Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 25 |
matrix metallopeptidase-2 |
12-39 |
N |
|
MS |
Schilling & Overall, 2008 |
| 39 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 55 |
lysyl endopeptidase (bacteria) |
1-2511 |
N |
|
MS |
|
| 112 |
cathepsin L |
102-122 |
N |
|
MS |
Biniossek et al., 2011 |
| 202 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 213 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 241 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 257 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 261 |
elastase-2 |
257-274 |
N |
|
MS |
Schilling & Overall, 2008 |
| 262 |
cathepsin L |
258-274 |
N |
|
MS |
Biniossek et al., 2011 |
| 274 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 278 |
cathepsin L |
275-298 |
N |
|
MS |
Biniossek et al., 2011 |
| 278 |
cathepsin B |
275-299 |
N |
|
MS |
Biniossek et al., 2011 |
| 286 |
matrix metallopeptidase-2 |
274-298 |
N |
|
MS |
Schilling & Overall, 2008 |
| 287 |
meprin beta subunit |
284-298 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 287 |
LAST_MAM peptidase (Limulus-type) |
284-298 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 287 |
cathepsin G |
274-298 |
N |
|
MS |
Schilling & Overall, 2008 |
| 289 |
HIV-1 retropepsin |
274-298 |
N |
|
MS |
Schilling & Overall, 2008 |
| 298 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 330 |
meprin beta subunit |
327-343 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 373 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 384 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 455 |
meprin beta subunit |
452-468 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 456 |
glutamyl endopeptidase I |
443-468 |
N |
|
MS |
Schilling & Overall, 2008 |
| 468 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 531 |
matrix metallopeptidase-2 |
525-543 |
N |
|
MS |
Schilling & Overall, 2008 |
| 543 |
glutamyl endopeptidase I |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 619 |
matrix metallopeptidase-2 |
611-631 |
N |
|
MS |
Schilling & Overall, 2008 |
| 631 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 646 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 655 |
LAST_MAM peptidase (Limulus-type) |
652-667 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 666 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 667 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 685 |
HIV-1 retropepsin |
676-699 |
N |
|
MS |
Schilling & Overall, 2008 |
| 689 |
meprin alpha subunit |
686-700 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 690 |
elastase-2 |
676-700 |
N |
|
MS |
Schilling & Overall, 2008 |
| 699 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 700 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 790 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 802 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 807 |
matrix metallopeptidase-2 |
802-825 |
N |
|
MS |
Schilling & Overall, 2008 |
| 813 |
elastase-2 |
802-825 |
N |
|
MS |
Schilling & Overall, 2008 |
| 814 |
matrix metallopeptidase-2 |
802-825 |
N |
|
MS |
Schilling & Overall, 2008 |
| 825 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 860 |
matrix metallopeptidase-2 |
835-883 |
N |
|
MS |
Schilling & Overall, 2008 |
| 868 |
LAST_MAM peptidase (Limulus-type) |
865-883 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 869 |
astacin |
866-883 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 883 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1007 |
HIV-1 retropepsin |
997-1019 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1019 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1043 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1051 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1089 |
cathepsin S |
1086-1098 |
N |
|
MS |
Biniossek et al., 2011 |
| 1093 |
cathepsin B |
1086-1104 |
N |
|
MS |
Biniossek et al., 2011 |
| 1104 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1107 |
meprin beta subunit |
1104-1116 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 1116 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1119 |
cathepsin L |
1117-1131 |
N |
|
MS |
Biniossek et al., 2011 |
| 1119 |
astacin |
1116-1131 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 1131 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1141 |
peptidyl-Lys metallopeptidase |
1-2511 |
N |
|
MS |
|
| 1142 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1157 |
peptidyl-Lys metallopeptidase |
1-2511 |
N |
|
MS |
|
| 1158 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1171 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1180 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1198 |
HIV-1 retropepsin |
1187-1208 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1200 |
matrix metallopeptidase-2 |
1187-1208 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1207 |
peptidyl-Lys metallopeptidase |
1-2511 |
N |
|
MS |
|
| 1208 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1211 |
glutamyl endopeptidase I |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1212 |
HIV-1 retropepsin |
1208-1225 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1217 |
matrix metallopeptidase-2 |
1208-1225 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1224 |
peptidyl-Lys metallopeptidase |
1-2511 |
N |
|
MS |
|
| 1225 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1229 |
glutamyl endopeptidase I |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1233 |
glutamyl endopeptidase I |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1238 |
peptidyl-Lys metallopeptidase |
1-2511 |
N |
|
MS |
|
| 1239 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1246 |
cathepsin L |
1242-1253 |
N |
|
MS |
Biniossek et al., 2011 |
| 1410 |
LAST_MAM peptidase (Limulus-type) |
1407-1423 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 1429 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1435 |
meprin beta subunit |
1432-1444 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 1444 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1476 |
matrix metallopeptidase-2 |
1470-1495 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1478 |
cathepsin L |
1471-1495 |
N |
|
MS |
Biniossek et al., 2011 |
| 1478 |
cathepsin S |
1471-1495 |
N |
|
MS |
Biniossek et al., 2011 |
| 1480 |
elastase-2 |
1470-1495 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1495 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1507 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1515 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1525 |
meprin beta subunit |
1522-1538 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 1582 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1591 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1606 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1742 |
HIV-1 retropepsin |
1739-1752 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1752 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1758 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1851 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1855 |
cathepsin L |
1852-1866 |
N |
|
MS |
Biniossek et al., 2011 |
| 1866 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1934 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1953 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1970 |
matrix metallopeptidase-2 |
1958-1981 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1976 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1981 |
glutamyl endopeptidase I |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1986 |
glutamyl endopeptidase I |
1976-1995 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1995 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 2015 |
matrix metallopeptidase-2 |
2007-2026 |
N |
|
MS |
Schilling & Overall, 2008 |
| 2026 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 2193 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 2206 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 2220 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 2223 |
cathepsin S |
2221-2233 |
N |
|
MS |
Biniossek et al., 2011 |
| 2223 |
meprin beta subunit |
2220-2233 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 2233 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 2245 |
matrix metallopeptidase-2 |
2233-2263 |
N |
|
MS |
Schilling & Overall, 2008 |
| 2263 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 2275 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 2373 |
cathepsin S |
2355-2382 |
N |
|
MS |
Biniossek et al., 2011 |
| 2396 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 2406 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
| 2461 |
granzyme B (Homo sapiens-type) |
1-2511 |
N |
|
MS |
Van Damme et al., 2010 |
| 2491 |
glutamyl endopeptidase I |
2482-2505 |
N |
|
MS |
Schilling & Overall, 2008 |
| 2492 |
HIV-1 retropepsin |
2482-2505 |
N |
|
MS |
Schilling & Overall, 2008 |
| 2492 |
matrix metallopeptidase-2 |
2482-2505 |
N |
|
MS |
Schilling & Overall, 2008 |
| 2505 |
trypsin 1 |
1-2511 |
N |
|
MS |
Schilling & Overall, 2008 |
