-Tyr-Leu-Asn-Ser-Ile-Leu-Asn-NH2	N	Thr	Arg	Leu	Arg	Lys	Gln	Met	Ala	Roth, 2004
vasoactive intestinal peptide 10-28			Tyr-Thr-Arg-Leu-Arg-Lys-Gln-Met-Ala-Val-Lys-Lys-Tyr-Leu-Asn-Ser+Ile-Leu-Asn-NH2	N	Tyr	Leu	Asn	Ser	Ile	Leu	Asn	NH2	Roth, 2004